RESUMEN
The rise of shear strain value under temperature increase in biological tissue samples in vitro and tissue phantoms was studied and the range of shear modulus and viscosity calculated. It has been shown that the acoustic radiation force-based methods with the usage of ultrasound Doppler probing provides the potential ability of noninvasive real-time monitoring of tissues' ultrasound thermal destruction process. At that, the thermal destruction is possible under action of wave beam that creates the radiation force and local tissue displacements so that tissue ablation and acoustic remote palpation could be realized by means of the same ultrasound transducer. The experiments were performed using gelatin-based tissue-mimicking phantoms and freshly excised samples of bovine muscle tissue. It was determined also that fluctuating pattern of detected displacement amplitude variation is the indicator of the phase transitions beginning in the heated field of soft tissue or tissue phantom.
Asunto(s)
Hipertermia Inducida/métodos , Terapia por Ultrasonido/métodos , Animales , Bovinos , Elasticidad , Humanos , Palpación , Fantasmas de Imagen , Estrés Mecánico , Ultrasonografía Doppler/métodos , ViscosidadRESUMEN
The shift of prooxidant-antioxidant balance in side of prooxidants was revealed in rat liver mitochondria and in microsomes and in blood plasma in response to single irradiation (dose 8 Gy). The shift was more expressed in animals with nutrition unbalanced on animal proteins and antioxidant vitamins. In the main it was explained by the initially reduced activity of enzymatic antioxidant system and especially Se-dependent glutathione peroxidase activity. The apply of food addition from Aronia melanocarpa fruits had delayed lipid peroxidation activation in irradiated animals but practically had no effect on activity of enzymatic antioxidant system. The established essential decrease of Se-dependent glutathione peroxidase activity under unbalanced diet is considered the most crucial point in the maintenance of enzymatic antioxidant system reliability in irradiated animals.
Asunto(s)
Antioxidantes/efectos de la radiación , Rayos gamma , Hígado/efectos de la radiación , Photinia , Traumatismos Experimentales por Radiación/metabolismo , Especies Reactivas de Oxígeno/efectos de la radiación , Animales , Antioxidantes/metabolismo , Catalasa/metabolismo , Glutatión Peroxidasa/sangre , Glutatión Peroxidasa/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Peroxidación de Lípido/efectos de la radiación , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Microsomas Hepáticos/metabolismo , Mitocondrias Hepáticas/metabolismo , Mitocondrias Hepáticas/efectos de la radiación , Oxidación-Reducción/efectos de la radiación , Preparaciones de Plantas/farmacología , Preparaciones de Plantas/uso terapéutico , Traumatismos Experimentales por Radiación/terapia , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismo , Selenio/metabolismo , Superóxido Dismutasa/sangre , Superóxido Dismutasa/metabolismo , Irradiación Corporal TotalRESUMEN
The role of the glutathione-dependent antioxidant system in the prooxidant-antioxidant balance support was studied in the experiments with Wistar male rat under single gamma-irradiation (8 Gr dose), fed with unbalanced (as to animal proteins and antioxidant vitamins) diet and with addition of Aronia melanocarpa. Single gamma-irradiation of animals led to the decrease of selenium-dependent glutathione-peroxidase activity in the blood plasma and glutathione-S-transferase activity decrease in rat liver mitochondria. Animals which received the unbalanced food allowance under irradiation showed more expressed change of glutathione-dependent antioxidant enzymes activity, namely--proved decrease of glutathione-peroxidase and glutathione-S-transferase activity in liver microsomes and less expressed activation of selenium-dependent glutathione-peroxidase activity in the postmitochondrial fraction of laboratory animals liver. Introduction of the A. melanocarpa food supplement in the unbalanced diet of the laboratory animals which in vitro demonstrated expressed antioxidant and antiradical activity had no effect upon glutathione-peroxidase activity in the investigated tissues. Obtained data concerning significant decrease of the activity of glutathione-dependent antioxidant system and, particularly, of the selenium-dependent glutathione-peroxidase activity under the unbalanced diet condition may be useful in maintenance of prooxidant-antioxidant balance in the tissues of irradiated animals. Allowing for the above stated it is advisable to seek for new food additives which increase activity of the endogenous glutathione-dependent antioxidant enzymes for human tolerance improvement, especially under the unbalanced food allowance condition.
Asunto(s)
Antioxidantes/metabolismo , Dieta , Glutatión Peroxidasa/sangre , Glutatión Transferasa/sangre , Hígado/efectos de la radiación , Animales , Suplementos Dietéticos , Rayos gamma , Glutatión Peroxidasa/metabolismo , Glutatión Transferasa/metabolismo , Hígado/enzimología , Hígado/metabolismo , Masculino , Microsomas Hepáticos/enzimología , Microsomas Hepáticos/metabolismo , Microsomas Hepáticos/efectos de la radiación , Mitocondrias Hepáticas/enzimología , Mitocondrias Hepáticas/metabolismo , Mitocondrias Hepáticas/efectos de la radiación , Photinia/química , Extractos Vegetales/administración & dosificación , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/uso terapéutico , Traumatismos Experimentales por Radiación/sangre , Traumatismos Experimentales por Radiación/metabolismo , Traumatismos Experimentales por Radiación/prevención & control , Ratas , Ratas WistarRESUMEN
Shear wave elasticity imaging (SWEI), an emerging acoustic technology for medical diagnostics, is based on remote generation of shear waves in tissue by radiation force in the focal region of an ultrasonic beam. In this study, the feasibility of Doppler ultrasonic technique to visualize the remotely induced shear waves was demonstrated. The generation of shear displacement in the focal region of a pulsed 1-MHz ultrasound beam with pulse duration of approximately about 2 ms and intensity levels on the order of 145 W/cm2, and consequent propagation of shear wave in tissue-mimicking and muscle tissue in vitro, were measured. The analysis of temporal behavior of shear displacement within the focal plane allowed estimation of shear wave velocities. The velocities were 4 and 7 m/s in hard phantom and tissue containing phantom, respectively. The measured shear displacements on the order of micrometers in gel-based phantoms are in reasonable agreement with theoretical estimates derived from an earlier developed model of shear wave generation by radiation force of focused ultrasound. The study revealed significant dependence of shear strain on the medium viscosity. The complex oscillatory character of shear strain relaxation in viscoelastic phantom and muscle tissue in vitro was observed.
Asunto(s)
Elasticidad , Gelatina/química , Glicerol/química , Músculos/fisiología , Ultrasonido , Animales , Bovinos , Humanos , Modelos Teóricos , Fantasmas de Imagen , ViscosidadRESUMEN
The effect of triton X-100 and glutaraldehyde on the changes in Ca(2+)- and Mg(2+)-ATPase activity of erythrocyte plasma membrane under irradiation of rat erythrocytes has been studied. It was found that irradiation of erythrocyte ghosts in a dose of 1000 Gy leads to the inhibition of the both enzymes. Triton X-100 enhanced the radiation effect, while glutaraldehyde decreased Ca(2+)-ATPase sensitivity to the action of radiation. The mechanisms of the effects observed are discussed.
Asunto(s)
ATPasa de Ca(2+) y Mg(2+)/efectos de los fármacos , ATPasas Transportadoras de Calcio/efectos de los fármacos , Membrana Eritrocítica/efectos de los fármacos , Glutaral/farmacología , Octoxinol/farmacología , Animales , ATPasa de Ca(2+) y Mg(2+)/sangre , ATPasas Transportadoras de Calcio/sangre , Detergentes , Membrana Eritrocítica/enzimología , Membrana Eritrocítica/efectos de la radiación , Membrana Eritrocítica/ultraestructura , RatasRESUMEN
The effect of triton X-100 and glutaraldehyde on the functioning of microsomal oxidoreductases has been investigated. Triton X-100 was found to increase the enzymes activity. The decrease of oxidoreductases activity was observed in the presence of glutaraldehyde.
Asunto(s)
Glutaral/farmacología , Membranas Intracelulares/efectos de los fármacos , Microsomas Hepáticos/efectos de los fármacos , Octoxinol/farmacología , Oxidorreductasas/efectos de los fármacos , Animales , Detergentes , Microsomas Hepáticos/enzimología , Microsomas Hepáticos/ultraestructura , ConejosRESUMEN
The effect of electrons with energy 5 MeV on the functional state of rabbit liver microsome oxidoreductases has been investigated. Decrease in enzyme activity upon irradiation with a dose of 10 kGy was found. The possible mechanisms of the effects observed are discussed.
Asunto(s)
Microsomas Hepáticos/enzimología , Oxidorreductasas/antagonistas & inhibidores , Animales , Relación Dosis-Respuesta en la Radiación , Técnicas In Vitro , Peroxidación de Lípido/efectos de la radiación , Malondialdehído/metabolismo , Microsomas Hepáticos/metabolismo , Microsomas Hepáticos/efectos de la radiación , Conejos , Tolerancia a RadiaciónRESUMEN
The effect of electrons with the energy of 5 MeV on the erythrocyte membrane structure was investigated using a fluorescent probe (4-dimethylaminostiryl)-1-methylpyridinium (DSM). Analysis of a competitive binding of DSM and ribonuclease with the erythrocyte ghosts has shown that irradiation causes an increase in the constant of protein association with membranes. It is suggested that a negative surface change increase with irradiation.
Asunto(s)
Membrana Eritrocítica/efectos de la radiación , Colorantes Fluorescentes , Compuestos de Piridinio/sangre , Ribonucleasas/sangre , Animales , Unión Competitiva , Membrana Eritrocítica/metabolismo , Membrana Eritrocítica/ultraestructura , RatasRESUMEN
Electrons with energy 5 MeV have been studied for their effect on the electrokinetic properties of erythrocytes. The electrophoretic mobility of the cells was shown to decrease under the effect of radiation. It is supposed that the effect observed is determined by the structural changes of the membrane proteins.
Asunto(s)
Electrones , Eritrocitos/efectos de la radiación , Animales , Electroforesis , RatasRESUMEN
Using fluorescent probe 4-(n-dimethyl-aminostyryl)-1-methyl-pyridine (DSM) the effect of electrons with the energy 5 MeV on the structure of erythrocyte membrane has been investigated. It was found that in the irradiated erythrocyte ghosts the number of DSM binding sites increases. Irradiation was suggested to increase the negative surface charge of the erythrocyte membrane.
Asunto(s)
Membrana Eritrocítica/efectos de la radiación , Animales , Sitios de Unión , Electrones , Membrana Eritrocítica/metabolismo , Colorantes Fluorescentes/metabolismo , Compuestos de Piridinio/metabolismo , RatasRESUMEN
Effect of electrons with the energy 5MeV on the erythrocyte ghosts structure has been investigated using fluorescent probes 1.8-ANS and DSP-12. The number of ANS binding sites was shown to decrease as affected by irradiation. It was found that radiation caused reduction of the erythrocyte membrane surface area.
Asunto(s)
Naftalenosulfonatos de Anilina/metabolismo , Electrones , Membrana Eritrocítica/efectos de la radiación , Colorantes Fluorescentes/metabolismo , Compuestos de Piridinio/metabolismo , Animales , Humanos , Radiación Ionizante , Ratas , Propiedades de SuperficieRESUMEN
Ultrasound effect on gramicidin incorporation into a bilayer lipid membrane has been investigated. The observed increase in the channel opening frequency points to the incorporation rate growth due to the thickness diminishing of near-membrane non-stirred layers. The dependence of ultrasound intensity on the layer thickness is presented.
Asunto(s)
Gramicidina/farmacocinética , Membrana Dobles de Lípidos , Ultrasonido , PermeabilidadRESUMEN
Ultrasonic effects (0.88 MHz, 0.8 Wt X cm-2) on Na+, K+-ATPase and Mg2+-ATPase changed the values KM, Vmax, delta H not equal to, delta S not equal to and the activation energy, which can point to some changes in the structure of lipoprotein enzyme complexes.