Response to anti-SARS-CoV-2 mRNA vaccines in multiple myeloma and chronic lymphocytic leukemia patients.

Multiple myeloma (MM) and chronic lymphocytic leukemia (CLL) patients have increased morbidity and mortality rates of COVID-19 due to immunosuppression associated with the disease and ongoing therapy. The same immune impairment accompanying CLL and MM also affects suboptimal vaccine response. The study assessed the effectiveness of the humoral and T cell-mediated immunity following mRNA COVID-19 vaccination (using either BNT162b2 or mRNA-1273) in short-term (2-5 weeks after second dose) and long-term follow-up (12 weeks after vaccination). Between March and August 2021, blood samples were obtained from 62 CLL and 60 MM patients from eight different hematology departments in Poland. Total anti-RBD antibodies were detected in 37% MM patients before vaccination, increased to 91% and 94% in short- and long-term follow-up, respectively. In CLL, serological responses were detectable in 21% of patients before vaccination and increased to 45% in the short-term and 71% in long-term observation. We detected a tendency to higher frequencies of specific CD8+ T cells against SARS-CoV-2 after vaccination compared to samples before vaccination in MM patients and no changes in frequencies of specific T cells in CLL patients. Our study provides novel insights into mRNA vaccination efficacy in immunocompromised MM and CLL patients, and our findings highlight that specific CD8+ T cells against SARS-CoV-2 might be induced by vaccination but do not correlate positively with serological responses.

The impact of time, environmental conditions, and sex on the degradation of bloody fingerprints on a glass surface.

Dactyloscopy is the branch of forensics that deals with identifying individuals by analyzing the configurations of the friction ridges on the fingertips. The persistence and degradation of fingerprints depends on such factors as the individual's sex and BMI (body mass index) and the duration and conditions of their preservation. There is a great deal of information on how the passage of time affects the image of fingerprints composed of sebum and sweat, but little knowledge concerning the preservation of bloody fingerprints. The aim of the study was to determine the effect of sex, the state of the blood forming the print (dry vs. fresh), environmental factors (room vs. outdoor conditions), and time on the width of friction ridge impressions in fingerprints. Fingerprints made with fresh blood were found to persist longest, while prints left by women and exposed to outdoor conditions degraded most rapidly. The ambient temperature also had a significant effect on the width of friction ridge impressions and their degradation.

Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress.

Little is known on the protective effects of L-proline on hen erythrocytes. The aim of the study was to determine the protective effects of this amino acid at concentrations of 50 µg/mL, 100 µg/mL, 200 µg/mL in hen erythrocytes subjected to temperatures 41 °C, 43 °C and 45 °C for 1 h and 4 h. The following cellular parameters were determined: viability, morphological alterations, caspase 3/7 activity, heat shock protein HSP70 1A activity and glutathione level. The results showed that exposure to 43 °C and 45 °C resulted in a decrease of viability and increased morphological alterations of the non-treated erythrocytes. Caspase 3/7 activity was increased only at 45 °C, however HSP70 1A activity and glutathione level were increased in the temperature-dependent manner. On the other hand, erythrocytes additionally exposed to L-proline showed alterations of the parameters when compared to the non-treated cells. L-proline at 50 µg/mL and 100 µg/mL increased caspase 3/7 activity at both 41 °C and 43 °C, however it was less augmented at all the concentrations at 45 °C. Glutathione level was decreased in heat-stressed (at 43 °C and 45 °C) hen erythrocytes treated with L-proline (at 50 µg/mL and 100 µg/mL) but it was increased at 200 µg/mL. HSP70 1A activity was augmented in a concentration- and temperature-dependent manner. The results indicate that proapoptotic or antiapoptotic effects of L-proline depend on its concentration and temperature of heat stress and thermoprotective effects induced by the amino acid on some parameters in hen erythrocytes may be a result of stimulation of antioxidative defense and stimulation of HSP70 1A activity.

Short-term effects of pesticide fipronil on behavioral and physiological endpoints of Daphnia magna.

Fipronil (FIP) is an organic pesticide with many practical uses. Although some results indicated toxic effects in some terrestrial and aquatic animal species, little is known on its influence on behavioral and physiological endpoints of cladocerans. The aim of our study was to determine the short-term effects of FIP at concentrations of 0.1 µg/L, 1 µg/L, 10 µg/L, and 100 µg/L on Daphnia magna sublethal indices: behavioral (swimming speed, distance traveled) and physiological endpoints (heart rate, post-abdominal claw activity and thoracic limb movements). The results showed that FIP induced reduction of swimming speed and distance traveled in a concentration- and time-dependent manner at all the concentrations used. The lowest concentration of the insecticide temporarily stimulated post-abdominal claw activity after 24 h and thoracic limb activity after 48 h; however, the highest concentrations reduced all the studied physiological endpoints. IC50 values showed that thoracic limb activity, swimming speed, and distance traveled were most sensitive to FIP after 24-h exposure. The most sensitive parameter after 48 h and 72 h was swimming speed and post-abdominal claw activity, respectively. The study indicated that (i) behavioral and physiological endpoints of Daphnia magna are reliable and valuable sublethal indicators of toxic alterations induced by FIP; however, they respond with different sensitivity at various times of exposure, (ii) FIP may alter cladoceran behavior and physiological processes at concentrations detected in the aquatic environment; therefore, it should be considered as an ecotoxicological hazard to freshwater cladocerans.

Behavioral and physiological responses of Daphnia magna to salicylic acid.

Salicylic acid (SA), a metabolite of acetylsalicylic acid is a monohydroxybenzoic acid a common non-steroidal analgesic and anti-inflammatory drug (NSAID) frequently detected in various aquatic ecosystems at concentrations up to 19.50 µg L-1 in surface waters near livestock farms and 59.6 µg L-1 in wastewaters. Little is known on the effects of short-term exposure of freshwater crustaceans to salicylic acid. Therefore, the aim of our study was to determine the effects of SA at concentrations of 5 µg L-1, 500 µg L-1, 5 mg L-1, 50 mg L-1 and 500 mg L-1 on the behavior (swimming speed, swimming height, distance travelled) and physiological endpoints (heart rate, mandible movement) of Daphnia magna exposed for 24 h, 48 h and 72 h. The results showed that SA inhibited the swimming speed, swimming height and distance travelled, heart rate and mandible movement at 5 mg L-1, 50 mg L-1 and 500 mg L-1 when compared to the control. On the other hand, SA at 5 µg L-1 and 500 µg L-1 transiently increased swimming speed and distance travelled after 24 h of the exposure, except for swimming height. Behavioral and physiological disturbances were observed much earlier than lethality. Our study showed SA at environmental levels may be an ecotoxicological agent imparing behavior and physiology of freshwater crustaceans.

Early morphological and apoptotic responses of bird erythrocytes to thermal stress.

Little is known about early indicators of heat stress in bird erythrocytes. We investigated the effects of elevated temperatures on the morphology and cellular responses of avian erythrocytes. Hen red blood cells were subjected to 22-45 °C temperatures for 1 h and 4 h, then stained and examined by light microscopy to assess morphological alterations. Cell viability, cytotoxicity and caspases 3 and 7 activity also were investigated. We found that short-term exposure of hen blood to 43-45 °C caused morphological alterations and increased the activity of pro-apoptotic caspases 3 and 7; hemolytic cells also were found. Reduction of erythrocytes may be a consequence of direct disruption of the cell membrane, although apoptotic disintegration also may occur. Because changes in erythrocyte morphology were rapid, they may be useful indicators of thermal stress in birds.

Changes in Fingerprints and the Quantity of Material Forming the Print Depending on Hand Cleanliness, Gender, and Ambient Conditions.

Impressions of friction ridges left on a surface are important evidence for identifying an individual and can be used to confirm his or her presence at the scene of an incident. Factors influencing the durability of fingerprints include the physiological characteristics and cleanliness of the individual, environmental factors, and time. The aim of the study was to determine the effect of atmospheric factors, gender, hand cleanliness and time on the width of friction ridge impressions and the amount of material forming the print. The research shows that factors such as gender, ambient conditions, and hand cleanliness affect the width of the ridge impressions and the quantity of material forming the print. The passage of time significantly reduces both the width of the ridge impressions and the quantity of the material forming the print.

Effects of L-proline on swimming parameters of Daphnia magna subjected to heat stress.

L-proline (L-PROL) is an essential amino acid, a constituent of many proteins and the osmoprotective molecule produced and accumulated in higher plants and some freshwater microalgae in response to various environmental stressors. Knowledge on thermoprotective effects of this amino acid on freshwater invertebrates is very scarce. Therefore the aim of our study was to determine the effect of L-PROL at concentrations: 10 mg/L, 20 mg/L and 50 mg/L on swimming behavior (immobilization, swimming track density, swimming speed, turning ability) of Daphnia magna subjected to temperatures: 22 °C, 35 °C and 38 °C. We found that L-PROL elevated all the measured swimming parameters at 22 °C when compared to the untreated crustaceans. Furthermore, L-PROL alleviated heat-induced inhibition of these parameters in the experimental animals subjected to 35 °C. The results suggest that L-PROL stimulates swimming performance and alleviates alterations of swimming parameters induced by heat stress in D. magna. Moreover, these findings may support the hypothesis that in natural conditions, L-PROL may protect crustaceans against thermal stress.

Inhalation poisoning with palytoxin from aquarium coral: case description and safety advice.

Palythoa spp. corals and some other marine organisms contain one of the most poisonous substances ever known - palytoxin (PTX). Due to their modest life requirements and ease of breeding, these corals are popular in home aquariums. Here we refer to a case of PTX poisoning of a middle-aged woman who inhaled poisonous vapours while brushing the corals from live rock and compare it with the available literature. As the case revealed that the symptoms of PTX poisoning are not specific and neither is treatment, our aim was to give a brief tabulated review of the symptoms that may indicate such poisoning. Cases of palytoxin poisoning have been reported worldwide, and severe ones (mostly due to ingestion of contaminated sea food) can end in death. As it appears, most (if not all) poisonings result from unawareness of the risk and reckless handling by aquarists. This is one of the first articles which provides some practical advice about the use of personal protection equipment, including gloves, masks, eyewear, and other clothing during any coral manipulation to minimise the risk. We also draw attention to the lack of marketing/trading regulations for dangerous coral species and/or regulations or instructions dealing with their removal and health protection.

Changes in Fingerprints Depending on Physiological Factors.

The aim of the study was to determine the effect of gender, body mass index, and time on the width of friction ridge impressions in fingerprints and the content of sweat and sebum forming the print. The research showed that the friction ridge impressions of women are significantly wider than those of men, and this tendency persists throughout the existence of the print. The differences may result from the anatomical structure of the skin, as well as from the use of protective products such as creams, which are used more often by women. The width of friction ridge impression is similar in underweight and overweight people, but greater than in those with normal body weight, and the amount of sweat and sebum increases with body weight. The passage of time significantly reduces both the width of the friction ridge impression and the content of sweat and sebum secretions in the print.

Protective effects of compatible solute ectoine against ethanol-induced toxic alterations in Daphnia magna.

Ectoine (ECT) is a compatible solute synthesized mostly by halophilic microorganisms subjected to various stressful factors. Its protective properties in bacteria and some populations of isolated cells subjected to different stressors are reported; however, little is known on its effects against a commonly used compound, ethanol (ETH). The purpose of our study was to determine the effects of ETH alone (at 20 and 60 g/L) and in the combination with various concentrations of ECT (5, 10, and 25 mg/L) at various times of exposure on behavioural, physiological, and biochemical parameters of a model invertebrate Daphnia magna. In the present study, we determined the following parameters: immobilisation, heart rate, thoracic limb movement, catalase (CAT) activity, and nitric oxide species (NOx) level. Our study revealed that both concentrations of ETH alone induced immobilisation and decrease of swimming velocity, heart rate, and thoracic limb activity; however, catalase activity and NOx levels were increased. On the other hand, the animals exposed to the combinations of ETH + ECT showed a reduced immobilisation and alleviated inhibition of heart rate and thoracic limb activity, lower increase of CAT activity, and NOx level when compared to the crustaceans subjected to ETH alone. The most distinct alleviation of toxic effects was noted in the combinations in which the highest concentration of ECT were used. The results suggest that ETH may induce oxidative stress in daphnids and attenuating effects of ECT probably result from its antioxidative properties.