RESUMEN
Developmental delay is a deviation from the regular development of normative milestones during childhood. Early stimulation is a standardized and straightforward technique to support children with developmental delays (aged 0-3 years) in reaching basic motor skills, which are essential for the execution of everyday activities, such as playing, feeding and locomotion. In doing so, early stimulation reduces the chances of permanent motor impairment, thus allowing the child to live a more functional life. However, outcomes of this treatment depend heavily on the involvement of the family, who are required to continue the early stimulation activities at home on a daily basis. To empower and educate families to administer standardized early stimulation programs at home, we developed an electronic early stimulation program, which provides personalized guidance to parents to administer early stimulation; together with evidence-based clinical decision support to therapists in tailoring ESP to observed needs.
Asunto(s)
Discapacidades del Desarrollo , Padres , Técnicos Medios en Salud , Preescolar , Humanos , Lactante , Recién NacidoRESUMEN
Solvent-assisted protein digestion involves enzymatic hydrolysis in mixed aqueous-organic solvents. With trypsin, acetonitrile is the modifying solvent of choice, recommended at concentrations from 10 to 80% to improve protein sequence coverage in mass spectrometry. Spectroscopic activity assays employing substrate mimics such as N-benzoyl arginine ethyl ester (BAEE) appear to show a relative enhancement of trypsin activity in mixed solvent systems. However, as reported here, the changing solvent polarity induces bias in the absorbance measurement, lending upward of 35% error in the apparent trypsin activity as the acetonitrile is raised to 70%. Furthermore, time-dependent spectroscopic and mass spectrometric measurements reveal a progressive deactivation of trypsin over a 5- to 10-min period in as little as 30% acetonitrile.
