Silencing SIX1 by miR-7160 inhibits non-small cell lung cancer cell growth.

The homeoprotein SIX1 is upregulated in non-small cell lung cancer (NSCLC) and associated with NSCLC tumorigenesis and progression. We identified microRNA-7160 (miR-7160) as a SIX1-targeting miRNA. RNA immunoprecipitation results confirmed a direct binding between miR-7160 and SIX1 mRNA in NSCLC cells. In the primary and established NSCLC cells, forced overexpression of miR-7160 downregulated SIX1 and inhibited cancer cell growth, proliferation, migration and invasion. Furthermore, miR-7160 overexpression induced apoptosis activation in NSCLC cells. Conversely, miR-7160 inhibition elevated SIX1 expression and enhanced NSCLC cell progression in vitro. Restoring SIX1 expression, by an untranslated region-depleted SIX1 expression construct, reversed miR-7160-induced anti-NSCLC cell activity. CRISPR/Cas9-inudced knockout of SIX1 mimicked miR-7160-induced actions and produced anti-NSCLC cell activity. In vivo, intratumoral injection of miR-7160-expressing lentivirus downregulated SIX1 mRNA and inhibited NSCLC xenograft growth in severe combined immunodeficient mice. Significantly, miR-7160 expression is downregulated in human NSCLC tissues and is correlated with SIX1 mRNA upregulation. Collectively, miR-7160 silenced SIX1 and inhibited NSCLC cell growth in vitro and in vivo.

Novel single component tri-rare-earth emitting MOF for warm white light LEDs.

Single-phase white phosphors could overcome many drawbacks of traditional phosphors, and warm white light phosphors have been considered suitable for indoor illumination applications. Thus, synthesizing new single-phase warm white phosphors is significant for the production of LEDs. Based on the above considerations, a novel single component warm white light phosphor SmxTbyDy0.2-x-y-metal organic frameworks (MOFs) has been prepared successfully in this study. The compound Sm0.1Tb0.04Dy0.06-MOF shows ideal warm white light emission with CIE coordinates (0.333, 0.3522), a color rendering index (CRI) value of 86.7, a low correlated color temperature (CCT) value of 4444 K, and good heating/cooling circulation. In addition, the LED devices fabricated with this novel warm white light phosphor have excellent warm white light quality even at high temperatures, which is necessary for its practical application.

Effects of Zn²âº and Cu²âº on loach ovaries and ova development.

This study compared the accumulation of Zn²âº and Cu²âº in the ovaries and ova of loaches under different concentrations of Zn²âº (1.00, 2.50 and 5.00 mg/L respectively) and Cu²âº (0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn²âº and Cu²âº accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions (P<0.05), and was obviously affected by the time and doses. However, the Cu²âº concentration was significantly higher than Zn²âº (P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn²âº and Cu²âº solutions.This study compared the accumulation of Zn2+and Cu2+ in the ovaries and ova of loaches under different concentrations of Zn2+ (1.00, 2.50 and 5.00 mg/L respectively) and Cu2+ (0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+ and Cu2+ accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions (P<0.05), and was obviously affected by the time and doses. However, the Cu2+ concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+ and Cu2+ solutions.