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1.
J Integr Plant Biol ; 66(7): 1295-1312, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38695649

RESUMEN

Cultivating high-yield wheat under limited water resources is crucial for sustainable agriculture in semiarid regions. Amid water scarcity, plants activate drought response signaling, yet the delicate balance between drought tolerance and development remains unclear. Through genome-wide association studies and transcriptome profiling, we identified a wheat atypical basic helix-loop-helix (bHLH) transcription factor (TF), TabHLH27-A1, as a promising quantitative trait locus candidate for both relative root dry weight and spikelet number per spike in wheat. TabHLH27-A1/B1/D1 knock-out reduced wheat drought tolerance, yield, and water use efficiency (WUE). TabHLH27-A1 exhibited rapid induction with polyethylene glycol (PEG) treatment, gradually declining over days. It activated stress response genes such as TaCBL8-B1 and TaCPI2-A1 while inhibiting root growth genes like TaSH15-B1 and TaWRKY70-B1 under short-term PEG stimulus. The distinct transcriptional regulation of TabHLH27-A1 involved diverse interacting factors such as TaABI3-D1 and TabZIP62-D1. Natural variations of TabHLH27-A1 influence its transcriptional responses to drought stress, with TabHLH27-A1Hap-II associated with stronger drought tolerance, larger root system, more spikelets, and higher WUE in wheat. Significantly, the excellent TabHLH27-A1Hap-II was selected during the breeding process in China, and introgression of TabHLH27-A1Hap-II allele improved drought tolerance and grain yield, especially under water-limited conditions. Our study highlights TabHLH27-A1's role in balancing root growth and drought tolerance, providing a genetic manipulation locus for enhancing WUE in wheat.


Asunto(s)
Sequías , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas , Raíces de Plantas , Triticum , Agua , Triticum/genética , Triticum/crecimiento & desarrollo , Triticum/fisiología , Triticum/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Agua/metabolismo , Sitios de Carácter Cuantitativo/genética , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Estrés Fisiológico/genética , Estudio de Asociación del Genoma Completo , Resistencia a la Sequía
2.
J Integr Plant Biol ; 66(6): 1242-1260, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38656698

RESUMEN

Leaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical role in regulating leaf senescence, and also affected spike length and grain size. Furthermore, the functional divergences among the three haplotypes of TaNAM-A1 were systematically evaluated. Wheat varieties with TaNAM-A1d (containing two mutations in the coding DNA sequence of TaNAM-A1) exhibited a longer GLD and superior yield-related traits compared to those with the wild type TaNAM-A1a. All three haplotypes were functional in activating the expression of genes involved in macromolecule degradation and mineral nutrient remobilization, with TaNAM-A1a showing the strongest activity and TaNAM-A1d the weakest. TaNAM-A1 also modulated the expression of the senescence-related transcription factors TaNAC-S-7A and TaNAC016-3A. TaNAC016-3A enhanced the transcriptional activation ability of TaNAM-A1a by protein-protein interaction, thereby promoting the senescence process. Our study offers new insights into the fine-tuning of the leaf functional period and grain yield formation for wheat breeding under various geographical climatic conditions.


Asunto(s)
Grano Comestible , Regulación de la Expresión Génica de las Plantas , Haplotipos , Hojas de la Planta , Proteínas de Plantas , Triticum , Triticum/genética , Triticum/fisiología , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Hojas de la Planta/fisiología , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Haplotipos/genética , Grano Comestible/genética , Grano Comestible/crecimiento & desarrollo , Senescencia de la Planta/genética , Genes de Plantas , Variación Genética , Fenotipo
3.
Plant Cell Environ ; 47(8): 2954-2970, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38629794

RESUMEN

Increasing the tolerance of crops to water deficit is crucial for the improvement of crop production in water-restricted regions. Here, a wheat peroxidase gene (TaPrx109-B1) belonging to the class III peroxidase gene family was identified and its function in water deficit tolerance was revealed. We demonstrated that overexpression of TaPrx109-B1 reduced leaf H2O2 level and stomatal density, increased leaf relative water content, water use efficiency, and tolerance to water deficit. The expression of TaEPF1 and TaEPF2, two key negative regulators of stomatal development, were significantly upregulated in TaPrx109-B1 overexpression lines. Furthermore, exogenous H2O2 downregulated the expression of TaEPF1 and TaEPF2 and increased stomatal density, while exogenous application of diphenyleneiodonium chloride, a potent NADPH oxidase inhibitor that repressed the synthesis of H2O2, upregulated the expression of TaEPF1 and TaEPF2, decreased stomatal density, and enhanced wheat tolerance to water deficit. These findings suggest that TaPrx109-B1 influences leaf stomatal density by modulation of H2O2 level and the expression of TaEPF1 and TaEPF2. The results of the field trial showed that overexpressing TaPrx109-B1 increased grain number per spike, which reduced the yield loss caused by water deficiency. Therefore, TaPrx109-B1 has great potential in breeding wheat varieties with improved water deficit tolerance.


Asunto(s)
Peróxido de Hidrógeno , Proteínas de Plantas , Estomas de Plantas , Plantas Modificadas Genéticamente , Triticum , Triticum/genética , Triticum/fisiología , Estomas de Plantas/fisiología , Estomas de Plantas/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Peróxido de Hidrógeno/metabolismo , Agua/metabolismo , Regulación de la Expresión Génica de las Plantas , Sequías , Peroxidasa/metabolismo , Peroxidasa/genética , Hojas de la Planta/fisiología , Hojas de la Planta/genética , Deshidratación
4.
New Phytol ; 242(2): 641-657, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38379453

RESUMEN

Nitrate is the main source of nitrogen (N) available to plants and also is a signal that triggers complex regulation of transcriptional networks to modulate a wide variety of physiological and developmental responses in plants. How plants adapt to soil nitrate fluctuations is a complex process involving a fine-tuned response to nitrate provision and N starvation, the molecular mechanisms of which remain largely uncharted. Here, we report that the wheat transcription factor TaLBD41 interacts with the nitrate-inducible transcription factor TaNAC2 and is repressed by nitrate provision. Electrophoretic mobility shift assay and dual-luciferase system show that the TaLBD41-NAC2 interaction confers homeostatic coordination of nitrate uptake, reduction, and assimilation by competitively binding to TaNRT2.1, TaNR1.2, and TaNADH-GOGAT. Knockdown of TaLBD41 expression enhances N uptake and assimilation, increases spike number, grain yield, and nitrogen harvest index under different N supply conditions. We also identified an elite haplotype of TaLBD41-2B associated with increased spike number and grain yield. Our study uncovers a novel mechanism underlying the interaction between two transcription factors in mediating wheat adaptation to nitrate availability by antagonistically regulating nitrate uptake and assimilation, providing a potential target for designing varieties with efficient N use in wheat (Triticum aestivum).


Asunto(s)
Nitratos , Nitrógeno , Nitratos/metabolismo , Nitrógeno/metabolismo , Transporte Biológico , Grano Comestible/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo
5.
Nat Commun ; 14(1): 7465, 2023 11 17.
Artículo en Inglés | MEDLINE | ID: mdl-37978184

RESUMEN

Transposable elements (TEs) comprise ~85% of the common wheat genome, which are highly diverse among subgenomes, possibly contribute to polyploid plasticity, but the causality is only assumed. Here, by integrating data from gene expression cap analysis and epigenome profiling via hidden Markov model in common wheat, we detect a large proportion of enhancer-like elements (ELEs) derived from TEs producing nascent noncoding transcripts, namely ELE-RNAs, which are well indicative of the regulatory activity of ELEs. Quantifying ELE-RNA transcriptome across typical developmental stages reveals that TE-initiated ELE-RNAs are mainly from RLG_famc7.3 specifically expanded in subgenome A. Acquisition of spike-specific transcription factor binding likely confers spike-specific expression of RLG_famc7.3-initiated ELE-RNAs. Knockdown of RLG_famc7.3-initiated ELE-RNAs resulted in global downregulation of spike-specific genes and abnormal spike development. These findings link TE expansion to regulatory specificity and polyploid developmental plasticity, highlighting the functional impact of TE-driven regulatory innovation on polyploid evolution.


Asunto(s)
Elementos Transponibles de ADN , Triticum , Elementos Transponibles de ADN/genética , Triticum/genética , Regulación de la Expresión Génica , Poliploidía , Transcriptoma , ARN
6.
Nat Commun ; 14(1): 7538, 2023 Nov 20.
Artículo en Inglés | MEDLINE | ID: mdl-37985755

RESUMEN

Polyploidization is a major driver of genome diversification and environmental adaptation. However, the merger of different genomes may result in genomic conflicts, raising a major question regarding how genetic diversity is interpreted and regulated to enable environmental plasticity. By analyzing the genome-wide binding of 191 trans-factors in allopolyploid wheat, we identified like heterochromatin protein 1 (LHP1) as a master regulator of subgenome-diversified genes. Transcriptomic and epigenomic analyses of LHP1 mutants reveal its role in buffering the expression of subgenome-diversified defense genes by controlling H3K27me3 homeostasis. Stripe rust infection releases latent subgenomic variations by eliminating H3K27me3-related repression. The simultaneous inactivation of LHP1 homoeologs by CRISPR-Cas9 confers robust stripe rust resistance in wheat seedlings. The conditional repression of subgenome-diversified defenses ensures developmental plasticity to external changes, while also promoting neutral-to-non-neutral selection transitions and adaptive evolution. These findings establish an LHP1-mediated buffering system at the intersection of genotypes, environments, and phenotypes in polyploid wheat. Manipulating the epigenetic buffering capacity offers a tool to harness cryptic subgenomic variations for crop improvement.


Asunto(s)
Epigenómica , Triticum , Triticum/genética , Triticum/metabolismo , Histonas/metabolismo , Epigénesis Genética , Genoma de Planta/genética
8.
Sci China Life Sci ; 66(4): 819-834, 2023 04.
Artículo en Inglés | MEDLINE | ID: mdl-36417050

RESUMEN

Expression divergence caused by genetic variation and crosstalks among subgenomes of the allohexaploid bread wheat (Triticum aestivum. L., BBAADD) is hypothesized to increase its adaptability and/or plasticity. However, the molecular basis of expression divergence remains unclear. Squamosa promoter-binding protein-like (SPL) transcription factors are critical for a wide array of biological processes. In this study, we constructed expression regulatory networks by combining DAP-seq for 40 SPLs, ATAC-seq, and RNA-seq. Our findings indicate that a group of low-affinity SPL binding regions (SBRs) were targeted by diverse SPLs and caused different sequence preferences around the core GTAC motif. The SBRs including the low-affinity ones are evolutionarily conserved, enriched GWAS signals related to important agricultural traits. However, those SBRs are highly diversified among the cis-regulatory regions (CREs) of syntenic genes, with less than 8% SBRs coexisting in triad genes, suggesting that CRE variations are critical for subgenome differentiations. Knocking out of TaSPL7A/B/D and TaSPL15A/B/D subfamily further proved that both high- and low-affinity SBRs played critical roles in the differential expression of genes regulating tiller number and spike sizes. Our results have provided baseline data for downstream networks of SPLs and wheat improvements and revealed that CRE variations are critical sources for subgenome divergence in the allohexaploid wheat.


Asunto(s)
Genoma de Planta , Triticum , Triticum/genética , Fenotipo , Sitios de Unión , Regulación de la Expresión Génica de las Plantas
9.
Nat Commun ; 13(1): 6940, 2022 11 14.
Artículo en Inglés | MEDLINE | ID: mdl-36376315

RESUMEN

The success of common wheat as a global staple crop was largely attributed to its genomic diversity and redundancy due to the merge of different genomes, giving rise to the major question how subgenome-divergent and -convergent transcription is mediated and harmonized in a single cell. Here, we create a catalog of genome-wide transcription factor-binding sites (TFBSs) to assemble a common wheat regulatory network on an unprecedented scale. A significant proportion of subgenome-divergent TFBSs are derived from differential expansions of particular transposable elements (TEs) in diploid progenitors, which contribute to subgenome-divergent transcription. Whereas subgenome-convergent transcription is associated with balanced TF binding at loci derived from TE expansions before diploid divergence. These TFBSs have retained in parallel during evolution of each diploid, despite extensive unbalanced turnover of the flanking TEs. Thus, the differential evolutionary selection of paleo- and neo-TEs contribute to subgenome-convergent and -divergent regulation in common wheat, highlighting the influence of TE repertory plasticity on transcriptional plasticity in polyploid.


Asunto(s)
Elementos Transponibles de ADN , Triticum , Elementos Transponibles de ADN/genética , Triticum/genética , Genoma de Planta/genética , Poliploidía , Diploidia , Evolución Molecular
10.
Plant Commun ; 3(4): 100304, 2022 07 11.
Artículo en Inglés | MEDLINE | ID: mdl-35605195

RESUMEN

Triticeae species, including wheat, barley, and rye, are critical for global food security. Mapping agronomically important genes is crucial for elucidating molecular mechanisms and improving crops. However, Triticeae includes many wild relatives with desirable agronomic traits, and frequent introgressions occurred during Triticeae evolution and domestication. Thus, Triticeae genomes are generally large and complex, making the localization of genes or functional elements that control agronomic traits challenging. Here, we developed Triti-Map, which contains a suite of user-friendly computational packages specifically designed and optimized to overcome the obstacles of gene mapping in Triticeae, as well as a web interface integrating multi-omics data from Triticeae for the efficient mining of genes or functional elements that control particular traits. The Triti-Map pipeline accepts both DNA and RNA bulk-segregated sequencing data as well as traditional QTL data as inputs for locating genes and elucidating their functions. We illustrate the usage of Triti-Map with a combination of bulk-segregated ChIP-seq data to detect a wheat disease-resistance gene with its promoter sequence that is absent from the reference genome and clarify its evolutionary process. We hope that Triti-Map will facilitate gene isolation and accelerate Triticeae breeding.


Asunto(s)
Evolución Molecular , Genoma de Planta , Fitomejoramiento , Poaceae/genética , Triticum/genética
11.
Plants (Basel) ; 11(4)2022 Feb 11.
Artículo en Inglés | MEDLINE | ID: mdl-35214826

RESUMEN

The increasing global population and the negative effects of nitrogen (N) fertilizers on the environment challenge wheat breeding to maximize yield potential and grain protein concentration (GPC) in an economically and environmentally friendly manner. Understanding the molecular mechanisms for the response of yield components to N availability and assimilates allocation to grains provides the opportunity to increase wheat yield and GPC simultaneously. This review summarized quantitative trait loci/genes which can increase spikes and grain number by enhancing N uptake and assimilation at relative early growth stage, and 1000-grain weight and GPC by increasing post-anthesis N uptake and N allocation to grains.

12.
Genome Res ; 31(12): 2276-2289, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34503979

RESUMEN

More than 80% of the wheat genome consists of transposable elements (TEs), which act as major drivers of wheat genome evolution. However, their contributions to the regulatory evolution of wheat adaptations remain largely unclear. Here, we created genome-binding maps for 53 transcription factors (TFs) underlying environmental responses by leveraging DAP-seq in Triticum urartu, together with epigenomic profiles. Most TF binding sites (TFBSs) located distally from genes are embedded in TEs, whose functional relevance is supported by purifying selection and active epigenomic features. About 24% of the non-TE TFBSs share significantly high sequence similarity with TE-embedded TFBSs. These non-TE TFBSs have almost no homologous sequences in non-Triticeae species and are potentially derived from Triticeae-specific TEs. The expansion of TE-derived TFBS linked to wheat-specific gene responses, suggesting TEs are an important driving force for regulatory innovations. Altogether, TEs have been significantly and continuously shaping regulatory networks related to wheat genome evolution and adaptation.

13.
Plant Cell ; 33(4): 865-881, 2021 05 31.
Artículo en Inglés | MEDLINE | ID: mdl-33594406

RESUMEN

Wheat (Triticum aestivum) has a large allohexaploid genome. Subgenome-divergent regulation contributed to genome plasticity and the domestication of polyploid wheat. However, the specificity encoded in the wheat genome determining subgenome-divergent spatio-temporal regulation has been largely unexplored. The considerable size and complexity of the genome are major obstacles to dissecting the regulatory specificity. Here, we compared the epigenomes and transcriptomes from a large set of samples under diverse developmental and environmental conditions. Thousands of distal epigenetic regulatory elements (distal-epiREs) were specifically linked to their target promoters with coordinated epigenomic changes. We revealed that subgenome-divergent activity of homologous regulatory elements is affected by specific epigenetic signatures. Subgenome-divergent epiRE regulation of tissue specificity is associated with dynamic modulation of H3K27me3 mediated by Polycomb complex and demethylases. Furthermore, quantitative epigenomic approaches detected key stress responsive cis- and trans-acting factors validated by DNA Affinity Purification and sequencing, and demonstrated the coordinated interplay between epiRE sequence contexts, epigenetic factors, and transcription factors in regulating subgenome divergent transcriptional responses to external changes. Together, this study provides a wealth of resources for elucidating the epiRE regulomics and subgenome-divergent regulation in hexaploid wheat, and gives new clues for interpreting genetic and epigenetic interplay in regulating the benefits of polyploid wheat.


Asunto(s)
Epigénesis Genética , Secuencias Reguladoras de Ácidos Nucleicos , Estrés Fisiológico/genética , Triticum/genética , Regulación de la Expresión Génica de las Plantas , Genoma de Planta , Histonas/genética , Histonas/metabolismo , Lisina/genética , Lisina/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Triticum/fisiología
14.
BMC Plant Biol ; 21(1): 27, 2021 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-33413113

RESUMEN

BACKGROUND: Soil salinization is a major threat to wheat production. It is essential to understand the genetic basis of salt tolerance for breeding and selecting new salt-tolerant cultivars that have the potential to increase wheat yield. RESULT: In this study, a panel of 191 wheat accessions was subjected to genome wide association study (GWAS) to identify SNP markers linked with adult-stage characters. The population was genotyped by Wheat660K SNP array and eight phenotype traits were investigated under low and high salinity environments for three consecutive years. A total of 389 SNPs representing 11 QTLs were significantly associated with plant height, spike number, spike length, grain number, thousand kernels weight, yield and biological mass under different salt treatments, with the phenotypic explanation rate (R2) ranging from 9.14 to 50.45%. Of these, repetitive and pleiotropic loci on chromosomes 4A, 5A, 5B and 7A were significantly linked to yield and yield related traits such as thousand kernels weight, spike number, spike length, grain number and so on under low salinity conditions. Spike length-related loci were mainly located on chromosomes 1B, 3B, 5B and 7A under different salt treatments. Two loci on chromosome 4D and 5A were related with plant height in low and high salinity environment, respectively. Three salt-tolerant related loci were confirmed to be important in two bi-parental populations. Distribution of favorable haplotypes indicated that superior haplotypes of pleiotropic loci on group-5 chromosomes were strongly selected and had potential for increasing wheat salt tolerance. A total of 14 KASP markers were developed for nine loci associating with yield and related traits to improve the selection efficiency of wheat salt-tolerance breeding. CONCLUSION: Utilizing a Wheat660K SNPs chip, QTLs for yield and its related traits were detected under salt treatment in a natural wheat population. Important salt-tolerant related loci were validated in RIL and DH populations. This study provided reliable molecular markers that could be crucial for marker-assisted selection in wheat salt tolerance breeding programs.


Asunto(s)
Producción de Cultivos/estadística & datos numéricos , Grano Comestible/genética , Variación Genética , Estudio de Asociación del Genoma Completo , Estrés Salino/genética , Tolerancia a la Sal/genética , Triticum/genética , China , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Genotipo , Fenotipo
15.
New Phytol ; 225(4): 1667-1680, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31581317

RESUMEN

Seed vigour and early establishment are important factors determining the yield of crops. A wheat nitrate-inducible NAC transcription factor, TaNAC2, plays a critical role in promoting crop growth and nitrogen use efficiency (NUE), and now its role in seed vigour is revealed. A TaNAC2 regulated gene was identified that is a NRT2-type nitrate transporter TaNRT2.5 with a key role in seed vigour. Overexpressing TaNAC2-5A increases grain nitrate concentration and seed vigour by directly binding to the promoter of TaNRT2.5-3B and positively regulating its expression. TaNRT2.5 is expressed in developing grain, particularly the embryo and husk. In Xenopus oocyte assays TaNRT2.5 requires a partner protein TaNAR2.1 to give nitrate transport activity, and the transporter locates to the tonoplast in a tobacco leaf transient expression system. Furthermore, in the root TaNRT2.5 and TaNRT2.1 function in post-anthesis acquisition of soil nitrate. Overexpression of TaNRT2.5-3B increases seed vigour, grain nitrate concentration and yield, whereas RNA interference of TaNRT2.5 has the opposite effects. The TaNAC2-NRT2.5 module has a key role in regulating grain nitrate accumulation and seed vigour. Both genes can potentially be used to improve grain yield and NUE in wheat.


Asunto(s)
Nitratos/metabolismo , Proteínas de Plantas/metabolismo , Semillas/fisiología , Factores de Transcripción/metabolismo , Triticum/metabolismo , Animales , Transporte Biológico , Regulación de la Expresión Génica de las Plantas/fisiología , Oocitos/metabolismo , Proteínas de Plantas/genética , Transporte de Proteínas , Transducción de Señal , Factores de Transcripción/genética , Triticum/genética , Xenopus
16.
Genome Biol ; 20(1): 139, 2019 07 15.
Artículo en Inglés | MEDLINE | ID: mdl-31307500

RESUMEN

BACKGROUND: Bread wheat is an allohexaploid species with a 16-Gb genome that has large intergenic regions, which presents a big challenge for pinpointing regulatory elements and further revealing the transcriptional regulatory mechanisms. Chromatin profiling to characterize the combinatorial patterns of chromatin signatures is a powerful means to detect functional elements and clarify regulatory activities in human studies. RESULTS: In the present study, through comprehensive analyses of the open chromatin, DNA methylome, seven major chromatin marks, and transcriptomic data generated for seedlings of allohexaploid wheat, we detected distinct chromatin architectural features surrounding various functional elements, including genes, promoters, enhancer-like elements, and transposons. Thousands of new genic regions and cis-regulatory elements are identified based on the combinatorial pattern of chromatin features. Roughly 1.5% of the genome encodes a subset of active regulatory elements, including promoters and enhancer-like elements, which are characterized by a high degree of chromatin openness and histone acetylation, an abundance of CpG islands, and low DNA methylation levels. A comparison across sub-genomes reveals that evolutionary selection on gene regulation is targeted at the sequence and chromatin feature levels. The divergent enrichment of cis-elements between enhancer-like sequences and promoters implies these functional elements are targeted by different transcription factors. CONCLUSIONS: We herein present a systematic epigenomic map for the annotation of cis-regulatory elements in the bread wheat genome, which provides new insights into the connections between chromatin modifications and cis-regulatory activities in allohexaploid wheat.


Asunto(s)
Ensamble y Desensamble de Cromatina , Metilación de ADN , Código de Histonas , Elementos Reguladores de la Transcripción , Triticum/genética , Evolución Biológica , Epigenómica , Genoma de Planta , Plantones/metabolismo , Triticum/metabolismo
17.
Plant Biotechnol J ; 17(9): 1823-1833, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-30811829

RESUMEN

Nitrogen (N) plays critical role in plant growth; manipulating N assimilation could be a target to increase grain yield and N use. Here, we show that ABRE-binding factor (ABF)-like leucine zipper transcription factor TabZIP60 mediates N use and growth in wheat. The expression of TabZIP60 is repressed when the N-deprived wheat plants is exposed to nitrate. Knock down of TabZIP60 through RNA interference (RNAi) increases NADH-dependent glutamate synthase (NADH-GOGAT) activity, lateral root branching, N uptake and spike number, and improves grain yield more than 25% under field conditions, while overexpression of TabZIP60-6D had the opposite effects. Further investigation shows TabZIP60 binds to ABRE-containing fragment in the promoter of TaNADH-GOGAT-3B and negatively regulates its expression. Genetic analysis reveals that TaNADH-GOGAT-3B overexpression overcomes the spike number and yield reduction caused by overexpressing TabZIP60-6D. As such, TabZIP60-mediated wheat growth and N use is associated with its negative regulation on TaNADH-GOGAT expression. These findings indicate that TabZIP60 and TaNADH-GOGAT interaction plays important roles in mediating N use and wheat growth, and provides valuable information for engineering N use efficiency and yield in wheat.


Asunto(s)
Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/genética , Nitrógeno/metabolismo , Proteínas de Plantas/genética , Factores de Transcripción/genética , Triticum/genética , Grano Comestible/crecimiento & desarrollo , Técnicas de Silenciamiento del Gen , Triticum/crecimiento & desarrollo
18.
Front Plant Sci ; 9: 1614, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30459796

RESUMEN

Phosphorus (P) efficiency includes both P acquisition efficiency (PAE) and internal P utilization efficiency (PUE). Despite substantial research, genotypic variation in PAE and PUE remains incompletely understood in the field. A 2-year field study was conducted to compare PAE and PUE and related morphological, physiological, and molecular root traits of two winter wheat cultivars (Triticum aestivum L. cv. SJZ8 and KN92) in response to six P application rates in a P-deficient calcareous soil. Both cultivars showed similar growth and yield potential at each P supply level, reaching optimal growth at the same P application rate of about 100 kg P ha-1. However, the two cultivars differed in how they achieved yield and P efficiency. As P supply increased for both cultivars, root dry weight (RDW), root length density, and expression of the phosphate transporter gene TaPHT1.2 in roots initially increased and then stabilized, but arbuscular mycorrhizal fungal colonization, rhizosphere acid phosphatase activity, expressions of the P-starvation marker gene TaIPS1.1 and the purple acid phosphatase gene TaPAP16 in roots initially decreased and then stabilized. To enhance P acquisition when the P supply was deficient, KN92 modified the morphology of its roots, while SJZ8 increased the physiological activities in its roots. With an adequate P supply, high expression of TaPHT1.2 in roots might account for efficient P uptake for both cultivars, especially for KN92. Although P uptake per RDW was similar for both cultivars at anthesis, PAE was higher for KN92 than SJZ8 in terms of total P uptake in aboveground parts, whereas shoot and grain PUE were higher in SJZ8 than in KN92, mainly during the reproductive growth stage. These results indicate that P efficiency is under genotypic control at all P supply levels tested in both wheat cultivars, and that the two cultivars depend on different root strategies for P acquisition and utilization in response to changes in the P supply.

19.
Nucleic Acids Res ; 46(18): e107, 2018 10 12.
Artículo en Inglés | MEDLINE | ID: mdl-29931324

RESUMEN

Genetic diversity in plants is remarkably high. Recent whole genome sequencing (WGS) of 67 rice accessions recovered 10,872 novel genes. Comparison of the genetic architecture among divergent populations or between crops and wild relatives is essential for obtaining functional components determining crucial traits. However, many major crops have gigabase-scale genomes, which are not well-suited to WGS. Existing cost-effective sequencing approaches including re-sequencing, exome-sequencing and restriction enzyme-based methods all have difficulty in obtaining long novel genomic sequences from highly divergent population with large genome size. The present study presented a reference-independent core genome targeted sequencing approach, CGT-seq, which employed epigenomic information from both active and repressive epigenetic marks to guide the assembly of the core genome mainly composed of promoter and intragenic regions. This method was relatively easily implemented, and displayed high sensitivity and specificity for capturing the core genome of bread wheat. 95% intragenic and 89% promoter region from wheat were covered by CGT-seq read. We further demonstrated in rice that CGT-seq captured hundreds of novel genes and regulatory sequences from a previously unsequenced ecotype. Together, with specific enrichment and sequencing of regions within and nearby genes, CGT-seq is a time- and resource-effective approach to profiling functionally relevant regions in sequenced and non-sequenced populations with large genomes.


Asunto(s)
Epigénesis Genética/fisiología , Epigenómica/métodos , Especiación Genética , Variación Genética/genética , Tamaño del Genoma/fisiología , Secuenciación Completa del Genoma/métodos , Biología Computacional/métodos , Genoma/genética , Técnicas de Genotipaje/métodos , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Anotación de Secuencia Molecular/métodos , Oryza/clasificación , Oryza/genética , Análisis de Secuencia de ADN/métodos , Transcriptoma , Triticum/clasificación , Triticum/genética
20.
Planta ; 247(5): 1099-1108, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29356894

RESUMEN

MAIN CONCLUSION: Based on SLAF-seq, 67 Thinopyrum ponticum-specific markers and eight Th. ponticum-specific FISH probes were developed, and these markers and probes could be used for detection of alien chromatin in a wheat background. Decaploid Thinopyrum ponticum (2n = 10x = 70) is a valuable gene reservoir for wheat improvement. Identification of Th. ponticum introgression would facilitate its transfer into diverse wheat genetic backgrounds and its practical utilization in wheat improvement. Based on specific-locus-amplified fragment sequencing (SLAF-seq) technology, 67 new Th. ponticum-specific molecular markers and eight Th. ponticum-specific fluorescence in situ hybridization (FISH) probes have been developed from a tiny wheat-Th. ponticum translocation line. These newly developed molecular markers allowed the detection of Th. ponticum DNA in a variety of materials specifically and steadily at high throughput. According to the hybridization signal pattern, the eight Th. ponticum-specific probes could be divided into two groups. The first group including five dispersed repetitive sequence probes could identify Th. ponticum chromatin more sensitively and accurately than genomic in situ hybridization (GISH). Whereas the second group having three tandem repetitive sequence probes enabled the discrimination of Th. ponticum chromosomes together with another clone pAs1 in wheat-Th. ponticum partial amphiploid Xiaoyan 68.


Asunto(s)
Poaceae/genética , Cromatina/genética , ADN de Plantas/genética , Marcadores Genéticos/genética , Hibridación Fluorescente in Situ , Técnicas de Amplificación de Ácido Nucleico/métodos , Análisis de Secuencia de ADN/métodos , Triticum/genética
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