Dense macrophyte cover has significant structural and functional influence on planktonic microbial communities leading to bacterial success.

We intend to assess how macrophyte cover affects planktonic microbial communities by changing the physical and chemical environment, and how macrophyte-derived DOC affects the balance between autotrophy and heterotrophy/chemoorganotrophy in a shallow lake. The structure and production of phytoplankton and bacterioplankton in the open water of a large shallow lake and in the littoral zone were compared at two sampling stations with different macrophyte cover. According to the obtained results, uncoupling between bacterioplankton and phytoplankton was observed due to the high content of organic carbon of emergent macrophyte origin. While phytoplankton were regulated by TSS, bacterioplankton (in both heterotrophic and photoheterotrophic forms) were determined by dissolved organic carbon. As a result of these processes, the littoral and pelagic zones in the lake are completely separated from each other. In open water the autotrophic processes dominated, but at the sampling stations inside the reed belt, the metabolic processes shifted in the direction of chemoorganotrophy. Our results suggest that increase of macrophyte cover in shallow water bodies will increase the significance of microbe-based carbon pathways and weakens the efficiency of carbon transport from primary producers to higher trophic levels through the planktonic food chain.

Grazing pressure-induced shift in planktonic bacterial communities with the dominance of acIII-A1 actinobacterial lineage in soda pans.

Astatic soda pans of the Pannonian Steppe are unique environments with respect to their multiple extreme physical and chemical characteristics (high daily water temperature fluctuation, high turbidity, alkaline pH, salinity, polyhumic organic carbon concentration, hypertrophic state and special ionic composition). However, little is known about the seasonal dynamics of the bacterial communities inhabiting these lakes and the role of environmental factors that have the main impact on their structure. Therefore, two soda pans were sampled monthly between April 2013 and July 2014 to reveal changes in the planktonic community. By late spring in both years, a sudden shift in the community structure was observed, the previous algae-associated bacterial communities had collapsed, resulting the highest ratio of Actinobacteria within the bacterioplankton (89%, with the dominance of acIII-A1 lineage) ever reported in the literature. Before these peaks, an extremely high abundance (> 10,000 individuum l-1) of microcrustaceans (Moina brachiata and Arctodiaptomus spinosus) was observed. OTU-based statistical approaches showed that in addition to algal blooms and water-level fluctuations, zooplankton densities had the strongest effect on the composition of bacterial communities. In these extreme environments, this implies a surprisingly strong, community-shaping top-down role of microcrustacean grazers.

Aerobic anoxygenic phototrophs are highly abundant in hypertrophic and polyhumic waters.

Aerobic anoxygenic phototrophs (AAPs) are a group of photoheterotrophic bacteria common in natural waters. Here, AAP abundance and contribution to total bacterial abundance and biomass were investigated to test whether the trophic status of a lake or content of coloured dissolved organic matter (CDOM) play a role in determining AAP distribution and abundance in shallow inland lakes, with special focus on hypertrophic and polyhumic waters. Twenty-six different shallow lakes in Hungary were monitored. AAP abundance and biomass were determined by epifluorescence microscopy. The lakes exhibit a broad range of CDOM (2-7000 mg Pt L-1) and phytoplankton biomass (2-1200 µg L-1 chlorophyll a concentration). Very high AAP abundance (up to 3 × 107 cells mL-1) was observed in polyhumic and hypertrophic shallow lakes. AAP abundance was influenced by phytoplankton biomass and CDOM content, and these effects were interrelated. As determined, 40 µg L-1 chlorophyll a and 52 mg Pt L-1 CDOM are threshold levels above which these effects have a synergistic relationship. Hence, the observed high AAP abundance in some soda pans is a consequence of combined hypertrophy and high CDOM content. AAP contribution was influenced by total suspended solids (TSS) content: the success of AAP cells could be explained by high TSS levels, which might be explained by the decrease of their selective grazing control.