1.
Chem Commun (Camb)
; 58(95): 13282-13285, 2022 Nov 29.
Artículo
en Inglés
| MEDLINE
| ID: mdl-36373598
RESUMEN
A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N'-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.