Safety, tolerability, and acceptability of Lactobacillus crispatus CTV-05 (LACTIN-V) in pregnant women at high-risk of preterm birth.

The vaginal microbiota is a determinant for the risk of preterm birth (PTB). Dominance of the vaginal niche by Lactobacillus crispatus associates with term delivery. This is the first observational clinical study of live vaginal biotherapeutics (Lactobacillus crispatus CTV-05 (LACTIN-V)) in pregnant women at high-risk of PTB. The primary aim was to explore safety, tolerability and acceptability of LACTIN-V in pregnancy. Women were offered a course of LACTIN-V at 14 weeks gestation for five consecutive days followed by weekly administration for six weeks. Participants were followed up at 15, 18-, 20-, 28- and 36-weeks' gestation and at delivery for assessment of adverse events, compliance and tolerability. Participants completed a questionnaire to gauge experience and acceptability. In total, 73 women were recruited, of whom eight withdrew, leaving a final cohort size of 61. Self-reported compliance to the course was high (56/60, 93%). Solicited adverse events were reported in 13 women (19%) including changes in vaginal discharge, odour, colour or consistency of urine, itching and vaginal bleeding. One unsolicited adverse event was reported as haematuria at 38 weeks gestation, but was judged to be unrelated to LACTIN-V. No serious adverse events occurred. One mild adverse event led to study withdrawal. Thirty-one women completed an experience and acceptability questionnaire. Women found LACTIN-V easy and comfortable to use and the majority (30/31, 97%) would use LACTIN-V in future pregnancies. Eight women (8/31, 26%) found the schedule of use difficult to remember. The rate of PTB <34 weeks in this cohort was 3.3% compared to 7% in a historical cohort of 2,190 women at similar background PTB risk. With satisfactory uptake and good compliance, we demonstrate that LACTIN-V is safe and accepted in pregnancy, with high tolerability. Further studies are needed to assess colonisation of Lactobacillus crispatus CTV-05 and clinical efficacy.

Experiences of Multidisciplinary Working: Perspectives from the Wessex Ghana Stroke Partnership.

INTRODUCTION: Substantial gaps remain in our understanding of stroke in Africa as well as in stroke care, practice and policy on the continent. The effective organization of preventative, therapeutic and rehabilitative stroke services continue to be challenging in many African countries. METHODOLOGY: In this article we define the nature, function and benefits of effective multidisciplinary team (MDT) working. The experiences and perspectives of members of the MDT were collated by focus group discussions as well as individual and country specific contributions. RESULTS: The experiences and perspectives of multidisciplinary team members from the United Kingdom and Ghana implementing these practices at the first stroke unit in Korle Bu Teaching Hospital, Accra, with a transparent discussion of successes and challenges faced throughout development of the service, is presented. MDT working has improved outcomes for patients and families who use the services, including encouraging better shared treatment planning and compliance. More stroke rehabilitation services are provided than previously, including greater self-management education and better secondary prevention care. CONCLUSION: It is hoped that this article will provide an inspirational model for others working to provide stroke care in low-resource settings in Africa and worldwide.

INTRODUCTION: Des lacunes substantielles subsistent dans notre compréhension de l'accident vasculaire cérébral en Afrique ainsi que dans les pratiques et politiques sur le continent. L'organisation efficace des services de prévention, de traitement et de réadaptation des AVC reste un défi dans de nombreux pays africains. MÉTHODOLOGIE: Dans cet article, nous définissons la nature, la fonction et les avantages d'une équipe multidisciplinaire efficace (PCT).Les expériences et les points de vue des membres de la PCT ont été recueillis par des discussions de groupes de réflexion ainsi que contributions individuelles et spécifiques à chaque pays. RÉSULTATS: Les expériences et les perspectives des membres d'équipes multidisciplinaires du Royaume-Uni et du Ghana, qui ont mis en œuvre ces pratiques dans la première unité d'AVC du de l'hôpital universitaire Korle Bu, à Accra, avec une discussion transparente des succès et des défis rencontrés tout au long du développement du service, sont présentées. Le travail de la PCT a amélioré les résultats pour les patients et les familles qui utilisent les services, notamment en encourageant un meilleur partage du traitement, l'organisation et la complaisance. Davantage de services de réadaptation après un AVC sont fournis plus qu'auparavant, y compris une meilleure éducation à l'autogestion et de meilleurs soins de prévention secondaire. CONCLUSION: Nous espérons que cet article constituera un modèle inspirant pour d'autres personnes travaillant à la prise en charge d'AVC dans des contextes à faibles ressources en Afrique et dans le monde. Mots clés: Unité de expériences, multidisciplinaire, Ghana, partenariat.

The effects of hydrochloric acid on all-ceramic restorative materials: an in-vitro study.

OBJECTIVES: The aim of this in-vitro study was to assess the long-term effects of hydrochloric acid on the surface roughness of three all-ceramic restorative materials CEREC VITABLOC Mark II CAD, IPS Empress CAD and IPS e.max CAD. MATERIALS AND METHODS: Six cylindrical specimens (10mm diameter, 3mm height) of each material type were prepared, using the CEREC CAD/CAM machine. The unpolished samples were immersed in 15ml hydrochloric acid (pH 2) at 37 degrees C. Before immersion (baseline) and at periods of 7.5 hours, 45 hours and 91 hours, the specimens were removed from the acid and two randomised areas (10 microm X 10 microm) were selected and tested on each. The atomic force microscope (Bruker Dimension icon) was used to assess surface roughness and surface area at baseline and after each exposure time. The materials were compared over time with respect to surface roughness and surface area (baseline, 1 month, 6 months, 1 year) in a repeated measures analysis of variance (ANOVA). RESULTS: Sample groups differed significantly for roughness (p < 0.0001) and surface area (p < 0.0001). For both parameters a significant interaction also existed between material and time (surface roughness: p = 0.0085; surface area: p = 0.0014). CEREC VITABLOC Mark II CAD and IPS Empress CAD had substantially higher levels of roughness and surface area than IPS e.max CAD, which was also affected to a lesser extent over time. CONCLUSION: The results showed that IPS e.max CAD was least affected by long-term exposure to hydrochloric acid.

A comparison of preload values in gold and titanium dental implant retaining screws.

This in vitro investigation compared the effect of using either gold or titanium retaining screws on preload in the dental implant- abutment complex. Inadequate preload can result in screw loosening, whilst fracture may occur if preload is excessive. These are the most commonly reported complications in implant-retained prostheses, and result in unscheduled, costly and time-consuming visits for the patient and the clinician. This study investigated changes in preload generation after repeated torque applications to gold and titanium screws. The test set-up consisted of an implant body, a cylindrical transmucosa abutment, and the test samples of gold and of titanium retaining screws. The implant bodies were anchored using a load cell, and the transmucosal abutments were attached using either gold or titanium retaining screws. A torque gauge was used to apply torque of 20Ncm, 32Ncm, and 40Ncm to the retaining screws. The preloads generated in each screw type were compared at each torque setting, and after repeated tightening episodes. In addition, the effect of applying torque beyond the manufacturers' recommendations was also examined. Gold retaining screws were found to achieve consistently higher preload values than titanium retaining screws. Preload values were not significantly different from the first to the tenth torque cycle. Titanium screws showed more consistent preload values, albeit lower than those of the gold screws. However due to possible galling of the internal thread of the implant body by titanium screws, gold screws remain the retaining screw of choice. Based on the findings of this study, gold retaining screws generate better preload than titanium. Torque beyond the manufacturers' recommendations resulted in a more stable implant complex. However, further investigations, with torque applications repeated until screw breakage, are needed to advise on ideal maintenance protocols.

Evaluation of surface characteristics of titanium and cobalt chromium implant abutment materials.

INTRODUCTION: Micro-organism adhesion and plaque formation is affected by surface free energy (SFE), surface roughness, hydrophilicity, surface chemistry, surface charge and the presence of proteins. AIMS: The aim of this study was to assess and compare surface characteristics of surgical grade cobalt chromium alloy (CCM) and of commercially pure titanium (cpTi). METHOD: Nine metallic cylinders were machined to precise standards from each material. Surface roughness was measured at four different points on each sample and the average Ra value was calculated for each material. Contact angles were obtained using the sessile-drop method and applied in calculating the SFE. Surface hardness was evaluated by means of a Vickers hardness micro-indentation. RESULTS: Surface roughness was similar for both metals, but total SFE values and Vickers surface hardness scores showed significant differences (p < 0.0001). CONCLUSION: SFE analysis showed CCM to be more hydrophobic and that oral bacteria might therefore be less adherent than to cpTi. The mean Vickers Hardness scores of the cpTi were significantly lower (p < 0.0001), suggesting that CCM may be more resistant to surface modifications and surface roughening, thus remaining smoother with less plaque accumulation than cpTi. This study demonstrated that CCM might be a suitable alternative implant abutment material.

Students' vulnerability in educational research.

Dental teaching institutions in South Africa recently implemented "learner-centred" curricula and expected educators to alter their teaching styles accordingly, but perhaps without providing adequate training in this paedagogical philosophy. At the same time, the lecturers were required to conduct evidence-based research to evaluate the outcomes. Thus, clinicians/lecturers also became researchers, using their own students or student material for assessment purposes. Previously, this form of educational research, which was carried out in normal academic settings, was not subject to review by Institutional Review Boards (IRB). However, concerns have risen that learners may be a vulnerable population due to their position in the academic institution, and the power and knowledge differentials that exist between them and the lecturer/researcher. This raises ethical concerns regarding their autonomy and ability to provide free, voluntary, informed consent to be research participants. This paper questions whether educational research may lead to student vulnerability, and proposes some recommendations for educators and institutions involved in educational research.

The scope of maxillofacial prosthodontics.

Oral cancer may affect up to 275 000 new patients per year worldwide. Many of these will be disfigured by the destruction of tissue within the face and head area. Maxillofacial prosthodontics can play a vital role in restoring such patients to a semblance of normality in appearance and function. This article will describe the role of maxillofacial prosthodontics in the treatment of these oral cancer patients.

A simple method to ensure sufficient occlusal reduction in fixed prosthodontics.

Tooth preparations for full coverage restorations must ensure there is adequate occlusal clearance to accommodate the restorative material, in both static and functional occlusion. The shape of the prepared occlusal surface should also be contoured to ensure that the created "space to restore" is evenly distributed so that the restoration will be of a uniform thickness. Many of the methods described in the literature are difficult to use clinically, especially in posterior lingual and palatal regions. This article describes how a modification of the wax wafer technique can be used to identify the exact areas of the tooth that need reduction in order to achieve the desired clearance.

Vitamin D deficiency and supplementation during pregnancy.

OBJECTIVE: Vitamin D is essential for skeletal health and prolonged deficiency results in infantile rickets and adult osteomalacia. The aim of this study is to determine the vitamin D status in pregnancy and to evaluate the effects of daily and of single-dose vitamin D supplementation. DESIGN: A prospective randomized study at St Mary's Hospital London. PATIENTS: A total of 180 women (Indian Asian, Middle Eastern, Black and Caucasian) were recruited at 27 weeks gestation and randomized into three treatment groups: a single oral dose of 200,000 IU vitamin D, a daily supplement of 800 IU vitamin D from 27 weeks until delivery and a no treatment group. MEASUREMENTS: Vitamin D (25-hydroxyvitamin D), PTH and corrected calcium levels in mothers at 27 weeks and at delivery and cord 25-hydroxyvitamin D and corrected calcium levels. RESULTS: The final maternal 25-hydroxyvitamin D levels were significantly higher in the supplemented group [daily dose (median) 42 (IQR 31-76) nmol/l, stat dose (median) 34 (IQR 30-46) nmol/l vs. median 27 (IQR 27-39) nmol/l in the no treatment; P < 0.0001] and significantly fewer women with secondary hyperparathyroidism in the supplemented group (10% in daily dose vs. 12% in stat dose vs. 27% in the no treatment; P < 0.05). Cord 25-hydroxyvitamin D levels were significantly higher with supplementation [daily dose median 26 (IQR 17-45) nmol/l, stat dose median 25 (IQR 18-34) nmol/l vs. median 17 (IQR 14-22) nmol/l in no treatment; P = 0.001]. CONCLUSION: Single or daily dose improved 25-hydroxyvitamin D levels significantly. However, even with supplementation, only a small percentage of women and babies were vitamin D sufficient. Further research is required to determine the optimal timing and dosing of vitamin D in pregnancy.

The accuracy of three impression transfer techniques for implant supported prostheses.

Cast framework for osseointegrated implant retained prostheses need to be passively fitting so as not to place undue forces and stress concentrations around the fixtures. Impression materials and techniques play a key role in the accuracy and fit of the metal framework. In this study, three impression procedures were investigated to establish which was the most accurate, reliable and predictable. This included using smooth sided tapered transfer copings and unsplinted and splinted square undercut transfer copings. A metal plated master model was fabricated and used as the standard against which models poured using the different impression techniques were compared. Readings were taken with a Reflex Microscope and used to calculate the mean, standard deviation and deviation index for each test model. The results of this study suggest that direct coping transfer techniques provide comparable or better results than indirect coping transfers in master cast fabrication. There was no significant difference in impression transfer accuracy between splinted and non-splinted square impression coping transfer techniques.

A comprehensive review of mercury provoked autism.

Emerging evidence supports the theory that some autism spectrum disorders (ASDs) may result from a combination of genetic/biochemical susceptibility, specifically a reduced ability to excrete mercury (Hg), and exposure to Hg at critical developmental periods. Elemental/inorganic Hg is released into the air/water where it becomes methylated and accumulates in animal tissues. The US population is primarily exposed to methyl-Hg by fish consumption. In addition, many pharmaceuticals have been, and some continue to be, a ubiquitous source of danger because they contain mercurials. Mercurials may be found in drugs for the eye, ear, nose, throat, and skin; in bleaching creams; as preservatives in cosmetics, tooth pastes, lens solutions, vaccines, allergy test and immunotherapy solutions; in antiseptics, disinfectants, and contraceptives; in fungicides and herbicides; in dental fillings and thermometers; and many other products. Hg has been found to cause immune, sensory, neurological, motor, and behavioural dysfunctions similar to traits defining/associated with ASDs, and that these similarities extend to neuroanatomy, neurotransmitters, and biochemistry. Furthermore, a review of molecular mechanisms indicates that Hg exposure can induce death, disorganization and/or damage to selected neurons in the brain similar to that seen in recent ASD brain pathology studies, and this alteration may likely produce the symptoms by which ASDs are diagnosed. Finally, a review of treatments suggests that ASD patients who undergo protocols to reduce Hg and/or its effects show significant clinical improvements in some cases. In conclusion, the overwhelming preponderance of the evidence favours acceptance that Hg exposure is capable of causing some ASDs.

Dentine hypersensitivity: a review of its aetiology, pathogenesis and management.

Dentine hypersensitivity (DH) is defined as pain arising from exposed dentine and represents a distinct clinical entity. Reported cases are increasing particularly among the younger age groups and are thought to be due to acidogenic diets, destructive habits, poor tooth brushing techniques, and the increased use of tooth whitening products. Dentine exposure may be due to a number of processes, both physical and chemical, that lead to either loss of enamel/ cementum or loss of gingival tissue. These causative factors seldom act in isolation and include erosion, abrasion, attrition, abfraction, bruxing, bleaching, medication, ageing, genetic conditions, gingival recession, and periodontal disease or procedures. There are diverse range of treatment products available, which aim at either occluding the dentinal tubules or blocking the neural transmission from the pulp. Most reversible options make use of chemical agents such as fluorides, oxalate, strontium or potassium salts, or dentine-bonding agents. Non-reversible options should only be employed after one or more of the reversible options have been attempted. These usually involve placement of permanent restorations, occlusal adjustments or periodontal flap surgery. Careful diagnosis, patient counseling and management strategies are crucial to the success of any intervention.

Accuracy of the Raypex-4 and Propex apex locators in detecting horizontal and vertical root fractures: an in vitro study.

Unforeseen root fractures during endodontic therapy are often difficult to diagnose and treat. Apex locators have been shown to be accurate in measuring the working lengths of root canals, and it was postulated whether they could also be used to determine the position of root fractures. This study was undertaken to assess the accuracy of two different apex locators in determining the position of fractures. One hundred single rooted teeth were randomly divided into two groups. One group had simulated horizontal fractures cut into them and the other group had vertical fractures. All fractures were detected in both groups using both a Propex (third generation) and a Raypex-4 (fourth generation) apex locator. The actual lengths of the fractures were then measured under 2.5 times magnification, and the results subjected to statistical analysis. Both locators produced similar results and were found to be very accurate, with measurements that correlated closely to the actual lengths. Clinically, treatment options for root fractures vary depending on their location. Thus apex locators may be a valuable aid in not only determining the presence of a root fracture, but also its exact location, which will help the clinician decide on the most appropriate management.