RESUMEN
The influence of exogenic human recombinant tumor necrosis factor (TNF-alpha) on antibody production in mice immunized with the preparation of F.tularensis outer membranes (OM) was studied. TNF-alpha was injected into mice in doses of 0.001-10 I.U. before and simultaneously with the injection of the preparation of F.tularensis OM. The levels of tularemia antibodies, determined by ELISA techniques, and the number of antibody-producing cells (APC) were studied. The study revealed that recombinant TNF-alpha in the range of doses used in this investigation stimulated the formation of humoral immunity. The injection of TNF-alpha in a dose of 0.001 I.U. was found to produce the most pronounced effect on the level of tularemia antibodies and the number of antibody-producing cells. The use TNF-alpha as immunomodulator made it possible to decrease the dose of the preparation of F.tularensis OM introduced as immunogenic agent without essential changes in the number of splenic APC and in the level of tularemia antibodies.
Asunto(s)
Anticuerpos Antibacterianos/efectos de los fármacos , Proteínas de la Membrana Bacteriana Externa/inmunología , Francisella tularensis/inmunología , Inmunización/métodos , Factor de Necrosis Tumoral alfa/farmacología , Animales , Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Antibacterianos/sangre , Células Productoras de Anticuerpos/efectos de los fármacos , Células Productoras de Anticuerpos/inmunología , Relación Dosis-Respuesta a Droga , Relación Dosis-Respuesta Inmunológica , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos CBA , Proteínas Recombinantes/farmacología , Estimulación Química , Factores de TiempoAsunto(s)
Peste/etiología , Factor de Necrosis Tumoral alfa/farmacología , Animales , Citotoxicidad Celular Dependiente de Anticuerpos/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Humanos , Sueros Inmunes/farmacología , Ratones , Ratones Endogámicos CBA , Peste/inmunología , Ratas , Proteínas Recombinantes/farmacología , Factores de Tiempo , Factor de Necrosis Tumoral alfa/inmunologíaRESUMEN
The activity of NK and K cells in mice immunized against plague has been studied. This activity has been shown to depend on the dose of the vaccine and the time elapsed after immunization. The booster immunization of mice leads to an increase in the specific sensitization of lymphocytes, the antibody level and to an increase in the activity of killer cells. Changes in the cytotoxicity of NK cells on days 21-28 after primary and booster immunization are considered to occur not due to the action of Yersinia pestis themselves, but as the result of the immunological transformation induced by these microorganisms in the animals.
Asunto(s)
Células Asesinas Naturales/inmunología , Vacuna contra la Peste/inmunología , Peste/prevención & control , Animales , Anticuerpos Antibacterianos/sangre , Relación Dosis-Respuesta Inmunológica , Tolerancia Inmunológica , Inmunización Secundaria , Inyecciones Subcutáneas , Activación de Linfocitos , Ratones , Ratones Endogámicos CBA , Vacuna contra la Peste/administración & dosificación , Factores de Tiempo , Yersinia pestis/inmunologíaRESUMEN
Changes in the activity of NK and K cells in persons immunized against plague have been studied. A decrease in the activity of natural killer cells has been shown to occur. Booster immunization leads to a greater increase in the specific sensitization of lymphocytes, as well as in the antibody level. The observed increase of the activity of killer cells is regarded not as the result of the direct action of live Yersinia pestis cells, but as the result of the immunological changes in the body which they produce.
