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1.
Brain Struct Funct ; 223(2): 1035-1047, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29299690

RESUMEN

A loss-of-function polymorphism in the α5 nicotinic acetylcholine receptor (nAChR) subunit gene has been linked to both drug abuse and schizophrenia. The α5 nAChR subunit is strategically positioned in the prefrontal cortex (PFC), where a loss-of-function in this subunit may contribute to cognitive disruptions in both disorders. However, the specific contribution of α5 to PFC-dependent cognitive functions has yet to be illustrated. In the present studies, we used RNA interference to knockdown the α5 nAChR subunit in the PFC of adult rats. We provide evidence that through its contribution to cholinergic modulation of cholinergic modulation of neurons in the PFC, the α5 nAChR plays a specific role in the recovery of attention task performance following distraction. Our combined data reveal the potent ability of this subunit to modulate the PFC and cognitive functions controlled by this brain region that are impaired in disease.


Asunto(s)
Atención/fisiología , Corteza Prefrontal/metabolismo , Receptores Nicotínicos/metabolismo , Acetilcolina/farmacología , Animales , Células Cultivadas , Embrión de Mamíferos , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Técnicas In Vitro , Masculino , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Corteza Prefrontal/citología , Células Piramidales/efectos de los fármacos , Células Piramidales/fisiología , ARN Mensajero/metabolismo , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , Ratas , Ratas Sprague-Dawley , Análisis y Desempeño de Tareas , Transducción Genética
2.
Proc Natl Acad Sci U S A ; 114(50): 13260-13265, 2017 12 12.
Artículo en Inglés | MEDLINE | ID: mdl-29158415

RESUMEN

Many debilitating neuropsychiatric and neurodegenerative disorders are characterized by dopamine neurotransmitter dysregulation. Monitoring subsecond dopamine release accurately and for extended, clinically relevant timescales is a critical unmet need. Especially valuable has been the development of electrochemical fast-scan cyclic voltammetry implementing microsized carbon fiber probe implants to record fast millisecond changes in dopamine concentrations. Nevertheless, these well-established methods have only been applied in primates with acutely (few hours) implanted sensors. Neurochemical monitoring for long timescales is necessary to improve diagnostic and therapeutic procedures for a wide range of neurological disorders. Strategies for the chronic use of such sensors have recently been established successfully in rodents, but new infrastructures are needed to enable these strategies in primates. Here we report an integrated neurochemical recording platform for monitoring dopamine release from sensors chronically implanted in deep brain structures of nonhuman primates for over 100 days, together with results for behavior-related and stimulation-induced dopamine release. From these chronically implanted probes, we measured dopamine release from multiple sites in the striatum as induced by behavioral performance and reward-related stimuli, by direct stimulation, and by drug administration. We further developed algorithms to automate detection of dopamine. These algorithms could be used to track the effects of drugs on endogenous dopamine neurotransmission, as well as to evaluate the long-term performance of the chronically implanted sensors. Our chronic measurements demonstrate the feasibility of measuring subsecond dopamine release from deep brain circuits of awake, behaving primates in a longitudinally reproducible manner.


Asunto(s)
Encéfalo/metabolismo , Dopamina/metabolismo , Electroencefalografía/métodos , Monitorización Neurofisiológica/métodos , Animales , Encéfalo/fisiología , Electrodos Implantados , Electroencefalografía/instrumentación , Femenino , Macaca mulatta , Monitorización Neurofisiológica/instrumentación , Recompensa , Factores de Tiempo
3.
Psychopharmacology (Berl) ; 232(21-22): 4113-27, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25963563

RESUMEN

RATIONALE: Gestational day 17 methylazoxymethanol (MAM) treatment has been shown to reproduce, in rodents, some of the alterations in cortical and mesolimbic circuitries thought to contribute to schizophrenia. OBJECTIVE: We characterized the behavior of MAM animals in tasks dependent on these circuitries to see what behavioral aspects of schizophrenia the model captures. We then characterized the integrity of mesolimbic dopamine neurotransmission in a subset of animals used in the behavioral experiments. METHODS: MAM animals' capacity for working memory, attention, and resilience to distraction was tested with two different paradigms. Cue-reward learning and motivation were assayed with Pavlovian conditioned approach. Measurements of electrically stimulated phasic and tonic DA release in the nucleus accumbens with fast-scan cyclic voltammetry were obtained from the same animals used in the Pavlovian task. RESULTS: MAM animals' basic attentional capacities were intact. MAM animals took longer to acquire the working memory task, but once learned, performed at the same level as shams. MAM animals were also slower to develop a Pavlovian conditioned response, but otherwise no different from controls. These same animals showed alterations in terminal DA release that were unmasked by an amphetamine challenge. CONCLUSIONS: The predominant behavioral-cognitive feature of the MAM model is a learning impairment that is evident in acquisition of executive function tasks as well as basic Pavlovian associations. MAM animals also have dysregulated terminal DA release, and this may contribute to observed behavioral differences. The MAM model captures some functional impairments of schizophrenia, particularly those related to acquisition of goal-directed behavior.


Asunto(s)
Modelos Animales de Enfermedad , Dopamina/metabolismo , Función Ejecutiva/efectos de los fármacos , Acetato de Metilazoximetanol/análogos & derivados , Núcleo Accumbens/metabolismo , Esquizofrenia/inducido químicamente , Psicología del Esquizofrénico , Anfetamina/farmacología , Animales , Condicionamiento Clásico/efectos de los fármacos , Aprendizaje/efectos de los fármacos , Masculino , Motivación/efectos de los fármacos , Núcleo Accumbens/efectos de los fármacos , Ratas Sprague-Dawley , Recompensa , Esquizofrenia/fisiopatología
4.
Nature ; 500(7464): 575-9, 2013 Aug 29.
Artículo en Inglés | MEDLINE | ID: mdl-23913271

RESUMEN

Predictions about future rewarding events have a powerful influence on behaviour. The phasic spike activity of dopamine-containing neurons, and corresponding dopamine transients in the striatum, are thought to underlie these predictions, encoding positive and negative reward prediction errors. However, many behaviours are directed towards distant goals, for which transient signals may fail to provide sustained drive. Here we report an extended mode of reward-predictive dopamine signalling in the striatum that emerged as rats moved towards distant goals. These dopamine signals, which were detected with fast-scan cyclic voltammetry (FSCV), gradually increased or--in rare instances--decreased as the animals navigated mazes to reach remote rewards, rather than having phasic or steady tonic profiles. These dopamine increases (ramps) scaled flexibly with both the distance and size of the rewards. During learning, these dopamine signals showed spatial preferences for goals in different locations and readily changed in magnitude to reflect changing values of the distant rewards. Such prolonged dopamine signalling could provide sustained motivational drive, a control mechanism that may be important for normal behaviour and that can be impaired in a range of neurologic and neuropsychiatric disorders.


Asunto(s)
Dopamina/metabolismo , Neostriado/metabolismo , Recompensa , Transducción de Señal , Potenciales de Acción , Animales , Neuronas Dopaminérgicas/metabolismo , Objetivos , Masculino , Aprendizaje por Laberinto , Modelos Neurológicos , Modelos Psicológicos , Motivación , Neostriado/citología , Ratas , Ratas Long-Evans , Factores de Tiempo
5.
J Neurophysiol ; 107(7): 1979-95, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22170970

RESUMEN

A major goal of neuroscience is to understand the functions of networks of neurons in cognition and behavior. Recent work has focused on implanting arrays of ∼100 immovable electrodes or smaller numbers of individually adjustable electrodes, designed to target a few cortical areas. We have developed a recording system that allows the independent movement of hundreds of electrodes chronically implanted in several cortical and subcortical structures. We have tested this system in macaque monkeys, recording simultaneously from up to 127 electrodes in 14 brain regions for up to one year at a time. A key advantage of the system is that it can be used to sample different combinations of sites over prolonged periods, generating multiple snapshots of network activity from a single implant. Used in conjunction with microstimulation and injection methods, this versatile system represents a powerful tool for studying neural network activity in the primate brain.


Asunto(s)
Potenciales de Acción/fisiología , Encéfalo/citología , Electrodos Implantados , Microelectrodos , Movimiento , Neuronas/fisiología , Animales , Diseño Asistido por Computadora , Macaca , Reproducibilidad de los Resultados , Factores de Tiempo
6.
Neuron ; 66(6): 921-36, 2010 Jun 24.
Artículo en Inglés | MEDLINE | ID: mdl-20620877

RESUMEN

To study the interplay between hippocampus and medial prefrontal cortex (Pfc) and its importance for learning and memory consolidation, we measured the coherence in theta oscillations between these two structures in rats learning new rules on a Y maze. Coherence peaked at the choice point, most strongly after task rule acquisition. Simultaneously, Pfc pyramidal neurons reorganized their phase, concentrating at hippocampal theta trough, and synchronous cell assemblies emerged. This synchronous state may result from increased inhibition exerted by interneurons on pyramidal cells, as measured by cross-correlation, and could be modulated by dopamine: we found similar hippocampal-Pfc theta coherence increases and neuronal phase shifts following local administration of dopamine in Pfc of anesthetized rats. Pfc cell assemblies emerging during high coherence were preferentially replayed during subsequent sleep, concurrent with hippocampal sharp waves. Thus, hippocampal/prefrontal coherence could lead to synchronization of reward predicting activity in prefrontal networks, tagging it for subsequent memory consolidation.


Asunto(s)
Potenciales de Acción/fisiología , Hipocampo/fisiología , Aprendizaje por Laberinto/fisiología , Neuronas/fisiología , Periodicidad , Corteza Prefrontal/fisiología , Potenciales de Acción/efectos de los fármacos , Animales , Conducta Animal , Dopamina/farmacología , Masculino , Inhibición Neural/efectos de los fármacos , Inhibición Neural/fisiología , Vías Nerviosas/fisiología , Neuronas/clasificación , Neuronas/efectos de los fármacos , Análisis de Componente Principal , Ratas , Ratas Long-Evans , Recompensa , Análisis Espectral/métodos , Factores de Tiempo
7.
Proc Natl Acad Sci U S A ; 106(33): 14114-9, 2009 Aug 18.
Artículo en Inglés | MEDLINE | ID: mdl-19666522

RESUMEN

In the weeks following unilateral peripheral nerve injury, the deprived primary somatosensory cortex (SI) responds to stimulation of the ipsilateral intact limb as demonstrated by functional magnetic resonance imaging (fMRI) responses. The neuronal basis of these responses was studied by using high-resolution fMRI, in vivo electrophysiological recordings, and juxtacellular neuronal labeling in rats that underwent an excision of the forepaw radial, median, and ulnar nerves. These nerves were exposed but not severed in control rats. Significant bilateral increases of fMRI responses in SI were observed in denervated rats. In the healthy SI of the denervated rats, increases in fMRI responses were concordant with increases in local field potential (LFP) amplitude and an increased incidence of single units responding compared with control rats. In contrast, in the deprived SI, increases in fMRI responses were associated with a minimal change in LFP amplitude but with increased incidence of single units responding. Based on action potential duration, juxtacellular labeling, and immunostaining results, neurons responding to intact forepaw stimulation in the deprived cortex were identified as interneurons. These results suggest that the increases in fMRI responses in the deprived cortex reflect increased interneuron activity.


Asunto(s)
Interneuronas/metabolismo , Imagen por Resonancia Magnética/métodos , Nervios Periféricos/patología , Algoritmos , Animales , Corteza Cerebral/patología , Biología Computacional , Electrofisiología/métodos , Modelos Biológicos , Modelos Neurológicos , Modelos Estadísticos , Neuronas/metabolismo , Nervios Periféricos/metabolismo , Ratas , Ratas Sprague-Dawley , Procesamiento de Señales Asistido por Computador
8.
Eur J Neurosci ; 20(2): 514-24, 2004 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-15233760

RESUMEN

The hippocampus and prefrontal cortex (PFC), two structures implicated in learning and memory processes, are linked by a direct hippocampo-prefrontal pathway. It has been shown that PFC pyramidal cells receive monosynaptic excitatory inputs from the hippocampus and, in this study, we sought to determine the influence of the hippocampus on PFC interneurons in anesthetized rats. Extracellular recordings were coupled to juxtacellular injections of neurobiotin or biotinylated dextran amine to morphologically differentiate interneurons from pyramidal cells. In all cases, the action potentials of labeled interneurons were of shorter duration (< 0.70 ms) than those of identified pyramidal cells (> 0.70 ms). Single pulse stimulation of the hippocampal CA1/subiculum region induced an excitatory response in 70% of recorded interneurons in the prelimbic and medial-orbital areas of the PFC. In contrast to the one to two action potentials generated by pyramidal cells, an important group of interneurons fired a burst of action potentials in response to hippocampal stimulation. A large proportion of these excitatory responses was probably monosynaptic as their latency is consistent with the conduction time of the hippocampo-prefrontal pathway. In addition, when both a pyramidal cell and an interneuron were simultaneously recorded and both responded to stimulation, the interneuron consistently fired before the pyramidal cell. In conclusion, the hippocampus exerts a direct excitatory influence on PFC interneurons and is thus capable of feedforward inhibition of pyramidal cells. Hippocampal output is spatially and temporally focalized via this inhibitory process and consequently could facilitate the synchronization of a specific subset of PFC neurons with hippocampal activity.


Asunto(s)
Hipocampo/fisiología , Interneuronas/fisiología , Corteza Prefrontal/citología , Potenciales de Acción/fisiología , Potenciales de Acción/efectos de la radiación , Animales , Recuento de Células/métodos , Estimulación Eléctrica/métodos , Hipocampo/citología , Interneuronas/clasificación , Masculino , Potenciales de la Membrana/fisiología , Potenciales de la Membrana/efectos de la radiación , Inhibición Neural/fisiología , Inhibición Neural/efectos de la radiación , Corteza Prefrontal/fisiología , Células Piramidales/fisiología , Células Piramidales/efectos de la radiación , Ratas , Ratas Sprague-Dawley , Factores de Tiempo
9.
J Neurophysiol ; 87(2): 1118-22, 2002 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-11826075

RESUMEN

Multisecond oscillations in firing rate with periods in the range of 2-60 s (mean, 20-35 s) are present in 50-90% of spike trains from basal ganglia neurons recorded from locally anesthetized, immobilized rats. To determine whether these periodic oscillations are associated with similar periodicities in cortical activity, transcortical electroencephalographic (EEG) activity was recorded in conjunction with single- or dual-unit neuronal activity in the subthalamic nucleus (STN) or the globus pallidus (GP), and the data were analyzed with spectral and wavelet analyses. Multisecond oscillations in firing rates of 31% of the STN neurons and 46% of the GP neurons with periodicities significantly correlated with bursts of theta (4-7 Hz) activity in transcortical EEG. Further recordings of localized field potentials in the hippocampus and frontal or parietal cortices simultaneously with GP unit activity showed field potentials from the hippocampus, but not from the frontal or parietal cortices, exhibited bursts of theta rhythm that were correlated with GP firing rate oscillations. These results demonstrate a functional connectivity between basal ganglia neuronal activity and theta band activity in the hippocampus.


Asunto(s)
Ganglios Basales/fisiología , Hipocampo/fisiología , Ritmo Teta , Animales , Lóbulo Frontal/fisiología , Masculino , Lóbulo Parietal/fisiología , Periodicidad , Ratas , Ratas Sprague-Dawley
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