RESUMEN
The aim of this study was the development of a methodology for the integral study of the antagonistic activity of normal human microbiota against Staphylococcus aureus to enable direct selection (without prior isolation of pure cultures) of potentially highly efficient probiotic preparations. The selection of bacterial representatives of normal human nasal microbiota capable of antagonizing S. aureus was carried out using two complimentary methods: replica-plating and deferred antagonism procedures. The material of the anterior nares from healthy human subjects was plated onto the surface of different nutrient media agar plates followed by incubation under appropriate conditions. The grown bacterial colonies were replica-plated to Petri dishes with nutrient agar overlayed with a thin layer of a soft agar which contained the culture of an indicator S. aureus strain. This agar also supported the growth of potential probiotic strains. The potential probiotic strains were selected by their ability to suppress the growth of S. aureus around their colonies. Most active strains-inhibitors may be used to develop probiotic preparations with targeted activity against S. aureus.
Asunto(s)
Probióticos , Infecciones Estafilocócicas , Humanos , Staphylococcus aureus , Agar , Medios de Cultivo , Probióticos/farmacologíaRESUMEN
INTRODUCTION: The phenomenon of therapeutic and prophylactic effect of natural mineral waters (MW) used to restore tissue tolerance to glucose and normalize basal insulin secretion in metabolic syndrome is well known. At the same time, the use of selenium-enriched MW is promising due to its multitarget action including participation in the regulation of the protein-synthesizing function of cells. AIM: To study the effect of internal course intake of selenium-modified mineral waters of different composition on bone mineral density after long-term diet-induced metabolic disorders in the experiment. MATERIAL AND METHODS: The study was carried out on outbred male white rats that were distributed into 3 control groups (CG) and 4 main groups (MG). In CG1 14 animals received laboratory food and drinking water; in CG2 14 animals were modeled for diet-induced metabolic disorders, and they were withdrawn from the experiment the day after the cancellation of the hypercaloric diet (180 days); after the cancellation of the hypercaloric diet 180 days later 14 animals with diet-induced metabolic disorders in CG3 had a standard diet (laboratory food and drinking water) for 30 days. In the experimental groups (EG), the animals similarly to CG3 were modeled diet-induced metabolic disorders, after the cancellation of the diet they had a standard diet and watering with MW. In MG1 18 animals were given MW1 (Essentuki-4); in MG2 19 animals had a course of MW1Se (Essentuki-4 enriched with selenium); in MG3 14 animals had a course of MW2 (Slavyanovskaya); in MG4 18 animals had a course of MB2Se (Slavyanovskaya enriched with selenium). The weight (body weight) of the animals was monitored monthly. Biochemical studies, densitometry and computed microtomography were used to determine the effectiveness of MW. RESULTS: The characteristics of spongy bone tissue of femoral metaphysis of the rats of the control groups revealed disorders of varying degrees, but, despite this, the bone tissue structure of the examined vertebral bodies in animals without a hyper caloric diet (CG1) was preserved. The analysis of the study results demonstrated a significant effectiveness of selenium-enriched MW in regulating metabolic processes (p<0.05-p<0.01), bone mineral density (p<0.05), reduced bone resorption (p<0.05), that is, the characteristics of spongy bone tissue of femoral metaphysis and vertebral bodies of animals showed a clear tendency to restore bone structure. CONCLUSION: The results of this study make it possible to predict an increase in the effectiveness of medical rehabilitation of patients with somatic diseases when selenium-enriched MW is included in the programmes.
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Agua Potable , Enfermedades Metabólicas , Aguas Minerales , Selenio , Humanos , Masculino , Animales , Ratas , Selenio/farmacología , Densidad Ósea , DietaRESUMEN
OBJECTIVE: To evaluate the effect of the preventive course of drinking mineral water enriched with selenium on the processes of resistance to the damaging action of reversible occlusion of the anterior mesenteric artery based on the comparison of intestinal morphological changes in the experiment. MATERIAL AND METHODS: There has been modeled ischemic reperfusion injury of the intestinal wall according to H. Ikeda and co-authors using reversible occlusion of the anterior mesenteric artery with 33 outbred male rats. The rats were divided into four groups by block randomization: the 1st group - intact animals (n=7) - without an exposure; the control group - sham operated animals (n=6); the group of comparison (n=7) - with a model-operation; the experimental group (n=11) - animals with a model operation that had courses of intragastric watering of bottled sulfate-chloride-hydrocarbonate-sodium low-mineralized (2.2 g/l) drinking mineral water «Psyzh¼ enriched with selenium. Biopsies of the small intestine were taken for histological examination. RESULTS: Histological examination of the small intestine of experimental animals determines various degrees of severity of damage: on average, the animals of the experimental group on the scale of C.J. Chiu (1970) had the lowest degree of severity of pathological changes, the animals of the group of comparison - 1.4 times higher (p=0.02). That is, the effect of a preventive course of mineral water «Psyzh¼ enriched with selenium was manifested in the formation of resistance to the damaging effect of reversible occlusion of the anterior mesenteric artery; in the presence of ischemic reperfusion damage to the intestinal wall, comparable in severity to changes with the animals without prevention, the most significant positive effect was realized in the containment of reactive changes. CONCLUSION: The effect of the preventive course of drinking mineral water «Psyzh¼ enriched with selenium manifested itself in the formation of resistance to the damaging effect of reversible occlusion of the anterior mesenteric artery, which is the basis for introducing this technique into clinical practice in order to prevent the development of reperfusion injuries of the intestine.
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Aguas Minerales , Selenio , Ratas , Masculino , Animales , Ratas Wistar , Selenio/farmacología , Intestino Delgado/irrigación sanguínea , Intestino Delgado/patología , Arterias MesentéricasRESUMEN
The purpose of the work was to analyze the pro-inflammatory reaction caused by different duration of ischemic-reperfusion of intestinal damage in occlusion of anterior mesenteric artery in the experiment. Ischemic reperfusion damage of the intestinal wall was simulated using reversible occlusion of the anterior mesenteric artery with 30 rats. Two types of operations were carried out: operation 1 - the duration of ischemia 20 minutes, reperfusion - 40 minutes, operation 2 - 30 and 30 minutes, respectively. Four groups were formed by simple sampling: the first - false-operated animals (n=5), the second - model-operation 1 (n=8), the third - model-operation 2 (n=6), the 4th - control group (n=7). Lactate level was determined by BM-Lactat test strips and the number of blood leukocytes was calculated before and after the surgery. During the postoperative period, interleukin-1ß (IL-1ß), tumor necrosis factor α (TNF-α), monocytic chemotoxic factor-1 (MCF-1) in serum were determined. In groups 2 and 3 it was established: a decrease in leukocytes in peripheral blood by 15-20% and an increase in lactate level by 20-120% during the postoperative period with the establishment of direct statistically significant connections between the number of leukocytes in the blood and lactate content (r=+0.83, p=0.01); between the number of leukocytes in the blood and IL-1ß blood level (r=+0.96; p=0,00002); reduction of IL-1ß level in blood with the animals of group 3 as compared with false-operated and MCF-1 level with the animals of group 2 as compared with false-operated, which may be related to the influence of corrective action of anti-inflammatory response. The increase of TNF-a level with the animals of the second group by 1.5-2 times and in the third group by 3 times compared to false-operated animals, which is the most significant manifestation of pro-inflammatory response to ischemia/reperfusion, depends on the degree of the damage due to the duration of ischemic period. The development of systemic pro-inflammatory reactions in the simulation of reversible occlusion of anterior mesenteric artery is the basis for the rehabilitation of patients after various surgical interventions in the intestine in order to prevent abdominal postoperative complications.
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Daño por Reperfusión , Animales , Humanos , Isquemia , Ácido Láctico , Arterias Mesentéricas/patología , Arterias Mesentéricas/cirugía , Ratas , Daño por Reperfusión/prevención & control , Factor de Necrosis Tumoral alfaRESUMEN
The purpose of the study was to determine the effect of native and selenium-modified mineral waters on metabolic syndrome models. Combined metabolic syndrome (combMS) was simulated in 70 white rats of male gender under standard vivarium conditions, using high-calorie food and single administration of streptozotocin. During the random sampling study the following groups of animals were formed: in the control group 1 (n=6) the rats had standard forage during the experiment; in the control group 2 (n=10) there were rats with combMS model without treatment; in the main groups, starting at the 61st day of combMS, the rats received: the 1st group (n=11) - Essentuki mineral water â4; the 2nd (n=16) - the same water with selenium; in the 3rd (n=11) - Slavyanovskaya mineral water; in the 4th (n=16) - Slavyanovskaya with selenium. Registration of the effects was determined by carbohydrate, protein and lipid metabolism. Metabolic syndrome was monitored by recording animal weight gain and the level of glycemia by the express method at the beginning and the end of the treatment course. As a result, in main groups glucose increased 1.5-2 times, urea and lactate - 1.5 times, triglycerides - 30-40% compared, to the data in the control group 1, against the background of reduced content of contrinsular hormones. All the animals with combMS (control 2 and all the main groups) increased α-amylase activity. The most significant corrective parameters of the metabolic syndrome were established: in the main group 1 - a decrease in hyperglycemia, in the 3rd main group - urea, and in the main groups 2 and 4 the lipid spectrum recovered and the level of lactate decreased. Modification of different mineral waters by selenium provides leveling of differences between them in therapeutic effectiveness.
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Síndrome Metabólico , Aguas Minerales , Selenio , Animales , Ácido Láctico , Masculino , Síndrome Metabólico/terapia , Aguas Minerales/uso terapéutico , Ratas , Selenio/farmacología , UreaRESUMEN
The objective of the present study was to develop a new technology for the balneotherapeutic treatment of the patients with urolithiasis by means of combined application of mineral water, mineral baths (1 therapeutic complex), sinusoidal modulated current (SMC-phoresis of nicotinamide), and preparations inhibiting urine concrement formation. The overall efficacy of this combined balneo-, physio-, radio-, and pharmacotherapy of the patients presenting with secondary calculous pyelonephritis amounted to 94% compared with 73.4% using balneotherapy alone (p1-2 < 0.05). Summation of positive effects of balneo-, physio-, radio-, and pharmacotherapies promoted normalization of the functional state of the kidneys and the upper urinary tracts which in its turn contributed to the improvement of the patient's quality of life.
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Balneología/métodos , Cálculos Renales/rehabilitación , Pielonefritis/rehabilitación , Recuperación de la Función , Adulto , Enfermedad Crónica , Femenino , Humanos , Riñón/fisiopatología , Cálculos Renales/fisiopatología , Masculino , Persona de Mediana Edad , Pielonefritis/fisiopatologíaRESUMEN
Etiological factors and pathogenetic mechanisms were studied in 538 patients with nephrolithiasis. By composition of uroliths and salts, all the patients were divided into groups by types of urolithiasis (oxalate, urate, phosphate, cystic and mixed). After elimination of the uroliths using different methods, metaphylaxis of nephrolithiasis was conducted in all the patients. It varied with type of lithogenesis, etiology and pathogenesis. Antirecurrence therapy included diet with restriction of lithogenic substances, endogenic reduction of lithogenic metabolites and measures to decrease and block crystallization and lithogenesis. The treatment has diminished the incidence of recurrences from 32.3 to 9.6% (total), from 14.4 to 5.3% (in oxalate type), 47.2 to 16.9% (in phosphate type), 39.7 to 6.7% (in urate type), 40.5 to 17.7% (in mixed type).