Comparative Analysis of Stress-Protective Activity and Prevention of Fatigue during Physical Load of Standardized Humic Acids from Peat and Officinal Preparations of Succinic Acid in Experiment.

The effect of humic acids and substances with similar action - derivatives of succinic acid (ethylmethylhydroxypyridine succinate) and combined agent consisting of succinic acid, nicotinamide, riboflavin, and riboxin on the performance and stress resistance of experimental rats was studied. Performance was assessed in the test of exhaustive forced swimming with a load, stress resistance was evaluated by the serum level of corticosterone and open field behavior, and the state of anaerobic metabolism was estimated by the serum level of lactate after swimming test. Humic acids from peat showed anti-stress activity comparable to that of the officinal preparation and preventive effect on fatigue during physical exercise. They can be recommended as a component for the development of drugs that increase human performance and stress resistance.

[Comparison of neurotropic and antioxidant activity of Cytoflavin and etilmetilgydroxypiridine succinat in patients with chronic brain ischemia]. / Sravnitel'naya kharakteristika neirotropnoi i antioksidantnoi aktivnosti Tsitoflavina i etilmetilgidroksipiridina suktsinata u patsientov s khronicheskoi ishemiei golovnogo mozga.

OBJECTIVE: Determination of the total antioxidant activity of blood serum (AOA) of patients with chronic cerebral ischemia (CCI) I-II st. against the background of treatment with Cytoflavin or ethylmethylhydroxypyridine succinate (EMGPS) and evaluation of the effect of drugs on the state of cognitive functions and emotional sphere of patients. MATERIAL AND METHODS: The main group consisted of 12 women with CCI I-II st., treated on an outpatient basis with Cytoflavin 1 tablet 2 times/day as part of complex therapy for 1 month; 12 patients with CCI I-II st., treated as part of complex therapy with EMGPS 125 mg 2 times/day for 1 month we made a comparison group. The groups are comparable in age, gender, the nature of risk factors, the severity of neurological manifestations, and the nature of basic therapy. The state of AOA, the severity and nature of cognitive and emotional disorders were assessed. RESULTS: It was found that the inclusion of Cytoflavin or EMGPS in complex therapy equally increased the AOA of patients. The decrease in the severity of neurological deficit during treatment consisted in improving spatio-temporal gnosis by improving the relationships in the cortex and synchronizing the activity of its different departments with the rate of nervous activity and neurophysiological characteristics different for each drug. The use of both drugs led to a decrease in the selection reaction time and the number of errors in recognizing the angular velocity of movement. Cytoflavin increased the selectivity of perception and attention in patients, while EMGPS did not affect this indicator. CONCLUSION: The use of Cytoflavin and EMGPS leads to an increase in AOA, has various effects on the cognitive functions of patients with CCI.

Fitting the determined impedance in the guinea pig inner ear to Randles circuit using square error minimization in the range of 100 Hz to 50 kHz.

Objective. Several lumped and distributed parameter models of the inner ear have been proposed to improve vestibular implant stimulation. The models should account for all significant physical phenomena that influence the current propagation, such as the electrical double layer (EDL) and medium polarization. The electrical properties of the medium are reflected in the electrical impedance; therefore, the study aimed to measure the impedance in the guinea pig inner ear and construct its equivalent circuit.Approach. The electrical impedance was measured from 100 Hz to 50 kHz between a pair of platinum electrodes immersed in 0.9% NaCl saline solution using sinusoidal voltage signals. The Randles circuit was fitted to the measured impedance in the saline solution in order to estimate the EDL parameters (C,W,andRct) of the electrode interface in saline. Then, the electrical impedance was measured between all combinations of the electrodes located in the semicircular canal ampullae and the vestibular nerve in the guinea pigin vitro. The extended Randles circuit considering the medium polarization (Ri,Re,Cm) together with EDL parameters (C,Rct) obtained from the saline solution was fitted to the measured impedance of the guinea pig inner ear. The Warburg element was assumed negligible and was not considered in the guinea pig model.Main results. For the set-up used, the obtained EDL parameters were:C=27.09*10-8F,Rct=18.75kΩ.The average values of intra-, extracellular resistances, and membrane capacitance wereRi=4.74kΩ,Re=45.05kΩ,Cm=9.69*10-8F,respectively.Significance. The obtained values of the model parameters can serve as a good estimation of the EDL for modelling work. The EDL, together with medium polarization, plays a significant role in the electrical impedance of the guinea pig inner ear, therefore, they should be considered in electrical conductivity models to increase the credibility of the simulations.

[Effectiveness and safety of mexidol forte 250 in the sequential therapy in patients with chronic ischemia]. / Effektivnost' i bezopasnost' preparata Meksidol FORTE 250 pri posledovatel'noi terapii patsientov s khronicheskoi ishemiei mozga.

OBJECTIVE: To study of efficacy and safety of mexidol used as intravenous infusion for 14 days, followed by per os treatment with mexidol FORTE 250 for 60 days in patients with chronic brain ischemia (CHM) complicated with arterial hypertension and atherosclerosis. MATERIAL AND METHODS: The mexidol group included 27 patients (24 women and 3 men) with CHM I-II gr and the combination of arterial hypertension and atherosclerosis who received intravenous infusions of mexidol (500 mg once daily) within 14 days, with the subsequent per os treatment with mexidol FORTE 250 in a daily dose of 750 mg (1 tablet 3 times a day) for 60 days. The comparison group consisted of 30 patients (22 women and 8 men) with CHM I-II gr, comparable in age, nature of risk factors and expression of neurological manifestations. Patients in both groups received basic medications to treat their risk factors. Motor activity (Tinetti test), cognitive functions (MoCa test), anxiety and depression (Hamilton anxiety and depression scale), clinical condition (General Clinical Impression scale) were assessed. RESULTS AND CONCLUSION: Inclusion of mexidol (500 mg iv infusion once a day within 14 days with the subsequent oral administration of 750 mg (1 tablet 3 times a day) for 60 days) in standard therapy of arterial hypertension with atherosclerosis and chronic brain ischemia is expedient. The results show greater clinical efficacy and sufficient safety of such combination therapy. By the end of therapy (day 74), patients in the mexidol group have a reliable improvement in motor activity, cognitive function and psychoemotional sphere, as well as a decrease in fatigue and neurological manifestations compared with the comparison group.

[An effect of mexidol on the level of corsticosteron in the blood serum and beam zone cells in rats with light desynhronizes and physical loads]. / Vliyanie meksidola na uroven' kortikosterona v syvorotke krovi i kletki puchkovoi zony kory nadpochechnikov u krys pri svetovykh desinkhronozakh i fizicheskikh nagruzkakh.

OBJECTIVE: In order to develop methods and means of maintaining normal human functioning under conditions of desynchronizes, the effect of mexidol on the level of corticosterone in the blood serum and the state of the cells of the beam zone of the adrenal cortex of rats after light or dark deprivation and physical activity to a state of fatigue. MATERIALS AND METHODS: The experiments were performed on 7 groups of rats (10 animals each) in the spring. The model of physical activity is the method of forced swimming of rats until exhaustion in its own modification. For the induction of experimental desynchronizes, the animals of the experimental groups were kept around the clock for 10 days with artificial bright light of 150 LX or a complete darkening of 2-3 LX. Mexidol was administered intramuscularly at a dose of 10 mg/kg animal 30 minutes before the swimming test. Control animals under similar conditions were administered 0.9% sodium chloride solution. The level of corticosterone in the serum was determined using enzyme immunoassay. The measurements were carried out on a programmable photometer for STAR FAX 303 PLUS microsamples (USA). Histological analysis of the adrenal glands was carried out according to standard methods. Microscopy of preparations, imaging and measurements were carried out using an Axio Lab A1 microscope, an AxioCamERc 5s camera and ZEN 2012 software («Carl Zeiss Microscopy¼, Germany). Statistical processing of the results was carried out using standard parametric and non-parametric methods, depending on the nature of the distributions. RESULTS AND CONCLUSION: Mexidol under combined successive stress loads - desynchronizes and physical activity - demonstrated anti-stress properties not only at the level of corticosterone in the blood serum of rats, but also at the level of adrenal glands. The severity of this effect of the drug depended on the number of stress loads (isolated physical or shown in conditions of desynchronizes), the phase of stress and the nature of deprivation. Under natural lighting conditions and after light deprivation, when animals were in the anxiety phase of varying severity, the drug worked more reliably than after dark deprivation in conditions of developing depletion. Nevertheless, even in the latter case, he showed himself as a means of preventing exhaustion in animals and the breakdown of adaptation.

Effect of Humic Acids from Lowland Peat on Endurance of Rats in Forced Swim Test in Relation to Serum Lactate and Corticosterone.

The study substantiated the possibility of using peat humic acids for improving endurance during extreme physical exertion. The mature outbred Wistar rats weighing 200-250 g (n=40) were subjected to forced swim test until complete exhaustion. The humic acids (1%) were administered intragastrically (0.5 ml/100 g body weight) 30 min prior to the test. Chronic administration of peat humic acids for 5 days increased physical capacity and endurance of rats in exhaustive forced swim test without the changes in serum lactate and corticosterone.

[Effect of mexidol on physical working capacity and level of lactate in the blood of rats in conditions of light deprivation]. / Vliianie meksidola na fizicheskuiu rabotosposobnost' i uroven' laktata v krovi krys v usloviiakh svetovykh desinkhronozov.

AIM: To study an effect of mexidol on the performance of rats after light or dark deprivations in the swimming test with a load and to evaluate the state of glycolytic processes under these conditions. MATERIAL AND METHODS: The experiment was carried out in the spring on 70 Wistar male rats. Three groups (30 animals) were in natural light conditions. One of them was not affected. The other two groups were subjected to exercise and 30 minutes before it either saline or mexidol was administered intramuscularly. Four other groups (40 animals) for 10 days were under conditions of dark or light deprivation prior to the presentation of physical activity and received either saline or mexidol before the test after deprivation was canceled. A forced swimming test with an additional load, which was presented to animals every day at 10-11 am for five days in a row, was used as a model of physical activity. The level of lactate was determined by colorimetric method. RESULTS AND CONCLUSION: Mexidol increased the performance of rats in the swimming test, both under natural lighting conditions and with light desynchronization, contributed to the formation of cross adaptation to physical activity under natural lighting conditions and prolonged this state under conditions of light deprivation, did not change the content of lactate in the blood of rats after exercise in natural lighting conditions and dark deprivation and prevented its rise after light deprivation.

Electrophysiological Properties of Rat Vestibular Labyrinth and Their Effect on Parameters of Transmitted Voltage Pulses.

We propose a new approach to optimization of electrical stimulation of the vestibular nerve and improving the transfer function of vestibular implant. A mathematical model of the vestibular organ is developed based on its anatomy, the model premises, 3D-analysis of MRI and CT images, and mathematical description of physical processes underlying propagation of alternating electric current across the tissues of vestibular labyrinth. This approach was tested in vitro on the rat vestibular apparatus and had been examined anatomically prior to the development of its mathematical model and equivalent electrical circuit. The experimental and theoretical values of changes of the gain-phase characteristics of vestibular tissues in relation to location of the reference electrode obtained in this study can be used to optimize the electrical stimulation of vestibular nerve.

[Gender aspects of medico-social adaptation in adults with early onset of epilepsy]. / Gendernye aspekty mediko-sotsial'noi adaptatsii u vzroslykh pri rannem debiute épilepsii.

AIM: To study the relationship between indicators of clinical picture and social adaptation in idiopathic and symptomatic epilepsies with onset before adulthood depending on patient's gender. MATERIAL AND METHODS: The cross-sectional study was carried out. The study group included 212 women and 171 men, aged 24-60 years, with confirmed diagnosis of epilepsy with onset before 18 years. Seventy-three patients were diagnosed with symptomatic epilepsy, 310 with idiopathic epilepsy. In 120 patients, the frequency of seizures was rarer than once a year. All patients had secondary education and were on treatment with antiepileptic drugs. RESULTS AND CONCLUSION: In symptomatic epilepsy with early onset, gender differences in family and educational status were not identified. Regardless of gender, patients with myoclonus had higher education more frequently than patients with absence and tonic-clonic seizures. Patients with the combination of different types of seizures, irrespective from etiology and gender, had secondary education more frequently. Women with rare generalized seizures more frequently had higher education and were married. Unmarried men with rare generalized seizures lived separately from their relatives more frequently. To author's opinion, the contradiction in indicators of social adaptation in men with rare generalized seizures, to the great extent, is related to the phenomenon of self-stigmatization than to the influence of disease. The results can be used in rehabilitation of patients with idiopathic epilepsy syndromes.

[SEASONAL FEATURES OF SEROTONIN IN BLOOD SERUM OF RATS AFTER PHYSICAL OVERWORK UNDER DESYNCHRONOSIS].

The content of serotonin in the blood serum of rats with light desynchronizes and physical fatigue in different seasons was investigated ву enzyme immunoassay. It was found that long-term light deprivation and exercise to complete depletion lowered levels of serotonin in the blood serum of rats in the winter and increased that of the spring season. At the same time, a dark deprivation and physical activity had no effect on this indicator.

[Reactivity of the central nervous system influences the sensitivity of secretory cells of the stomach to carbacholine and glycyl-proline (Gly-Pro)].

In experiments using cluster analysis of the behavioral activity of rats in the "open field" test, the animals were divided into three groups with different types of behavior and response to the first presentation of the test: (i) active-search (all the studied reactions are expressed), (ii) intermediate, and (iii) passive-defensive (lowest level of activity). Differences in the indices of carbacholine-stimulated gastric secretion in rats of different groups were manifested by greater secretion of the stomach and lower digestive capacity of gastric juice observed in rats with active-search and intermediate types of behavior in the "open field" test in contrast to rats with passive-defensive type. Combined administration of cholinomimetic and glycyl-proline (Gly-Pro) lead to decreased proteolytic activity of gastric juice in rats with active-search behavior in the "open field" test, decreased volume of gastric secretion in rats with an intermediate type of behavior, and increased volume of juice and its decreased digestive ability in rats with passive-defensive behavior.

[Changes in EEG parameters in sleep and wakefulness in adult patients with drug-resistant localization-related epilepsy].

A study included 113 patients with localization-related epilepsy treated with antiepileptic drugs: 53 patients with drug-resistant (uncontrolled) form of the disease and 60 patients with controlled epileptic seizures. A comparison group included 53 patients with rare seizures not taking antiepileptic drugs. In the group of patients with uncontrolled localization-related epilepsy, the EEG monitoring of the sleep-wake cycle revealed more frequent regional interictal epileptiform discharges and increased α- and Β-activities during sleep compared to wakefulness. In patients with controlled seizures, there were the circadian changes in intra- and inter-hemispheric coherent relations in θ- и Β-bands. These relations remained stable when the sleep phase was changed to the wake phase.

[Gamma-activity of the cerebral cortex: communication with intelligence and accuracy of perception of time].

Connection of indicators of intelligence and accuracy of perception of time is established with level cortical interactions on frequency of a gamma rhythm, and also phase parities between a gamma rhythm and low-frequency components EEG. Character of these communications differs at young men and girls, depends on a frequency range of a gamma rhythm, a kind and a stage of carried out activity.

A visual analog of mismatch negativity when stimuli differ in duration.

Event-linked brain potentials were studied in ten essentially healthy volunteers (six men, four women) aged 18-24 years. Subjects were presented with rare deviant and frequent standard visual stimuli in the standard odd-ball paradigm in conditions of active attention to stimulation and in conditions of distracted attention. Differences between deviant stimuli (50, 100, and 150 msec) and standard (200 msec) stimuli ranged from 50 to 150 msec. Negative difference waves (deviant stimulus with distracted attention minus control stimulus of the same duration) were seen at differences of 100 and 150 msec and these were most marked in the right temporal cortex 200-400 msec from stimulus onset. As this wave was seen in conditions of distracted attention, it appears to be a visual analog of mismatch negativity and reflects the process of the automatic detection of stimuli with deviant durations.

[Visual analog of mismatch negativity when stimuli differ in the duration].

Event-related potentials (ERPs) were studied in 10 practically healthy volunteers (6 male, 4 female) aged from 18 to 24 years during presentation of rare deviant and frequent standard visual stimuli in a standard odd-ball paradigm under conditions of active attention and inattention to stimulus duration. The deviation of the duration of deviant stimuli (50, 100 and 150 ms) from that of the standard stimuli (200 ms) varied from 50 to 150 ms. In series where deviations were 100 and 150 ms, a negative difference wave with the latency of 200-400 ms was revealed (ERP to the deviant stimulus under conditions of inattention minus ERP to the control stimulus of the same duration) mainly in the right temporal region. Since this difference negative wave appeared under conditions of inattention, we suppose that this wave is the visual analogue of mismatch negativity (MMN) and related to automatic detection of deviation of stimulus duration.