ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens.

PURPOSE: The present study compared the expression of activity-regulated cytoskeleton-associated protein (ARC/Arg3.1) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of ARC/Arg3.1 in the lateral geniculate body in the pathogenesis of amblyopia. METHODS: Twenty kittens were randomly divided into an experimental group (n = 10) and a control group (n = 10). Black opaque covering cloth was used to cover the right eye of kittens in the experimental group. Pattern visual evoked potentials (PVEP) were detected weekly in all kittens. The expression of the ARC/Arg3.1 gene was detected by immunohistochemistry and in situ hybridization, and apoptosis of lateral geniculate body cells was detected by TUNEL. RESULTS: PVEP detection showed that at the age of 5 and 7 weeks, the latency of P100 in the right eye of the experimental group was higher than that of the other three groups (P < 0.05), and the amplitude of P100 was lower than that of the other three groups (P < 0.05). The expression of ARC/Arg3.1 protein (P < 0.05) and mRNA (P < 0.05) in the lateral geniculate body of the experimental group was significantly lower than that of the control group. The level of neuronal apoptosis in the experimental group was higher than that in the control group (P < 0.05). The expression of the ARC/Arg3.1 gene was negatively correlated with the apoptosis level of lateral geniculate body neurons. CONCLUSIONS: The expression of ARC/Arg3.1 is associated with monocular form deprivation amblyopia and apoptosis of lateral geniculate body cells.

Flammulina velutipes-derived carbon dots for fluorescence detection and imaging of hydroxyl radical.

Monitoring hydroxyl radical (•OH) fluctuation is of great importance to study some relative pathological processes and to predict early diagnosis of diseases. Efficient •OH-responsive fluorescent sensors based on carbon dots (CDs) have been reported, but most researches have focused on the new strategies for the synthesis and doping of the CDs. Herein, a kind of biomass CDs (F-CDs) with Flammulina velutipes (F. velutipes) as the carbon source was prepared by a one-step hydrothermal method without any additional modification. The prepared F-CDs have remarkable sensitivity and selectivity and there is a good linear relationship from 0 to 12 µM with a low detection limit of 95 nM for quantitative •OH assay. With excitation-independent emission, favourable biocompatibility and low toxicity, the F-CDs can penetrate cell membranes as •OH-responsive fluorescent sensors to detect intracellular •OH in A549 cells stimulated by phorbol 12-myristate 13-acetate (PMA) and successfully monitor the •OH concentration levels by the corresponding fluorescence change. Given the combined benefits of the green and eco-friendly approach, the F-CDs show promise as novel theranostics tools for early detection and treatment of related diseases.

Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens.

PURPOSE: The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia. METHODS: A total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization. RESULTS: PVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P < 0.05) and the right eye of control group (P < 0.05), while the amplitude decreased (P < 0.05). The number of positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in visual cortex of 8-week-old deprivation group were lower than those of normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05). CONCLUSIONS: Monocular form deprivation amblyopia can lead to the decrease of Egr-1 protein and mRNA expression in visual cortex, and then promote the occurrence and development of amblyopia.

Expression of brain-derived neurotrophic factor in the lateral geniculate body of monocular form deprivation amblyopic kittens.

PURPOSE: The present study compared the expression of brain-derived neurotrophic factor (BDNF) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of BDNF in the lateral geniculate body in the pathogenesis of amblyopia. METHODS: Twenty kittens were divided into control group (n = 10) and deprivation group (n = 10). A black opaque eye mask was placed to cover the right eye of the deprivation group. Pattern visual-evoked potentials (PVEPs) were detected weekly in all kittens .After the kittens in the deprivation group developed monocular amblyopia, the lateral geniculate bodies of all kittens were removed. The expression of BDNF in the lateral geniculate body of the two groups was compared by immunohistochemistry and Western blotting. RESULTS: The latency of the P100 wave in the right eye of the deprivation group was longer than that of the left eye and that of the right eye of the control group (p < 0.05), and the amplitude decreased (p < 0.05). The number and average optical density of BDNF-positive cells in the deprivation group were lower than those in the control group (p < 0.05), and the expression of BDNF in the deprivation group was lower than that in the control group (p < 0.05). CONCLUSIONS: The expression of BDNF in the lateral geniculate body of the amblyopic kittens decreased, and the decrease in BDNF promoted the development of amblyopia. These results demonstrate that BDNF in the lateral geniculate body plays an important role in visual development.

Dual-responsive mesoporous silica nanoparticles coated with carbon dots and polymers for drug encapsulation and delivery.

Aim: Smart mesoporous silica nanoparticles (MSNs) coated with carbon dots (CDs) and poly(N-vinylcaprolactam) (PNVCL) as a mixed shell (CDs/PNVCL polymer grafted MSNs) were prepared for pH-trigged anticancer drug release and real-time monitoring. Materials & methods: The amino-terminated PNVCL and amino-rich CDs were grafted onto the surface of aldehyde group functionalized MSNs through Schiff base reaction. Doxorubicin (DOX) was loaded into the prepared nanoparticles. Results: DOX could be quickly released in the tumor environment, leading to cell apoptosis. The linear fit between the percentage of released DOX and the fluorescence intensity of CDs indicated that the change in fluorescence intensity could be used to monitor drug release in real time. Conclusion: The as-prepared CDs/PNVCL polymer grafted MSNs are promising candidates for integrating controllable release and real-time monitoring in cancer treatment.

Mixed shell mesoporous silica nanoparticles for controlled drug encapsulation and delivery.

AIM: Smart mesoporous silica nanoparticles (MSNs) with mixed polymeric shell (MS-MSNs) were prepared to realize controlled encapsulation and responsive delivery of anticancer drugs. MATERIALS & METHODS: Two kinds of polymers, including nonthermoresponsive poly(ethylene glycol) and thermoresponsive poly(N-isopropyl acrylamide), were grafted onto the outlets of the MSNs through acidic liable Schiff base bonds. RESULTS: Poly(N-isopropyl acrylamide) chains could control the release rate of drugs through phase transition, while poly(ethylene glycol) chains could maintain the colloid stability of MSNs. Drugs can be released through the gradual hydrolysis of Schiff base bonds in tumor acidic environment. CONCLUSION: The MS-MSNs gave consideration to both the responsiveness and stability of carriers, and could realize the release of drugs as much as possible in tumor tissues.

Nucleobase chemosensor based on carbon nanodots.

A facile and sensitive fluorescence protocol for nucleobase detection was developed based on carbon nanodot (CD) chemosensors. The novel fluorescent CDs were prepared using four kinds of nucleobases (including adenine, guanine, thymine and cytosine) as separate carbon sources via simple hydrothermal strategy. The quantum yield of adenine CDs (A-CDs), guanine CDs (G-CDs), thymine CDs (T-CDs) and cytosine CDs (C-CDs) was checked as 15.1%, 28.3%, 10.6% and 11.7%, respectively. Four CDs can recognize their complementary nucleobases based on the principle of complementary base pairing. Their fluorescence was linearly quenched with the increase of nucleobase concentrations under optimal conditions. Combining the calibration curve, quantitative assay of nucleobase in solution can be realized. For example, A-CDs could determine thymine in the concentration range of 2-20mM with a detection limit of ca. 0.053mM, and the linear equation is fitting as (I0-I) / I = 0.01961 × CT(mM) + 0.01756 (R2 = 0.994). Thymine can induce the fluorescence lifetime of A-CDs decreasing from 5.58 to 3.34ns, indicating a dynamic quenching mechanism. The novel nucleobase sensors were also evaluated in specific solution environment. A-CDs showed a relatively minor relative standard deviation (< 4.0%) in fetal calf serum solution, indicating a high accuracy and credibility of the sensing system. In view of the excellent sensitivity, preferable biocompatibility as well as simple constructing method, the sensing platform derived from the nucleobase-based CDs present great potential in biological sensing applications.

Thermo/pH Dual Responsive Mixed-Shell Polymeric Micelles Based on the Complementary Multiple Hydrogen Bonds for Drug Delivery.

Thermo/pH dual responsive mixed-shell polymeric micelles based on multiple hydrogen bonding were prepared by self-assembly of diaminotriazine-terminated poly(ɛ-caprolactone) (DAT-PCL), uracil-terminated methoxy poly(ethylene glycol) (MPEG-U), and uracil-terminated poly(N-vinylcaprolactam) (PNVCL-U) at room temperature. PCL acted as the core and MPEG/PNVCL as the mixed shell. Increasing the temperature, PNVCL collapsed and enclosed the PCL core, while MPEG penetrated through the PNVCL shell, thereby leading to the formation of MPEG channels on the micelles surface. The low cytotoxicity of the mixed micelles was confirmed by an MTT assay against BGC-823 cells. Studies on the in vitro drug release showed that a much faster release rate was observed at pH 5.0 compared to physiological pH, owing to the dissociation of hydrogen bonds. Therefore, the mixed-shell polymeric micelles would be very promising candidates in drug delivery systems.

Synthesis and self-assembly of thermoresponsive amphiphilic biodegradable polypeptide/poly(ethyl ethylene phosphate) block copolymers.

We report the design and synthesis of new fully biodegradable thermoresponsive amphiphilic poly(γ-benzyl L-glutamate)/poly(ethyl ethylene phosphate) (PBLG-b-PEEP) block copolymers by ring-opening polymerization of N-carboxy-γ-benzyl L-glutamate anhydride (BLG-NCA) with amine-terminated poly(ethyl ethylene phosphate) (H2N-PEEP) as a macroinitiator. The fluorescence technique demonstrated that the block copolymers could form micelles composed of a hydrophobic core and a hydrophilic shell in aqueous solution. The morphology of the micelles as determined by transmission electron microscopy (TEM) was spherical. The size and critical micelle concentration (CMC) values of the micelles showed a decreasing trend as the PBLG segment increased. However, UV/Vis measurements showed that these block copolymers exhibited a reproducible temperature-responsive behavior with a lower critical solution temperature (LCST) that could be tuned by the block composition and the concentration.

[Relationship between the oral language development and restoration of velopharyngeal closure function in Chinese toddlers with repaired cleft palate].

OBJECTIVE: To evaluate how the oral language development enhances articulators to function well in cleft palate movement and velopharyngeal closure in the young Chinese children with repaired cleft palate. METHODS: The recordings of 78 cases of Chinese toddlers with repaired cleft palate were reviewed. This group of children aged from 27 months to 33 months (average: 30 months). Transcription using Pinyin system was made. Mean utterance count (MUC) and mean special consonant correct count (MSCC) were calculated. Correlation between MUC and MSCC was statistically analyzed. RESULTS: The correlation coefficient of MUC and MSCC is 0.360 (P < 0.01). CONCLUSIONS: There is positive correlation between the oral language development and restoration of velopharyngeal closure function. Children with repaired cleft palate should be encouraged to start oral language as early as possible and as much as possible in order to get the restoration of velopharyngeal closure function.