Sequence Analysis and Biochemical Characteristics of Two Non-specific Lipid Transfer Proteins from Tartary Buckwheat Seeds.

INTRODUCTION: Plant non-specific lipid transfer proteins (nsLTPs) play an important role in plant resistance to various stresses, and show potential applications in agriculture, industrial manufacturing, and medicine. In addition, as more and more nsLTPs are identified as allergens, nsLTPs have attracted interest due to their allergenicity. Two nsLTPs from Tartary buckwheat have been isolated and identified. There is a need to study their biochemical characteristics and allergenicity. OBJECTIVE: The study aims to investigate the biochemical characteristics of two nsLTPs from Tartary buckwheat seeds and evaluate their potential allergenicity. METHODS: Two nsLTPs derived from Tartary buckwheat, namely FtLTP1a and FtLTP1b, were produced by gene cloning, expression, and purification. Sequence analysis and biochemical characteristics of the proteins, including lipid binding ability, α-amylase inhibition activity, antifungal activity, and allergenic activity, were investigated. RESULTS: High-purity recombinant FtLTP1a and FtLTP1b were obtained. FtLTP1a and FtLTP1b exhibited similar lipid binding and antifungal properties. Only FtLTP1b showed weak inhibitory activity against α-amylase. CONCLUSION: FtLTP1b could specifically bind IgE in the serum allergic to buckwheat and cross-react with pollen (w6). FtLTP1b is a novel allergenic member of the lipid-transfer protein 1 family found in Tartary buckwheat.

Experimental Study of Emulative Precast Concrete Beam-to-Column Connections Locally Reinforced by U-Shaped UHPC Shells.

Precast beam-column connections act as vital elements of precast concrete frames. To enhance the resistance to the earthquake-induced damage and environment-induced deterioration of precast beam-column connections, an innovative precast concrete beam-to-column connection locally enhanced by prefabricated ultra-high-performance concrete (UHPC) shells was proposed. For studying the seismic behaviors of these novel connections and the influence caused by the prefabricated UHPC shell length, full-scale precast specimens were experimentally investigated using low-cyclic reversed loading tests. The obtained results were analyzed and discussed, including hysteresis curves, skeleton curves, strength and deformability, performance degradation, energy dissipation capacities, and plastic hinge length. The results reveal that the novel precast concrete beam-column connections with UHPC shells behaved satisfactorily under seismic loadings. The damage in the concrete near the lower part of the beam end is reduced by the prefabricated UHPC shells. The longer prefabricated UHPC shells were more useful for decreasing the damage to the precast concrete components and improved the structural performance. The precast specimen with 600-mm long UHPC shells can achieve a ductility of 4.87 and 4.0% higher strength than the monolithic reference specimen.

OBP14 (Odorant-Binding Protein) Sensing in Adelphocoris lineolatus Based on Peptide Nucleic Acid and Graphene Oxide.

OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.

Uhrf1 regulates H3K9me2 modification of mTOR to inhibit the effect of autophagy in myocardial ischemia-reperfusion injury.

The regulation of mTOR and the dimethylation of histone H3 on lysine 9 (H3K9me2) H3K9me2 by Uhrf1 and the mechanism of autophagy regulation in myocardial ischemia-reperfusion injury (MIRI) were studied in vivo and in vitro. An in vitro I/R injury model was established using the primary mouse cardiomyocytes treated with H2O2. Subsequent analysis by qRT-PCR, western blot, and immunofluorescence indicated that overexpression of Uhrf1 significantly inhibited apoptosis of the H2O2-treated cardiomyocytes, reduced expression of apoptosis factors caspase-3 and Bax, and increased expression of apoptosis inhibitory factor Bcl-2. Furthermore, Uhrf1 was found to increase cardiomyocyte proliferation and promote the expression of mTOR, while the four expression peaks of H3K9me2 on the mTOR gene were inhibited by overexpression of Uhrf1. The expression of autophagy factors LC3, Beclin-1, and p-mTOR in Uhrf1-overexpressed cardiomyocytes was dramatically increased, and P62 expression was dramatically decreased. When an H3K9me2 inhibitor was added to the Uhrf1-knockdown cardiomyocytes, the expression of mTOR was increased, the expression of LC3, Beclin-1, and p-mTOR was decreased, and P62 expression was significantly increased. In the present study, Uhrf1 exhibits a protective function in MIRI, reducing the apoptosis of cardiomyocytes while increasing their proliferation and viability.

Identification of N-benzothiazolyl-2-benzenesulfonamides as novel ABCA1 expression upregulators.

ATP binding cassette transporter A1 (ABCA1) is a critical transporter that mediates cellular cholesterol efflux from macrophages to apolipoprotein A-I (ApoA-I). Therefore, increasing the expression level of ABCA1 is anti-atherogenic and ABCA1 expression upregulators have become novel choices for atherosclerosis treatment. In this study, a series of N-benzothiazolyl-2-benzenesulfonamides, based on the structure of WY06 discovered in our laboratory, were designed and synthesized as novel ABCA1 expression upregulators. Based on an in vitro ABCA1 upregulatory cell model, ABCA1 upregulation of target compounds was evaluated. Compounds 6c, 6d, and 6i have good upregulated ABCA1 expression activities, with EC50 values of 0.97, 0.37, and 0.41 µM, respectively. A preliminary structure-activity relationship is summarized. Replacing the methoxy group on the benzothiazole moiety of WY06 with a fluorine or chlorine atom and exchanging the ester group with a cyano group resulted in more potent ABCA1 upregulating activity. Moreover, compound 6i increased ABCA1 mRNA and protein expression and significantly promoted cholesterol efflux in RAW264.7 cells. In conclusion, N-benzothiazolyl-2-benzenesulfonamides were identified as novel ABCA1 expression upregulators.

Probability of cancer in high-risk patients predicted by the protein-based lung cancer biomarker panel in China: LCBP study.

BACKGROUND: The authors built a model for lung cancer diagnosis previously based on the blood biomarkers progastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC), and cytokeratin 19 fragment (CYFRA21-1). In the current study, they examined whether modification of the model to include relevant clinical information, risk factors, and low-dose chest computed tomography screening would improve the performance of the biomarker panel in large cohorts of Chinese adults. METHODS: The current study was a large-scale multicenter study (ClinicalTrials.gov identifier NCT01928836) performed in a Chinese population. A total of 715 participants were enrolled from 5 regional centers in Beijing, Henan, Nanjing, Shanghai, and Chongqing between October 2012 and February 2014. Serum biomarkers ProGRP, CEA, SCC, and CYFRA21-1 were analyzed on the ARCHITECT i2000SR. Relevant clinical information was collected and used to develop a patient risk model and a nodule risk model. RESULTS: The resulting patient risk model had an area under the receiver operating characteristic (ROC) curve of 0.7037 in the training data set and 0.7190 in the validation data set. The resulting nodule risk model had an area under the ROC curve of 0.9151 in the training data set and 0.5836 in the validation data set. Moreover, the nodule risk model had a relatively higher area under the ROC curve (0.9151 vs 0.8360; P = 0.001) compared with the American College of Chest Physician model in patients with lung nodules. CONCLUSIONS: Both the patient risk model and the nodule risk model, developed for the early diagnosis of lung cancer, demonstrated excellent discrimination, allowing for the stratification of patients with different levels of lung cancer risk. These new models are applicable in high-risk Chinese populations. Cancer 2018;124:262-70. © 2017 American Cancer Society.

Prevalence and risk factors for attempted suicide in the elderly: a cross-sectional study in Shanghai, China.

BACKGROUND: Previous studies have thoroughly investigated the prevalence and risk factors for completed suicide. In marked contrast is the lack of a better understanding of attempted suicide in the elderly. The aim of this study was to estimate the prevalence of attempted suicide in the elderly and examine the associated factors. METHODS: Using a multi-stage cluster sampling approach, a cross-sectional survey of 8,399 elderly house-dwelling residents was conducted in Shanghai, China. RESULTS: The two-week prevalence of attempted suicide in the elderly was 0.75%. In the bivariate analysis, having no caregivers, depressive, anxiety, sad, fear, obsessive-compulsive and anger symptom, and lower scores on the Barthel Index of Activities of Daily Living and the Lawton Instrumental Activities of Daily Living Scale were significantly associated with an increased risk of attempted suicide in the elderly. In the multivariate analysis, sad and fear symptoms were significantly and independently associated with a higher risk of attempted suicide in the elderly. CONCLUSION: The two-week prevalence of attempted suicide in the elderly is relatively high when compared with the annualized or lifetime prevalence reported in China and foreign settings. Elderly individuals with certain mental symptoms should be targeted for suicide prevention and provided with timely mental health support.

The association of multimorbidity and disability in a community-based sample of elderly aged 80 or older in Shanghai, China.

BACKGROUND: Both multimorbidity and activities of daily living (ADL) disability and instrument activities of daily living (IADL) disability are common among elderly individuals. ADL/IADL disability may reduce individuals' capacities for independent living and quality of life. This study aimed to examine the association between multimorbidity and ADL/IADL disability. METHODS: A multi-stage cluster sample of 2058 residents aged 80 or older was investigated in Shanghai, China. Multimorbidity was defined as the simultaneous presence of two or more chronic diseases with ten common chronic conditions under consideration. Subjects who responded that they "need partial or full assistance" to any ADL/IADL items were defined as having ADL/IADL disability. We examined the association of multimorbidity with ADL/IADL disability, adjusted for socio-demographic characteristics by using logistic regression. RESULTS: Of respondents, 23.23 % had ADL disability, 37.90 % had IADL disability, and 49.17 % had multimorbidity. After adjusted socio-demographic characteristics, a graded association was showed between ADL disability and the quantity of chronic conditions: odds ratio (OR) for 1 condition, 1.53(95 % confidence interval [CI], 1.04-2.24); OR for 2 conditions, 2.06(95 % CI, 1.43-2.96); OR for 3 conditions, 3.23(95 % CI, 2.14-4.86); OR for 4 or more conditions, 5.61(95 % CI, 3.26-9.66). Similar associations were also observed between the quantity of chronic conditions and IADL disability. CONCLUSIONS: The quantity of chronic conditions had relatively strong association with both ADL and IADL disability. Initiating prevention of additional chronic conditions and interventions on clusters of diseases may decrease the potential risk of ADL/IADL disability. Additionally, more attention should been given to the older low-income women living with relatives/non-relatives with multimorbidity.

Does disability predict attempted suicide in the elderly? A community-based study of elderly residents in Shanghai, China.

OBJECTIVES: Disability, which has been proved to be associated with suicide and suicidal ideation, has received little attention in relation to suicidal attempts among the elderly. The aim of this study was to explore the influence of disability on attempted suicide within this demographic. METHOD: A multi-stage cluster sample of 8399 residents aged 60 or more was investigated from 15 communities in Shanghai, China. Disability was measured using the Lawton instrumental activities of daily living (IADL) scale. RESULTS: The prevalence of attempted suicide in the elderly was 0.75%. Specific IADL disabilities, including shopping (OR = 3.01, 95% CI = 1.56-5.81), preparing meals (OR = 4.12, 95% CI = 2.12-8.00), housekeeping (OR = 2.48, 95% CI = 1.01-6.06), doing laundry (OR = 2.82, 95% CI = 1.09-7.35), using transport (OR = 3.10, 95% CI = 1.36-6.99) and medical care (OR = 4.41, 95% CI = 2.10-9.17), were significantly and independently associated with attempted suicide in the elderly. The presence of at least one such disability was associated with an almost threefold increase in the attempted suicide rate, and the presence of five or more IADL disabilities was associated with an approximate fivefold increase in the attempted suicide rate. CONCLUSION: Specific IADL disabilities, such as preparing meals or dealing with medical care, may be significant predictive factors for risk of suicidal attempts among the elderly. Therefore, elderly people with certain disabilities should be considered for suicide prevention interventions and should be supported in IADL as much as possible.

A Cross-sectional Study on Posttraumatic Stress Disorder and General Psychiatric Morbidity Among Adult Survivors 3 Years After the Wenchuan Earthquake, China.

After the Wenchuan earthquake, a large number of studies have focused on postearthquake psychological disorders among survivors; however, most of these studies were conducted within a relatively short period. This study was conducted to examine the symptoms of posttraumatic stress disorder (PTSD) and general psychiatric morbidity among adult survivors 3 years after the Wenchuan earthquake, China. Through a multistage systematic sampling approach, a cross-sectional survey of 360 participants, 18 years or older, was conducted. The prevalence of PTSD and general psychiatric morbidity was 10.3% and 20.6%, respectively. Multivariate analysis revealed significant predictors for PTSD, including female gender and having felt guilt concerning someone's death or injury. Significant predictors for general psychiatric morbidity included unmarried status and having been in serious danger. These results suggest that mental health services should be continuously available to earthquake survivors.

Comparative quality of life study between endoscopic sphincterotomy and surgical choledochotomy.

AIM: To determine quality of life improvement in choledocholithiasis patients who underwent endoscopic sphincterotomy (EST) versus open choledochotomy (OCT). METHODS: Eligible choledocholithiasis patients (n = 216) hospitalized in the Changhai Hospital between May 2010 and January 2011 were enrolled into a prospective study using cluster sampling. Patients underwent EST (n = 135) or OCT (n = 81) depending on the patient's wishes. Patients were followed-up with a field survey and by correspondence. Patients were also given the self-administered Gastrointestinal Quality of Life Index (GIQLI) to measure patient quality of life before surgery, and at two and six weeks after the procedures. RESULTS: With respect to baseline patient characteristics, the EST and OCT groups were comparable. After the procedure, gallstones were completely eliminated in all patients. Among 216 eligible patients, 191 patients (88.4%) completed all three surveys, including 118 patients who underwent EST (118/135; 87.4%) and 73 patients who underwent OCT (73/81; 90.1%). EST was associated with a significantly shorter hospital stay than OCT (8.8 ± 6.5 vs 13.9 ± 6.7 d; P < 0.001). The GIQLI score was similar between the EST and OCT groups before cholelithotomy (103.0 ± 15.4 vs 99.7 ± 10.2), but increased significantly in the EST group at two weeks (113.4 ± 12.0 vs 107.2 ± 11.2; P < 0.001) and six weeks (120.7 ± 10.6 vs 116.9 ± 7.5; P < 0.05) after the procedures. CONCLUSION: EST, compared with OCT, is associated with better postoperative quality of life in patients treated for choledocholithiasis.

Traumatic experiences and mental health consequences among child survivors of the 2008 Sichuan earthquake: a community-based follow-up study.

BACKGROUND: The study was implemented to examine the relationship between traumatic experiences and longitudinal development of mental health for children and adolescents who survived the 2008 Sichuan earthquake. METHODS: Using the method of multistage systematic sampling, 596 children aged between 8 and 16 years were randomly selected from severely affected areas of the earthquake. These children were interviewed with standardized instruments of posttraumatic stress disorder (PTSD) and depression at the 15th month after the earthquake, and re-interviewed at the 36th month. RESULTS: From the initial to the follow-up assessments, there were no significant changes in both PTSD and depression scores. In addition, no significant change was found on the overall prevalence rates of the symptoms: from 12.4% to 10.7% for PTSD, from 13.9% to 13.5% for depression, and from 4.2% to 4.7% for their co-occurrence. The study also indicated that the earthquake might have a delayed impact on the psychosocial functioning of children and adolescents who were not directly affected by the disaster. CONCLUSIONS: For child and adolescent survivors of the earthquake, symptoms of PTSD and depression seemed to persist over time. The finding that children reduced their use of mental health services raised great concerns over how to fulfill the unmet psychological needs of these children. More mental health interventions should be allocated to children who had elevated risk for developing persistent course of the symptoms.

Modelling medical care usage under medical insurance scheme for urban non-working residents.

This research investigates and evaluates China's urban medical care usage for non-working residents using microsimulation techniques. It focuses on modelling medical services usage and simulating medical expenses on hospitalization treatments as well as clinic services for serious illness in an urban area for the period of 2008-2010. A static microsimulation model was created to project the impact of the medical insurance scheme. Four kinds of achievements have been made. For three different scenarios, the model predicted the hospitalization services costs and payments, as well as the balance of the social pool fund and the medical burden on families.

Longitudinal study on health-related quality of life among child and adolescent survivors of the 2008 Sichuan earthquake.

PURPOSE: To longitudinally characterize child survivors' quality of life after a massive earthquake in low- and middle-income settings. METHODS: Population-based surveys were conducted in the severely affected areas 15 and 36 months after the earthquake, using a multi-stage systematic sampling design. RESULTS: A total of 596 participants were included in the initial assessment, of which 430 were re-surveyed in the follow-up assessment. For both assessments, means of the PedsQL total and subscale scores fell significantly below the general healthy children (P < 0.05 for all comparisons). Reduction in PedsQL total scores was observed from the initial to the follow-up assessment (82.2 vs. 80.3, P = 0.01). In regression analysis, mental health symptoms were examined as the biggest contributors for PedsQL scores, and girls and older children were found to report lower PedsQL scores than their counterparts. CONCLUSIONS: Health-related quality of life among child and adolescent survivors decreased over time. Besides helping children with identified risk experiences, attention should also be allocated to children without specific traumatic experiences, since the earthquake may have a delayed effect on them.

Mental health and quality of life survey among child survivors of the 2008 Sichuan earthquake.

PURPOSE: To characterize child survivors' mental health and quality of life after a massive earthquake. METHODS: A population-based survey of 596 children aged between 8 and 16 years was conducted in the severely affected areas of the earthquake, using a multi-stage systematic sampling design. RESULTS: The point prevalence rates of posttraumatic stress disorder (PTSD) and depression among the child survivors aged between 8 and 16 years 15 months after the earthquake were 12.4 and 13.9%, respectively. Health-related quality of life (HRQoL) was found to be strongly and negatively associated with PTSD and depression. Children who had lost family members were most likely to develop PTSD (OR = 6.6; P < 0.001) as well as depression (OR = 4.1; P < 0.001). Children who reported no utilization of mental health services were four times more likely to suffer from PTSD than those who did not (P = 0.002). CONCLUSIONS: More than one in five child survivors were identified as having PTSD or depression; HRQoL of these identified children were deeply compromised. Utilization of mental health services may significantly reduce the risk of developing PTSD symptoms.

Loneliness of left-behind children: a cross-sectional survey in a sample of rural China.

BACKGROUND: It is reported that about 28.3% of rural children estimated at 58 million were left behind in China when their parents migrated to cities to find work. These children may encounter critical situations of psychological well-being. We conducted a cross-sectional study to explore whether left-behind children are lonelier than the non-left-behind and to highlight subpopulations that are particular at risk. METHODS: We conducted a community-based cross-sectional study on a representative sample in rural China. Semi-structured questionnaires were applied to 324 left-behind and 282 non-left-behind children. Logistic regression analysis was used to find out risk factors associated with loneliness. RESULTS: We found that whether children were left behind or not influenced their likelihood of being lonely. Compared with non-left-behind children, the left-behind were 2.5 times (95% CI 1.7, 3.5) more likely to suffer from loneliness and 6.4 times (95% CI 4.2, 9.7) more likely to be very lonely. Left-behind children who were brought up by grandparents, or having poor economic status, bad relationship and low frequency of communication with parents were prone to encounter more as well as more severe loneliness. In particular, children who had a bad relationship with parents were at highest risk for severe loneliness (OR 14.5; 95% CI 2.1, 99.5). CONCLUSIONS: Left-behind children are at significant risk for loneliness. More specific investigations targeted towards the psychological well-being of these children are needed to identify the underlying preventable risk factors.

Are the elderly more vulnerable to psychological impact of natural disaster? A population-based survey of adult survivors of the 2008 Sichuan earthquake.

BACKGROUND: The association between ages and psychological impact of natural disasters has not been well characterized. A population-based study was conducted 15 months after the 2008 Sichuan earthquake to assess whether elderly survivors were more likely to develop posttraumatic stress disorder (PTSD) and general psychiatric morbidity. METHODS: A population-based survey of 327 survivors (152 elders, 175 younger adults) was conducted in severely affected areas by the earthquake, using a multi-stage systematic sampling design. RESULTS: Compared with the younger adult survivors, the elderly were more likely to have symptoms of PTSD (22.5% vs. 8.0%, p = 0.001) and general psychiatric morbidity (42.0% vs. 25.4%, p = 0.003). Risk factors, such as being elderly, having been in serious danger, having lost family members, and having felt guilt concerning one's death or injury were significantly associated with developing PTSD; being elderly, having family members or friends seriously injured, and having felt guilt concerning one's death or injury were significantly associated with developing general psychiatric morbidity. Utilization of mental health services is strongly associated with the decreased risk for developing both of the symptoms. CONCLUSION: Compared with the younger adults, the elderly survivors were more likely to develop PTSD and general psychiatric morbidity. More mental health services should be distributed to the elderly and groups at particular risk, to ensure their smooth mental health reconstruction after the earthquake.

Health-related quality of life of "left-behind children": a cross-sectional survey in rural China.

PURPOSE: To estimate the prevalence of children in rural China without constant parental guardians (i.e., "left-behind"), to examine whether left-behind children were associated with lower health-related quality of life (HRQOL) than their counterparts ("non-left-behind"). METHODS: A stratified two-stage cluster survey was conducted among 640 children aged between 8 and 14 in a county of Shandong province. HRQOL was assessed in 606 participants using the Pediatric Quality of Life Inventory (PedsQL). RESULTS: The estimated prevalence of left-behind children in the area was estimated at 53.5% (324/606). The mean PedsQL total scores were lower in the left-behind children than the non-left-behind (84.1 vs. 88.4; P < 0.01), as were psychosocial summary, emotional functioning, social functioning and school performance scores, while mean physical subscale scores did not differ significantly (85.4 vs. 86.2; P = 0.31). As age, education level and economic status increased, HRQOL of the children was significantly improved. CONCLUSIONS: Left-behind children report poorer HRQOL than non-left-behind children due to psychosocial dysfunction. An assessment of such problems is essential to estimate the need of rural children, for the identification of those at particular risk for lower quality of life, and for planning and implementation of appropriate health interventions.

Using a zeolite medium biofilter to remove organic pollutant and ammonia simultaneously.

A pilot scale zeolite medium biological aerated filter(ZBAF) was designed and used to treat municipal wastewater. It showed that ZBAF could simultaneously remove chemical oxygen demand(COD), ammonia-N and turbidity to satisfied degree at a hydraulic retention time(HRT) of 0.95 h. Their average removal efficiencies were 73.9%, 88.4% and 96.2% with the corresponding average effluent concentrations of 43.4 mg/L, 3.5 mg/L and 3.7 NTU, respectively. These effluent items met with the water quality standard of the treated water reused for cooling water. The COD removal volumetric loading rate increased proportionally with its applied volumetric loading rate with its maximum of 7.1 kg/(m3 x d). Ammonia-N removal loading rate also increased proportionally with its applied loading rate at HRT of longer than 0.95 h and the feasible maximum removal loading rate was 0.9 kg/(m3 x d). The COD loading rate did not affect the ammonia-N removal efficiency significantly when it was lower than 5.5 kg/(m3 x d). ZBAF has good application prospect for its low cost and high removal efficiency in the future.

[Performance and its influencing factors of a zeolite biofilter for dilute domestic wastewater treatment].

To reuse dilute domestic wastewater in power plant region as the makeup of recirculating cooling water, a renovate reactor--zeolite media biological aerated filter (ZBAF) was developed. A pilot scale ZBAF of 0.2 m in diameter and 3 m in media height was tested to treat dilute domestic wastewater. The results showed that turbidity, BOD5, CODcr and NH4(+)-N in effluent was 3.2 NTU, 3.2 mg/L, 14.5 mg/L and 0.5 mg/L when the corresponding influent concentration was 59 NTU, 30 mg/L, 81 mg/L and 16 mg/L, respectively, under the conditions of t = 12 degrees C-17 degrees C, HRT = 1.4 h and gas/liquid = 4:1. The effluent quality met the water quality standards of the treated water reused for cooling water makeup. The removal rate of BOD5, COD and turbidity did not change much with the change of HRT and the ratio of gas to liquid, but NH4(+)-N removal rate changed greatly. The parameters along the depth of ZBAF showed that the biofilm phase was abundant in carbon-oxidation/nitrification(C/N) zone and simplex in nitrification (N) zone. Ciliate was plentiful in C/N zone. Biofilm in N zone was mainly consists of nitrifiers. The point that the number of ciliate decreased apparently was the boundary of the two zones.