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This study investigated the ability of the near-infrared spectroscopy (NIRS) model to predict the protein of freeze-dried muscle samples in Chinese native chickens and to determine the accuracy of the models for other native chicken breeds. Spectral pretreatment, wavelength selection, and outlier sample elimination were used to optimize the calibration models. The results showed that the best model was obtained by using a combination of standard normal variable transformation and gap-segment first-derivative pretreatment spectra after removing 48 outliers in the wavelength range of 1,439 to 1,900 nm, with coefficient of determination for the calibration (R2C) of 0.95, standard error of cross-validation (SECV) of 1.18, coefficient of determination for the prediction (R2P) of 0.95, the ratio of the standard deviation of the validation to the standard deviation of the calibration (RPDP) of 4.62. The findings indicated that NIRS can be used to predict the protein of freeze-dried muscle in Chinese native chickens.
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Pollos , Espectroscopía Infrarroja Corta , Animales , Espectroscopía Infrarroja Corta/veterinaria , Espectroscopía Infrarroja Corta/métodos , Proteínas Musculares , Proteínas de Plantas , Calibración , Análisis de los Mínimos CuadradosRESUMEN
This study investigates the effect of grinding methods, including roller mill, hammer mill, and two-stage grinding, on the particle size distribution of corn and the effect of corn particle size on the nutrient digestibility of native chickens in Southwest China. The roller mill, hammer mill, and a combination of the hammer mill and roller mill were used to obtain corn with various coarseness. Corn with different coarseness obtained using a combination of the hammer mill and roller mill was fed to Chahua chicken No. 2-type chickens (CHC2s). A total of 192 CHC2s in weeks 12 and 19 were randomly allocated to eight groups in triplicate. The results show that the geometric mean diameter (dgw) and the geometric standard deviation (Sgw) were significantly (p < 0.05) affected by the grinding methods. The Sgw obtained when using a sieve of 2.0 mm in a hammer mill was lower (p < 0.05) than that obtained using a 4.5 mm sieve. Combining the roller mill and hammer mill increased the uniformity of the particle size when grinding coarse particles. For fine particles, the dgw and Sgw obtained when using the hammer mill were significantly lower (p < 0.05) than those obtained when using the roller mill and two-stage grinding method. Reducing the particle size of the corn (<900 µm) significantly increased the dry matter, crude protein, amino acid digestibility, and apparent metabolizable energy in the chicken in weeks 12 and 19. Fine particles significantly increased the crude protein digestibility of the CHC2s at week 12, while there was no significant effect on the crude protein and amino acid digestibility in the CHC2s at week 19. In conclusion, different grinding methods can affect the particle size distribution. For a coarse particle size, combining the roller mill and hammer mill tends to produce a more uniform particle size. Finely ground corn (between 700 µm and 900 µm) improved the dry matter (DM), apparent metabolizable energy (AME), and crude protein (CP) digestibility of the CHC2s at week 12. An increased particle size did not impact the CP and amino acid (AA) digestibility of the CHC2s at week 19.
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BACKGROUND: Intensive selection has resulted in increased growth rates and muscularity in broiler chickens, in addition to adverse effects, including delayed organ development, sudden death syndrome, and altered metabolic rates. The biological mechanisms underlying selection responses remain largely unknown. Non-artificially-selected indigenous Chinese chicken breeds display a wide variety of phenotypes, including differential growth rate, body weight, and muscularity. The Wuding chicken breed is a fast growing large chicken breed, and the Daweishan mini chicken breed is a slow growing small chicken breed. Together they form an ideal model system to study the biological mechanisms underlying broiler chicken selection responses in a natural system. The objective of this study was to study the biological mechanisms underlying differential phenotypes between the two breeds in muscle and liver tissues, and relate these to the growth rate and body development phenotypes of the two breeds. RESULTS: The muscle tissue in the Wuding breed showed higher expression of muscle development genes than muscle tissue in the Daweishan chicken breed. This expression was accompanied by higher expression of acute inflammatory response genes in Wuding chicken than in Daweishan chicken. The muscle tissue of the Daweishan mini chicken breed showed higher expression of genes involved in several metabolic mechanisms including endoplasmic reticulum, protein and lipid metabolism, energy metabolism, as well as specific immune traits than in the Wuding chicken. The liver tissue showed fewer differences between the two breeds. Genes displaying higher expression in the Wuding breed than in the Daweishan breed were not associated with a specific gene network or biological mechanism. Genes highly expressed in the Daweishan mini chicken breed compared to the Wuding breed were enriched for protein metabolism, ABC receptors, signal transduction, and IL6-related mechanisms. CONCLUSIONS: We conclude that faster growth rates and larger body size are related to increased expression of genes involved in muscle development and immune response in muscle, while slower growth rates and smaller body size are related to increased general cellular metabolism. The liver of the Daweishan breed displayed increased expression of metabolic genes.
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Peso Corporal , Pollos/crecimiento & desarrollo , Fenotipo , Animales , Composición Corporal , Pollos/genética , Perfilación de la Expresión Génica , Hígado/metabolismo , Desarrollo de Músculos , Músculos/metabolismo , Especificidad de la EspecieRESUMEN
The objective of the research was to study the correlations between near infrared spectra and molecular structures of 20 standard amino acids. It was to establish the theoretical foundation for widely use of the amino acids near infrared spectra in animal science, food and medicine. Measurement of the near infrared spectra was performed using a Shimadzu Fourier transform infrared spectrophotometer IRPrestige-21, with FlexIRTM Near-Infrared Fiber Optics module. The spectrometric data acquisitions were performed by Shimadzu IRsolution 1.50 system. The spectrometric room temperature was 25 â and humidity was 38%. Spectra of 20 amino acid standard substances were collected by reflectance mode from 1 000 to 2 502 nm in 8 cm-1 increment. Each sample was scanned in three times, each scan was 50 cycles, and the average value of three times scan result was used for each sample. Based on the differences of amino acids side chains, the correlations between near infrared spectra and molecular structures were compared in the fat family amino acids, aromatic amino acids and heterocycle amino acids. The result shows that all 20 standard amino acids have very specific absorption line patterns. It is distinctly different in these absorption line patterns. Near-infrared spectra of high molecular weight fat family amino acids are affected by side chains. Near-infrared spectra of glycine are affected by carboxyl and amino. The differences of near-infrared spectra between two aromatic amino acids are in benzene ring. OH groups on benzene ring of tyrosine lower the symmetry of benzene molecule. It leads to the emergence of more vibration absorption. Near-infrared spectra of heterocycle amino acids are distinctly different in 1 000ï½2 502 nm because of side chains. In conclusion, there are four different characteristic spectral regions. The first one is 1 050ï½1 200 nm spectral region which is composed mainly of second-order frequency doubling of CH group. The second is 1 300ï½1 500 nm spectral region which is composed mainly of combination tune of CH group. Due to side chains of amino acid have different molecular structure, they yield a complete set of near infrared fingerprint spectra between 1 600ï½1 850 and 2 000ï½2 502 nm. In another words, these four characteristic regions of near infrared spectra can be used to build the model of qualitative analysis and quantitative analysis for amino acid, and improves the accuracy and reliability of model.
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This study was conducted to evaluate the effects of cholesterol on growth and cholesterol metabolism of rainbow trout (Oncorhynchus mykiss) fed diets with cottonseed meal (CSM) or rapeseed meal (RSM). Four experimental diets were formulated to contain 550 g kg(-1) CSM or 450 g kg(-1) RSM with or without 9 g kg(-1) supplemental cholesterol. Growth rate and feed utilization efficiency of fish fed diets with 450 g kg(-1) RSM were inferior to fish fed diets with 550 g kg(-1) CSM regardless of cholesterol level. Dietary cholesterol supplementation increased the growth rate of fish fed diets with RSM, and growth rate and feed utilization efficiency of fish fed diets with CSM. Similarly, dietary cholesterol supplementation increased the plasma total cholesterol (TC), high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triiodothyronine levels, but decreased the plasma triglycerides and cortisol levels of fish fed diets with RSM or CSM. In addition, supplemental cholesterol increased the free cholesterol and TC levels in intestinal contents, but decreased the hepatic 3-hydroxy-3-methyl-glutaryl-CoA reductase activity of fish fed diets with RSM or CSM. These results indicate that 9 g kg(-1) cholesterol supplementation seems to improve the growth of rainbow trout fed diets with CSM or RSM, and the growth-promoting action may be related to the alleviation of the negative effects caused by antinutritional factors and/or make up for the deficiency of endogenous cholesterol in rainbow trout.
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Alimentación Animal/análisis , Brassica rapa/química , Aceite de Semillas de Algodón/química , Dieta/veterinaria , Oncorhynchus mykiss/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Calcio/sangre , Colesterol/sangre , Colesterol/metabolismo , Colesterol/farmacología , Colesterol 7-alfa-Hidroxilasa/genética , Colesterol 7-alfa-Hidroxilasa/metabolismo , Colesterol en la Dieta/metabolismo , Hidrocortisona/sangre , Mucosa Intestinal/metabolismo , Hígado/enzimología , Hígado/metabolismo , Músculo Esquelético/metabolismo , Oncorhynchus mykiss/sangre , Fósforo/metabolismo , Tiroxina/sangre , Triyodotironina/sangreRESUMEN
The physiological mechanisms of thermogenesis, energy balance and energy expenditure are poorly understood in poultry. The aim of this study was designed to investigate the physiological roles of avian uncoupling protein (avUCP) regulating in energy balance and thermogenesis by using three chicken breeds of existence striking genetic difference and feeding with different dietary protein levels. Three chicken breeds including broilers, hybrid chickens, and non-selection Wuding chickens were used in this study. Total 150 chicks of 1 day of age, with 50 from each breed were reared under standard conditions on starter diets to 30 days. At 30 days of age, forty chicks from each breed chicks were divided into two groups. One group was fed low protein diet (LP, 17.0 %), and the other group was fed high protein diet (HP, 19.5 %) for 60 days. Wuding chickens showed the lowest feed conversion efficiency (FCE) and the highest expressions of avUCP mRNA association with high plasma T3 and insulin concentrations. Hybrid chickens showed the lowest expressions of avUCP mRNA association with high FCE and energy efficiency. Expressions of avUCP mRNA association with diet-induced thermogenesis (DIT) were only observed in broiler and hybrid chickens. The expressions of avUCP mRNA were positive association with plasma insulin, T3 and NEFA concentrations. Age influence on the expression of avUCP mRNA were observed only for hybrid and broiler chickens. It seems that both roles of avUCP regulation thermogenesis and lipid utilisation as fuel were observed in the present study response to variation in dietary protein and breeds.
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Proteínas Aviares/genética , Proteínas en la Dieta/administración & dosificación , Metabolismo Energético/efectos de los fármacos , Proteínas Mitocondriales/genética , Termogénesis/genética , Animales , Cruzamiento , Pollos/genética , Pollos/fisiología , Metabolismo Energético/genética , Regulación de la Expresión Génica/efectos de los fármacos , Masculino , Proteínas Desacopladoras Mitocondriales , ARN Mensajero/genética , Termogénesis/efectos de los fármacosRESUMEN
In contrast to conventional methods for the determination of meat chemical composition, near infrared reflectance spectroscopy enables rapid, simple, secure and simultaneous assessment of numerous meat properties. The present review focuses on the use of near infrared reflectance spectroscopy to predict meat chemical compositions. The potential of near infrared reflectance spectroscopy to predict crude protein, intramuscular fat, fatty acid, moisture, ash, myoglobin and collagen of beef, pork, chicken and lamb is reviewed. This paper discusses existing questions and reasons in the current research. According to the published results, although published results vary considerably, they suggest that near-infrared reflectance spectroscopy shows a great potential to replace the expensive and time-consuming chemical analysis of meat composition. In particular, under commercial conditions where simultaneous measurements of different chemical components are required, near infrared reflectance spectroscopy is expected to be the method of choice. The majority of studies selected feature-related wavelengths using principal components regression, developed the calibration model using partial least squares and modified partial least squares, and estimated the prediction accuracy by means of cross-validation using the same sample set previously used for the calibration. Meat fatty acid composition predicted by near-infrared spectroscopy and non-destructive prediction and visualization of chemical composition in meat using near-infrared hyperspectral imaging and multivariate regression are the hot studying field now. On the other hand, near infrared reflectance spectroscopy shows great difference for predicting different attributes of meat quality which are closely related to the selection of calibration sample set, preprocessing of near-infrared spectroscopy and modeling approach. Sample preparation also has an important effect on the reliability of NIR prediction; in particular, lack of homogeneity of the meat samples influenced the accuracy of estimation of chemical components. In general the predicting results of intramuscular fat, fatty acid and moisture are best, the predicting results of crude protein and myoglobin are better, while the predicting results of ash and collagen are less accurate.
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Análisis de los Alimentos/métodos , Carne/análisis , Espectroscopía Infrarroja Corta , Animales , Calibración , Bovinos , Pollos , Ácidos Grasos , Análisis de los Mínimos Cuadrados , Modelos Teóricos , Proteínas , Reproducibilidad de los Resultados , Ovinos , PorcinosRESUMEN
This study evaluated the effects of dietary cholesterol on antioxidant capacity, non-specific immune response and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) fed soybean meal-based diets. Fish were fed diets supplemented with graded cholesterol levels (0 [control], 0.3, 0.6, 0.9, 1.2, and 1.5%) for nine weeks. The fish were then challenged by A. hydrophila and their survival rate recorded for the next week. Dietary cholesterol supplementation generally increased the serum and hepatic superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (TAC) activities, but decreased the serum and hepatic malondialdehyde (MDA) contents. Further, the hepatic CAT and serum SOD, CAT, and TAC activities were significantly higher in fish fed diets supplemented with 0.9 or 1.2% cholesterol compared to those fed the control diet, whereas the serum and hepatic MDA contents were significantly lower. The respiratory burst activity, alternative complement activity, and hepatic lysozyme activity increased steadily when the supplemental cholesterol was increased by up to 1.2% and then declined with further addition. The serum lysozyme activity and phagocytic activity increased steadily with increasing dietary supplemental cholesterol level up to 0.9% and then declined with further addition. Dietary cholesterol supplementation generally enhanced the protection against A. hydrophila infection, and fish fed diets supplemented with 0.9 or 1.2% cholesterol exhibited the highest post-challenge survival rate. The results indicated that cholesterol may be under-supplied in rainbow trout fed soybean meal-based diets, and dietary cholesterol supplementation (0.9-1.2%) contributed to improved immune response and disease resistance of rainbow trout against A. hydrophila.
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Acuicultura , Colesterol/administración & dosificación , Suplementos Dietéticos/análisis , Enfermedades de los Peces/inmunología , Infecciones por Bacterias Gramnegativas/veterinaria , Oncorhynchus mykiss/inmunología , Aeromonas hydrophila/inmunología , Alimentación Animal/análisis , Animales , Antioxidantes/metabolismo , Vía Alternativa del Complemento , Dieta/veterinaria , Resistencia a la Enfermedad/efectos de los fármacos , Enfermedades de los Peces/mortalidad , Enfermedades de los Peces/prevención & control , Proteínas de Peces/metabolismo , Infecciones por Bacterias Gramnegativas/inmunología , Infecciones por Bacterias Gramnegativas/mortalidad , Infecciones por Bacterias Gramnegativas/prevención & control , Inmunidad Innata/efectos de los fármacos , Muramidasa/metabolismo , Oncorhynchus mykiss/crecimiento & desarrollo , Fagocitosis/efectos de los fármacos , Estallido RespiratorioRESUMEN
This study was conducted to evaluate the effect of ethanolic extract of propolis (EEP) on growth performance and plasma biochemical parameters of rainbow trout (Oncorhynchus mykiss). Graded levels of EEP [0 (control), 1, 2, and 4 g kg(-1) diet] were fed to trout juveniles (mean weight 7.73 ± 0.17 g) for 10 weeks. Dietary EEP supplementation regardless of inclusion level significantly improved the specific growth rate of fish. Similarly, supplemental EEP generally improved the feed efficiency ratio and protein efficiency ratio, but no significant differences were observed between the 1 g kg(-1) EEP group and the control group. In addition, dietary EEP supplementation generally increased the plasma superoxide dismutase, lysozyme, total antioxidant capacity, glutathione peroxidase, and catalase activities, but decreased the plasma malondialdehyde level. The plasma triglycerides level was significantly lower in the 1 or 4 g kg(-1) EEP group as compared with the control group. Dietary EEP supplementation generally decreased the plasma aspartate aminotransferase and alanine aminotransferase activities, but increased the hepatic aspartate aminotransferase and alanine aminotransferase activities. These results indicate the potential to use the EEP as a growth promoter, hepatoprotective agent, and immunostimulant for rainbow trout.
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Factores Inmunológicos/farmacología , Hígado/efectos de los fármacos , Oncorhynchus mykiss/crecimiento & desarrollo , Própolis/farmacología , Animales , Acuicultura , Oncorhynchus mykiss/sangreRESUMEN
Uncoupling protein 1 (UCP1), a 32-kDa protein located in the inner mitochondrial membrane, is abundant in brown adipose tissue, as a proton transporter in mitochondria inner membrane which uncouples oxidative metabolism from ATP synthesis and dissipates energy through the heat. UCP1 has been reported to play important roles for energy homeostasis in rodents and neonate of larger mammals including human. Recently, numerous candidate genes were searched to determine the genetic factors implicated in the pathogenesis of obesity, related metabolic disorders and diabetes. UCP-1, which plays a major role in thermogenesis, was suggested to be one of the candidates. This review summarizes data supporting the existence of brown adipocytes and the role of UCP1 in energy dissipation in adult humans, and the genetic variety association with the fat metabolism, obesity and diabetes.