Effects of Different Tissue Adhesives in Treating Calvarial Bone Defects.

The purpose of this study is to investigate the potential of the 2 different tissue adhesive materials as a barrier for guided bone regeneration and to compare them with resorbable collogen membrane on autogenous grafted critical-sized cortical bone defects. Twenty-four rats were divided into 3 groups: Group M, Group G, and Group B. A 5-mm diameter critical-size defect was created in the calvarium of each animal. All defects were filled by autogenous graft harvested from tibia. In group M, resorbable collogen membrane was applied to defect area. In groups G and B, defects were closed by Glubran2 and Bioglue. All animals were euthanized at 28 days postoperative. Stereologic analyses were performed. New bone area and connective tissue volumes were measured. Mean new bone volumes were 0.97 ±â€Š0.28, 0.59 ±â€Š0.2, and 0.8 ±â€Š0.32 mm in groups M, G, and B, respectively. The differences between groups were not statistically significant (P > 0.05). In group M, mean connective tissue volumes were 4.25 ±â€Š0.7 mm, in groups G and B, 2.9 ±â€Š1.82 and 4.54 ±â€Š0.64 mm. Connective tissue volume differences were not statistically significant between groups. Glubran2 and Bioglue may play a role as a barrier for guided bone regeneration in treatment of critical size defects.

The Local Effect of Puerarin on Critical-Sized Calvarial Defects.

Pueraria, the root of Pueraria lobata, is a commonly used herb in alternative medicine. This study evaluates the effect of puerarin and autogenous graft material combination on bone regeneration in calvarial critical-sized bone defects. Twenty-four rats were randomly divided into 3 groups of 8 rats each. A 5-mm diameter critical-sized defect was created in the calvarium of each animal. In group C, defects were left unfilled and were allowed to heal spontaneously without the use of any grafting material. Critical-sized bone defect created in animals receiving no treatment. In group ABG, defects were filled with autologous bone graft only. In group P-ABG, defects were filled with autologous bone graft and puerarin combination. All animals were euthanized at 28 days postoperative. Stereologic analyses were performed. New bone area and connective tissue volumes were measured. Stereologic analysis showed that the difference between grafted groups (P-ABG and ABG) and group C was statistically significant with a mean bone formation of 1.13 ±â€Š0.09, 1.11 ±â€Š0.11, and 0.31 ±â€Š0.09 mm respectively (P ≤0.05). The connective tissue volumes were also statistically higher in groups P-ABG and ABG (P ≤0.05). Puerarin has positive effect on new bone formation in autogenous grafted critical-sized bone defects.

Effect of Local Rosuvastatin Administration on Calvarial Bone Defects.

The purpose of this study is to investigate the potential of the local administration of different doses of rosuvastatin (RSV) on autogenous grafted critical-sized cortical bone defects. Twenty-four rats were divided into 3 groups: Group C (control), Group RSV-0.1, and Group RSV-1. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by autogenous graft and sterile saline-treated absorbable collagen sponge (ACS) was applied. Defects in the experimental groups (groups RSV-0.1 and RSV-1) were grafted by autogenous graft and ACS with saline solution containing 0.1- and 1-mg RSV were applied. All animals were euthanized at 28 days after operation. Stereologic and micro-computed tomography (µCT) analyses were performed. New bone area and connective tissue volumes were measured. Stereologic analysis showed that the difference between group RSV-1 with a mean bone formation of 1.79 ±â€Š0.06 mm and groups RSV-0.1 and control (C) was statistically significant (P ≤ 0.05) with a mean bone formation of 1.29 ±â€Š0.28 mm and 1.08 ±â€Š0.12 mm, respectively. Connective tissue volume was also significantly higher in 1-mg RSV applicated group. Micro-CT results were similar with stereologic analyses. Local administered 1-mg RSV enhances bone regeneration in critical-size calvarial rat defects filled with autogenous graft.

The Effect of Local Rosuvastatin on Mandibular Fracture Healing.

Mandibular fracture is the most common injury seen in facial fractures and plays an important role for oral and maxillofacial surgery department. The purpose of this study is to investigate the potential of the local administration of rosuvastatin (RSV) on mandibular fracture healing in rats. Thirty-two rats were divided into 4 groups: group C-14 (control), group R-14, group C-28 (control), and group R-28. A unilateral standard vertical osteotomy was performed right side of the mandibula extending from the tooth to the mandibular basis for each animal. In groups C-14 and C-28 sterile saline treated absorbable collogen sponge was applied to the fracture area, in groups R-14 and R-28 absorbable collogen sponge with saline solution containing 1 mg RSV was applied to the fracture area. Animals in groups C-14 and R-14 were euthanized on the 14th day, groups C-28 and R-28 were euthanized on the 28th day after operation. Stereologic analyses were performed. New bone area and connective tissue volumes were measured. Stereologic analysis showed that group R-14 had significantly more new bone at 2 weeks compared with group C-14. Connective tissue volumes were also significantly higher in R-14. New bone and connective tissue volume differences were not statistically significant between groups C-28 and R-28. Locally administered RSV enhances early bone regeneration on mandibular fracture in rats.

Radiological and Stereological Evaluation of the Effect of Rifampin on Bone Healing in Critical-Size Defects.

OBJECTIVES: This study aimed to investigate the effect of rifampin with autogenous bone on bone regeneration in critical-size defects in the calvaria of rats. MATERIALS AND METHODS: In total, 40 rats were divided into 4 groups and a 5-mm diameter of calvarial defect was made in each rat's calvarium. Control group (C), bone defects were irrigated with sterile saline; rifampin group (R), bone defects were irrigated with rifampin. In the autogenous graft group (Ag), the autogenous graft was contaminated with saliva, and the defects were filled with an autogenous graft. In the autogenous graft + rifampin group (Ag+R), the autogenous graft was contaminated with saliva and was decontaminated with rifampin, and the defects were filled with the autogenous graft. The animals were killed at 4weeks. Bone formation was assessed by micro-computed tomography scanning and stereological analyses. RESULTS: The mean new bone volume was the greatest in the Ag/rifampin group (1.73 ±â€Š0.17), followed by the Ag group (1.50 ±â€Š0.05) (statistically significant difference at P < 0.05). The new bone volume was the lowest in the control group (1.05 ±â€Š0.09); however, no difference was observed compared with the rifampin group (1.08 ±â€Š0.07) (P > 0.05). CONCLUSION: This study, despite its limitations, showed that rifampin with autogenous bone increased bone regeneration in rats with critical-size defects.

The influence of oral administration of rosuvastatin on calvarial bone healing in rats.

The purpose of this study is to investigate the potential of the systemic administration of different doses of rosuvastatin (RSV) on autogenous grafted critical-sized cortical bone defects. Twenty-four rats were divided into three groups: Group C (control), Group RSV-2 and Group RSV-5. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by autogenous graft only and rats were given saline solution with oral gavage for 28 days. In Group RSV-2 defects were filled with autogenous graft and rats were given 2 mg/kg rosuvastatin with oral gavage for 28 days. In Group RSV-5 defects were filled with autogenous graft and rats were given 5 mg/kg rosuvastatin with oral gavage for 28 days. All animals were euthanized at 28 days postoperative. Stereologic and micro-CT analyses were performed. New bone area (NBA) and connective tissue volumes were measured. Stereologic analysis showed that Group RSV-5 and RSV-2 had significantly more new bone at 4 weeks compared with group C. Connective tissue volumes were also significantly higher in RSV applicated groups. New bone and connective tissue volumes' difference were not statistically significant between RSV groups. Micro-CT results were similar with stereologic analyses. Orally administered RSV enhances bone regeneration in critical size calvarial rat defects filled with autogenous graft furthermore possible inflammatory effect should be investigated.

The effects of zoledronic acid on mandibular fracture healing in an osteoporotic model: a stereological study.

The aim of this study was to evaluate the effect of zoledronic acid (ZA) in the ovariectomized rat on mandibular fracture. Corpus osteotomies were performed on the mandible of ovariectomized rats. Miniplates were placed on these rats, which were randomly divided into 4 groups. After 7 days, groups 3 and 4 were injected with 0.1 mg/kg ZA. Groups 1 and 2 were control groups, so they were injected 0.1 mg/kg saline solution. Animals in groups 1 and 3 were killed at 4 weeks, whereas groups 2 and 4 were killed 8 weeks after operation. The new bone volume of the fracture gap was examined stereologically. Although the difference was not statistically significant compared with the other groups (groups 1 and 3), more bone formation was found in ZA-treated groups (groups 2 and 4) (P < 0.05).

Intraosseous carcinoma arising from an odontogenic cyst: a case report.

Carcinomas from an odontogenic cyst are not common, yet when they occur, squamous cell carcinomas are the most often seen. Among these cysts, the malignancy of keratocysts or dentigerous cysts are most likely. In contrast, a malignant transformation of a radicular cyst to an intraosseous carcinoma is extremely rare. In this case report, an intraosseous carcinoma arising from an odontogenic cyst in a 26-year-old male patient is presented. This case report clearly demonstrates the importance of the clinician's awareness of the malignant potential of apparently innocuous cystic lesions.