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1.
Photodiagnosis Photodyn Ther ; 48: 104220, 2024 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-38777309

RESUMEN

BACKGROUND: Accurate diagnosis of patients with ulcerative colitis (UC) can reduce their risk of developing colorectal cancer. This study intended to explore whether moxifloxacin, an agent with fluorescence potential, could promote two-photon microscopy (TPM) diagnosis for mice with dextran sodium sulfate (DSS)-induced colitis, which could imitate human UC. METHODS: 32 Balb/c mice were randomly divided into 4 groups: control, acute colitis, remission colitis and chronic colitis. Fluorescence parameters, imaging performance, and tissue features of different mouse models were compared under moxifloxacin-assisted TPM and label-free TPM. RESULTS: Excitation wavelength of 720 nm and moxifloxacin labeling time of 2 min was optimal for moxifloxacin-assisted TPM. With moxifloxacin labeling for colonic tissues, excitation power was decreased to 1/10 of that without labeling while fluorescence intensity was increased to 10-fold of that without labeling. Photobleaching was negligible after moxifloxacin labeling and moxifloxacin fluorescence kept stable within 2 h. Compared with the control group, moxifloxacin fluorescence was reduced in the three colitis groups (P < 0.05). Meanwhile, the proportion of enhanced moxifloxacin fluorescence regions was (22.4 ± 1.6)%, (7.7 ± 1.0)%, (13.5 ± 1.7)% and (5.0 ± 1.3)% in the control, acute, remission and chronic groups respectively, with significant reduction in the three colitis groups (P < 0.05). Besides, variant tissue features of experimental colitis models were presented under moxifloxacin-assisted TPM, such as crypt opening, glandular structure, adjacent glandular space and moxifloxacin distribution. CONCLUSIONS: With unique biological interaction between moxifloxacin and colonic mucosa, moxifloxacin-assisted TPM imaging is feasible and effective for accurate diagnosis of different stages of experimental colitis.

2.
Cancer Gene Ther ; 31(6): 871-883, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38459370

RESUMEN

EGR4 (Early Growth Response 4) is a member of the EGR family, involving in tumorigenesis. However, the function and action mechanism of EGR4 in the pathogenesis of colorectal cancer (CRC) remain unclear. To address this, we assessed the prognosis of CRC based on EGR4 using the Kaplan-Meier plotter tool and tissue microarray. The abundance of immunoinfiltration was evaluated through ssGSEA, TISIDB, and TIMER. In vitro experiments involving knockdown or overexpression of EGR4 were performed, and RNA-sequencing was conducted to explore potential mechanisms. Furthermore, we used oxaliplatin and 5-fluorouracil to validate the impact of EGR4 on chemo-resistance. Pan-cancer analysis and tissue microarray showed that EGR4 was highly expressed in CRC and significantly correlated with an unfavorable prognosis. Moreover, EGR4 expression was associated with immunoinfiltration and cancer-associated fibroblasts in the CRC microenvironment. Functional enrichment demonstrated that high-expressional EGR4 were involved in chromatin and nucleosome assembly. Additionally, EGR4 promoted the proliferation of CRC cells. Mechanistically, EGR4 upregulated TNFα to activate the NF-κB signaling pathway, and its knockdown reduced p65 nuclear translocation. Importantly, combining shEGR4 with oxaliplatin and 5-fluorouracil significantly inhibited CRC proliferation. Taken together, these findings provide new insights into the potential prognosis and therapeutic targets of EGR4 in CRC.


Asunto(s)
Biomarcadores de Tumor , Proliferación Celular , Neoplasias Colorrectales , Humanos , Neoplasias Colorrectales/metabolismo , Neoplasias Colorrectales/genética , Neoplasias Colorrectales/patología , Neoplasias Colorrectales/tratamiento farmacológico , Biomarcadores de Tumor/metabolismo , Biomarcadores de Tumor/genética , Pronóstico , Ratones , Oxaliplatino/farmacología , Oxaliplatino/uso terapéutico , Fluorouracilo/farmacología , Fluorouracilo/uso terapéutico , Animales , Femenino , Regulación Neoplásica de la Expresión Génica , Resistencia a Antineoplásicos , Masculino , Línea Celular Tumoral , Estudios Prospectivos
3.
Sci Rep ; 14(1): 4018, 2024 02 18.
Artículo en Inglés | MEDLINE | ID: mdl-38369634

RESUMEN

The role of ATP6AP1 in colorectal cancer (CRC) remains elusive despite its observed upregulation in pan-cancer. Therefore, the current study aimed to assess the clinical significance of ATP6AP1 and its relationship with the immune infiltration in CRC. Transcriptome data of CRC were obtained from The Cancer Genome Atlas (TCGA) database and analyzed using the combination of R packages and tumor-related databases, including TIMER2, TISIDB, cBioPortal, and MethSurv. The tissue arrays and immunohistochemical staining were performed to verify the expression and clinical characteristics of ATP6AP1. The results revealed that ATP6AP1 expression was significantly elevated in CRC and associated with poor clinicopathological characteristics and prognosis. Furthermore, the analysis demonstrated ATP6AP1 expression was correlated with the infiltration of immune cells and cancer-associated fibroblasts in the microenvironment of CRC. Moreover, ATP6AP1 was found to be linked to various immune checkpoints and chemokines, with enrichment of cytoplasmic vesicle lumen, endopeptidase regulator activity, and endopeptidase inhibitor activity observed in the high ATP6AP1 expressional group. In conclusion, the findings of this study suggest that ATP6AP1 upregulation may serve as a biomarker for poor diagnosis in CRC and offer a potential target for immunotherapy in CRC.


Asunto(s)
Fibroblastos Asociados al Cáncer , Neoplasias Colorrectales , ATPasas de Translocación de Protón Vacuolares , Humanos , Neoplasias Colorrectales/genética , Vesículas Citoplasmáticas , Pronóstico , Microambiente Tumoral , ATPasas de Translocación de Protón Vacuolares/genética
4.
Eur J Med Res ; 29(1): 20, 2024 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-38178258

RESUMEN

BACKGROUND: SLC10A3, a gene upregulated in pan-cancer, lacks full understanding regarding its prognostic implications and association with immune infiltration in colorectal cancer (CRC). This study comprehensively analyzed SLC10A3 in CRC, evaluating its prognostic significance and influence on the tumor's immune microenvironment. METHODS: Transcriptomic data from TCGA were obtained to compare SLC10A3 expression in both colorectal cancer (CRC) and normal tissues. Prognostic value was assessed for overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI). DNA methylation patterns of SLC10A3 and correlation with DNA mismatch repair (MMR) were explored. Genetic alterations in SLC10A3 were scrutinized. The study also delved into the influence of SLC10A3 on the immune microenvironment of CRC, including immune cell infiltration and chemokines. Involvement of cancer-associated fibroblasts (CAFs) was explored. Methylation status of specific CpG islands in the SLC10A3 gene correlated with CRC patient prognosis. CRC tissue microarray was performed to verify the expression of SLC10A3 and its relationship with prognosis. RESULTS: The research revealed that SLC10A3 is significantly upregulated in CRC and holds promise as a potential diagnostic marker. Elevated SLC10A3 expression was linked to poorer OS, DSS, and PFI. Methylation patterns of SLC10A3 displayed prognostic relevance, and genetic alterations in the gene were identified. SLC10A3 was shown to impact the immune microenvironment, with significant correlations observed between its expression and various immune cell types, chemokines, and markers associated with CAFs. Furthermore, an inverse relationship between SLC10A3 and MMR molecules was established. Methylation status of specific CpG islands within the SLC10A3 gene was associated with CRC patient prognosis. Tissue microarray showed that SLC10A3 was highly expressed in CRC and significantly correlated with poor prognosis. CONCLUSION: The study underscores the importance of elevated SLC10A3 in CRC, associating it with decreased survival and immune infiltration, proposing it as a diagnostic biomarker and appealing immunotherapy target, given its significant overexpression and influence on the immune microenvironment and prognosis through methylation patterns.


Asunto(s)
Adenocarcinoma , Neoplasias Colorrectales , Humanos , Adenocarcinoma/genética , Neoplasias Colorrectales/genética , Pronóstico , Metilación de ADN/genética , Quimiocinas , Microambiente Tumoral/genética
6.
Comput Intell Neurosci ; 2022: 4926124, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35341171

RESUMEN

Deep learning-based image compression methods have made significant achievements recently, of which the two key components are the entropy model for latent representations and the encoder-decoder network. Both the inaccurate estimation of the entropy estimation model and the existence of information redundancy in latent representations lead to a reduction in the compression efficiency. To address these issues, the study suggests an image compression method based on a hybrid domain attention mechanism and postprocessing improvement. This study embeds hybrid domain attention modules as nonlinear transformers in both the main encoder-decoder network and the hyperprior network, aiming at constructing more compact latent features and hyperpriors and then model the latent features as parametric Gaussian-scale mixture models to obtain more precise entropy estimation. In addition, we propose a solution to the errors introduced by quantization in image compression by adding an inverse quantization module. On the decoding side, we also provide a postprocessing enhancement module to further increase image compression performance. The experimental results show that the peak signal-to-noise rate (PSNR) and multiscale structural similarity (MS-SSIM) of the proposed method are higher than those of traditional compression methods and advanced neural network-based methods.


Asunto(s)
Compresión de Datos , Suministros de Energía Eléctrica , Entropía , Redes Neurales de la Computación , Distribución Normal
7.
Rev Esp Enferm Dig ; 114(8): 503-504, 2022 08.
Artículo en Inglés | MEDLINE | ID: mdl-35285658

RESUMEN

A case series recently published in REED again highlighted the importance of early diagnosis and treatment in preventing complications of esophageal fishbone impaction. As the most common foreign body (FB) in China, fishbone ingestion is frequently encountered in our daily clinical practice. Although the majority could be managed effectively with endoscopy, rare particular cases still present challenges. We described the case of esophageal fishbone in which the first two endoscopic examinations were negative, emphasizing the role of computed tomography in its management.


Asunto(s)
Cuerpos Extraños , China , Endoscopía Gastrointestinal/métodos , Esófago/diagnóstico por imagen , Cuerpos Extraños/complicaciones , Cuerpos Extraños/diagnóstico por imagen , Cuerpos Extraños/cirugía , Humanos , Tomografía Computarizada por Rayos X
8.
Rev Esp Enferm Dig ; 114(7): 438-439, 2022 07.
Artículo en Inglés | MEDLINE | ID: mdl-35199535

RESUMEN

Pancreaticoduodenal artery aneurysm (PDAA) is a rare visceral aneurysm with a high risk of rupture and mortality. Herein, we presented a case of duodenal obstruction associated with a ruptured PDAA during the postoperative course after successful embolization.


Asunto(s)
Aneurisma Roto , Obstrucción Duodenal , Embolización Terapéutica , Aneurisma Roto/complicaciones , Aneurisma Roto/diagnóstico por imagen , Arterias , Obstrucción Duodenal/diagnóstico por imagen , Obstrucción Duodenal/etiología , Duodeno , Humanos , Páncreas/cirugía
9.
Rev Esp Enferm Dig ; 114(3): 173, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-34663074

RESUMEN

A 69-year-old male was referred to our center for further evaluation and treatment of a gastric mass. Esophagogastroduodenoscopy found a 30-mm submucosal tumor (SMT) in the gastric body. Endoscopic ultrasound revealed a hypoechoic lesion originating from the muscularis propria layer. Computed tomography showed that the tumor presented a predominately intraluminal growth pattern.


Asunto(s)
Resección Endoscópica de la Mucosa , Neurilemoma , Neoplasias Gástricas , Anciano , Mucosa Gástrica/patología , Gastroscopía/métodos , Humanos , Masculino , Neurilemoma/diagnóstico por imagen , Neurilemoma/patología , Neurilemoma/cirugía , Estudios Retrospectivos , Neoplasias Gástricas/diagnóstico por imagen , Neoplasias Gástricas/patología , Neoplasias Gástricas/cirugía , Resultado del Tratamiento
12.
BMC Cancer ; 20(1): 1110, 2020 Nov 16.
Artículo en Inglés | MEDLINE | ID: mdl-33198658

RESUMEN

BACKGROUND: People are at a high risk of gastric cancer if their first-degree relatives suffered from atrophic gastritis (AG), intestinal metaplasia (IM), intraepithelial neoplasia (IEN), dysplasia (DYS), or gastric cancer (GC). This study was performed to analyse the association between FDR-GC and GC precursors. METHODS: A cross-sectional study was performed to screen the prevalence of GC precursors from November 2016 to September 2019. A total of 1329 participants with FDR-GC, 193 participants with a family history of non-gastric cancer in FDRs (FDR-nGC), and 860 participants without a family history of cancer in FDRs (FDR-nC) were recruited in this study. The logistic regression model was used in this study. RESULTS: The prevalence of normal, Non-AG, AG/IM, IEN/DYS, and GC was 31.91, 44.21, 13.81, 8.73, and 1.34%, respectively. The prevalence of IEN/DYS was higher in people with FDR-GC and FDR-nGC (FDR-GC: odds ratio (OR) = 1.655; 95%CI, 1.153-2.376; FDR-nGC: OR = 1.984; 95%CI, 1.122-3.506) than those with FDR-nC. The younger the age at which FDRs were diagnosed with GC, the more likely the participants were to develop AG/IM (Ptrend = 0.019). The risk of precursors to GC was higher in participants whose FDR-GC was the mother than in those whose FDR-GC was the father or sibling (OR, non-AG: 1.312 vs. 1.007, 1.274; AG/IM: 1.430 vs. 1.296, 1.378; IEN/DYS: 1.988 vs. 1.573, 1.542). There was no statistically significant difference in non-AG (OR = 1.700; 95%CI, 0.940-3.074), AG/IM (OR = 1.291; 95%CI, 0.579-2.877), and IEN/DYS (OR = 1.265; 95%CI, 0.517-3.096) between participants with one or more FDR-GC. CONCLUSION: People with FDR-GC and FDR-nGC are at a high risk of IEN/DYS. When an FDR was diagnosed at a younger age, the risk of AG/IM was higher. The risk of GC precursors was higher in people whose FDR-GC was the mother.


Asunto(s)
Detección Precoz del Cáncer/métodos , Mucosa Gástrica/patología , Gastritis Atrófica/epidemiología , Predisposición Genética a la Enfermedad , Metaplasia/epidemiología , Lesiones Precancerosas/epidemiología , Neoplasias Gástricas/epidemiología , China/epidemiología , Estudios Transversales , Femenino , Estudios de Seguimiento , Gastritis Atrófica/diagnóstico , Gastritis Atrófica/genética , Gastroscopía , Humanos , Masculino , Metaplasia/diagnóstico , Metaplasia/genética , Persona de Mediana Edad , Lesiones Precancerosas/diagnóstico , Lesiones Precancerosas/genética , Prevalencia , Pronóstico , Factores de Riesgo , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/genética
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