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BACKGROUND: Comparisons of traditional hunter-gatherers and pre-agricultural communities in Africa with urban and suburban Western North American and European cohorts have clearly shown that diet, lifestyle and environment are associated with gut microbiome composition. Yet, little is known about the gut microbiome composition of most communities in the very diverse African continent. South Africa comprises a richly diverse ethnolinguistic population that is experiencing an ongoing epidemiological transition and concurrent spike in the prevalence of obesity, largely attributed to a shift towards more Westernized diets and increasingly inactive lifestyle practices. To characterize the microbiome of African adults living in more mainstream lifestyle settings and investigate associations between the microbiome and obesity, we conducted a pilot study, designed collaboratively with community leaders, in two South African cohorts representative of urban and transitioning rural populations. As the rate of overweight and obesity is particularly high in women, we collected single time-point stool samples from 170 HIV-negative women (51 at Soweto; 119 at Bushbuckridge), performed 16S rRNA gene sequencing on these samples and compared the data to concurrently collected anthropometric data. RESULTS: We found the overall gut microbiome of our cohorts to be reflective of their ongoing epidemiological transition. Specifically, we find that geographical location was more important for sample clustering than lean/obese status and observed a relatively higher abundance of the Melainabacteria, Vampirovibrio, a predatory bacterium, in Bushbuckridge. Also, Prevotella, despite its generally high prevalence in the cohorts, showed an association with obesity. In comparisons with benchmarked datasets representative of non-Western populations, relatively higher abundance values were observed in our dataset for Barnesiella (log2fold change (FC) = 4.5), Alistipes (log2FC = 3.9), Bacteroides (log2FC = 4.2), Parabacteroides (log2FC = 3.1) and Treponema (log2FC = 1.6), with the exception of Prevotella (log2FC = - 4.7). CONCLUSIONS: Altogether, this work identifies putative microbial features associated with host health in a historically understudied community undergoing an epidemiological transition. Furthermore, we note the crucial role of community engagement to the success of a study in an African setting, the importance of more population-specific studies to inform targeted interventions as well as present a basic foundation for future research.
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Microbioma Gastrointestinal/genética , Estilo de Vida/etnología , Microbiota/genética , Adulto , Anciano , Bacterias/genética , Biomarcadores , Estudios de Cohortes , Dieta , Heces/microbiología , Femenino , Humanos , Persona de Mediana Edad , Obesidad/microbiología , Proyectos Piloto , ARN Ribosómico 16S/genética , Población Rural , Sudáfrica/etnologíaRESUMEN
We propose a method to manipulate the intensity and phase distributions of a beam with non-zero orbital angular momentum (OAM). We investigate the superposition of coherent consecutive OAM modes, with concordant topological charges values, showing that it is possible to predict and control the phase and the radial and angular dimension of the resulting beam by acting on the number of superposed modes (N) and on their minimum value of the OAM (m(min)). A general analysis from the Wigner function formalism is adopted for the geometric characterization of the beam.
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We introduce a new optical vortex coronagraph (OVC) method to determine the angular distance between two sources when the separation is sub-Rayleigh. We have found a direct relationship between the position of the minima and the source angular separation. A priori knowledge about the location of the two sources is not required. The superresolution capabilities of an OVC, equipped with an â = 2 N-step spiral phase plate in its optical path, were investigated numerically. The results of these investigations show that a fraction of the light, increasing with N, from the secondary source can be detected with a sub-Rayleigh resolution of at least 0.1 λ/D.
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Interpretación de Imagen Asistida por Computador/métodos , Modelos Teóricos , Telescopios , Simulación por Computador , Luz , Dispersión de RadiaciónRESUMEN
In the last years, advances in diagnosis and new treatments of cancer patient have increased the life expectancy of children, adolescent and women with cancer. Unfortunately, the ovaries are very sensitive to chemio-radiotherapy that may induce the loss of ovarian function and fertility with consequent premature ovarian failure. The different cryopreservation options available for fertility preservation in cancer patients are embryo cryopreservation, oocyte cryopreservation and ovarian tissue cryopreservation. The choice depends on different parameters: the type and timing of cancer treatment, the type of disease, the patient's age. The advances in reproductive technology have made fertility preservation a real possibility for patients whether they are girls or young women whose gonadal function is threatened by natural premature menopause, or by cancer therapy or surgical sterilisation.
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Criopreservación , Fertilidad , Feto , Oocitos , Ovario , Femenino , Humanos , Neoplasias Ováricas/terapiaRESUMEN
We experimentally and numerically tested the separability of two independent equally luminous monochromatic and white light sources at the diffraction limit, using optical vortices (OV). The diffraction pattern of one of the two sources crosses a fork hologram on its center generating the Laguerre-Gaussian (LG) transform of an Airy disk. The second source, crossing the fork hologram in positions different from the optical center, generates nonsymmetric LG patterns. We formulated a criterion, based on the asymmetric intensity distribution of the superposed LG patterns so created, to resolve the two sources at angular distances much below the Rayleigh criterion. Analogous experiments in white light allow angular resolutions which are still one order of magnitude below the Rayleigh criterion. The use of OVs might offer new applications for stellar separation in future space experiments.