Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon.

A yellow-colored, Gram-stain-positive, rod shaped, non-motile bacterium, designated as strain JC619T, was isolated from the sediment of Chilika lagoon, India. Strain JC619T shows highest 16S rRNA gene sequence similarity (99.08%) with Isoptericola chiayiensis KCTC 19740T followed by Isoptericola halotolerans KCTC 19046T (98.6%) and other members of the genus Isoptericola (< 98%). NaCl is required for growth of strain JC619T and tolerates up to 18% (w/v) and pH up to 10. Strain JC619T grows optimally at temperature 30 °C, NaCl concentration of 3% (w/v), and at pH 7.5. The genome size of strain JC619T is 3.2 Mb with G+C content of 73.0 mol%. ANI scores of strain JC619T are 81.9% and 80.1% and dDDH values are 24.4% and 22.7% with I. chiayiensis KCTC 19740T and I. halotolerans KCTC 19046T, respectively. Respiratory quinones are MK-9(H4) and MK-9(H2). Predominant fatty acids (> 10%) are anteiso-C15:0, iso-C16:0, and iso-C15:0. Major polar lipids of strain JC619T are phosphatidylglycerol, phosphatidylinositolmannoside, diphosphatidylglycerol, and phosphatidylinositol. Strain JC619T is catalase positive but cytochrome oxidase negative and reduces nitrate. The genomic distinction of strain JC619T with its nearest related species of the genus Isoptericola is well supported with chemotaxonomic characteristics and differential physiological properties. Therefore, strain JC619T represents a new species under the genus Isoptericola for which Isoptericola sediminis sp. nov. is proposed. Type strain is JC619T (=KCTC 49244T =NBRC 114063T).

Roseimaritima sediminicola sp. nov., a new member of Planctomycetaceae isolated from Chilika lagoon.

Strain JC651T was isolated from a sediment sample collected from Chilika lagoon, which is one of the world's most important brackish water lakes with estuarine characteristics. Colonies of this strain are light pink and cells are Gram-stain negative, spherical to pear shaped and form rosettes. Strain JC651T grows well up to pH 9.0 and tolerates up to 5 % NaCl (w/v). The respiratory quinone is MK6. The detected major fatty acids are C18 : 1 ω9c and C16 : 0. Its polar lipids are diphosphatidylglycerol, an unidentified phospholipid, phosphatidylglycerol and phosphatidylcholine. Strain JC651T shows highest 16S rRNA gene sequence similarity (97.8%) to the type species of the genus Roseimaritima, Roseimaritima ulvae UC8T. The genome size of strain JC651T is 6.2 Mb with a G+C content of 62.4 mol%. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also reconstructed with the sequences of 92 core genes. Based on the phylogenetic analyses, low digital DNA-DNA hybridization values (19.5%), low (74.9%) genome average nucleotide identity results, chemotaxonomic characteristics and differential physiological properties, strain JC651T is recognized as a new species of the genus Roseimaritima for which we propose the name Roseimaritima sediminicola sp. nov. The type strain is JC651T (=KCTC 72178T=NBRC 113926T).