Port Baseline Biological Surveys and seaweed bioinvasions in port areas: What's the matter in the Adriatic Sea?

One of the objectives of the BALMAS project was to conduct Port Baseline Biological Surveys of native and non-indigenous benthic flora in 12 Adriatic ports. Samples of macroalgae growing on vertical artificial substrates were collected in spring and autumn 2014 and/or 2015. A total number of 248 taxa, 152 Rhodophyta, 62 Chlorophyta, and 34 Ochrophyta, were identified. Of these, 13 were non-indigenous seaweeds, mainly filamentous macroalgae, that were probably introduced through hull fouling. Some of these taxa had already been described in the study areas, others were recorded for the first time, a few were no longer detected at sites where they had previously been recorded (e.g. Sargassum muticum). Some other NISS reported for the Adriatic Sea, were not collected at any sampling site (i.e. Caulerpa cylindracea, Codium fragile). Possible reasons for the absence of these species are discussed.

Strategy of port baseline surveys (PBS) in the Adriatic Sea.

Port baseline surveys (PBS) provide species inventories in and around ports, with a focus on non-indigenous species that may have been introduced by vessels, primarily via ballast water. PBS are an essential tool to support effective management strategies for non-indigenous as well as native harmful aquatic organisms and pathogens (HAOP). This paper describes the methodology of PBS that were conducted in 12 Adriatic ports. The PBS employed existing protocols that were adapted to meet the characteristics of the Adriatic sites. Their results are reported in several papers included in this special issue, each of which is devoted to a specific community. An overview of existing surveys protocols - which provide valuable support to decision-making and to design effective monitoring of non-indigenous species - is also supplied.

A multidisciplinary weight of evidence approach for environmental risk assessment at the Costa Concordia wreck: Integrative indices from Mussel Watch.

A complex framework of chemical, biological and oceanographic activities was immediately activated after the Costa Concordia shipwreck, to assess possible contamination events and the environmental impact during both emergency and wreck removal operations. In the present paper, we describe the results obtained with caged mussels, Mytilus galloprovincialis, chosen as bioindicator organisms to detect variations of bioavailability and the early onset of molecular and cellular effects (biomarkers). Seven translocation experiments were carried out during the first year from the incident, with organisms deployed at 2 depths in 3 different sites. After 4-6 weeks, tissue concentrations were measured for the main classes of potentially released chemicals (trace metals, polycyclic aromatic hydrocarbons, volatile and aliphatic hydrocarbons, polychlorinated biphenyls, halogenated pesticides, organotin compounds, brominated flame retardants, anionic surfactants); a wide battery of biomarkers covered responses indicative of exposure, detoxification, oxidative stress, cell damage and genotoxic effects. Results excluded serious contamination events or a consistent increase of environmental pollution although some episodic spills with reversible effects were detected. Data were elaborated within a quantitative weight of evidence (WOE) model which provided synthetic hazard indices for each typology of data, before their overall integration in an environmental risk index, which generally ranged from slight to moderate. The proposed WOE model was confirmed a useful tool to summarize large datasets of complex data in integrative indices, and to simplify the interpretation for stakeholders and decision makers, thus supporting a more comprehensive process of "site-oriented" management decisions.

A methodological approach to understand functional relationships between ecological indices and human-induced pressures: the case of the Posidonia oceanica meadows.

The European Water Framework Directive (WFD) 2000/60/EC requests the achievement of the Good Status for all surface waters, including the coastal waters, by 2015. In order to check compliance with the needs of Directive, Italian national monitoring data on Posidonia oceanica meadows have been explored and the relationships among the Posidonia Rapid and Easy Index (PREI), and human-induced pressures have been analyzed along the Italian coasts. The aim of this work is to establish functional relationships between a response variable (i.e. the PREI) and a set of potential pressure (i.e. land use, potential organic and nutrient loading, pesticides) and status (i.e. transparency, trophic level and stability of the water column) indicators in a quantitative way. The ecological responses of coastal marine environment have been evaluated using appropriate statistical tools, such as the multiple linear regression analyses and "linear programming" techniques. Results show that more than 70% of the variability of the P. oceanica meadows status, expressed as PREI value, is significantly explained only by a few pressure/status indicators (namely: potential organic load, specific nitrogen load, natural areas extent, water column transparency), among all those initially considered in the model. The application of the proposed model could allow decision makers to better address remedial actions and to achieve the environmental targets proposed by the EU Directives.