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1.
Sci Rep ; 13(1): 8179, 2023 05 20.
Artículo en Inglés | MEDLINE | ID: mdl-37210416

RESUMEN

Biophilic design based on indoor planting plays an important role in human physical and mental well-being. To investigate and assess the effects of indoor planting on air quality, we sequenced 16S rRNA gene amplicons to compare the airborne bacterial microbiomes of three planting rooms before and after installing natural materials (plants, soil, water, etc.) with distinct biophilic attributes. Incorporation of indoor plantings significantly increased the taxonomic diversity of the airborne microbiome in each room, and we observed different microbiome compositions in each room. The proportional contribution of each bacterial source to the airborne microbiome in the indoor planting rooms was estimated by SourceTracker2. This analysis revealed that the proportion of airborne microbial sources (e.g., plants and soil) varied depending on the natural materials installed. Our results have important implications for indoor planting with biophilic design to control the indoor airborne microbiome.


Asunto(s)
Contaminación del Aire Interior , Microbiota , Humanos , ARN Ribosómico 16S/genética , Microbiota/genética , Bacterias/genética , Plantas/genética , Suelo , Contaminación del Aire Interior/análisis , Microbiología del Aire
2.
Plant Physiol ; 190(3): 1731-1746, 2022 10 27.
Artículo en Inglés | MEDLINE | ID: mdl-35951755

RESUMEN

In plant stems, secondary vascular development is established through the differentiation of cylindrical vascular cambium, producing secondary xylem (wood) and phloem (bast), which have economic importance. However, there is a dearth of knowledge on the genetic mechanism underlying this process. NAC with Transmembrane Motif 1-like transcription factor 9 (NTL9) plays a central role in abiotic and immune signaling responses. Here, we investigated the role of NTL9 in vascular cambium development in Arabidopsis (Arabidopsis thaliana) inflorescence stems by identifying and characterizing an Arabidopsis phloem circular-timing (pct) mutant. The pct mutant exhibited enhanced vascular cambium formation following secondary phloem production. In the pct mutant, although normal organization in vascular bundles was maintained, vascular cambium differentiation occurred at an early stage of stem development, which was associated with increased expression of cambium-/phloem-related genes and enhanced cambium activity. The pct mutant stem phenotype was caused by a recessive frameshift mutation that disrupts the transmembrane (TM) domain of NTL9. Our results indicate that NTL9 functions as a negative regulator of cambial activity and has a suppressive role in developmental transition to the secondary growth phase in stem vasculature, which is necessary for its precise TM domain-mediated regulation.


Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Cámbium/metabolismo , Proteínas de Arabidopsis/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Xilema/genética , Xilema/metabolismo , Tallos de la Planta/genética , Tallos de la Planta/metabolismo , Regulación de la Expresión Génica de las Plantas
3.
Plant J ; 103(6): 2139-2150, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32579240

RESUMEN

We previously developed a large-scale genome restructuring technology called the TAQing system. It can induce genomic rearrangements by introducing transient and conditional formation of DNA double-strand breaks (DSBs) via heat activation of a restriction enzyme TaqI, which can cleave DNA at 5'-TCGA-3' sequences in the genome at higher temperatures (37-42°C). Such heat treatment sometimes confers lethal damage in certain plant species and TaqI cannot induce rearrangements in AT-rich regions. To overcome such problems we developed an extended TAQing (Ex-TAQing) system, which enables the use of a wider range of restriction enzymes active at standard plant-growing temperatures. We established the Ex-TAQing system using MseI that can efficiently cleave DNA at room temperature (at temperatures ranging from 22 to 25°C) and the 5'-TTAA-3' sequence which is highly abundant in the Arabidopsis genome. A synthetic intron-spanning MseI gene, which was placed downstream of a heat-shock-inducible promoter, was conditionally expressed upon milder heat treatment (33°C) to generate DSBs in Arabidopsis chromosomes. Genome resequencing revealed various types of genomic rearrangements, including copy number variations, translocation and direct end-joining at MseI cleavage sites. The Ex-TAQing system could induce large-scale rearrangements in diploids more frequently (17.4%, n = 23) than the standard TAQing system. The application of this system to tetraploids generated several strains with chromosomal rearrangements associated with beneficial phenotypes, such as high salinity stress tolerance and hypersensitivity to abscisic acid. We have developed the Ex-TAQing system, allowing more diverse patterns of genomic rearrangements, by employing various types of endonucleases and have opened a way to expand the capacity for artificial genome reorganization.


Asunto(s)
Edición Génica/métodos , Genoma de Planta/genética , Arabidopsis/genética , Arabidopsis/metabolismo , Roturas del ADN de Doble Cadena , ADN de Plantas/genética , ADN de Plantas/metabolismo , Desoxirribonucleasas de Localización Especificada Tipo II/metabolismo , Reordenamiento Génico/genética , Calor , Intrones/genética , Ploidias , Tetraploidía
4.
Cephalalgia ; 40(11): 1177-1190, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-32484063

RESUMEN

BACKGROUND: Cortical spreading depression is thought to be the underlying mechanism of migraine aura. In 2006, three relatives having the point mutation E700K in ATP1A2 exon 15 were diagnosed with familial hemiplegic migraine 2 characterized by complicated forms of aura. Here, we generated a transgenic mouse model having the human E700K mutation in the Atp1a2 orthologous gene. OBJECTIVE: To investigate the characteristics of cortical spreading depression in a mouse model with E700K mutation in the Atp1a2. METHODS: Cortical spreading depression was induced by applying stepwise increases of KCl concentration or electrical stimulation intensity to C57BL/6J-Tg(Atp1a2*E700K)9151Kwk mice (Tg, both sexes) and corresponding wild-type animals. Under urethane anesthesia, the responsiveness and threshold to cortical spreading depression were examined and the distribution of c-Fos expression, a neuronal activity marker, was immunohistochemically determined. RESULTS: Overall, Tg mice showed significantly faster propagation velocity (p < 0.01) and longer full-width-at-half-maximum (p < 0.01) than wild-type animals, representing a slower recovery from direct current potential deflection. The cortical spreading depression threshold tended to be lower in Tg, especially in females. c-Fos-positive cells were significantly enhanced in the ipsilateral somatosensory cortex, piriform cortex, amygdala and striatum (each p < 0.05 vs. contralateral side). Numbers of c-Fos positive cells were significantly higher in the ipsilateral amygdala of Tg, as compared with wild-type animals (p < 0.01). CONCLUSION: The effect of cortical spreading depression may be greater in E700K transgenic mice than that in wild-type animals, while the threshold for cortical spreading depression shows little change. Higher c-Fos expression in the amygdala may indicate alterations of the limbic system in Tg, suggesting an enhanced linkage between cortical spreading depression and amygdala connectivity in familial hemiplegic migraine 2 patients.


Asunto(s)
Depresión de Propagación Cortical/fisiología , Migraña con Aura/genética , Proteínas Proto-Oncogénicas c-fos/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/genética , Animales , Modelos Animales de Enfermedad , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Migraña con Aura/metabolismo , Migraña con Aura/fisiopatología , Mutación Puntual
5.
ACS Appl Mater Interfaces ; 12(19): 22298-22307, 2020 May 13.
Artículo en Inglés | MEDLINE | ID: mdl-32320201

RESUMEN

The structures of K or Cs alkaline-treated Cu(In,Ga)(S,Se)2 (CIGSSe) solar cells are developed, and their carrier recombination rates are scrutinized. It is determined that short-circuit current density (JSC) is enhanced (decreased optical loss), when ZnS(O,OH), (Cd,Zn)S, and Zn0.8Mg0.2O buffers with a large band gap energy (Eg) are applied as a replacement of CdS buffer. The JSC is further increased, reducing the optical loss more, when Zn0.9Mg0.1O:B is used as the transparent conductive oxide (TCO) with a larger Eg and lower free carrier absorption than those of ZnO:Al. Furthermore, all carrier recombination rates throughout the devices with K or Cs treatment, especially at the buffer/absorber interface and in the quasi neutral region, are reduced, thereby reducing open-circuit voltage deficit (VOC,def), well consistent with the simulated ones. The carrier recombination rate at the buffer/absorber interface is further decreased, when the CdS and (Cd,Zn)S buffers, deposited by chemical bath deposition, are applied, leading to the greater reduction of the VOC,def and the high conversion efficiency (η) of about 21%. Under the trade-off between VOC,def and optical loss, the highest η of 22.6% is attained with the lowest power loss (or the highest VOC × JSC) in the Cs-treated Cd-free CIGSSe solar cell with an optimized structure of glass/Mo/CIGSSe/Zn0.8Mg0.2O/Zn0.9Mg0.1O:B, fabricated by the all-dry process, where the Zn0.8Mg0.2O buffer is prepared by the sputtering method. This occurs because the JSC is the highest attributable to the larger Eg of Zn0.8Mg0.2O buffer than those of the CdS and (Cd,Zn)S.

6.
FEBS Open Bio ; 10(6): 1031-1043, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32237043

RESUMEN

The ATP1A2 coding α2 subunit of Na,K-ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+ ) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2tmCKwk/+ crossed with transgenic mice expressing G-CaMP7 in cortical neurons and astrocytes (Atp1a2+/- ), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/- were higher than those in wild-type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/- may contribute to FHM2 pathophysiology.


Asunto(s)
Astrocitos/metabolismo , Corteza Cerebral/patología , Depresión de Propagación Cortical/genética , Migraña con Aura/genética , ATPasa Intercambiadora de Sodio-Potasio/deficiencia , Animales , Calcio/análisis , Calcio/metabolismo , Cationes Bivalentes/análisis , Cationes Bivalentes/metabolismo , Corteza Cerebral/citología , Modelos Animales de Enfermedad , Femenino , Heterocigoto , Humanos , Microscopía Intravital , Ratones , Ratones Transgénicos , Microscopía de Fluorescencia por Excitación Multifotónica , Migraña con Aura/patología , Neuronas/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/genética , Técnicas Estereotáxicas
7.
Plant J ; 102(5): 1042-1057, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-31925982

RESUMEN

Plant cells have acquired chloroplasts (plastids) with a unique genome (ptDNA), which developed during the evolution of endosymbiosis. The gene content and genome structure of ptDNAs in land plants are considerably stable, although those of algal ptDNAs are highly varied. Plant cells seem, therefore, to be intolerant of any structural or organizational changes in the ptDNA. Genome rearrangement functions as a driver of genomic evolutionary divergence. Here, we aimed to create various types of rearrangements in the ptDNA of Arabidopsis genomes using plastid-targeted forms of restriction endonucleases (pREs). Arabidopsis plants expressing each of the three specific pREs, i.e., pTaqI, pHinP1I, and pMseI, were generated; they showed the leaf variegation phenotypes associated with impaired chloroplast development. We confirmed that these pREs caused double-stranded breaks (DSB) at their recognition sites in ptDNAs. Genome-wide analysis of ptDNAs revealed that the transgenic lines exhibited a large number of rearrangements such as inversions and deletions/duplications, which were dominantly repaired by microhomology-mediated recombination and microhomology-mediated end-joining, and less by non-homologous end-joining. Notably, pHinP1I, which recognized a small number of sites in ptDNA, induced drastic structural changes, including regional copy number variations throughout ptDNAs. In contrast, the transient expression of either pTaqI or pMseI, whose recognition site numbers were relatively larger, resulted in small-scale changes at the whole genome level. These results indicated that DSB frequencies and their distribution are major determinants in shaping ptDNAs.


Asunto(s)
Enzimas de Restricción del ADN/metabolismo , Plastidios/genética , Variaciones en el Número de Copia de ADN/genética , Variaciones en el Número de Copia de ADN/fisiología , Enzimas de Restricción del ADN/genética , Evolución Molecular , Genoma del Cloroplasto/genética , Genoma de Plastidios/genética
8.
iScience ; 23(12): 101817, 2020 Dec 18.
Artículo en Inglés | MEDLINE | ID: mdl-34095782

RESUMEN

The highest theoretical efficiency of double junction solar cells is predicted for architectures with the bottom cell bandgap (E g ) of approximately 0.9-1.0 eV, which is lower than that of a typical Si cell (1.1 eV). Cu(In,Ga)(Se,S)2 (CIGS) solar cells exhibit a tunable E g depending on their elemental composition and depth profile. In this study, various CIGS solar cells with E g ranging from 1.02 to 1.14 eV are prepared and a spectrum splitting system is used to experimentally demonstrate the effect of using lower-E g cells as the bottom cell of two-junction solar cells. The four-terminal tandem cell configuration fabricated using a mixed-halide perovskite top cell (E g  = 1.59 eV; stand-alone efficiency = 21.0%) and CIGS bottom cell (E g  = 1.02 eV; stand-alone efficiency = 21.5%) with a 775-nm spectral splitting mirror exhibits an efficiency of 28.0% at the aperture area of 1 cm2.

9.
J Vis Exp ; (143)2019 01 23.
Artículo en Inglés | MEDLINE | ID: mdl-30735178

RESUMEN

Gait disturbance is frequently observed in patients with movement disorders. In mouse models used for movement disorders, gait analysis is important behavioral test to determine whether the mice mimic the symptoms of patients. Motor deficits are often induced by stress when no spontaneous motor phenotype is observed in the mouse models. Therefore, gait analysis followed by stress loading would be a sensitive method for evaluating the motor phenotype in mouse models. However, researchers face the requirement of an expensive apparatus to obtain quantitative results automatically from gait analysis. For stress, stress loading by simple methods without expensive apparatuses required for electric shock and forced running is desirable. Therefore, we introduce a simple and low-cost protocol consisting of footprint analysis with paper and ink, hanging box test to evaluate motor function, and stress loading defined by restraint with a conical tube. The motor deficits of mice were successfully detected by this protocol.


Asunto(s)
Marcha , Actividad Motora/fisiología , Restricción Física , Animales , Modelos Animales de Enfermedad , Masculino , Ratones , Fenotipo , Restricción Física/métodos , Carrera
10.
ACS Appl Mater Interfaces ; 11(7): 7539-7545, 2019 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-30694052

RESUMEN

Cd-free Cu(In,Ga)(S,Se)2 (CIGSSe) solar cells with a structure of glass/Mo/CIGSSe/Zn1- xMg xO (buffer)/Zn1- xMg xO:Al (TCO), fabricated by an all dry process, are characterized using ultraviolet light excited time-resolved photoluminescence (UV-TRPL). The impact of bandgap energy ( Eg) values of buffer and transparent conductive oxide (TCO) layers, denoted by Eg of buffer and TCO, is examined. The Eg values of buffer and TCO layers are kept almost similar and varied from 3.30 to 3.94 eV. In this work, UV-TRPL measurement is performed to examine the UV-TRPL carrier lifetimes near the Zn1- xMg xO buffer/CIGSSe interface in the solar cell structure. It is revealed that the UV-TRPL carrier lifetimes near the Zn1- xMg xO buffer/CIGSSe interface in Cd-free solar cells are increased upon enhancing the Eg of buffer and TCO from 3.30 to 3.94 eV, thus increasing the open-circuit voltage and fill factor. Additionally, short-circuit current density is enhanced up to about 38 mA/cm2 owing to the highly transparent Zn1- xMg xO/Zn1- xMg xO:Al layers. Ultimately, an 18.5%-efficient Cd-free solar cell with the Eg of buffer and TCO of 3.94 eV, prepared by an all dry process, is fabricated, which has the same level of 18.3% for the reference solar cell (glass/Mo/CIGSSe/CdS/ZnO/ZnO:Al).

11.
Neurobiol Dis ; 118: 142-154, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30026035

RESUMEN

Astrocyte-microglia communication influences the onset and progression of central nervous system (CNS) disorders. In this study, we determined how chronic inflammation by activated astrocytes affected and regulated CNS functions in Sandhoff disease (SD), a CNS lysosomal storage disorder. SD triggers intense CNS inflammation such as microglial activation and astrogliosis. It is caused by mutation of the HEXB gene, which reduces ß-hexosaminidase (Hex) enzymatic activity in lysosomes, leading to accumulation of the substrate GM2 ganglioside in neuronal cells. Hexb-/- mice display a phenotype similar to human patients that suffer from chronic inflammation characterized by activation of astrocytes and microglia. In Hexb-/- mice, tremors and loss of muscle coordination begins at ~12 weeks. Interestingly, we found that reactive astrocytes expressed adenosine A2A receptor in the cerebral cortices of Hexb-/- mice at the later inflammatory phase. In cultured astrocytes, expression of A2A receptor could be induced by astrocyte defined medium, and then the activation of the A2A receptor induced ccl2 expression. In Hexb-/- mice, inhibition of the A2A receptor antagonized by istradefylline decreased the number of activated microglial cells and inflammatory cytokines/chemokines at 13 weeks. Thus, the astrocytic A2A receptor is an important sensor that regulates microglial activation in the late phase of inflammation.


Asunto(s)
Antagonistas del Receptor de Adenosina A2/farmacología , Astrocitos/metabolismo , Modelos Animales de Enfermedad , Microglía/metabolismo , Receptor de Adenosina A2A/metabolismo , Enfermedad de Sandhoff/metabolismo , Antagonistas del Receptor de Adenosina A2/uso terapéutico , Animales , Astrocitos/efectos de los fármacos , Células Cultivadas , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Microglía/efectos de los fármacos , Purinas/farmacología , Purinas/uso terapéutico , Enfermedad de Sandhoff/tratamiento farmacológico , Enfermedad de Sandhoff/genética
12.
Nat Commun ; 9(1): 1995, 2018 05 18.
Artículo en Inglés | MEDLINE | ID: mdl-29777105

RESUMEN

DNA double-strand break (DSB)-mediated genome rearrangements are assumed to provide diverse raw genetic materials enabling accelerated adaptive evolution; however, it remains unclear about the consequences of massive simultaneous DSB formation in cells and their resulting phenotypic impact. Here, we establish an artificial genome-restructuring technology by conditionally introducing multiple genomic DSBs in vivo using a temperature-dependent endonuclease TaqI. Application in yeast and Arabidopsis thaliana generates strains with phenotypes, including improved ethanol production from xylose at higher temperature and increased plant biomass, that are stably inherited to offspring after multiple passages. High-throughput genome resequencing revealed that these strains harbor diverse rearrangements, including copy number variations, translocations in retrotransposons, and direct end-joinings at TaqI-cleavage sites. Furthermore, large-scale rearrangements occur frequently in diploid yeasts (28.1%) and tetraploid plants (46.3%), whereas haploid yeasts and diploid plants undergo minimal rearrangement. This genome-restructuring system (TAQing system) will enable rapid genome breeding and aid genome-evolution studies.


Asunto(s)
Arabidopsis/genética , Roturas del ADN de Doble Cadena , Genoma Fúngico , Genoma de Planta , Saccharomyces cerevisiae/genética , Arabidopsis/metabolismo , Reparación del ADN , Diploidia , Reordenamiento Génico , Inestabilidad Genómica , Saccharomyces cerevisiae/metabolismo , Tetraploidía
13.
PLoS One ; 13(5): e0196834, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29742174

RESUMEN

Mouse ultrasonic vocalizations (USVs) are studied in many fields of science. However, various noise and varied USV patterns in observed signals make complete automatic analysis difficult. We improve several methods to reduce noise, detect USV calls and automatically cluster USV calls. After reduction of noise and detection of USV calls, we consider USV calls as functional data and characterize them as USV functions with B-spline basis functions. For discontinuous USV calls, breakpoints in the USV functions are defined using multiple knots in the construction of the B-spline basis functions, and a hierarchical method is used to cluster the USV functions by shape. We finally show the performance of the proposed methods with USV data recorded for laboratory mice.


Asunto(s)
Ratones/fisiología , Ondas Ultrasónicas , Vocalización Animal , Animales , Análisis por Conglomerados , Conjuntos de Datos como Asunto , Femenino , Masculino , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Conducta Sexual Animal , Conducta Social , Vocalización Animal/clasificación
14.
ACS Appl Mater Interfaces ; 10(13): 11361-11368, 2018 Apr 04.
Artículo en Inglés | MEDLINE | ID: mdl-29533596

RESUMEN

Development of Cd-free Cu(In,Ga)(S,Se)2 (CIGSSe)-based thin-film solar cells fabricated by an all-dry process is intriguing to minimize optical loss at a wavelength shorter than 520 nm owing to absorption of the CdS buffer layer and to be easily integrated into an in-line process for cost reduction. Cd-free CIGSSe solar cells are therefore prepared by the all-dry process with a structure of Zn0.9Mg0.1O:Al/Zn0.8Mg0.2O/CIGSSe/Mo/glass. It is demonstrated that Zn0.8Mg0.2O and Zn0.9Mg0.1O:Al are appropriate as buffer and transparent conductive oxide layers with large optical band gap energy values of 3.75 and 3.80 eV, respectively. The conversion efficiency (η) of the Cd-free CIGSSe solar cell without K-treatment is consequently increased to 18.1%. To further increase the η, the Cd-free CIGSSe solar cell with K-treatment is next fabricated and followed by posttreatment called the heat-light-soaking (HLS) + light-soaking (LS) process, including HLS at 110 °C followed by LS under AM 1.5G illumination. It is disclosed that the HLS + LS process gives rise to not only the enhancement of carrier density but also the decrease in the carrier recombination rate at the buffer/absorber interface. Ultimately, the η of the Cd-free CIGSSe solar cell with K-treatment prepared by the all-dry process is enhanced to the level of 20.0%.

15.
PLoS One ; 10(9): e0137764, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26389588

RESUMEN

Despite high estimates of the heritability of aggressiveness, the genetic basis for individual differences in aggression remains unclear. Previously, we showed that the wild-derived mouse strain MSM/Ms (MSM) exhibits highly aggressive behaviors, and identified chromosome 15 (Chr 15) as the location of one of the genetic factors behind this escalated aggression by using a panel of consomic strains of MSM in a C57BL/6J (B6) background. To understand the genetic effect of Chr 15 derived from MSM in detail, this study examined the aggressive behavior of a Chr 15 consomic strain towards different types of opponent. Our results showed that both resident and intruder animals had to have the same MSM Chr 15 genotype in order for attack bites to increase and attack latency to be reduced, whereas there was an intruder effect of MSM Chr 15 on tail rattle behavior. To narrow down the region that contains the genetic loci involved in the aggression-eliciting effects on Chr 15, we established a panel of subconsomic strains of MSM Chr 15. Analysis of these strains suggested the existence of multiple genes that enhance and suppress aggressive behavior on Chr 15, and these loci interact in a complex way. Regression analysis successfully identified four genetic loci on Chr 15 that influence attack latency, and one genetic locus that partially elicits aggressive behaviors was narrowed down to a 4.1-Mbp region (from 68.40 Mb to 72.50 Mb) on Chr 15.


Asunto(s)
Agresión/fisiología , Conducta Animal/fisiología , Cromosomas de los Mamíferos , Sitios Genéticos , Animales , Mapeo Cromosómico , Humanos , Masculino , Ratones
16.
Adv Mater ; 26(44): 7427-31, 2014 Nov 26.
Artículo en Inglés | MEDLINE | ID: mdl-25155874

RESUMEN

High-efficiency Cu2ZnSn(S,Se)4 solar cells are reported by applying In2S3/CdS double emitters. This new structure offers a high doping concentration within the Cu2ZnSn(S,Se)4 solar cells, resulting in a substantial enhancement in open-circuit voltage. The 12.4% device is obtained with a record open-circuit voltage deficit of 593 mV.

17.
J Exp Bot ; 65(18): 5385-400, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25038254

RESUMEN

In contrast to mammals, higher plants have evolved to express diverse protein phosphatase 2Cs (PP2Cs). Of all Arabidopsis thaliana PP2Cs, members of PP2C subfamily A, including ABI1, have been shown to be key negative regulators of abscisic acid (ABA) signalling pathways, which regulate plant growth and development as well as tolerance to adverse environmental conditions. However, little is known about the enzymatic and signalling roles of other PP2C subfamilies. Here, we report a novel Arabidopsis subfamily E PP2C gene, At3g05640, designated AtPP2CF1. AtPP2CF1 was dramatically expressed in response to exogenous ABA and was expressed in vascular tissues and guard cells, similar to most subfamily A PP2C genes. In vitro enzymatic activity assays showed that AtPP2CF1 possessed functional PP2C activity. However, yeast two-hybrid analysis revealed that AtPP2CF1 did not interact with PYR/PYL/RCAR receptors or three SnRK2 kinases, which are ABI1-interacting proteins. This was supported by homology-based structural modelling demonstrating that the putative active- and substrate-binding site of AtPP2CF1 differed from that of ABI1. Furthermore, while overexpression of ABI1 in plants induced an ABA-insensitive phenotype, Arabidopsis plants overexpressing AtPP2CF1 (AtPP2CF1oe) were weakly hypersensitive to ABA during seed germination and drought stress. Unexpectedly, AtPP2CF1oe plants also exhibited increased biomass yield, mainly due to accelerated growth of inflorescence stems through the activation of cell proliferation and expansion. Our results provide new insights into the physiological significance of AtPP2CF1 as a candidate gene for plant growth production and for potential application in the sustainable supply of plant biomass.


Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/crecimiento & desarrollo , Arabidopsis/metabolismo , Inflorescencia/crecimiento & desarrollo , Inflorescencia/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Biomasa , Proliferación Celular/fisiología , Regulación de la Expresión Génica de las Plantas , Inflorescencia/genética
18.
Behav Brain Res ; 272: 100-10, 2014 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-24983657

RESUMEN

Dystonia is a neurological disorder with involuntary and simultaneous contractions of agonist and antagonist muscles. Rapid-onset dystonia parkinsonism (RDP), one of the heredity forms of dystonia, is caused by mutations of Na,K-ATPase α3 subunit gene (ATP1A3). The abrupt onset of bulbar and limb symptoms of RDP are often triggered by physical and/or emotional stress. We reported previously that Atp1a3-deficient heterozygous mice showed higher locomotor activity and developed enhanced dystonia symptoms after kainate injection into the cerebellum, but not spontaneous movement disorder like RDP patients. Here we show that Atp1a3-deficient heterozygous mice exhibited shorter stride length at 4 weeks of age without stress and at later stages under chronic restraint stress loading. Shorter hanging time in the hanging box test was also observed after stress loading. Shorter stride length and hanging time may be relevant to certain phenotypes, such as gait abnormality, observed in RDP patients. Atp1a3 was widely expressed in the brain, including basal ganglia and cerebellum, and spinal cord of young mice, and the expression pattern was compatible with movement abnormalities under lack of one of alleles. Our results demonstrated the usefulness of Atp1a3-deficient heterozygous mice as an animal model of RDP and its potential use to explore the pathophysiology of movement abnormality in this disorder.


Asunto(s)
Trastornos Distónicos/fisiopatología , Actividad Motora/fisiología , ATPasa Intercambiadora de Sodio-Potasio/deficiencia , Estrés Psicológico/fisiopatología , Animales , Fenómenos Biomecánicos , Encéfalo/patología , Encéfalo/fisiopatología , Enfermedad Crónica , Modelos Animales de Enfermedad , Trastornos Distónicos/patología , Femenino , Heterocigoto , Masculino , Ratones Transgénicos , Restricción Física , Caracteres Sexuales , ATPasa Intercambiadora de Sodio-Potasio/genética , Médula Espinal/patología , Médula Espinal/fisiopatología , Estrés Psicológico/patología
19.
J Neurosci Methods ; 234: 127-34, 2014 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-24768576

RESUMEN

BACKGROUND: Owing to their complex nature, social interaction tests normally require the observation of video data by a human researcher, and thus are difficult to use in large-scale studies. We previously established a statistical method, a hidden Markov model (HMM), which enables the differentiation of two social states ("interaction" and "indifference"), and three social states ("sniffing", "following", and "indifference"), automatically in silico. NEW METHOD: Here, we developed freeware called DuoMouse for the rapid evaluation of social interaction behavior. This software incorporates five steps: (1) settings, (2) video recording, (3) tracking from the video data, (4) HMM analysis, and (5) visualization of the results. RESULTS: Using DuoMouse, we mapped a genetic locus related to social interaction. We previously reported that a consomic strain, B6-Chr6C(MSM), with its chromosome 6 substituted for one from MSM/Ms, showed more social interaction than C57BL/6 (B6). We made four subconsomic strains, C3, C5, C6, and C7, each of which has a shorter segment of chromosome 6 derived from B6-Chr6C, and conducted social interaction tests on these strains. DuoMouse indicated that C6, but not C3, C5, and C7, showed higher interaction, sniffing, and following than B6, specifically in males. COMPARISON WITH EXISTING METHOD: The data obtained by human observation showed high concordance to those from DuoMouse. The results indicated that the MSM-derived chromosomal region present in C6-but not in C3, C5, and C7-associated with increased social behavior. CONCLUSIONS: This method to analyze social interaction will aid primary screening for difference in social behavior in mice.


Asunto(s)
Mapeo Cromosómico , Variación Genética , Relaciones Interpersonales , Cadenas de Markov , Reconocimiento de Normas Patrones Automatizadas , Animales , Investigación Conductal/instrumentación , Investigación Conductal/métodos , Cromosomas Humanos Par 6/genética , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Sitios de Carácter Cuantitativo
20.
J Physiol ; 591(13): 3433-49, 2013 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-23652595

RESUMEN

Dystonia is characterized by excessive involuntary and prolonged simultaneous contractions of both agonist and antagonist muscles. Although the basal ganglia have long been proposed as the primary region, recent studies indicated that the cerebellum also plays a key role in the expression of dystonia. One hereditary form of dystonia, rapid-onset dystonia with parkinsonism (RDP), is caused by loss of function mutations of the gene for the Na pump α3 subunit (ATP1A3). Little information is available on the affected brain regions and mechanism for dystonia by the mutations in RDP. The Na pump is composed of α and ß subunits and maintains ionic gradients of Na(+) and K(+) across the cell membrane. The gradients are utilized for neurotransmitter reuptake and their alteration modulates neural excitability. To provide insight into the molecular aetiology of RDP, we generated and analysed knockout heterozygous mice (Atp1a3(+/-)). Atp1a3(+/-) showed increased symptoms of dystonia that is induced by kainate injection into the cerebellar vermis. Atp1a3 mRNA was highly expressed in Purkinje cells and molecular-layer interneurons, and its product was concentrated at Purkinje cell soma, the site of abundant vesicular γ-aminobutyric acid transporter (VGAT) signal, suggesting the presynaptic localization of the α3 subunit in the inhibitory synapse. Electrophysiological studies showed that the inhibitory neurotransmission at molecular-layer interneuron-Purkinje cell synapses was enhanced in Atp1a3(+/-) cerebellar cortex, and that the enhancement originated via a presynaptic mechanism. Our results shed light on the role of Atp1a3 in the inhibitory synapse, and potential involvement of inhibitory synaptic dysfunction for the pathophysiology of dystonia.


Asunto(s)
Corteza Cerebelosa/fisiología , Distonía/fisiopatología , ATPasa Intercambiadora de Sodio-Potasio/fisiología , Animales , Técnicas In Vitro , Interneuronas/fisiología , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Actividad Motora , Neuronas/fisiología , Subunidades de Proteína/fisiología , Desempeño Psicomotor , Transmisión Sináptica
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