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Invasive alien species (IAS) adversely impact biodiversity, ecosystem functions, and socio-economics. Citizen science can be an effective tool for IAS surveillance, management, and research, providing large datasets over wide spatial extents and long time periods, with public participants generating knowledge that supports action. We demonstrate how citizen science has contributed knowledge across the biological invasion process, especially for early detection and distribution mapping. However, we recommend that citizen science could be used more for assessing impacts and evaluating the success of IAS management. Citizen science does have limitations, and we explore solutions to two key challenges: ensuring data accuracy and dealing with uneven spatial coverage of potential recorders (which limits the dataset's "fit for purpose"). Greater co-development of citizen science with public stakeholders will help us better realize its potential across the biological invasion process and across ecosystems globally while meeting the needs of participants, local communities, scientists, and decision-makers.
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Defining nutrient thresholds that protect and support the ecological integrity of aquatic ecosystems is a fundamental step in maintaining their natural biodiversity and preserving their resilience. With increasing catchment pressures and climate change, it is more important than ever to develop clear methods to establish thresholds for status classification and management of waters. This must often be achieved using complex data and should be robust to interference from additional pressures as well as ameliorating or confounding conditions. We use both artificial and real data to examine challenges in setting nutrient thresholds in unbalanced and skewed data. We found significant advantages to using binary logistic regression over other techniques. However, one of the key challenges is objectively selecting a probability from which to derive the nutrient threshold. For this purpose, the examination of the proportions of matching and mismatching status classifications of nutrients and a biological quality element using a confusion matrix is a key step that should be more widely adopted in threshold selection. We examined a large array of statistical measures of classification accuracy and their performance over combinations of skewness and imbalance in the data. The most appropriate threshold probability is a compromise between maximising overall classification accuracy and reducing mismatches expressed as commission (false positives) without excessive omission (false negatives). An application to a lake type indicated total phosphorus thresholds that would be around 50 µg l-1 lower than the threshold achieved by an 'unguided' approach, indicating that this approach is a very significant development meriting attention from national authorities responsible for water management.
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Ecosistema , Lagos , Biodiversidad , Agua , Nutrientes , FósforoRESUMEN
Tens of millions of images from biological collections have become available online over the last two decades. In parallel, there has been a dramatic increase in the capabilities of image analysis technologies, especially those involving machine learning and computer vision. While image analysis has become mainstream in consumer applications, it is still used only on an artisanal basis in the biological collections community, largely because the image corpora are dispersed. Yet, there is massive untapped potential for novel applications and research if images of collection objects could be made accessible in a single corpus. In this paper, we make the case for infrastructure that could support image analysis of collection objects. We show that such infrastructure is entirely feasible and well worth investing in.
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Community science (also often referred to as citizen science) provides a unique opportunity to address questions beyond the scope of other research methods whilst simultaneously engaging communities in the scientific process. This leads to broad educational benefits, empowers people, and can increase public awareness of societally relevant issues such as the biodiversity crisis. As such, community science has become a favourable framework for researching alien species where data on the presence, absence, abundance, phenology, and impact of species is important in informing management decisions. However, uncertainties arising at different stages can limit the interpretation of data and lead to projects failing to achieve their intended outcomes. Focusing on alien species centered community science projects, we identified key research questions and the relevant uncertainties that arise during the process of developing the study design, for example, when collecting the data and during the statistical analyses. Additionally, we assessed uncertainties from a linguistic perspective, and how the communication stages among project coordinators, participants and other stakeholders can alter the way in which information may be interpreted. We discuss existing methods for reducing uncertainty and suggest further solutions to improve data reliability. Further, we make suggestions to reduce the uncertainties that emerge at each project step and provide guidance and recommendations that can be readily applied in practice. Reducing uncertainties is essential and necessary to strengthen the scientific and community outcomes of community science, which is of particular importance to ensure the success of projects aimed at detecting novel alien species and monitoring their dynamics across space and time. Supplementary Information: The online version contains supplementary material available at 10.1007/s10530-022-02858-8.
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One key component of any eutrophication management strategy is establishment of realistic thresholds above which negative impacts become significant and provision of ecosystem services is threatened. This paper introduces a toolkit of statistical approaches with which such thresholds can be set, explaining their rationale and situations under which each is effective. All methods assume a causal relationship between nutrients and biota, but we also recognise that nutrients rarely act in isolation. Many of the simpler methods have limited applicability when other stressors are present. Where relationships between nutrients and biota are strong, regression is recommended. Regression relationships can be extended to include additional stressors or variables responsible for variation between water bodies. However, when the relationship between nutrients and biota is weaker, categorical approaches are recommended. Of these, binomial regression and an approach based on classification mismatch are most effective although both will underestimate threshold concentrations if a second stressor is present. Whilst approaches such as changepoint analysis are not particularly useful for meeting the specific needs of EU legislation, other multivariate approaches (e.g. decision trees) may have a role to play. When other stressors are present quantile regression allows thresholds to be established which set limits above which nutrients are likely to influence the biota, irrespective of other pressures. The statistical methods in the toolkit may be useful as part of a management strategy, but more sophisticated approaches, often generating thresholds appropriate to individual water bodies rather than to broadly defined "types", are likely to be necessary too. The importance of understanding underlying ecological processes as well as correct selection and application of methods is emphasised, along with the need to consider local regulatory and decision-making systems, and the ease with which outcomes can be communicated to non-technical audiences.
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Ecosistema , Eutrofización , NutrientesRESUMEN
The aim of European water policy is to achieve good ecological status in all rivers, lakes, coastal and transitional waters by 2027. Currently, more than half of water bodies are in a degraded condition and nutrient enrichment is one of the main culprits. Therefore, there is a pressing need to establish reliable and comparable nutrient criteria that are consistent with good ecological status. This paper highlights the wide range of nutrient criteria currently in use by Member States of the European Union to support good ecological status and goes on to suggest that inappropriate criteria may be hindering the achievement of good status. Along with a comprehensive overview of nutrient criteria, we provide a critical analysis of the threshold concentrations and approaches by which these are set. We identify four essential issues: (1) Different nutrients (nitrogen and/or phosphorus) are used for different water categories in different countries. (2) The use of different nutrient fractions (total, dissolved inorganic) and statistical summary metrics (e.g., mean, percentiles, seasonal, annual) currently hampers comparability between countries, particularly for rivers, transitional and coastal waters. (3) Wide ranges in nutrient threshold values within shared water body types, in some cases showing more than a 10-fold difference in concentrations. (4) Different approaches used to set threshold nutrient concentrations to define the boundary between "good" and "moderate" ecological status. Expert judgement-based methods resulted in significantly higher (less stringent) good-moderate threshold values compared with data-driven approaches, highlighting the importance of consistent and rigorous approaches to criteria setting. We suggest that further development of nutrient criteria should be based on relationships between ecological status and nutrient concentrations, taking into account the need for comparability between different water categories, water body types within these categories, and countries.
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Various methods have been proposed to identify threshold concentrations of nutrients that would support good ecological status, but the performance of these methods and the influence of other stressors on the underlying models have not been fully evaluated. We used synthetic datasets to compare the performance of ordinary least squares, logistic and quantile regression, as well as, categorical methods based on the distribution of nutrient concentrations categorised by biological status. The synthetic datasets used differed in their levels of variation between explanatory and response variables, and were centered at different positions along the stressor (nutrient) gradient. In order to evaluate the performance of methods in "multiple stressor" situations, another set of datasets with two stressors was used. Ordinary least squares and logistic regression methods were the most reliable when predicting the threshold concentration when nutrients were the sole stressor; however, both had a tendency to underestimate the threshold when a second stressor was present. In contrast, threshold concentrations produced by categorical methods were strongly influenced by the level of the stressor (nutrient enrichment, in this case) relative to the threshold they were trying to predict (good/moderate in this instance). Although all the methods tested had limitations in the presence of a second stressor, upper quantiles seemed generally appropriate to establish non-precautionary thresholds. For example, upper quantiles may be appropriate when establishing targets for restoration, but not when seeking to minimise deterioration. Selection of an appropriate threshold concentration should also attend to the regulatory regime (i.e. policy requirements and environmental management context) within which it will be used, and the ease of communicating the principles to managers and stakeholders.
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The capacity of ecosystems to supply ecosystem services is decreasing. Sustaining this supply requires an understanding of the links between the impacts of pressures introduced by human activities and how this can lead to changes in the supply of services. Here, we apply a novel approach, assessing 'risk to ecosystem service supply' (RESS), across a range of aquatic ecosystems in seven case studies. We link aggregate impact risk from human activities on ecosystem components, with a relative score of their potential to supply services. The greatest RESS is found where an ecosystem component with a high potential to supply services is subject to high impact risk. In this context, we explore variability in RESS across 99 types of aquatic ecosystem component from 11 realms, ranging from oceanic to wetlands. We explore some causes of variability in the RESS observed, including assessment area, Gross Domestic Product (GDP) and population density. We found that Lakes, Rivers, Inlets and Coastal realms had some of the highest RESS, though this was highly dependent on location. We found a positive relationship between impact risk and service supply potential, indicating the ecosystem components we rely on most for services, are also those most at risk. However, variability in this relationship indicates that protecting the supply of ecosystem services alone will not protect all parts of the ecosystem at high risk. Broad socio-economic factors explained some of the variability found in RESS. For example, RESS was positively associated with GDP and artificial and agricultural land use in most realms, highlighting the need to achieve balance between increasing GDP and sustaining ecosystem health and human wellbeing more broadly. This approach can be used for sustainable management of ecosystem service use, to highlight the ecosystem components most critical to supplying services, and those most at risk.
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Understanding how changes in biodiversity affects ecosystem functioning is imperative in allowing Ecosystem-Based Management (EBM), especially when addressing global change and environmental degradation. Research into the link between biodiversity and ecosystem functioning (BEF) has indeed increased considerably over the past decades. BEF research has focussed on terrestrial ecosystems and aquatic ecosystems have received considerably less attention. Due to differences in phylogenetic diversity, ecological processes and reported BEF relationships, however, it may at least be questionable whether BEF relationships are exchangeable between these ecosystems (i.e. terrestrial and aquatic). The aim of the present paper was therefore to pinpoint key areas and bottlenecks in establishing BEF relationships for aquatic ecosystems (freshwater, transitional, and marine). To this end, the available literature with special emphasis on the last 10â¯years was assessed to evaluate: i) reported mechanisms and shapes of aquatic BEF relationships; ii) to what extent BEF relations are interchangeable or ecosystem-specific; and iii) contemporary gaps and needs in aquatic BEF research. Based on our analysis, it may be concluded that despite considerable progress in BEF research over the past decades, several bottlenecks still need to be tackled, namely incorporating the multitude of functions supported by ecosystems, functional distinctiveness of rare species, multitrophic interactions and spatial-temporal scales, before BEF relationships can be used in ecosystem-based management.
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Organismos Acuáticos/fisiología , Biodiversidad , EcosistemaRESUMEN
The Baixo Vouga Lagunar (BVL) is part of Ria de Aveiro coastal lagoon in Portugal, which is classified as a Special Protection Area under the European Habitats and Birds Directives. This part of the system, corresponding to the confluence of the Vouga River with the lagoon, is very important culturally and socioeconomically for the local communities, taking place several human activities, especially agriculture. To prevent salt water intrusion from the Ria de Aveiro into agriculture fields, a floodbank was initiated in the 90's. In frame of ongoing changes in Ria de Aveiro hydrodynamics, the existing floodbank will be now extended, introducing further changes in the ecological dynamics of the BVL and its adjacent area. As a consequence, the water level in the floodbank downstream side is expected to rise, increasing the submersion period in tidal wetlands, and leading to coastal squeeze. The aim of this study is to apply an ecosystem based-management approach to mitigate the impacts on biodiversity resulting from the management plan. To do so, we have modelled the implications of the changes in several hydrological and environmental variables on four saltmarsh species and habitats distribution, as well as on their associated ecosystem services, both upstream and downstream of the floodbank. The ecosystem services of interest were prioritized by stakeholders' elicitation, which were then used as an input to a spatial multi-criteria analysis aimed to find the best management actions to compensate for the unintended loss of biodiversity and ecosystem services in the BVL. According to our results, the main areas to be preserved in the BVL were the traditional agricultural mosaic fields; the freshwater courses and the subtidal estuarine channels. By combining ecology with the analysis of social preferences, this study shows how co-developed solutions can support adaptive management and the conservation of coastal ecosystems.
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Aquatic ecosystems are under severe pressure. Human activities introduce an array of pressures that impact ecosystems and their components. In this study we focus on the aquatic domains of fresh, coastal and marine waters, including rivers, lakes and riparian habitats to transitional, coastal as well as shelf and oceanic habitats. In an environmental risk assessment approach, we identified impact chains that link 45 human activities through 31 pressures to 82 ecosystem components. In this linkage framework >22,000 activity-pressure-ecosystem component interactions were found across seven European case studies. We identified the environmental impact risk posed by each impact chain by first categorically weighting the interactions according to five criteria: spatial extent, dispersal potential, frequency of interaction, persistence of pressure and severity of the interaction, where extent, dispersal, frequency and persistence account for the exposure to risk (spatial and temporal), and the severity accounts for the consequence of the risk. After assigning a numerical score to each risk criterion, we came up with an overall environmental impact risk score for each impact chain. This risk score was analysed in terms of (1) the activities and pressures that introduce the greatest risk to European aquatic domains, and (2) the aquatic ecosystem components and realms that are at greatest risk from human activities. Activities related to energy production were relevant across the aquatic domains. Fishing was highly relevant in marine and environmental engineering in fresh waters. Chemical and physical pressures introduced the greatest risk to the aquatic realms. Ecosystem components that can be seen as ecotones between different ecosystems had high impact risk. We show how this information can be used in informing management on trade-offs in freshwater, coastal and marine resource use and aid decision-making.
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Conservación de los Recursos Hídricos/métodos , Ecosistema , Agua Dulce/química , Actividades Humanas/tendencias , Agua de Mar/química , Contaminación del Agua/análisis , Organismos Acuáticos/efectos de los fármacos , Humanos , Medición de Riesgo , Contaminación del Agua/efectos adversosRESUMEN
Green and Blue Infrastructure (GBI) is a network designed and planned to deliver a wide range of ecosystem services and to protect biodiversity. Existing GBI designs lacked a systematic method to allocate restoration zones. This study proposes a novel approach for systematically selecting cost-effective areas for restoration on the basis of biodiversity, ecosystem services, and ecosystem condition to give an optimal spatial design of GBI. The approach was tested at a regional scale, in a transboundary setting encompassing the Intercontinental Biosphere Reserve of the Mediterranean in Andalusia (Spain) - Morocco (IBRM), across three aquatic ecosystems: freshwater, coastal and marine. We applied Marxan with Zones to stakeholder-defined scenarios of GBI in the IBRM. Specifically, we aimed to identify management zones within the GBl that addressed different conservation, restoration and exploitation objectives. Although almost all conservation targets were achieved, our results highlighted that the proportion of conservation features (i.e., biodiversity, ecosystem services) that would be compromised in the GBl, and the proportion of provisioning services that would be lost due to conservation (i.e., incidental representation) are potentially large, indicating that the probability of conflicts between conservation and exploitation goals in the area is high. The implementation of restoration zones improved connectivity across the GBI, and also achieved European and global policy targets. Our approach may help guide future applications of GBI to implement the flexible conservation management that aquatic environments require, considering many areas at different spatial scales, across multiple ecosystems, and in transboundary contexts.
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Ria de Aveiro represents a coastal territory, in which its natural capital, mostly classified under a Natura 2000 network of protected areas, is of paramount importance for the regional and national economy, supporting harbour activities and maritime traffic, agriculture, commercial fisheries, aquaculture, manufacturing, tourism, sports and recreational activities. Current and foreseen changes connected to human activities, namely land and water uses and potential conflicts, in frame of environmental policies, sustainable economic development and human well-being require the implementation of ecosystem-based management (EBM) planning processes considering the connectivity across marine, transitional, freshwater, and terrestrial domains. The main objective is to elaborate on the co-development of the EBM planning process across the three water domains, all characterized by high biodiversity and by the wide range of services provided by ecosystems and their abiotic components, for the mitigation of impacts from the management plan under implementation. The approach used follows a stepwise procedure in frame of resilience principles, considering the analysis of the relationship between the social and ecological components and on how these can be connected through risk assessment and a spatial multi-criteria analysis based on the delivery of ecosystem services. Stakeholders' perception matched the ecosystem services provisioning risk assessment and supported the planning EBM response that consist in saltmarshes and seagrasses meadows restoration programs. Compliance of the proposed measures is achievable regarding policies (policy targets and policy instruments) and feasibility (scientific and technological knowledge and financial resources). The EBM response can support the Vouga estuary management plan and regional smart specialization (RIS3 Centro).
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Global initiatives have been increasingly focusing on mainstreaming the values of biodiversity and ecosystem services into decision-making at all levels. Due to the accelerated rate at which biodiversity is declining and its consequences for the functioning of ecosystems and subsequently, the services they provide, there is need to develop comprehensive assessments of the services and the benefits nature delivers to society. Based on expert evaluation, we identified relevant flow linkages in the supply-side of the socio-ecological system, i.e. from biodiversity to ecosystem services supply for eight case studies across European aquatic ecosystems covering freshwater, transitional, coastal and marine waters realms. Biological mediated services were considered, as well as those reliant on purely physical aspects of the ecosystem, i.e. abiotic outputs, since both have implications for spatial planning, management and decision-making. Due to the multidimensional nature of ecosystems and their biodiversity, our approach used ecosystem components such as habitats and biota as proxies for biodiversity and as the focal point for linkage identification. Statistical analysis revealed the importance of considering mobile biota in the spatial assessment of habitats. Contrary to literature evidences so far, our results showed significantly different and complementary ecosystem services supply patterns across the continuum of aquatic realms. The implemented score of ecosystem services supply has a high potential for integrated aquatic ecosystem service supply assessments in the context of ecosystem-based management.
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Conservación de los Recursos Naturales/métodos , Ecosistema , Política Ambiental , Biodiversidad , Europa (Continente) , Agua Dulce , Modelos Teóricos , Marruecos , Agua de MarRESUMEN
The European Marine Strategy Framework Directive aims at good environmental status (GES) in marine waters, following an ecosystem-based approach, focused on 11 descriptors related to ecosystem features, human drivers and pressures. Furthermore, 29 subordinate criteria and 56 attributes are detailed in an EU Commission Decision. The analysis of the Decision and the associated operational indicators revealed ambiguity in the use of terms, such as indicator, impact and habitat and considerable overlap of indicators assigned to various descriptors and criteria. We suggest re-arrangement and elimination of redundant criteria and attributes avoiding double counting in the subsequent indicator synthesis, a clear distinction between pressure and state descriptors and addition of criteria on ecosystem services and functioning. Moreover, we suggest the precautionary principle should be followed for the management of pressures and an evidence-based approach for monitoring state as well as reaching and maintaining GES.
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Conservación de los Recursos Naturales , Ecosistema , Monitoreo del Ambiente , Política Ambiental , Océanos y Mares , Conservación de los Recursos Naturales/legislación & jurisprudencia , Conservación de los Recursos Naturales/métodos , Monitoreo del Ambiente/legislación & jurisprudencia , Monitoreo del Ambiente/métodos , Europa (Continente)RESUMEN
Land use and land cover (LULC) are driving forces that potentially exert pressures on water bodies, which are most commonly quantified by simply obtained aggregated data. However, this is insufficient to detect the drivers that arise from the landscape change itself. To achieve this objective one must distinguish between random and systematic transitions and identify the transitions that show strong signals of change, since these will make it possible to identify the transitions that have evolved due to population growth, industrial expansion and/or changes in land management policies. Our goal is to describe a method to characterize driving forces both from LULC and dominant LULC changes, recognizing that the presence of certain LULC classes as well as the processes of transition to other uses are both sources of stress with potential effects on the condition of water bodies. This paper first quantifies the driving forces from LULC and also from processes of LULC change for three nested regions within the Mondego river basin in 1990, 2000 and 2006. It then discusses the implications for the environmental water body condition and management policies. The fingerprint left on the landscape by some of the dominant changes found, such as urbanization and industrial expansion, is, as expected, low due to their proportion in the geographic regions under study, yet their magnitude of change and consistency reveal strong signals of change regarding the pressures acting in the system. Assessing dominant LULC changes is vital for a comprehensive study of driving forces with potential impacts on water condition.
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Monitoreo del Ambiente/métodos , Contaminación del Agua/estadística & datos numéricos , Agricultura/estadística & datos numéricos , Sistemas de Información Geográfica , Crecimiento Demográfico , Urbanización/tendencias , Contaminación del Agua/análisis , Calidad del AguaRESUMEN
The European Marine Strategy Framework Directive (MSFD) requires EU Member States (MS) to achieve Good Environmental Status (GEnS) of their seas by 2020. We address the question of what GEnS entails especially with regard to the level at which targets are set (descriptors, criteria, indicators), to scales for assessments (regional, sub-divisions, site-specific), and to difficulties in putting into practice the GEnS concept. We propose a refined and operational definition of GEnS, indicating the data and information needed to all parts of that definition. We indicate the options for determining when GEnS has been met, acknowledge the data and information needs for each option, and recommend a combination of existing quantitative targets and expert judgement. We think that the MSFD implementation needs to be less complex than shown for other similar directives, can be based largely on existing data and can be centred on the activities of the Regional Seas Conventions.
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Conservación de los Recursos Naturales , Ecosistema , Contaminación del Agua/estadística & datos numéricos , Monitoreo del Ambiente , Política Ambiental , Océanos y Mares , Contaminación del Agua/prevención & controlRESUMEN
The concept of Ecological Potential was explored using the macrobenthic communities of the Mondego estuary (Portugal). Different scenarios (loss of a primary producer and intertidal habitat, and habitat modification due to hydromorphological changes and continued press perturbation) were tested to predict changes in the biology if hydromorphological pressures could be reversed, assuming that differences with and without the pressure could indicate the potential. Results showed noticeable changes in the system biology in each scenario. The approach followed, indicates that when data sets exist, differences in the measurement of ecological status with and without the hydromorphological change could be a way forward to determine the potential. In the particular case of the Mondego estuary, the South arm (physically unaltered water body) proved to be richer than the North (HMWB). For the Ecological Potential determination, the South arm could thus be used to derive and adjust future reference conditions for the North.
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Monitoreo del Ambiente/métodos , Estuarios , Biodiversidad , Ecología , Política Ambiental , PortugalRESUMEN
In recent times many benthic indices have been proposed to assess the ecological quality of marine waters worldwide. In this study we compared single metrics and multi-metric methods to assess coastal and transitional benthic status along human pressure gradients in five distinct environments across Europe: Varna bay and lake (Bulgaria), Lesina lagoon (Italy), Mondego estuary (Portugal), Basque coast (Spain) and Oslofjord (Norway). Hence, 13 single metrics (abundance, number of taxa, and several diversity and sensitivity indices) and eight of the most common indices used within the European Water Framework Directive (WFD) for benthic assessment were selected: index of size spectra (ISS), Benthic assessment tool (BAT), Norwegian quality index (NQI), Multivariate AMBI (M-AMBI), Benthic quality index (BQI), (Benthic ecosystem quality index (BEQI), Benthic index based on taxonomic sufficiency (BITS), and infaunal quality index (IQI). Within each system, sampling sites were ordered in an increasing pressure gradient according to a preliminary classification based on professional judgement. The different indices are largely consistent in their response to pressure gradient, except in some particular cases (i.e. BITS, in all cases, or ISS when a low number of individuals is present). Inconsistencies between indicator responses were most pronounced in transitional waters (i.e. IQI, BEQI), highlighting the difficulties of the generic application of indicators to all marine, estuarine and lagoonal environments. However, some of the single (i.e. ecological groups approach, diversity, richness) and multi-metric methods (i.e. BAT, M-AMBI, NQI) were able to detect such gradients both in transitional and coastal environments, being these multi-metric methods more consistent in the detection than single indices. This study highlights the importance of survey design and good reference conditions for some indicators. The agreement observed between different methodologies and their ability to detect quality trends across distinct environments constitutes a promising result for the implementation of the WFD's monitoring plans. Moreover, these results have management implications, regarding the dangers of misclassification, uncertainty in the assessment, use of conflicting indices, and testing and validation of indices.
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Ecosistema , Monitoreo del Ambiente/métodos , Contaminantes Químicos del Agua/análisis , Contaminación Química del Agua/estadística & datos numéricos , Biodiversidad , Europa (Continente) , Océanos y Mares , Agua de Mar/química , Contaminantes Químicos del Agua/normasRESUMEN
Benthic indices are typically developed independently by habitat, making their incorporation into large geographic scale assessments potentially problematic because of scaling inequities. A potential solution is to establish common scaling using expert best professional judgment (BPJ). To test if experts from different geographies agree on condition assessment, sixteen experts from four regions in USA and Europe were provided species-abundance data for twelve sites per region. They ranked samples from best to worst condition and classified samples into four condition (quality) categories. Site rankings were highly correlated among experts, regardless of whether they were assessing samples from their home region. There was also good agreement on condition category, though agreement was better for samples at extremes of the disturbance gradient. The absence of regional bias suggests that expert judgment is a viable means for establishing a uniform scale to calibrate indices consistently across geographic regions.