RESUMEN
OBJECTIVE: This study aimed to examine the association between lifestyle-related disorders and visceral fat mass, and to estimate an appropriate cutoff value for visceral fat mass that correlated with body mass index (BMI) and waist circumference (WC). METHODS: This cross-sectional study was conducted between July 2012 and August 2013 at Bange Kosei General Hospital, in Fukushima, Japan. All study participants were adult males who had completed voluntary medical check-ups that included estimation of visceral fat mass by bioelectrical impedance analysis (BIA). Participants were without past histories of atherosclerotic complications or were not currently taking medications for lifestyle-related disorders. Multivariate analysis was performed to estimate the association between lifestyle-related disorders and quartiles of visceral fat mass. RESULTS: Of 536 total respondents, 442 were included in the analysis. Mean participant age was 56 years, and mean values of BMI, WC, and visceral fat mass were 24.1 kg/m(2), 85.9 cm, and 2.1 kg, respectively. Visceral fat mass ≥1.8 kg was positively associated with an increased prevalence of dyslipidemia, elevated blood pressure, and impaired glucose tolerance. Cutoff values that correlated with visceral fat mass (≥1.8 kg) were 85.3 cm for WC and 23.25 kg/m(2) for BMI. CONCLUSION: Visceral fat mass ≥1.8 kg was positively associated with lifestyle-related disorders and closely related to WC and BMI cutoff values used to diagnose obesity. BIA may be a useful method for assessing visceral fat mass, and these findings provide important evidence for the use of BIA in the early detection of central obesity for preventing lifestyle-related disorders.
Asunto(s)
Dislipidemias/etiología , Intolerancia a la Glucosa/etiología , Hipertensión/etiología , Grasa Intraabdominal , Obesidad Abdominal/complicaciones , Adulto , Índice de Masa Corporal , Estudios Transversales , Dislipidemias/epidemiología , Impedancia Eléctrica , Intolerancia a la Glucosa/epidemiología , Humanos , Hipertensión/epidemiología , Japón/epidemiología , Estilo de Vida , Masculino , Persona de Mediana Edad , Obesidad Abdominal/epidemiología , Prevalencia , Factores de Riesgo , Circunferencia de la CinturaRESUMEN
DNA mismatch repair genes, hMLH1 and hMSH2, assigned on chromosome 3p21-23 and 2p21-22 are involved in hereditary non-polyposis colorectal cancer (HNPCC). The heterozygous carrier of the mutated allele results in a mutator phenotype and accelerating tumorigenesis, which especially causes carcinomas in the gastrointestinal and genitourinary tracts. We screened germline mutations of mismatch repair genes hMLH1 and hMSH2 in a patient with multiple primary neoplasms (multiple stomach cancers, colon cancer and brain tumor) in a cancer clustered HNPCC family. Screening by long RT-PCR from the RNA extracted from puromycin-treated heparinized blood showed skipping of the exon 2 in hMLH1. The analysis of the genomic DNA showed a GT deletion in the splice-donor site of the exon 2, which is compatible with the splicing variant detected by long RT-PCR analysis. This is a novel germline mutation that has not been reported previously.
Asunto(s)
Neoplasias Colorrectales Hereditarias sin Poliposis/genética , Mutación de Línea Germinal , Proteínas de Neoplasias/genética , Neoplasias Primarias Múltiples/genética , Proteínas Adaptadoras Transductoras de Señales , Adulto , Secuencia de Bases , Neoplasias Encefálicas/genética , Proteínas Portadoras , Neoplasias del Colon/genética , Femenino , Heterocigoto , Humanos , Datos de Secuencia Molecular , Homólogo 1 de la Proteína MutL , Proteínas Nucleares , Linaje , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Neoplasias Gástricas/genéticaRESUMEN
BACKGROUND: Despite curative resection of the primary tumor with extensive dissection of lymph nodes, some patients with node-negative gastric cancer die of local relapse or metastatic disease. Microinvolvement of regional lymph nodes may play an important role in the prognosis.METHODS: To evaluate the incidence and prognostic implications of regional lymph node microinvolvement in node-negative gastric cancer, we retrospectively analyzed tissue samples from 51 patients operated on for primary gastric cancer. They had tumors that invaded beyond the muscularis propria, but without metastasis to the lymph nodes, shown by the conventional H&E staining method. The regional lymph nodes were examined immunohistochemically, using monoclonal antibodies against cytokeratin.RESULTS: Microinvolvement was found in 4.8% of lymph nodes (67/1390) and in 43.2% of the patients (22/51). The clinical outcome of the patients with microinvolvement was not significantly different from those without it. However, no patient died in the no-microinvolvement group, while three patients in the microinvolvement group died of recurrence.CONCLUSION: The incidence of microinvolvement in conventionally negative lymph nodes cannot be ignored, and detecting microinvolvement may be important for predicting recurrence of gastric cancer.
