RESUMEN
We collected 204 nondiarrhoeic canine fecal samples and isolated 68 Clostridium difficile strains from 62 of these samples. Strains were grouped into 29 PCR ribotypes. Only 47% of the strains were toxigenic.
Asunto(s)
Clostridioides difficile/aislamiento & purificación , Perros/microbiología , Animales , Clostridioides difficile/clasificación , Heces/microbiología , Femenino , Japón , Masculino , Proyectos Piloto , Reacción en Cadena de la Polimerasa/veterinaria , Ribotipificación/veterinariaRESUMEN
The biomimetic synthesis of the advanced model compound of chloropupukeananin has been achieved. The present synthesis features an unexpected enantiomer-differentiating Diels-Alder/carbonyl-ene cascade under high-pressure conditions and a base-promoted migration of the salicyl group.
Asunto(s)
Sesquiterpenos/síntesis química , Biomimética , Modelos Moleculares , Estructura Molecular , Salicilatos/química , Sesquiterpenos/química , EstereoisomerismoRESUMEN
Bisaminal cyclization in the zoanthamine alkaloid system was strongly affected by the stereochemistry of the C4 methyl. While cyclization of the (4S)-methyl precursor gave only a bisaminal compound, cyclization of the (4R)-methyl isomer produced both spiroketal and bisaminal products.
