Understanding the interactions of genotype with environment and management (G×E×M) to maize productivity in Conservation Agriculture systems of Malawi.

Climatic variability and soil fertility decline present a fundamental challenge for smallholder farmers to determine the optimum management practices in the production of maize. Optimizing genotype (G) and management (M) of maize under different environmental conditions (E) and their interactions are essential for enhancing maize productivity in the smallholder sector of Malawi where maize is the main staple food. Here, we evaluated over seven seasons, the performance of four commercial maize genotypes [including hybrids and one open pollinated variety (OPV)] managed under different Conservation Agriculture (CA) and conventional practices (CP) across on-farm communities of central and southern Malawi. Our results revealed significant G×E and E×M interactions and showed that hybrids such as DKC 80-53 and PAN 53 outyielded the other hybrid and the OPV in most of the environments while the OPV ZM523 had greater yields in environments with above-average rainfall and shorter in-season dry spells. These environments received a maximum of 1250 mm to 1500 mm of rainfall and yet the long-term averages were 855 mm and 1248 mm, respectively. Despite yielding lower, the OPV ZM523 also exhibited higher yield stability across environments compared to the hybrid MH 30, possibly due to its resilience to drought, heat stress, and low soil fertility conditions which are often prevalent in the target communities. Conservation Agriculture-based practices outyielded CP across the genotypes and environments. However, amongst the CA-based systems, intercropping of maize with pigeonpea [Cajanus cajan (L.) Millsp] and cowpea (Vigna unguiculata Walp.) performed less than monocropping maize and then rotating it with a legume probably due to competition for moisture between the main and the companion crops in the intercrop. The key findings of this study suggest the need to optimize varietal and management options for particular environments to maximize maize productivity in Malawi. This means that smallholder farmers in Malawi should adopt hybrids and CA-based systems for enhanced yields but could also consider OPVs where the climate is highly variable. Further rigorous analysis that includes more abiotic stress factors is recommended for a better understanding of yield response.

Risk aversion, impatience, and adoption of conservation agriculture practices among smallholders in Zambia.

Sustainable agricultural practices such as conservation agriculture have been promoted in southern Africa for nearly three decades, but their adoption remains low. It is of policy interest to unpack behavioural drivers of adoption to understand why adoption remains lower than anticipated. This paper assesses the effects of risk aversion and impatience on the extent and intensity of the adoption of conservation agriculture using panel data collected from 646 households in 2021 and 2022 in Zambia. We find that 12% and 18% of the smallholders were impatient and risk averse, respectively. There are two main empirical findings based on panel data Probit and Tobit models. First, on the extensive margin, being impatient is correlated with a decreased likelihood of adopting combined minimum-tillage (MT) and rotation by 2.9 percentage points and being risk averse is associated with a decreased propensity of adopting combined minimum tillage (MT) and mulching by 3.2 percentage points. Being risk averse is correlated with a decreased chance of adopting basins by 2.8 percentage points. Second, on the intensive margin, impatience and risk aversion are significantly correlated with reduced adoption intensity of basins, ripping, minimum tillage (MT), and combined MT and rotation by 0.02-0.22 ha. These findings imply a need to embed risk management (e.g., through crop yield insurance) in the scaling of sustainable agricultural practices to incentivise adoption. This can help to nudge initial adoption and to protect farmers from yield penalties that are common in experimentation stages.

Genotypic variation in maize (Zea mays) influences rates of soil organic matter mineralization and gross nitrification.

Agricultural management practices that increase soil organic matter (SOM), such as no-tillage (NT) with crop residue retention, together with crop varieties best able to source nutrients from SOM, may help reverse soil degradation and improve soil nutrient supply and uptake by plants in low-input environments of tropical and subtropical areas. Here, we screened germplasm representing genetic diversity within tropical maize breeding programmes in relation to shaping SOM mineralization. Then we assessed effects of contrasting genotypes on nitrification rates, and genotype-by-management history interactions on these rates. SOM-C mineralization and gross nitrification rates varied under different maize genotypes. Cumulative SOM-C mineralization increased with root diameter but decreased with increasing root length. Strong influences of management history and interaction of maize genotype-by-management history on nitrification were observed. Overall, nitrification rates were higher in NT soil with residue retention. We propose that there is potential to exploit genotypic variation in traits associated with SOM mineralization and nitrification within breeding programmes. Root diameter and length could be used as proxies for root-soil interactions driving these processes. Development of maize varieties with enhanced ability to mineralize SOM combined with NT and residue retention to build/replenish SOM could be key to sustainable production.

Intensifying cropping systems through doubled-up legumes in Eastern Zambia.

Declining soil fertility and negative impacts of climate effects threaten the food security of millions in Africa. Conservation Agriculture (CA) is a promising strategy to address these challenges. However, lack of viable economic entry points and short-term benefits for smallholders limit its adoption. Legume intensification can possibly increase the output per unit area, thus making the system more attractive. Rotations of maize with intensified legume systems were tested for three consecutive years under ridge and furrow (RF) tillage and CA to investigate: (a) increases in productivity of legumes and the subsequent maize crop; (b) changes in land equivalent ratios (LERs) and; (c) improved total system productivity. Results showed an increase in legume yields when growing two legumes simultaneously, leading to greater LERs (ranging between 1.13 and 1.29). However, there was only a significant season and not a main treatment effect as CA did not outperform RF in both phases of the rotation. Full populations of companion legumes improved overall system productivity, yielding 76.8 GJ ha-1 in a more conducive season while sole cropping of pigeonpea yielded only 4.4 GJ ha-1. We conclude that the doubled-up legumes systems have great potential to improve household food security when integrated into current smallholder farming.

Combining local knowledge and soil science for integrated soil health assessments in conservation agriculture systems.

The challenges of soil degradation and climate change have led to the emergence of Conservation Agriculture (CA) as a sustainable alternative to tillage-based agriculture systems. Despite the recognition of positive impacts on soil health, CA adoption in Africa has remained low. Previous soil health studies have mainly focused on 'scientific' measurements, without consideration of local knowledge, which influences how farmers interpret CA impacts and future land management decisions. This study, based in Malawi, aims to 1) combine local knowledge and conventional soil science approaches to develop a contextualised understanding of the impact of CA on soil health; and 2) understand how an integrated approach can contribute to explaining farmer decision-making on land management. Key farmers' indicators of soil health were crop performance, soil consistence, moisture content, erosion, colour, and structure. These local indicators were consistent with conventional soil health indicators. By combining farmers' observations with soil measurements, we observed that CA improved soil structure, moisture (Mwansambo 7.54%-38.15% lower for CP; Lemu 1.57%-47.39% lower for CP) and infiltration (Lemu CAM/CAML 0.15 cms-1, CP 0.09 cms-1; Mwansambo CP/CAM 0.14 cms-1, CAML 0.18 cms-1). In the conventional practice, farmers perceived ridges to redistribute nutrients, which corresponded with recorded higher exchangeable ammonium (Lemu CP 76.0 mgkg -1, CAM 49.4 mgkg -1, CAML 51.7 mgkg -1), nitrate/nitrite values (Mwansambo CP 200.7 mgkg -1, CAM 171.9 mgkg -1, CAML 103.3 mgkg -1). This perception contributes to the popularity of ridges, despite the higher yield measurements under CA (Mwansambo CP 3225 kgha-1, CAML 5067 kgha-1, CAM 5160 kgha-1; Lemu CP 2886 kgha-1, CAM 2872 kgha-1, CAML 3454 kgha-1 ). The perceived carbon benefits of residues and ridge preference has promoted burying residues in ridges. Integrated approaches contribute to more nuanced and localized perceptions about land management. We propose that the stepwise integrated soil assessment framework developed in this study can be applied more widely in understanding the role of soil health in farmer-decision making, providing a learning process for downscaling technologies and widening the evidence base on sustainable land management practices.

Conservation Agriculture Affects Grain and Nutrient Yields of Maize (Zea Mays L.) and Can Impact Food and Nutrition Security in Sub-Saharan Africa.

Maize is a major staple and plays an essential role in food and nutrition security in Sub-Saharan Africa (SSA). Conservation agriculture (CA), a climate-smart agriculture practise based on minimum soil disturbance, crop residue retention, and crop diversification, has been widely advocated but without extensive research on the impact it may have on maize nutrient composition, and food and nutrition security. This study assessed the grain yield, macro- and micronutrient mineral content, and nutrient yield of eight maize varieties grown in Malawi, and how these are affected by CA practises over two seasons. The minerals were analysed by inductively coupled plasma (ICP) coupled to optical emission spectroscopy (OES) and to mass spectroscopy (MS). Grain yield and Se content differed among the varieties, while C, N, Fe, K, Mg, Mn, P, and Zn were similar. The local variety Kanjerenjere showed lowest grain and nutrient yields. The open-pollinated varieties (OPVs) concentrated more minerals than the F1 hybrids, but the latter showed higher yields for both grain and nutrients. Typical consumption of the eight maize varieties could fully meet the protein and Mg dietary reference intake (DRIs) of Malawian children (1-3 years), as well as Mg and Mn needs of adult women (19-50 years), but their contribution to dietary requirements was low for Fe (39-41%) and K (13-21%). The trials showed that CA increased grain yield (1.2- to 1.8-fold) and Se content (1.1- to 1.7-fold), but that it had no effect on C, K, Mg, P, and Zn, and that N (1.1- to 1.2-fold), Mn (1.1- to 1.8-fold), and Fe (1.3- to 3.4-fold) were reduced. The high increase in grain yield under CA treatments resulted in increased yields of protein and Se, no effect on the yields of K, Mg, Mn, P, Zn, and reduced Fe yield. Conservation agriculture could contribute in reducing the risk of Se deficiency in Malawian women and children but exacerbates the risk of Fe deficiency. A combination of strategies will be needed to mitigate some of the foreseen effects of climate change on agriculture, and food and nutrition security, and improve nutrient intake.

Leaf versus whole-canopy remote sensing methodologies for crop monitoring under conservation agriculture: a case of study with maize in Zimbabwe.

Enhancing nitrogen fertilization efficiency for improving yield is a major challenge for smallholder farming systems. Rapid and cost-effective methodologies with the capability to assess the effects of fertilization are required to facilitate smallholder farm management. This study compares maize leaf and canopy-based approaches for assessing N fertilization performance under different tillage, residue coverage and top-dressing conditions in Zimbabwe. Among the measurements made on individual leaves, chlorophyll readings were the best indicators for both N content in leaves (R < 0.700) and grain yield (GY) (R < 0.800). Canopy indices reported even higher correlation coefficients when assessing GY, especially those based on the measurements of the vegetation density as the green area indices (R < 0.850). Canopy measurements from both ground and aerial platforms performed very similar, but indices assessed from the UAV performed best in capturing the most relevant information from the whole plot and correlations with GY and leaf N content were slightly higher. Leaf-based measurements demonstrated utility in monitoring N leaf content, though canopy measurements outperformed the leaf readings in assessing GY parameters, while providing the additional value derived from the affordability and easiness of using a pheno-pole system or the high-throughput capacities of the UAVs.

Impacts of conservation agriculture on soil structure and hydraulic properties of Malawian agricultural systems.

Sub-Saharan Africa (SSA) faces climate change and food insecurity challenges, which require action to create resilient farming systems. Conservation agriculture (CA) is widely promoted across SSA but the impacts on key soil physical properties and functions such as soil structure and hydraulic properties that govern water storage and transmission are not well understood. The aim of this study was to assess the impacts of long term (10-12 years) maize-based CA on soil hydraulic conductivity, water retention and pore size distribution. Root zone (0-30 cm depth) soil total porosity, pore size distribution, saturated hydraulic conductivity (Ksat) and plant available water capacity (PAWC) of conventional maize monocrop farming systems (CP) are compared with those of adjacent CA trials with either sole maize or maize intercrop/rotation with cowpea (Vigna unguiculata L.), pigeon pea (Cajanus cajan L.) or velvet bean (Mucuna pruriens L) in trial locations across central and southern Malawi. Results show that maize-based CA systems result in significant changes to soil hydraulic properties that correlate with improved soil structure. Results demonstrate increases of 5-15 % in total porosity, 0.06-0.22 cm/min in Ksat, 3-7 % in fine pores for water storage and 3-6 % in PAWC. Maize monocrop CA had similar effect on the hydraulic properties as the maize-legume associations. The values of Ksat for CA systems were within optimum levels (0.03-0.3 cm/min) whereas PAWC was below optimum (<20 %). There was no significant build-up in soil organic matter (OM) in the CA systems. The results lead to a recommendation that crop residue management should be more pro-actively pursued in CA guidance from agricultural extension staff to increase soil OM levels, increase yields and enhance climate resilience of sub-Saharan African farming systems.

Agro-ecological options for fall armyworm (Spodoptera frugiperda JE Smith) management: Providing low-cost, smallholder friendly solutions to an invasive pest.

Fall armyworm (FAW), a voracious agricultural pest native to North and South America, was first detected on the African continent in 2016 and has subsequently spread throughout the continent and across Asia. It has been predicted that FAW could cause up to $US13 billion per annum in crop losses throughout sub-Saharan Africa, thereby threatening the livelihoods of millions of poor farmers. In their haste to respond to FAW governments may promote indiscriminate use of chemical pesticides which, aside from human health and environmental risks, could undermine smallholder pest management strategies that depend to a large degree on natural enemies. Agro-ecological approaches offer culturally appropriate low-cost pest control strategies that can be readily integrated into existing efforts to improve smallholder incomes and resilience through sustainable intensification. Such approaches should therefore be promoted as a core component of integrated pest management (IPM) programmes for FAW in combination with crop breeding for pest resistance, classical biological control and selective use of safe pesticides. Nonetheless, the suitability of agro-ecological measures for reducing FAW densities and impact need to be carefully assessed across varied environmental and socio-economic conditions before they can be proposed for wide-scale implementation. To support this process, we review evidence for the efficacy of potential agro-ecological measures for controlling FAW and other pests, consider the associated risks, and draw attention to critical knowledge gaps. The evidence indicates that several measures can be adopted immediately. These include (i) sustainable soil fertility management, especially measures that maintain or restore soil organic carbon; (ii) intercropping with appropriately selected companion plants; and (iii) diversifying the farm environment through management of (semi)natural habitats at multiple spatial scales. Nevertheless, we recommend embedding trials into upscaling programmes so that the costs and benefits of these interventions may be determined across the diverse biophysical and socio-economic contexts that are found in the invaded range.

Phenotyping Conservation Agriculture Management Effects on Ground and Aerial Remote Sensing Assessments of Maize Hybrids Performance in Zimbabwe.

In the coming decades, Sub-Saharan Africa (SSA) faces challenges to sustainably increase food production while keeping pace with continued population growth. Conservation agriculture (CA) has been proposed to enhance soil health and productivity to respond to this situation. Maize is the main staple food in SSA. To increase maize yields, the selection of suitable genotypes and management practices for CA conditions has been explored using remote sensing tools. They may play a fundamental role towards overcoming the traditional limitations of data collection and processing in large scale phenotyping studies. We present the result of a study in which Red-Green-Blue (RGB) and multispectral indexes were evaluated for assessing maize performance under conventional ploughing (CP) and CA practices. Eight hybrids under different planting densities and tillage practices were tested. The measurements were conducted on seedlings at ground level (0.8 m) and from an unmanned aerial vehicle (UAV) platform (30 m), causing a platform proximity effect on the images resolution that did not have any negative impact on the performance of the indexes. Most of the calculated indexes (Green Area (GA) and Normalized Difference Vegetation Index (NDVI)) were significantly affected by tillage conditions increasing their values from CP to CA. Indexes derived from the RGB-images related to canopy greenness performed better at assessing yield differences, potentially due to the greater resolution of the RGB compared with the multispectral data, although this performance was more precise for CP than CA. The correlations of the multispectral indexes with yield were improved by applying a soil-mask derived from a NDVI threshold with the aim of corresponding pixels with vegetation. The results of this study highlight the applicability of remote sensing approaches based on RGB images to the assessment of crop performance and hybrid choice.

The Cu2O2 torture track for a real-life system: [Cu2(btmgp)2O2](2+) oxo and peroxo species in density functional calculations.

Density functional theory (DFT) calculations of the equilibrium geometry, vibrational modes, ionization energies, electron affinities, and optical response of [Cu2(btmgp)2(µ-O)2](2+) (oxo) and [Cu2(btmgp)2(µ-η(2):η(2)-O2)](2+) (peroxo) are presented. Comprehensive benchmarking shows that the description of the oxo-peroxo energetics is still a torture track for DFT, but finds the molecular geometry to be comparatively robust with respect to changes in the exchange-correlation functionals and basis sets. Pure functionals favor the oxo core found experimentally, whereas hybrid functionals shift the bias toward the peroxo core. Further stabilization of peroxo core results from relaxing the spin degrees of freedom using the broken-symmetry (BS) approach. Dispersion effects, conversely, tend to favor the oxo configuration. Triple-zeta basis sets are found to represent a sensible compromise between numerical accuracy and computational effort. Particular attention is paid to the modification of the electronic structure, optical transitions, and excited-state energies along the transition path between the oxo and peroxo species. The excited-state potential energy surface calculations indicate that two triplet states are involved in the transition that stabilize the BS solution. Charge decomposition and natural transition orbital analyses are used for obtaining microscopic insight into the molecular orbital interactions. Here, the crucial role of guanidine π-interactions is highlighted for the stabilization of the Cu2O2 core.

A highly accurate potential energy curve for the mercury dimer.

The potential energy curve of the electronic ground state of the mercury dimer based on CCSD(T) calculations at the complete basis set (CBS) limit, including corrections for the full triples DeltaT and explicit spin-orbit (SO) interactions at the CCSD(T) level of theory, is presented. In the far long-range part, the potential energy curve is complemented by symmetry-adapted perturbation theory calculations. Potential curves of an analytically simple, extended Lennard-Jones form are obtained from very accurate fits to the CBS/CCSD(T)+SO and CBS/CCSD(T)+SO+DeltaT data. The Hg(2) potential curves yield dissociation energies of D(e)=424/392 cm(-1) and equilibrium distances of r(e)=3.650/3.679 A at the CBS/CCSD(T)+SO and CBS/CCSD(T)+SO+DeltaT levels of theory, respectively. By including perturbative quadruple corrections in our coupled-cluster calculations and corrections from correlating the 4f-core, we arrive at a final dissociation energy of D(e)=405 cm(-1), in excellent agreement with the experimentally estimated value of 407 cm(-1) by Greif and Hensel. In addition, the rotational and vibrational spectroscopic constants as well as the second virial coefficient B(T) in dependence of the temperature T are calculated and validated against available experimental and theoretical data.

Kinetic and thermodynamic stability of the group 13 trihydrides.

The kinetic and thermodynamic stabilities of the group 13 hydrides EH(3) (E = B, Al, Ga, In, Tl, E113) are investigated by relativistic density functional and wave function based theories. The unimolecular decomposition of EH(3) --> EH + H(2) becomes energetically more favorable going down the Group 13 elements, with the H(2)-abstraction of InH(3), TlH(3), and (E113)H(3) (E113: element with nuclear charge 113) being exothermic. In accordance with the Hammond-Leffler postulate, the activation barrier for the dissociation process decreases accordingly going down the group 13 elements in the periodic table shifting to an early transition state, with activation energies ranging from 88.4 kcal/mol for BH(3) to 41.3 kcal/mol for TlH(3) and only 21.6 kcal/mol for (E113)H(3) at the scalar relativistic coupled cluster level of theory. For both TlH(3) and (E113)H(3) we investigated spin-orbit effects using Dirac-Hartree-Fock and second-order Møller-Plesset theory to account for electron correlation. For (E113)H, spin-orbit coupling results in a chemically inert closed 7p(1/2)-shell, thus reducing the stability of the higher oxidation state even further. We also investigated the known organothallium compound Tl(CH(3))(3), which is thermodynamically unstable similar to TlH(3), but kinetically very stable with an activation barrier of 57.1 kcal/mol.

Nuclear quadrupole moment of 139La from relativistic electronic structure calculations of the electric field gradients in LaF, LaCl, LaBr, and LaI.

Relativistic coupled cluster theory is used to determine accurate electric field gradients in order to provide a theoretical value for the nuclear quadrupole moment of (139)La. Here we used the diatomic lanthanum monohalides LaF, LaCl, LaBr, and LaI as accurate nuclear quadrupole coupling constants are available from rotational spectroscopy by Rubinoff et al. [J. Mol. Spectrosc. 218, 169 (2003)]. The resulting nuclear quadrupole moment for (139)La (0.200+/-0.006 barn) is in excellent agreement with earlier work using atomic hyperfine spectroscopy [0.20(1) barn].