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1.
Front Cell Neurosci ; 12: 159, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29970990

RESUMEN

Recent advances in single-cell technologies are paving the way to a comprehensive understanding of the cellular complexity in the brain. Protocols for single-cell transcriptomics combine a variety of sophisticated methods for the purpose of isolating the heavily interconnected and heterogeneous neuronal cell types in a relatively intact and healthy state. The emphasis of single-cell transcriptome studies has thus far been on comparing library generation and sequencing techniques that enable measurement of the minute amounts of starting material from a single cell. However, in order for data to be comparable, standardized cell isolation techniques are essential. Here, we analyzed and simplified methods for the different steps critically involved in single-cell isolation from brain. These include enzymatic digestion, tissue trituration, improved methods for efficient fluorescence-activated cell sorting in samples containing high degree of debris from the neuropil, and finally, highly region-specific cellular labeling compatible with use of stereotaxic coordinates. The methods are exemplified using medium spiny neurons (MSN) from dorsomedial striatum, a cell type that is clinically relevant for disorders of the basal ganglia, including psychiatric and neurodegenerative diseases. We present single-cell RNA sequencing (scRNA-Seq) data from D1 and D2 dopamine receptor expressing MSN subtypes. We illustrate the need for single-cell resolution by comparing to available population-based gene expression data of striatal MSN subtypes. Our findings contribute toward standardizing important steps of single-cell isolation from adult brain tissue to increase comparability of data. Furthermore, our data redefine the transcriptome of MSNs at unprecedented resolution by confirming established marker genes, resolving inconsistencies from previous gene expression studies, and identifying novel subtype-specific marker genes in this important cell type.

2.
Front Pharmacol ; 9: 243, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29686613

RESUMEN

The central adenosine system and adenosine receptors play a fundamental role in the modulation of dopaminergic neurotransmission. This is mostly achieved by the strategic co-localization of different adenosine and dopamine receptor subtypes in the two populations of striatal efferent neurons, striatonigral and striatopallidal, that give rise to the direct and indirect striatal efferent pathways, respectively. With optogenetic techniques it has been possible to dissect a differential role of the direct and indirect pathways in mediating "Go" responses upon exposure to reward-related stimuli and "NoGo" responses upon exposure to non-rewarded or aversive-related stimuli, respectively, which depends on their different connecting output structures and their differential expression of dopamine and adenosine receptor subtypes. The striatopallidal neuron selectively expresses dopamine D2 receptors (D2R) and adenosine A2A receptors (A2AR), and numerous experiments using multiple genetic and pharmacological in vitro, in situ and in vivo approaches, demonstrate they can form A2AR-D2R heteromers. It was initially assumed that different pharmacological interactions between dopamine and adenosine receptor ligands indicated the existence of different subpopulations of A2AR and D2R in the striatopallidal neuron. However, as elaborated in the present essay, most evidence now indicates that all interactions can be explained with a predominant population of striatal A2AR-D2R heteromers forming complexes with adenylyl cyclase subtype 5 (AC5). The A2AR-D2R heteromer has a tetrameric structure, with two homodimers, which allows not only multiple allosteric interactions between different orthosteric ligands, agonists, and antagonists, but also the canonical Gs-Gi antagonistic interaction at the level of AC5. We present a model of the function of the A2AR-D2R heterotetramer-AC5 complex, which acts as an integrative device of adenosine and dopamine signals that determine the excitability and gene expression of the striatopallidal neurons. The model can explain most behavioral effects of A2AR and D2R ligands, including the psychostimulant effects of caffeine. The model is also discussed in the context of different functional striatal compartments, mainly the dorsal and the ventral striatum. The current accumulated knowledge of the biochemical properties of the A2AR-D2R heterotetramer-AC5 complex offers new therapeutic possibilities for Parkinson's disease, schizophrenia, SUD and other neuropsychiatric disorders with dysfunction of dorsal or ventral striatopallidal neurons.

3.
Curr Protoc Pharmacol ; 75: 11.19.1-11.19.21, 2016 12 13.
Artículo en Inglés | MEDLINE | ID: mdl-27960028

RESUMEN

Optogenetics is a method that uses light to control cells in living tissue, typically neurons, that have been modified to express light-sensitive ion channels and pumps. The approach facilitates neuromodulation in brain preparations and freely moving animals with unmatched spatial and temporal resolution. This optogenetics overview describes the vast array of light-sensitive proteins available and the methods used to deliver them to tissue and modulate them with light. How these methods have so far enhanced our knowledge of fundamental neuroscience and psychiatric disease will be discussed as well as how they may contribute to drug discovery in the future. Finally, the potential rewards and risks of therapeutic gene transfer of optogenetic proteins in humans will be considered. © 2016 by John Wiley & Sons, Inc.


Asunto(s)
Encefalopatías/terapia , Descubrimiento de Drogas/métodos , Terapia Genética/métodos , Fenómenos Fisiológicos del Sistema Nervioso , Neuronas/fisiología , Optogenética , Animales , Encéfalo/fisiología , Humanos , Canales Iónicos/metabolismo , Canales Iónicos/fisiología , Luz , Vías Nerviosas , Neuronas/metabolismo , Neurotransmisores/metabolismo , Opsinas/genética , Opsinas/metabolismo , Optogenética/instrumentación , Optogenética/métodos
4.
Cell ; 165(7): 1776-1788, 2016 Jun 16.
Artículo en Inglés | MEDLINE | ID: mdl-27238022

RESUMEN

A major challenge in understanding the cellular diversity of the brain has been linking activity during behavior with standard cellular typology. For example, it has not been possible to determine whether principal neurons in prefrontal cortex active during distinct experiences represent separable cell types, and it is not known whether these differentially active cells exert distinct causal influences on behavior. Here, we develop quantitative hydrogel-based technologies to connect activity in cells reporting on behavioral experience with measures for both brain-wide wiring and molecular phenotype. We find that positive and negative-valence experiences in prefrontal cortex are represented by cell populations that differ in their causal impact on behavior, long-range wiring, and gene expression profiles, with the major discriminant being expression of the adaptation-linked gene NPAS4. These findings illuminate cellular logic of prefrontal cortex information processing and natural adaptive behavior and may point the way to cell-type-specific understanding and treatment of disease-associated states.


Asunto(s)
Conducta Animal , Mapeo Encefálico/métodos , Corteza Prefrontal/citología , Animales , Conducta Apetitiva , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Cocaína/administración & dosificación , Electrochoque , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Corteza Prefrontal/metabolismo
5.
Nature ; 493(7433): 537-541, 2013 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-23235822

RESUMEN

Major depression is characterized by diverse debilitating symptoms that include hopelessness and anhedonia. Dopamine neurons involved in reward and motivation are among many neural populations that have been hypothesized to be relevant, and certain antidepressant treatments, including medications and brain stimulation therapies, can influence the complex dopamine system. Until now it has not been possible to test this hypothesis directly, even in animal models, as existing therapeutic interventions are unable to specifically target dopamine neurons. Here we investigated directly the causal contributions of defined dopamine neurons to multidimensional depression-like phenotypes induced by chronic mild stress, by integrating behavioural, pharmacological, optogenetic and electrophysiological methods in freely moving rodents. We found that bidirectional control (inhibition or excitation) of specified midbrain dopamine neurons immediately and bidirectionally modulates (induces or relieves) multiple independent depression symptoms caused by chronic stress. By probing the circuit implementation of these effects, we observed that optogenetic recruitment of these dopamine neurons potently alters the neural encoding of depression-related behaviours in the downstream nucleus accumbens of freely moving rodents, suggesting that processes affecting depression symptoms may involve alterations in the neural encoding of action in limbic circuitry.


Asunto(s)
Depresión/fisiopatología , Neuronas Dopaminérgicas/metabolismo , Animales , Depresión/inducido químicamente , Dopamina/metabolismo , Neuronas Dopaminérgicas/efectos de los fármacos , Neuronas Dopaminérgicas/efectos de la radiación , Femenino , Masculino , Ratones , Modelos Neurológicos , Núcleo Accumbens/metabolismo , Optogenética , Fenotipo , Ratas , Ratas Long-Evans , Estrés Psicológico/fisiopatología , Factores de Tiempo , Área Tegmental Ventral/citología
6.
Nature ; 492(7429): 428-32, 2012 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-23160494

RESUMEN

The prefrontal cortex (PFC) is thought to participate in high-level control of the generation of behaviours (including the decision to execute actions); indeed, imaging and lesion studies in human beings have revealed that PFC dysfunction can lead to either impulsive states with increased tendency to initiate action, or to amotivational states characterized by symptoms such as reduced activity, hopelessness and depressed mood. Considering the opposite valence of these two phenotypes as well as the broad complexity of other tasks attributed to PFC, we sought to elucidate the PFC circuitry that favours effortful behavioural responses to challenging situations. Here we develop and use a quantitative method for the continuous assessment and control of active response to a behavioural challenge, synchronized with single-unit electrophysiology and optogenetics in freely moving rats. In recording from the medial PFC (mPFC), we observed that many neurons were not simply movement-related in their spike-firing patterns but instead were selectively modulated from moment to moment, according to the animal's decision to act in a challenging situation. Surprisingly, we next found that direct activation of principal neurons in the mPFC had no detectable causal effect on this behaviour. We tested whether this behaviour could be causally mediated by only a subclass of mPFC cells defined by specific downstream wiring. Indeed, by leveraging optogenetic projection-targeting to control cells with specific efferent wiring patterns, we found that selective activation of those mPFC cells projecting to the brainstem dorsal raphe nucleus (DRN), a serotonergic nucleus implicated in major depressive disorder, induced a profound, rapid and reversible effect on selection of the active behavioural state. These results may be of importance in understanding the neural circuitry underlying normal and pathological patterns of action selection and motivation in behaviour.


Asunto(s)
Conducta Animal/fisiología , Motivación/fisiología , Neuronas/fisiología , Corteza Prefrontal/fisiología , Núcleos del Rafe/fisiología , Natación/fisiología , Potenciales de Acción , Animales , Axones/fisiología , Depresión/psicología , Electrofisiología , Locomoción/fisiología , Masculino , Optogenética , Ratas , Ratas Long-Evans , Sinapsis/fisiología , Factores de Tiempo
7.
Nature ; 471(7338): 358-62, 2011 Mar 17.
Artículo en Inglés | MEDLINE | ID: mdl-21389985

RESUMEN

Anxiety--a sustained state of heightened apprehension in the absence of immediate threat--becomes severely debilitating in disease states. Anxiety disorders represent the most common of psychiatric diseases (28% lifetime prevalence) and contribute to the aetiology of major depression and substance abuse. Although it has been proposed that the amygdala, a brain region important for emotional processing, has a role in anxiety, the neural mechanisms that control anxiety remain unclear. Here we explore the neural circuits underlying anxiety-related behaviours by using optogenetics with two-photon microscopy, anxiety assays in freely moving mice, and electrophysiology. With the capability of optogenetics to control not only cell types but also specific connections between cells, we observed that temporally precise optogenetic stimulation of basolateral amygdala (BLA) terminals in the central nucleus of the amygdala (CeA)--achieved by viral transduction of the BLA with a codon-optimized channelrhodopsin followed by restricted illumination in the downstream CeA--exerted an acute, reversible anxiolytic effect. Conversely, selective optogenetic inhibition of the same projection with a third-generation halorhodopsin (eNpHR3.0) increased anxiety-related behaviours. Importantly, these effects were not observed with direct optogenetic control of BLA somata, possibly owing to recruitment of antagonistic downstream structures. Together, these results implicate specific BLA-CeA projections as critical circuit elements for acute anxiety control in the mammalian brain, and demonstrate the importance of optogenetically targeting defined projections, beyond simply targeting cell types, in the study of circuit function relevant to neuropsychiatric disease.


Asunto(s)
Amígdala del Cerebelo/fisiología , Ansiedad/fisiopatología , Amígdala del Cerebelo/citología , Amígdala del Cerebelo/efectos de la radiación , Animales , Trastornos de Ansiedad/fisiopatología , Halorrodopsinas/metabolismo , Luz , Ratones , Modelos Neurológicos , Vías Nerviosas/fisiología , Vías Nerviosas/efectos de la radiación , Neuronas/fisiología , Neuronas/efectos de la radiación , Estrés Fisiológico/fisiología , Sinapsis/fisiología , Sinapsis/efectos de la radiación
8.
Nat Med ; 16(10): 1161-5, 2010 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-20871612

RESUMEN

A drawback of electrical stimulation for muscle control is that large, fatigable motor units are preferentially recruited before smaller motor units by the lowest-intensity electrical cuff stimulation. This phenomenon limits therapeutic applications because it is precisely the opposite of the normal physiological (orderly) recruitment pattern; therefore, a mechanism to achieve orderly recruitment has been a long-sought goal in physiology, medicine and engineering. Here we demonstrate a technology for reliable orderly recruitment in vivo. We find that under optical control with microbial opsins, recruitment of motor units proceeds in the physiological recruitment sequence, as indicated by multiple independent measures of motor unit recruitment including conduction latency, contraction and relaxation times, stimulation threshold and fatigue. As a result, we observed enhanced performance and reduced fatigue in vivo. These findings point to an unanticipated new modality of neural control with broad implications for nervous system and neuromuscular physiology, disease research and therapeutic innovation.


Asunto(s)
Electromiografía/métodos , Reclutamiento Neurofisiológico/fisiología , Animales , Channelrhodopsins , Estimulación Eléctrica , Ratones , Ratones Endogámicos C57BL , Neuronas Motoras/fisiología , Contracción Muscular , Nervios Periféricos/fisiología , Tiempo de Reacción
9.
Cell ; 141(1): 154-165, 2010 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-20303157

RESUMEN

Optogenetic technologies employ light to control biological processes within targeted cells in vivo with high temporal precision. Here, we show that application of molecular trafficking principles can expand the optogenetic repertoire along several long-sought dimensions. Subcellular and transcellular trafficking strategies now permit (1) optical regulation at the far-red/infrared border and extension of optogenetic control across the entire visible spectrum, (2) increased potency of optical inhibition without increased light power requirement (nanoampere-scale chloride-mediated photocurrents that maintain the light sensitivity and reversible, step-like kinetic stability of earlier tools), and (3) generalizable strategies for targeting cells based not only on genetic identity, but also on morphology and tissue topology, to allow versatile targeting when promoters are not known or in genetically intractable organisms. Together, these results illustrate use of cell-biological principles to enable expansion of the versatile fast optogenetic technologies suitable for intact-systems biology and behavior.


Asunto(s)
Técnicas Genéticas , Luz , Animales , Células Cultivadas , Hipocampo/citología , Hipocampo/metabolismo , Humanos , Neuronas/metabolismo , Proteínas Opsoninas/genética , Proteínas Opsoninas/metabolismo , Ratas , Biología de Sistemas/métodos
10.
Nature ; 458(7241): 1025-9, 2009 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-19295515

RESUMEN

In the study of complex mammalian behaviours, technological limitations have prevented spatiotemporally precise control over intracellular signalling processes. Here we report the development of a versatile family of genetically encoded optical tools ('optoXRs') that leverage common structure-function relationships among G-protein-coupled receptors (GPCRs) to recruit and control, with high spatiotemporal precision, receptor-initiated biochemical signalling pathways. In particular, we have developed and characterized two optoXRs that selectively recruit distinct, targeted signalling pathways in response to light. The two optoXRs exerted opposing effects on spike firing in nucleus accumbens in vivo, and precisely timed optoXR photostimulation in nucleus accumbens by itself sufficed to drive conditioned place preference in freely moving mice. The optoXR approach allows testing of hypotheses regarding the causal impact of biochemical signalling in behaving mammals, in a targetable and temporally precise manner.


Asunto(s)
Ingeniería Genética , Espacio Intracelular/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Transducción de Señal , Animales , Bovinos , Línea Celular , Cricetinae , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Humanos , Espacio Intracelular/efectos de la radiación , Ratones , Núcleo Accumbens/citología , Núcleo Accumbens/fisiología , Núcleo Accumbens/efectos de la radiación , Receptores Adrenérgicos alfa 1/genética , Receptores Adrenérgicos alfa 1/metabolismo , Receptores Adrenérgicos beta 2/genética , Receptores Adrenérgicos beta 2/metabolismo , Receptores Acoplados a Proteínas G/genética , Proteínas Recombinantes de Fusión/genética , Recompensa , Rodopsina/genética , Rodopsina/metabolismo , Transducción de Señal/efectos de la radiación , Relación Estructura-Actividad , Factores de Tiempo
11.
Science ; 324(5925): 354-9, 2009 Apr 17.
Artículo en Inglés | MEDLINE | ID: mdl-19299587

RESUMEN

Deep brain stimulation (DBS) is a therapeutic option for intractable neurological and psychiatric disorders, including Parkinson's disease and major depression. Because of the heterogeneity of brain tissues where electrodes are placed, it has been challenging to elucidate the relevant target cell types or underlying mechanisms of DBS. We used optogenetics and solid-state optics to systematically drive or inhibit an array of distinct circuit elements in freely moving parkinsonian rodents and found that therapeutic effects within the subthalamic nucleus can be accounted for by direct selective stimulation of afferent axons projecting to this region. In addition to providing insight into DBS mechanisms, these results demonstrate an optical approach for dissection of disease circuitry and define the technological toolbox needed for systematic deconstruction of disease circuits by selectively controlling individual components.


Asunto(s)
Axones/fisiología , Estimulación Encefálica Profunda , Neuronas Aferentes/fisiología , Trastornos Parkinsonianos/fisiopatología , Núcleo Subtalámico/fisiopatología , Potenciales de Acción , Animales , Astrocitos/metabolismo , Tecnología de Fibra Óptica , Halorrodopsinas/metabolismo , Luz , Actividad Motora , Corteza Motora/patología , Corteza Motora/fisiopatología , Inhibición Neural , Óptica y Fotónica , Trastornos Parkinsonianos/patología , Trastornos Parkinsonianos/terapia , Ratas , Rodopsina/metabolismo , Núcleo Subtalámico/patología
12.
Brain Cell Biol ; 36(1-4): 129-39, 2008 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-18677566

RESUMEN

Temporally precise inhibition of distinct cell types in the intact nervous system has been enabled by the microbial halorhodopsin NpHR, a fast light-activated electrogenic Cl(-) pump. While neurons can be optically hyperpolarized and inhibited from firing action potentials at moderate NpHR expression levels, we have encountered challenges with pushing expression to extremely high levels, including apparent intracellular accumulations. We therefore sought to molecularly engineer NpHR to achieve strong expression without these cellular side effects. We found that high expression correlated with endoplasmic reticulum (ER) accumulation, and that under these conditions NpHR colocalized with ER proteins containing the KDEL ER retention sequence. We screened a number of different putative modulators of membrane trafficking and identified a combination of two motifs, an N-terminal signal peptide and a C-terminal ER export sequence, that markedly promoted membrane localization and ER export defined by confocal microscopy and whole-cell patch clamp. The modified NpHR displayed increased peak photocurrent in the absence of aggregations or toxicity, and potent optical inhibition was observed not only in vitro but also in vivo with thalamic single-unit recording. The new enhanced NpHR (eNpHR) allows safe, high-level expression in mammalian neurons, without toxicity and with augmented inhibitory function, in vitro and in vivo.


Asunto(s)
Retículo Endoplásmico/metabolismo , Halorrodopsinas/metabolismo , Microscopía Confocal/métodos , Neuronas/metabolismo , Potenciales de Acción/fisiología , Animales , Animales Recién Nacidos , Células Cultivadas , Electrofisiología/métodos , Halorrodopsinas/genética , Halorrodopsinas/fisiología , Hipocampo/citología , Ratones , Ratones Endogámicos C57BL , Microscopía Confocal/instrumentación , Neuronas/citología , Neuronas/fisiología , Técnicas de Placa-Clamp/métodos , Ratas , Ratas Sprague-Dawley
14.
J Neurosci ; 27(48): 13210-21, 2007 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-18045915

RESUMEN

Phosphorylation-dependent changes in AMPA receptor function have a crucial role in activity-dependent forms of synaptic plasticity such as long-term potentiation (LTP) and long-term depression (LTD). Although three previously identified phosphorylation sites in AMPA receptor glutamate receptor 1 (GluR1) subunits (S818, S831, and S845) appear to have important roles in LTP and LTD, little is known about the role of other putative phosphorylation sites in GluR1. Here, we describe the characterization of a recently identified phosphorylation site in GluR1 at threonine 840. The results of in vivo and in vitro phosphorylation assays suggest that T840 is not a substrate for protein kinases known to phosphorylate GluR1 at previously identified phosphorylation sites, such as protein kinase A, protein kinase C, and calcium/calmodulin-dependent kinase II. Instead, in vitro phosphorylation assays suggest that T840 is a substrate for p70S6 kinase. Although LTP-inducing patterns of synaptic stimulation had no effect on GluR1 phosphorylation at T840 in the hippocampal CA1 region, bath application of NMDA induced a strong, protein phosphatase 1- and/or 2A-mediated decrease in T840 phosphorylation. Moreover, GluR1 phosphorylation at T840 was transiently decreased by a chemical LTD induction protocol that induced a short-term depression of synaptic strength and persistently decreased by a chemical LTD induction protocol that induced a lasting depression of synaptic transmission. Together, our results show that GluR1 phosphorylation at T840 is regulated by NMDA receptor activation and suggest that decreases in GluR1 phosphorylation at T840 may have a role in LTD.


Asunto(s)
Receptores AMPA/metabolismo , Receptores de N-Metil-D-Aspartato/fisiología , Treonina/metabolismo , Agonistas Adrenérgicos beta/farmacología , Análisis de Varianza , Animales , Células Cultivadas , Colforsina/farmacología , Inhibidores Enzimáticos/farmacocinética , Inhibidores Enzimáticos/farmacología , Fármacos actuantes sobre Aminoácidos Excitadores/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Hipocampo/citología , Humanos , Técnicas In Vitro , Isoproterenol/farmacología , Potenciación a Largo Plazo/efectos de los fármacos , Potenciación a Largo Plazo/fisiología , Ratones , Ratones Endogámicos C57BL , Mutagénesis/fisiología , Neuronas/efectos de los fármacos , Neuronas/fisiología , Neuronas/efectos de la radiación , Análisis por Matrices de Proteínas/métodos , Transfección/métodos
15.
Curr Opin Neurobiol ; 16(3): 329-35, 2006 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-16690311

RESUMEN

The polarized morphology of neurons poses a particular challenge to intracellular signal transduction. Local signals generated at distal sites must be retrogradely transported to the nucleus to produce persistent changes in neuronal function. Such communication of signals between distal neuronal compartments and the nucleus occurs during axon guidance, synapse formation, synaptic plasticity and following neuronal injury. Recent studies have begun to delineate a role for the active nuclear import pathway in transporting signals from axons and dendrites to the nucleus. In this pathway, soluble cargo proteins are recognized by nuclear transport carriers, called importins, which mediate their translocation from the cytoplasm into the nucleus. In neurons, importins might serve an additional function by carrying signals from distal sites to the soma.


Asunto(s)
Sistema Nervioso Central/metabolismo , Carioferinas/metabolismo , Neuronas/metabolismo , Transporte Activo de Núcleo Celular/fisiología , Animales , Axones/metabolismo , Axones/ultraestructura , Núcleo Celular/metabolismo , Sistema Nervioso Central/citología , Dendritas/metabolismo , Dendritas/ultraestructura , Humanos , Neuronas/citología , Transporte de Proteínas/fisiología , Transducción de Señal/fisiología
16.
Neuron ; 44(6): 997-1009, 2004 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-15603742

RESUMEN

The requirement for transcription during long-lasting plasticity indicates that signals generated at the synapse must be transported to the nucleus. We have investigated whether the classical active nuclear import pathway mediates intracellular retrograde signal transport in Aplysia sensory neurons and rodent hippocampal neurons. We found that importins localize to distal neuronal processes, including synaptic compartments, where they are well positioned to mediate synapse to nucleus signaling. In Aplysia, stimuli known to produce long-lasting but not short-lasting facilitation triggered importin nuclear translocation. In hippocampal neurons, NMDA receptor activation but not depolarization induced importin nuclear translocation. We further showed that LTP-inducing stimuli recruited active nuclear import in hippocampal slices. Together with our finding that long-term facilitation of Aplysia sensory-motor synapses required active nuclear import, our results indicate that regulation of the active nuclear import pathway plays a critical role in transporting synaptically generated signals into the nucleus during learning-related forms of plasticity.


Asunto(s)
Aplysia/fisiología , Núcleo Celular/fisiología , Carioferinas/fisiología , Potenciación a Largo Plazo/fisiología , Plasticidad Neuronal/fisiología , Sinapsis/fisiología , alfa Carioferinas/fisiología , Transporte Activo de Núcleo Celular/efectos de los fármacos , Transporte Activo de Núcleo Celular/fisiología , Animales , Aplysia/metabolismo , Núcleo Celular/efectos de los fármacos , Células Cultivadas , Carioferinas/metabolismo , Potenciación a Largo Plazo/efectos de los fármacos , Ratones , Datos de Secuencia Molecular , Plasticidad Neuronal/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Serotonina/metabolismo , Serotonina/farmacología , Sinapsis/efectos de los fármacos , alfa Carioferinas/metabolismo
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