RESUMEN
Learned experiences are not necessarily consolidated into long-term memory (LTM) unless they are periodic and meaningful. LTM depends on de novo protein synthesis mediated by cyclic AMP response element-binding protein (CREB) activity. In Drosophila, two creb genes (crebA, crebB) and multiple CREB isoforms have reported influences on aversive olfactory LTM in response to multiple cycles of spaced conditioning. How CREB isoforms regulate LTM effector genes in various neural elements of the memory circuit is unclear, especially in the mushroom body (MB), a prominent associative center in the fly brain that has been shown to participate in LTM formation. Here, we report that i) spaced training induces crebB expression in MB α-lobe neurons and ii) elevating specific CREBB isoform levels in the early α/ß subpopulation of MB neurons enhances LTM formation. By contrast, learning from weak training iii) induces 5-HT1A serotonin receptor synthesis, iv) activates 5-HT1A in early α/ß neurons, and v) inhibits LTM formation. vi) LTM is enhanced when this inhibitory effect is relieved by down-regulating 5-HT1A or overexpressing CREBB. Our findings show that spaced training-induced CREBB antagonizes learning-induced 5-HT1A in early α/ß MB neurons to modulate LTM consolidation.
Asunto(s)
Proteínas de Drosophila , Cuerpos Pedunculados , Animales , Cuerpos Pedunculados/fisiología , Drosophila/metabolismo , Proteínas de Drosophila/metabolismo , Memoria a Largo Plazo/fisiología , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/genética , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Drosophila melanogaster/metabolismoRESUMEN
Long-term memory (LTM) formation requires consolidation processes to overcome interfering signals that erode memory formation. Olfactory memory in Drosophila involves convergent projection neuron (PN; odor) and dopaminergic neuron (DAN; reinforcement) input to the mushroom body (MB). How post-training DAN activity in the posterior lateral protocerebrum (PPL1) continues to regulate memory consolidation remains unknown. Here we address this question using targeted transgenes in behavior and electrophysiology experiments to show that (1) persistent post-training activity of PPL1-α2α'2 and PPL1-α3 DANs interferes with aversive LTM formation; (2) neuropeptide F (NPF) signaling blocks this interference in PPL1-α2α'2 and PPL1-α3 DANs after spaced training to enable LTM formation; and (3) training-induced NPF release and neurotransmission from two upstream dorsal-anterior-lateral (DAL2) neurons are required to form LTM. Thus, NPF signals from DAL2 neurons to specific PPL1 DANs disinhibit the memory circuit, ensuring that periodic events are remembered as consolidated LTM.
RESUMEN
Episodic events are frequently consolidated into labile memory but are not necessarily transferred to persistent long-term memory (LTM). Regulatory mechanisms leading to LTM formation are poorly understood, however, especially at the resolution of identified neurons. Here, we demonstrate enhanced LTM following aversive olfactory conditioning in Drosophila when the transcription factor cyclic AMP response element binding protein A (CREBA) is induced in just two dorsal-anterior-lateral (DAL) neurons. Our experiments show that this process is regulated by protein-gene interactions in DAL neurons: (1) crebA transcription is induced by training and repressed by crebB overexpression, (2) CREBA bidirectionally modulates LTM formation, (3) crebA overexpression enhances training-induced gene transcription, and (4) increasing membrane excitability enhances LTM formation and gene expression. These findings suggest that activity-dependent gene expression in DAL neurons during LTM formation is regulated by CREB proteins.
Asunto(s)
Proteína de Unión al Elemento de Respuesta al AMP Cíclico/metabolismo , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Proteínas de Drosophila/metabolismo , Memoria a Largo Plazo/fisiología , Transactivadores/metabolismo , Animales , Condicionamiento Clásico/fisiología , Condicionamiento Psicológico , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/genética , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/fisiología , Proteína de Unión al Elemento de Respuesta al AMP Cíclico/genética , Proteína de Unión al Elemento de Respuesta al AMP Cíclico/fisiología , Proteínas de Drosophila/fisiología , Drosophila melanogaster , Femenino , Expresión Génica/genética , Regulación de la Expresión Génica/genética , Masculino , Neuronas/metabolismo , Neuronas/fisiología , Percepción Olfatoria/fisiología , Olfato/fisiología , Transactivadores/fisiologíaRESUMEN
Parkinson's disease (PD) is a progressive motor neurodegenerative disorder, characterized by a selective loss of dopaminergic neurons in the substantia nigra. The complexity of disease etiology includes both genetic and environmental factors. No effective drug that can modify disease progression and protect dopamine neurons from degeneration is presently available. Human α-Synuclein A30P (A30P) is a mutant gene identified in early onset PD and showed to result selective dopamine neuron loss in transgenic A30P flies and mice. Paraquat (PQ) is an herbicide and an oxidative stress generator, linked to sporadic PD. We hypothesized that vital PD modifier genes are conserved across species and would show unique transcriptional changes to oxidative stress in animals expressing a PD-associated gene, such as A30P. We also hypothesized that manipulation of PD modifier genes would provide neuroprotection across species. To identify disease modifier genes, we performed two independently-duplicated experiments of microarray, capturing genome-wide transcriptional changes in A30P flies, chronically fed with PQ-contaminated food. We hypothesized that the best time point of identifying a disease modifier gene is at time when flies showed maximal combined toxicity of A30P transgene and PQ treatment during an early stage of disease and that effective disease modifiers gene are those showing transcriptional changes to oxidative stress in A30P expressing and not in wild type animals. Fly Neprilysin3 (Nep3) is one identified gene that is highly conserved. Its mouse and human homolog is endothelin-converting enzyme-1 (Ece1). To investigate the neuroprotective effect of Ece1, we used NS1 cells and mouse midbrain neurons expressing A30P, treated with or without PQ. We found that ECE1 expression protected against A30P toxicity on cell viability, on neurite outgrowth and ameliorated A30P accumulation in vitro. Expression of ECE1 in vivo suppressed dopamine neuron loss and alleviated the corresponding motor deficits in mice with A30P-expression. Our study leverages a new approach to identify disease modifier genes using a stress-enhanced PD animal model.
Asunto(s)
Neuronas Dopaminérgicas/metabolismo , Enzimas Convertidoras de Endotelina/biosíntesis , Estrés Oxidativo/fisiología , alfa-Sinucleína/biosíntesis , alfa-Sinucleína/toxicidad , Secuencia de Aminoácidos , Animales , Animales Modificados Genéticamente , Animales Recién Nacidos , Células Cultivadas , Neuronas Dopaminérgicas/efectos de los fármacos , Método Doble Ciego , Drosophila , Enzimas Convertidoras de Endotelina/genética , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , alfa-Sinucleína/genéticaRESUMEN
The testing of cognitive enhancers could benefit from the development of novel behavioural tasks that display better translational relevance for daily memory and permit the examination of potential targets in a within-subjects manner with less variability. We here outline an optimized spatial 'everyday memory' task. We calibrate it systematically by interrogating certain well-established determinants of memory and consider its potential for revealing novel features of encoding-related gene activation. Rats were trained in an event arena in which food was hidden in sandwells in a different location everyday. They found the food during an initial memory-encoding trial and were then required to remember the location in six alternative choice or probe trials at various time-points later. Training continued daily over a period of 4 months, realizing a stable high level of performance and characterized by delay-dependent forgetting over 24 h. Spaced but not massed access to multiple rewards enhanced the persistence of memory, as did post-encoding administration of the PDE4 inhibitor Rolipram. Quantitative PCR and then genome-wide analysis of gene expression led to a new observation - stronger gene-activation in hippocampus and retrosplenial cortex following spaced than massed training. In a subsidiary study, a separate group of animals replicated aspects of this training profile, going on to show enhanced memory when training was subject to post-encoding environmental novelty. Distinctive features of this protocol include its potential validity as a model of memory encoding used routinely by human subjects everyday, and the possibility of multiple within-subject comparisons to speed up assays of novel compounds.
Asunto(s)
Recuerdo Mental/fisiología , Nootrópicos/farmacología , Recompensa , Investigación Biomédica Traslacional/métodos , Animales , Corteza Cerebral/fisiología , Perfilación de la Expresión Génica/métodos , Habituación Psicofisiológica/efectos de los fármacos , Habituación Psicofisiológica/fisiología , Hipocampo/efectos de los fármacos , Hipocampo/fisiología , Masculino , Memoria/efectos de los fármacos , Memoria/fisiología , Recuerdo Mental/efectos de los fármacos , RatasRESUMEN
Parkinson's disease (PD) is the most common motor neurodegenerative disorder. Olfactory dysfunction is a prevalent feature of PD. It often precedes motor symptoms by several years and is used in assisting PD diagnosis. However, the cellular and molecular bases of olfactory dysfunction in PD are not known. The fruit fly Drosophila melanogaster, expressing human alpha-synuclein protein or its mutant, A30P, captures several hallmarks of PD and has been successfully used to model PD in numerous studies. First, we report olfactory deficits in fly expressing A30P (A30P), showing deficits in two out of three olfactory modalities, tested--olfactory acuity and odor discrimination. The remaining third modality is odor identification/naming. Second, oxidative stress is an important environmental risk factor of PD. We show that oxidative stress exacerbated the two affected olfactory modalities in younger A30P flies. Third, different olfactory receptor neurons are activated differentially by different odors in flies. In a separate experiment, we show that the odor discrimination deficit in A30P flies is general and not restricted to a specific class of chemical structure. Lastly, by restricting A30P expression to dopamine, serotonin or olfactory receptor neurons, we show that A30P expression in dopamine neurons is necessary for development of both acuity and discrimination deficits, while serotonin and olfactory receptor neurons appeared not involved. Our data demonstrate olfactory deficits in a synuclein fly PD model for exploring olfactory pathology and physiology, and for monitoring PD progression and treatment.
Asunto(s)
Drosophila melanogaster , Percepción Olfatoria , Enfermedad de Parkinson/genética , Enfermedad de Parkinson/fisiopatología , alfa-Sinucleína/genética , Envejecimiento/fisiología , Animales , Discriminación en Psicología , Modelos Animales de Enfermedad , Neuronas Dopaminérgicas/patología , Humanos , Actividad Motora , Estrés Oxidativo , Enfermedad de Parkinson/metabolismo , Enfermedad de Parkinson/patologíaRESUMEN
Aging is associated with declines in memory and cognitive function. Here, we evaluate the effects of HT-0712 on memory formation and on cAMP response element-binding protein (CREB)-regulated genes in aged mice. HT-0712 enhanced long-term memory formation in normal young mice at brain concentrations similar to those found to increase CRE-mediated gene expression in hippocampal neurons. Aged mice showed significantly poorer contextual and trace conditioning compared with young-adult mice. In aged mice, a single injection of HT-0712 significantly boosted contextual and trace long-term memory. Additional effects of HT-0712 were seen in a spatial memory task. Our parallel biochemical experiments revealed that inductions of the CREB-regulated genes, cFos, Zif268, and Bdnf, after fear conditioning were diminished in aged mice. HT-0712 facilitated expression of these CREB-regulated genes in aged hippocampus, indicating that the drug engages a CREB-regulated mechanism in vivo. These findings corroborate and extend our previous results on the mechanism of action of HT-0712 and its efficacy to enhance memory formation. Our data also indicate that HT-0712 may be effective to treat age-associated memory impairment in humans.
Asunto(s)
Envejecimiento/efectos de los fármacos , Condicionamiento Psicológico/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Hipocampo/fisiología , Memoria a Largo Plazo/efectos de los fármacos , Inhibidores de Fosfodiesterasa 4/farmacología , Factores de Edad , Animales , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Proteína de Unión a CREB/metabolismo , Células Cultivadas , Proteína 1 de la Respuesta de Crecimiento Precoz/metabolismo , Embrión de Mamíferos , Conducta Exploratoria/efectos de los fármacos , Miedo/psicología , Masculino , Aprendizaje por Laberinto/efectos de los fármacos , Ratones , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Piperidonas/farmacología , Unión Proteica/efectos de los fármacos , Proteínas Proto-Oncogénicas c-fos/metabolismo , Rolipram/farmacologíaRESUMEN
Although the benefits of spaced retrieval for long-term retention are well established, the majority of this work has involved spacing over relatively short intervals (on the order of seconds or minutes). In the present experiments, we evaluated the effectiveness of spaced retrieval across relatively short intervals (within a single session), as compared to longer intervals (between sessions spaced a day apart), for long-term retention (i.e., one day or one week). Across a series of seven experiments, participants (N = 536) learned paired associates to a criterion of 70 % accuracy and then received one test-feedback trial for each item. The test-feedback trial occurred within 10 min of reaching criterion (short lag) or one day later (long lag). Then, a final test occurred one day (Exps. 1-3) or one week (Exps. 4 and 5) after the test-feedback trial. Across the different materials and methods in Experiments 1-3, we found little benefit for the long-lag relative to the short-lag schedule in final recall performance-that is, no lag effect-but large effects on the retention of information from the test-feedback to the final test phase. The results from the experiments with the one-week retention interval (Exps. 4 and 5) indicated a benefit of the long-lag schedule on final recall performance (a lag effect), as well as on retention. This research shows that even when the benefits of lag are eliminated at a (relatively long) one-day retention interval, the lag effect reemerges after a one-week retention interval. The results are interpreted within an extension of the bifurcation model to the spacing effect.
Asunto(s)
Aprendizaje por Asociación/fisiología , Recuerdo Mental/fisiología , Retención en Psicología/fisiología , Adulto , Humanos , Factores de TiempoRESUMEN
Memory is initially labile and gradually consolidated over time through new protein synthesis into a long-lasting stable form. Studies of odor-shock associative learning in Drosophila have established the mushroom body (MB) as a key brain structure involved in olfactory long-term memory (LTM) formation. Exactly how early neural activity encoded in thousands of MB neurons is consolidated into protein-synthesis-dependent LTM remains unclear. Here, several independent lines of evidence indicate that changes in two MB vertical lobe V3 (MB-V3) extrinsic neurons are required and contribute to an extended neural network involved in olfactory LTM: (i) inhibiting protein synthesis in MB-V3 neurons impairs LTM; (ii) MB-V3 neurons show enhanced neural activity after spaced but not massed training; (iii) MB-V3 dendrites, synapsing with hundreds of MB α/ß neurons, exhibit dramatic structural plasticity after removal of olfactory inputs; (iv) neurotransmission from MB-V3 neurons is necessary for LTM retrieval; and (v) RNAi-mediated down-regulation of oo18 RNA-binding protein (involved in local regulation of protein translation) in MB-V3 neurons impairs LTM. Our results suggest a model of long-term memory formation that includes a systems-level consolidation process, wherein an early, labile olfactory memory represented by neural activity in a sparse subset of MB neurons is converted into a stable LTM through protein synthesis in dendrites of MB-V3 neurons synapsed onto MB α lobes.
Asunto(s)
Proteínas de Drosophila/fisiología , Drosophila melanogaster/fisiología , Regulación de la Expresión Génica , Memoria a Largo Plazo/fisiología , Cuerpos Pedunculados/fisiología , Proteínas de Unión al ARN/fisiología , Animales , Cruzamientos Genéticos , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/metabolismo , Modelos Neurológicos , Cuerpos Pedunculados/metabolismo , Neuronas/metabolismo , Proteínas de Unión al ARN/metabolismo , Transmisión Sináptica , Factores de Transcripción/metabolismo , Factores de Escisión y Poliadenilación de ARNm/metabolismoRESUMEN
Long-term memory (LTM) depends on the synthesis of new proteins. Using a temperature-sensitive ribosome-inactivating toxin to acutely inhibit protein synthesis, we screened individual neurons making new proteins after olfactory associative conditioning in Drosophila. Surprisingly, LTM was impaired after inhibiting protein synthesis in two dorsal-anterior-lateral (DAL) neurons but not in the mushroom body (MB), which is considered the adult learning and memory center. Using a photoconvertible fluorescent protein KAEDE to report de novo protein synthesis, we have directly visualized cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional activation of calcium/calmodulin-dependent protein kinase II and period genes in the DAL neurons after spaced but not massed training. Memory retention was impaired by blocking neural output in DAL during retrieval but not during acquisition or consolidation. These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB).
Asunto(s)
Proteínas de Drosophila/biosíntesis , Drosophila/fisiología , Memoria a Largo Plazo/fisiología , Cuerpos Pedunculados/fisiología , Neuronas/fisiología , Animales , Animales Modificados Genéticamente , Axones/ultraestructura , Encéfalo/citología , Encéfalo/fisiología , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina/biosíntesis , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina/genética , Condicionamiento Psicológico , Criptocromos/biosíntesis , Criptocromos/genética , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/genética , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Drosophila/citología , Drosophila/genética , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Genes de Insecto , Proteínas Luminiscentes/biosíntesis , Proteínas Luminiscentes/genética , Neuronas/ultraestructura , Odorantes , Proteínas Circadianas Period/biosíntesis , Proteínas Circadianas Period/genética , Ricina/farmacología , Células Receptoras Sensoriales/fisiología , Serina Endopeptidasas/biosíntesis , Serina Endopeptidasas/genética , Transactivadores/genética , Transactivadores/metabolismo , Activación TranscripcionalRESUMEN
We developed a novel assay to examine social interactions in Drosophila and, as a first attempt, apply it here at examining the behavior of Drosophila Fragile X Mental Retardation gene (dfmr1) mutants. Fragile X syndrome is the most common cause of single gene intellectual disability (ID) and is frequently associated with autism. Our results suggest that dfmr1 mutants are less active than wild-type flies and interact with each other less often. In addition, mutants for one allele of dfmr1, dfmr1(B55), are more likely to come in close contact with a wild-type fly than another dfmr1(B55) mutant. Our results raise the possibility of defective social expression with preserved receptive abilities. We further suggest that the assay may be applied in a general strategy of examining endophenoypes of complex human neurological disorders in Drosophila, and specifically in order to understand the genetic basis of social interaction defects linked with ID.
Asunto(s)
Proteínas de Drosophila/genética , Drosophila/genética , Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/genética , Conducta Social , Animales , Endofenotipos , Femenino , Masculino , Modelos Animales , Mutación , Espacio PersonalRESUMEN
The last decade has witnessed the identification of single-gene defects associated with an impressive number of mental retardation syndromes. Fragile X syndrome, the most common cause of mental retardation for instance, results from disruption of the FMR1 gene. Similarly, Periventricular Nodular Heterotopia, which includes cerebral malformation, epilepsy and cognitive disabilities, derives from disruption of the Filamin A gene. While it remains unclear whether defects in common molecular pathways may underlie the cognitive dysfunction of these various syndromes, defects in cytoskeletal structure nonetheless appear to be common to several mental retardation syndromes. FMR1 is known to interact with Rac, profilin, PAK and Ras, which are associated with dendritic spine defects. In Drosophila, disruptions of the dFmr1 gene impair long-term memory (LTM), and the Filamin A homolog (cheerio) was identified in a behavioral screen for LTM mutants. Thus, we investigated the possible interaction between cheerio and dFmr1 during LTM formation in Drosophila. We show that LTM specifically is defective in dFmr1/cheerio double heterozygotes, while it is normal in single heterozygotes for either dFmr1 or cheerio. In dFmr1 mutants, Filamin (Cheerio) levels are lower than normal after spaced training. These observations support the notion that decreased actin cross-linking may underlie the persistence of long and thin dendritic spines in Fragile X patients and animal models. More generally, our results represent the first demonstration of a genetic interaction between mental retardation genes in an in vivo model system of memory formation.
RESUMEN
Mental retardation--known more commonly nowadays as intellectual disability--is a severe neurological condition affecting up to 3% of the general population. As a result of the analysis of familial cases and recent advances in clinical genetic testing, great strides have been made in our understanding of the genetic etiologies of mental retardation. Nonetheless, no treatment is currently clinically available to patients suffering from intellectual disability. Several animal models have been used in the study of memory and cognition. Established paradigms in Drosophila have recently captured cognitive defects in fly mutants for orthologs of genes involved in human intellectual disability. We review here three protocols designed to understand the molecular genetic basis of learning and memory in Drosophila and the genes identified so far with relation to mental retardation. In addition, we explore the mental retardation genes for which evidence of neuronal dysfunction other than memory has been established in Drosophila. Finally, we summarize the findings in Drosophila for mental retardation genes for which no neuronal information is yet available. All in all, this review illustrates the impressive overlap between genes identified in human mental retardation and genes involved in physiological learning and memory.
Asunto(s)
Drosophila/genética , Drosophila/fisiología , Discapacidad Intelectual/genética , Animales , Conducta Animal , Femenino , Genes de Insecto , Humanos , Aprendizaje , Masculino , Memoria , Modelos Animales , Modelos Psicológicos , Mutación , Fenotipo , Especificidad de la EspecieRESUMEN
Genetic screens for Drosophila mutants defective in pavlovian olfactory memory have provided unique insight into the molecular basis of memory storage. Occasionally, these singular genetic lesions have been assembled into meaningful molecular pathways and neural circuitries. For the most part, however, these genes and their expression patterns in the CNS remain fragmented, demanding new clues from continued mutant screens. From a behavioral screen for long-term memory (LTM) mutants, we have identified ben (CG32594), which encodes a novel protein. Mutations of ben specifically disrupt LTM, leaving earlier memory phases intact. The role of ben appears physiological rather than developmental, because acutely induced expression of a ben(+) transgene in adults rescues the mutant's LTM defect. More interestingly, induced expression of ben(+) specifically in mushroom bodies (MBs), but not in the ellipsoid body of the central complex, is sufficient to rescue the mutant LTM defect. This suggests a role for ben in the MB during olfactory memory formation. We also provide evidence that BEN interacts genetically in both synaptic transmission and LTM formation with SCAMP, a synaptic protein known to be involved in vesicle recycling.
Asunto(s)
Proteínas Portadoras/fisiología , Proteínas de Drosophila/fisiología , Trastornos de la Memoria/genética , Memoria/fisiología , Transmisión Sináptica/genética , Enzimas Ubiquitina-Conjugadoras/fisiología , Análisis de Varianza , Animales , Animales Modificados Genéticamente , Conducta Animal , Proteínas Portadoras/genética , Condicionamiento Clásico/fisiología , Drosophila/genética , Proteínas de Drosophila/genética , Regulación de la Expresión Génica/genética , Larva , Actividad Motora/genética , Cuerpos Pedunculados/metabolismo , Mutación/genética , Unión Neuromuscular/genética , Vías Olfatorias/fisiología , ARN Mensajero/metabolismo , Enzimas Ubiquitina-Conjugadoras/genéticaRESUMEN
The ruslan (rus) mutant was previously identified in a behavioral screen for mutants defective in long-lasting memory, which consists of two consolidated memory types, anesthesia-resistant memory, and protein synthesis-dependent long-term memory (LTM). We demonstrate here that rus is a new allele of klingon (klg), which encodes a homophilic cell adhesion molecule. Klg is acutely required for LTM but not anesthesia-resistant memory formation, and Klg expression increases upon LTM induction. LTM formation also requires activity of the Notch cell-surface receptor. Although defects in Notch have been implicated in memory loss because of Alzheimer's disease, downstream signaling linking Notch to memory have not been determined. Strikingly, we found that Notch activity increases upon LTM induction and regulates Klg expression. Furthermore, Notch-induced enhancement of LTM is disrupted by a klg mutation. We propose that Klg is a downstream effector of Notch signaling that links Notch activity to memory.
Asunto(s)
Moléculas de Adhesión Celular/fisiología , Proteínas de Drosophila/fisiología , Proteínas del Ojo/fisiología , Memoria , Receptores Notch/fisiología , Anestesia/efectos adversos , Animales , Moléculas de Adhesión Celular/genética , Drosophila/fisiología , Proteínas de Drosophila/genética , Proteínas del Ojo/genética , Regulación de la Expresión Génica/fisiología , Memoria/efectos de los fármacos , Mutación , Transducción de SeñalRESUMEN
We used Drosophila olfactory memory as a model to study the molecular basis of cognitive defects in Fragile X syndrome in vivo. We observed that fragile X protein was acutely required and interacted with argonaute1 and staufen in the formation of long-term memory. Occlusion of long-term memory formation in Fragile X mutants could be rescued by protein synthesis inhibitors, suggesting that excess baseline protein synthesis could negatively affect cognition.
Asunto(s)
Modelos Animales de Enfermedad , Síndrome del Cromosoma X Frágil/complicaciones , Síndrome del Cromosoma X Frágil/metabolismo , Trastornos de la Memoria/etiología , Biosíntesis de Proteínas/fisiología , Análisis de Varianza , Animales , Animales Modificados Genéticamente , Conducta Animal , Condicionamiento Clásico/fisiología , Drosophila , Proteínas de Drosophila/genética , Electrochoque , Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil/genética , Mutación , Vías Olfatorias/fisiología , Biosíntesis de Proteínas/efectos de los fármacos , Interferencia de ARN , Percepción Espacial/fisiología , Factores de TiempoRESUMEN
Angelman syndrome is a neurological disorder whose symptoms include severe mental retardation, loss of motor coordination, and sleep disturbances. The disease is caused by a loss of function of UBE3A, which encodes a HECT-domain ubiquitin ligase. Here, we generate a Drosophila model for the disease. The results of several experiments show that the functions of human UBE3A and its fly counterpart, dube3a, are similar. First, expression of Dube3a is enriched in the Drosophila nervous system, including mushroom bodies, the seat of learning and memory. Second, we have generated dube3a null mutants, and they appear normal externally, but display abnormal locomotive behavior and circadian rhythms, and defective long-term memory. Third, flies that overexpress Dube3a in the nervous system also display locomotion defects, dependent on the ubiquitin ligase activity. Finally, missense mutations in UBE3A alleles of Angelman syndrome patients alter amino acid residues conserved in the fly protein, and when introduced into dube3a, behave as loss-of-function mutations. The simplest model for Angelman syndrome is that in the absence of UBE3A, particular substrates fail to be ubiquitinated and proteasomally degraded, accumulate in the brain, and interfere with brain function. We have generated flies useful for genetic screens to identify Dube3a substrates. These flies overexpress Dube3a in the eye or wing and display morphological abnormalities, dependent on the critical catalytic cysteine. We conclude that dube3a mutants are a valid model for Angelman syndrome, with great potential for identifying the elusive UBE3A substrates relevant to the disease.
Asunto(s)
Síndrome de Angelman/genética , Proteínas de Drosophila/genética , Ubiquitina-Proteína Ligasas/genética , Animales , Ritmo Circadiano/genética , Modelos Animales de Enfermedad , Drosophila , Proteínas de Drosophila/fisiología , Ojo/química , Expresión Génica , Humanos , Locomoción/genética , Morfogénesis/genética , Mutación , Sistema Nervioso/química , Fenotipo , Ubiquitina-Proteína Ligasas/fisiología , Alas de Animales/químicaRESUMEN
Drosophila Pumilio (Pum) protein is a translational regulator involved in embryonic patterning and germline development. Recent findings demonstrate that Pum also plays an important role in the nervous system, both at the neuromuscular junction (NMJ) and in long-term memory formation. In neurons, Pum appears to play a role in homeostatic control of excitability via down regulation of para, a voltage gated sodium channel, and may more generally modulate local protein synthesis in neurons via translational repression of eIF-4E. Aside from these, the biologically relevant targets of Pum in the nervous system remain largely unknown. We hypothesized that Pum might play a role in regulating the local translation underlying synapse-specific modifications during memory formation. To identify relevant translational targets, we used an informatics approach to predict Pum targets among mRNAs whose products have synaptic localization. We then used both in vitro binding and two in vivo assays to functionally confirm the fidelity of this informatics screening method. We find that Pum strongly and specifically binds to RNA sequences in the 3'UTR of four of the predicted target genes, demonstrating the validity of our method. We then demonstrate that one of these predicted target sequences, in the 3'UTR of discs large (dlg1), the Drosophila PSD95 ortholog, can functionally substitute for a canonical NRE (Nanos response element) in vivo in a heterologous functional assay. Finally, we show that the endogenous dlg1 mRNA can be regulated by Pumilio in a neuronal context, the adult mushroom bodies (MB), which is an anatomical site of memory storage.
Asunto(s)
Proteínas de Drosophila/química , Proteínas de Drosophila/metabolismo , Neuronas/química , Neuronas/metabolismo , Análisis de Secuencia de Proteína/métodos , Sinapsis/química , Sinapsis/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Datos de Secuencia Molecular , Unión Proteica , Proteínas de Unión al ARNRESUMEN
BACKGROUND: It has become increasingly clear that molecular and neural mechanisms underlying learning and memory and drug addiction are largely shared. To confirm and extend these findings, we analyzed ethanol-responsive behaviors of a collection of Drosophila long-term memory mutants. METHODS: For each mutant, sensitivity to the acute uncoordinating effects of ethanol was quantified using the inebriometer. Additionally, 2 distinct forms of ethanol tolerance were measured: rapid tolerance, which develops in response to a single brief exposure to a high concentration of ethanol vapor; and chronic tolerance, which develops following a sustained low-level exposure. RESULTS: Several mutants were identified with altered sensitivity, rapid or chronic tolerance, while a number of mutants exhibited multiple defects. CONCLUSIONS: The corresponding genes in these mutants represent areas of potential overlap between learning and memory and behavioral responses to alcohol. These genes also define components shared between different ethanol behavioral responses.
Asunto(s)
Conducta Animal/efectos de los fármacos , Drosophila melanogaster/efectos de los fármacos , Etanol/farmacología , Aprendizaje/efectos de los fármacos , Memoria/efectos de los fármacos , Animales , Drosophila melanogaster/genética , Tolerancia a Medicamentos/genética , Etanol/farmacocinéticaRESUMEN
We describe the identification of linotte, a new autosomal gene in Drosophila involved with learning and memory. The linotte(1) mutant was derived from a PlacW transposan mutagenesis and was screened for three-hour memory deficits after classical conditioning of an olfactory avoidance response. Sensory and motor systems (olfactory acuity and shock reactivity) required for the classical conditioning experiments were normal in mutant linotte(1) files--indicating that the mutation disrupts learning/memory specifically. A chromosomal deficiency of the 37D region, where the linotte(1)P insert was localized in situ, failed to complement linotte(1)'s memory defect, and files from two lines homozygous for independent PlacW excisions show normal memory--indicating that the P insertion is responsible for the mutant phenotype. Additional behavior-genetic data suggest that linotte gene is non-vital.