RESUMEN
BACKGROUND: The life cycle of most baleen whales involves annual migrations from low-latitude breeding grounds to high latitude feeding grounds. In most species, these migrations are traditionally considered to be carried out according to information acquired through vertical social learning during the first months of life and made individually. However, some recent studies have suggested a more complex scenario, particularly for the species of the Balaenoptera genus. METHODS: Here, we studied the variation of δ15N and δ13C values along the growth axis of the baleen plate from 24 fin whales feeding off western Iceland to delve into their pattern of movements and to identify potential associations between individuals. The segment of baleen plate analyzed informed about at least two complete migratory cycles. We performed cluster analyses through two different methodologies and, whenever possible, we genotyped 20 microsatellite loci to determine potential existence of kinship. RESULTS: Results of the of δ15N and δ13C values agree with a dispersion strategy in the winter breeding grounds. However, and despite the overall large variability, several pairs or groups of individuals with no kinship showed highly similar isotopic patterns for two consecutive years for both δ15N and δ13C values. CONCLUSIONS: Our results suggest that, notably, some whales without kinship share the same migratory regime and destinations. We hypothesize that this could reflect either: (i) the sharing of particularly beneficial migratory regimes, and/or (ii) long-term association between individuals.
RESUMEN
RATIONALE: Stable isotope analysis of growth layers in sperm whale teeth dentine can provide valuable insight into individual long-distance displacements and diet. Although treating teeth half-sections with formic acid and rubbing their surface with a graphite pencil improves growth layer visibility and reduces sampling error, previous studies mostly used untreated half-sections as the effect that this treatment may have on stable isotope ratios in dentine is unknown. The present study investigates the treatment effect on stable C and N isotope ratios in sperm whale teeth dentine. METHODS: In the teeth of 30 sperm whales, we analysed and compared samples of powdered dentine obtained from (a) untreated half-sections, (b) half-sections etched with formic acid and rubbed with a graphite pencil and (c) half-sections etched with formic acid from which the graphite pencil rubbing had been cleansed off. δ 13 $$ {\delta}^{13} $$ C and δ 15 $$ {\delta}^{15} $$ N values were compared between the three sample groups. RESULTS: We found significant differences in values of both elements between untreated and etched samples, with a mean increase of 0.2% in δ13 C and δ15 N values in the etched samples. No significant differences were found between etched samples with graphite rubbing versus those without it. Significant linear regression models were calculated to predict untreated δ13 C and δ15 N values from the values obtained in the etched half-sections with limited precision. CONCLUSIONS: We show for the first time that formic acid etching has a clear effect on δ 13 $$ {\delta}^{13} $$ C and δ 15 $$ {\delta}^{15} $$ N values in sperm whale teeth dentine. The developed models permit the estimation of untreated values from etched half-sections, thus enabling the use of the latter in stable isotope analysis. However, as treatment procedures may vary between studies, it is advisable that similar predictive models are developed case-by-case to ensure comparability of results.
Asunto(s)
Carbono , Grafito , Animales , Isótopos de Nitrógeno/análisis , Nitrógeno , Cachalote , Isótopos de Carbono/análisis , Dentina/químicaRESUMEN
Two major oceanographic changes have recently propagated through several trophic levels in coastal areas of Southeast Greenland (SEG). Firstly, the amount of drift-ice exported from the Fram Strait and transported with the East Greenland Current (EGC) has decreased significantly over the past two decades, and a main tipping element (summer sea ice) has virtually disappeared since 2003 leading to a regime shift in oceanographic and ecological conditions in the region. The following 20-year period with low or no coastal sea ice is unique in the 200-year history of ice observations in the region, and the regime shift is also obvious in the volume of ice export through the Fram Strait after 2013. In the same period, the temperature of the EGC south of 73.5 N has increased significantly (>2°C) since 1980. Secondly, the warm Irminger Current, which advects warm, saline Atlantic Water into the region, has become warmer since 1990. The lack of pack ice in summer together with a warming ocean generated cascading effects on the ecosystem in SEG that are manifested in a changed fish fauna with an influx of boreal species in the south and the subarctic capelin further north. At higher trophic levels there has been an increase in the abundance of several boreal cetaceans (humpback, fin, killer, and pilot whales and dolphins) that are either new to this area or occur in historically large numbers. It is estimated that the new cetacean species in SEG are responsible for an annual predation level of 700,000 tons of fish. In addition, predation on krill species is estimated at >1,500,000 tons mainly consumed by fin whales. Simultaneously, there has been a reduction in the abundance and catches of narwhals and walruses in SEG and it is suggested that these species have been impacted by the habitat changes.
Asunto(s)
Ecosistema , Ballenas , Animales , Groenlandia , Estaciones del Año , Temperatura , Peces , Cubierta de HieloRESUMEN
Marine mammals are one of the groups of animals most affected by marine pollution including that by organic compounds which, besides bearing recognised harmful effects to adults, they may also affect foetuses through placental transfer. In this study we analysed samples of fin whale (Balaenoptera physalus) females and their foetuses collected in the western Iceland summer feeding grounds in 2018. Three different families of organic pollutants: organophosphate esters (OPEs); halogenated flame retardants (HFRs); and short chain chlorinated paraffins (SCCPs), were analysed and their placental transfer investigated. HFRs were detected in 87.5% of females and 100% of foetus samples with concentrations that ranged between nd-15.4 and 6.37-101 ng/g lipid weight (lw), respectively. OPEs were detected in all samples, both from females (85.8-567 ng/g lw) and foetuses (nq-1130 ng/g lw). SCCPs were detected in 87.5% of female samples and 100% of foetal samples with concentrations that ranged between nd-30.9 and nq-574 ng/g lw, respectively. For OPE compounds, a significant negative correlation was observed between the logarithm maternal transfer ratio and their log Kow, indicating that a high lipophilicity reduced placental transfer rate. Interestingly, the decabromodiphenyl ethane (DBDPE) was the compound with the highest log Kow but also the one that was transferred the most from mothers to foetuses, calling for in-depth research on this pollutant. These results constitute the first evidence of mother-calf transfer of plasticizers and flame retardants in fin whales. Further investigations are needed to determine their potential effects on this species and other groups of animals.
Asunto(s)
Contaminantes Ambientales , Ballena de Aleta , Retardadores de Llama , Animales , Femenino , Embarazo , Océano Atlántico , Monitoreo del Ambiente , Contaminantes Ambientales/análisis , Ésteres/análisis , Retardadores de Llama/análisis , Éteres Difenilos Halogenados/análisis , Lípidos , Organofosfatos/análisis , Placenta/química , Plastificantes/análisisRESUMEN
The fin whale (Balaenoptera physalus) is a migratory filter-feeding species that is susceptible to ingest plastics while lunge feeding across the oceans. Plastic additives, such as phthalates, are compounds that are added to plastics to give them specific characteristics, such as flexibility. These so-called plasticizers are currently raising major concern because of their potential adverse effects on marine fauna. However, little is known about phthalate concentrations in tissues of baleen whales as well as their potential relation with biological variables (i.e., sex, body length and age) and their trends with time. In this study, we assessed the concentration of 13 phthalates in the muscle of 31 fin whales sampled in the feeding grounds off western Iceland between 1986 and 2015. We detected 5 of the 13 phthalates investigated, with di-n-butylphthalate (DBP), diethylphthalate (DEP) and bis(2-ethylhexyl) phthalate (DEHP) being the most abundant. None of the biological variables examined showed a statistically significant relationship with phthalate concentrations. Also, phthalate concentrations did not significantly vary over the 29-year period studied, a surprising result given the global scenario of increasing plastic pollution in the seas. The lack of time trends in phthalate concentration may be due in part to the fact that phthalates also originate from other sources. Although no adverse effects of phthalates on fin whales have been detected to date, further monitoring of these pollutants is required to identify potential toxic effects in the future.
Asunto(s)
Ballena de Aleta , Animales , Islandia , Ácidos Ftálicos , PlásticosRESUMEN
There has been a considerable number of reports on Hg concentrations in Arctic mammals since the last Arctic Monitoring and Assessment Programme (AMAP) effort to review biological effects of the exposure to mercury (Hg) in Arctic biota in 2010 and 2018. Here, we provide an update on the state of the knowledge of health risk associated with Hg concentrations in Arctic marine and terrestrial mammal species. Using available population-specific data post-2000, our ultimate goal is to provide an updated evidence-based estimate of the risk for adverse health effects from Hg exposure in Arctic mammal species at the individual and population level. Tissue residues of Hg in 13 species across the Arctic were classified into five risk categories (from No risk to Severe risk) based on critical tissue concentrations derived from experimental studies on harp seals and mink. Exposure to Hg lead to low or no risk for health effects in most populations of marine and terrestrial mammals, however, subpopulations of polar bears, pilot whales, narwhals, beluga and hooded seals are highly exposed in geographic hotspots raising concern for Hg-induced toxicological effects. About 6% of a total of 3500 individuals, across different marine mammal species, age groups and regions, are at high or severe risk of health effects from Hg exposure. The corresponding figure for the 12 terrestrial species, regions and age groups was as low as 0.3% of a total of 731 individuals analyzed for their Hg loads. Temporal analyses indicated that the proportion of polar bears at low or moderate risk has increased in East/West Greenland and Western Hudson Bay, respectively. However, there remain numerous knowledge gaps to improve risk assessments of Hg exposure in Arctic mammalian species, including the establishment of improved concentration thresholds and upscaling to the assessment of population-level effects.
Asunto(s)
Caniformia , Mercurio , Phocidae , Ursidae , Animales , Regiones Árticas , Monitoreo del Ambiente , Mamíferos , Mercurio/toxicidad , Medición de RiesgoRESUMEN
Global warming is affecting the population dynamics and trophic interactions across a wide range of ecosystems and habitats. Translating these real-time effects into their long-term consequences remains a challenge. The rapid and extreme warming period that occurred after the Last Glacial Maximum (LGM) during the Pleistocene-Holocene transition (7-12 thousand years ago) provides an opportunity to gain insights into the long-term responses of natural populations to periods with global warming. The effects of this post-LGM warming period have been assessed in many terrestrial taxa, whereas insights into the impacts of rapid global warming on marine taxa remain limited, especially for megafauna. In order to understand how large-scale climate fluctuations during the post-LGM affected baleen whales and their prey, we conducted an extensive, large-scale analysis of the long-term effects of the post-LGM warming on abundance and inter-ocean connectivity in eight baleen whale and seven prey (fish and invertebrates) species across the Southern and the North Atlantic Ocean; two ocean basins that differ in key oceanographic features. The analysis was based upon 7032 mitochondrial DNA sequences as well as genome-wide DNA sequence variation in 100 individuals. The estimated temporal changes in genetic diversity during the last 30,000 years indicated that most baleen whale populations underwent post-LGM expansions in both ocean basins. The increase in baleen whale abundance during the Holocene was associated with simultaneous changes in their prey and climate. Highly correlated, synchronized and exponential increases in abundance in both baleen whales and their prey in the Southern Ocean were indicative of a dramatic increase in ocean productivity. In contrast, the demographic fluctuations observed in baleen whales and their prey in the North Atlantic Ocean were subtle, varying across taxa and time. Perhaps most important was the observation that the ocean-wide expansions and decreases in abundance that were initiated by the post-LGM global warming, continued for millennia after global temperatures stabilized, reflecting persistent, long-lasting impacts of global warming on marine fauna.
Asunto(s)
Ecosistema , Calentamiento Global , Animales , Océano Atlántico , Dinámica Poblacional , Ballenas/fisiologíaRESUMEN
In this study we aim to assess the daily ingestion rates of synthetic particles by the fin whales (Balaenoptera physalus) that feed off the western coast of Iceland. To do so, we collected and analysed samples from the stomach content of 25 fin whales, consisting solely of northern krill (Meganyctiphanes norvegica). The particles found consisted of fibres and fragments, mainly blue, black and red, with an average size of 1.2 ± 1.3 mm. To confirm the synthetic nature of these particles, we used Micro-Fourier Transform Infrared Spectroscopy and comparison with a polymer library. The mean concentration of synthetic particles in the krill samples found in the stomachs of whales was 0.057 particles per gram, a value much lower than that previously reported for particle uptake by krill. From this concentration in krill, we estimated that the daily intake of synthetic particles for the North Atlantic fin whale would be ranging from 38,646 ± 43,392 to 77,292 ± 86,784 particles per day. Although at this level it is not possible to assess the impact of synthetic particles and their associated chemicals on the North Atlantic fin whale population, concentrations of these contaminants are likely to increase in the future, potentially causing adverse effects on whales and other marine mammals.
Asunto(s)
Ballena de Aleta , Animales , Ingestión de Alimentos , Islandia , Estaciones del Año , BallenasRESUMEN
Biodiversity in the oceans has dramatically declined since the beginning of the industrial era, with accelerated loss of marine biodiversity impairing the ocean's capacity to maintain vital ecosystem services. A few organisms epitomize the damaging and long-lasting effects of anthropogenic exploitation: Some whale species, for instance, were brought to the brink of extinction, with their population sizes reduced to such low levels that may have caused a significant disruption to their reproductive dynamics and facilitated hybridization events. The incidence of hybridization is nevertheless believed to be rare, and very little information exists on its directionality. Here, using genetic markers, we show that all but one whale hybrid sample collected in Icelandic waters originated from the successful mating of male fin whale and female blue whale, thus suggesting unidirectional hybridization. We also demonstrate for the first time the existence of a second-generation adult (male) hybrid resulting from a backcross between a female hybrid and a pure male fin whale. The incidence of hybridization events between fin and blue whales is likely underestimated and the observed unidirectional hybridization (for F1 and F2 hybrids) is likely to induce a reproductive loss in blue whale, which may represent an additional challenge to its recovery in the Atlantic Ocean compared to other rorquals.
RESUMEN
Interindividual variation in prey specialization is an essential yet overlooked aspect of wildlife feeding ecology, especially as it relates to intrapopulation variation in exposure to toxic contaminants. Here, we assessed blubber concentrations of an extensive suite of persistent organic pollutants in Icelandic killer whales (Orcinus orca). Polychlorinated biphenyl (PCB) concentrations in blubber were >300-fold higher in the most contaminated individual relative to the least contaminated, ranging from 1.3 to 428.6 mg·kg-1 lw. Mean PCB concentrations were 6-to-9-fold greater in individuals with a mixed diet including marine mammals than in fish specialist individuals, whereas males showed PCB concentrations 4-fold higher than females. Given PCBs have been identified as potentially impacting killer whale population growth, and levels in mixed feeders specifically exceeded known thresholds, the ecology of individuals must be recognized to accurately forecast how contaminants may threaten the long-term persistence of the world's ultimate marine predator.
Asunto(s)
Bifenilos Policlorados , Contaminantes Químicos del Agua , Orca , Animales , Monitoreo del Ambiente , Femenino , Islandia , Bifenilos Policlorados/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
Historical variation in food resources is expected to be a major driver of cetacean evolution, especially for the smallest species like porpoises. Despite major conservation issues among porpoise species (e.g., vaquita and finless), their evolutionary history remains understudied. Here, we reconstructed their evolutionary history across the speciation continuum. Phylogenetic analyses of 63 mitochondrial genomes suggest that porpoises radiated during the deep environmental changes of the Pliocene. However, all intra-specific subdivisions were shaped during the Quaternary glaciations. We observed analogous evolutionary patterns in both hemispheres associated with convergent evolution to coastal versus oceanic environments. This suggests that similar mechanisms are driving species diversification in northern (harbor and Dall's) and southern species (spectacled and Burmeister's). In contrast to previous studies, spectacled and Burmeister's porpoises shared a more recent common ancestor than with the vaquita that diverged from southern species during the Pliocene. The low genetic diversity observed in the vaquita carried signatures of a very low population size since the last 5,000 years. Cryptic lineages within Dall's, spectacled and Pacific harbor porpoises suggest a richer evolutionary history than previously suspected. These results provide a new perspective on the mechanisms driving diversification in porpoises and an evolutionary framework for their conservation.
Asunto(s)
Evolución Biológica , Genoma Mitocondrial/genética , Genómica/métodos , Mitocondrias/genética , Marsopas/fisiología , Animales , Biodiversidad , Ecosistema , Evolución Molecular , Especiación Genética , Filogenia , Especificidad de la EspecieRESUMEN
Pollution of the marine environment by litter composed of plastics is a growing concern. Chemical additives such as organophosphate flame retardants (OPFRs), which are added to plastics to improve their qualities, are in focus because they allegedly cause adverse effects on marine fauna. Here we analyse OPFR levels in the muscle of fin whales because, as a mysticete, this cetacean obtains its food by filter-feeding and is thus highly vulnerable to marine litter. Moreover, the fin whale performs long-range migrations from low-latitude areas in winter to high-latitude areas in summer, a trait that makes it a potentially good large-scale biomonitor of pollution. We also analyse OPFR levels in its main prey, the krill Meganyctiphanes norvegica, to assess transfer through diet. The samples analysed consisted of muscle tissue from 20 fin whales and whole-body homogenates of 10 krill samples, all collected off West Iceland. From the 19 OPFRs analysed, we detected 7 in the fin whale and 5 in the krill samples. Tri-n-butyl phosphate (TNBP), Isopropylated triphenyl phosphate (IPPP) and Triphenylphosphine oxide (TPPO) were the most abundant compounds found in both species. Mean ∑OPFR concentration, expressed on a lipid weight basis, was 985 (SD = 2239) ng g-1 in fin whale muscle, and 949 (SD = 1090) ng g-1 in krill homogenates. These results constitute the first evidence of the presence of OPFRs in the tissues of fin whales. Furthermore, they seem to support the non-significance of bioaccumulation of OPFRs through lifespan and of biomagnification trough the food web.
Asunto(s)
Ballena de Aleta , Retardadores de Llama , Animales , Islandia , Organofosfatos , PlásticosRESUMEN
Highly migratory marine species pose a challenge for the identification of management units due to the absence of clear oceanographic barriers. The population structure of North Atlantic fin whales has been investigated since the start of whaling operations but is still the subject of an ongoing scientific debate. Here we measured stable isotopes of carbon, nitrogen and oxygen in skin samples collected from 151 individuals from western Iceland, Galicia (NW Spain), the Azores archipelago and the Strait of Gibraltar (SoG). We found spatiotemporal differences in stable isotope ratios suggesting that fin whales sampled in these four areas may share a common feeding ground within the Northeast Atlantic at different times during the year. Our results also suggest that SoG whales use this common feeding ground in summer but exploit Mediterranean resources during the winter months, further supporting the existence of a limited but current exchange of individuals between these two basins.
Asunto(s)
Ballena de Aleta , Cadena Alimentaria , Animales , Azores , Gibraltar , Islandia , EspañaRESUMEN
Killer whales have a cosmopolitan distribution and as a species are generalists, feeding on a variety of prey. However, local populations tend to specialise on specific prey types. In Icelandic waters, killer whales are generally associated with herring and, thus, have been presumed to be herring specialists. However, recent studies suggest a more complex foraging ecology, possibly including a mosaic of strategies. With increased observational effort in recent years due to research and whale-watching activities, there have been several reports of interactions with different prey, including confirmed predation events. In this study we aimed to summarise the range of potential prey of killer whales observed in Icelandic waters. We report on 12 previously unpublished accounts and review 15 accounts published in the scientific literature or local newspapers, making a total of 27 events where killer whales were observed interacting with actual or potential prey. Thirteen different species, including birds (n = 1), cephalopods (n = 1), fish (n = 5) and marine mammals (n = 6), are reported, although herring is by far the species that killer whales are most often observed interacting with. This study provides the first summary of actual and suspected killer whale prey in Icelandic waters, and contributes towards our understanding of this population's prey preferences. However, describing the diet of individuals/groups was not possible and this study points to a need for continued monitoring to understand the intricacies of killer whale foraging behaviour in this area.
Asunto(s)
Conducta Predatoria/fisiología , Orca/fisiología , Animales , IslandiaRESUMEN
RATIONALE: Baleen plates are anatomical structures composed of inert tissue that hang from the upper jaw in mysticetes. Baleen plates may differ in size and in coloration between different segments of the filtering row or between sides of the mouth. Concern has been raised that variation in baleen plate characteristics may reflect dissimilar structural composition and growth rates liable to affect stable isotope ratios and their oscillation patterns. METHODS: We measured stable carbon (δ13 C values) and nitrogen (δ15 N values) isotope ratios at intervals of 1 cm along the longitudinal axis of six baleen plates collected from different positions along the mouth of a fin whale. All samples were analysed using a continuous flow isotope ratio mass spectrometer. Generalized additive models were fitted to the data from each baleen plate and the results of the models were compared visually. RESULTS: A total of 206 samples were analysed. Visually, all baleen plates presented nearly identical oscillations, independent of the position or the coloration of the baleen plate. However, the variation in δ13 C and δ15 N values occurring between the different baleen plates was higher in the segments of oscillations exhibiting steeper slopes. CONCLUSIONS: Differences in size between plates in an individual are due to differential erosion rates according to their position in the mouth. Therefore, the position of sampling along the baleen plate row should not be a reason for concern when conducting stable isotope studies.
Asunto(s)
Estructuras Animales/química , Isótopos de Carbono/análisis , Ballena de Aleta/fisiología , Boca/química , Isótopos de Nitrógeno/análisis , Animales , Conducta Alimentaria , Masculino , Espectrometría de MasasRESUMEN
The population structure of the highly mobile marine mammal, the harbor porpoise (Phocoena phocoena), in the Atlantic shelf waters follows a pattern of significant isolation-by-distance. The population structure of harbor porpoises from the Baltic Sea, which is connected with the North Sea through a series of basins separated by shallow underwater ridges, however, is more complex. Here, we investigated the population differentiation of harbor porpoises in European Seas with a special focus on the Baltic Sea and adjacent waters, using a population genomics approach. We used 2872 single nucleotide polymorphisms (SNPs), derived from double digest restriction-site associated DNA sequencing (ddRAD-seq), as well as 13 microsatellite loci and mitochondrial haplotypes for the same set of individuals. Spatial principal components analysis (sPCA), and Bayesian clustering on a subset of SNPs suggest three main groupings at the level of all studied regions: the Black Sea, the North Atlantic, and the Baltic Sea. Furthermore, we observed a distinct separation of the North Sea harbor porpoises from the Baltic Sea populations, and identified splits between porpoise populations within the Baltic Sea. We observed a notable distinction between the Belt Sea and the Inner Baltic Sea sub-regions. Improved delineation of harbor porpoise population assignments for the Baltic based on genomic evidence is important for conservation management of this endangered cetacean in threatened habitats, particularly in the Baltic Sea proper. In addition, we show that SNPs outperform microsatellite markers and demonstrate the utility of RAD-tags from a relatively small, opportunistically sampled cetacean sample set for population diversity and divergence analysis.
Asunto(s)
Genoma , Phocoena/genética , Análisis de Varianza , Animales , Teorema de Bayes , ADN/química , ADN/aislamiento & purificación , ADN/metabolismo , Variación Genética , Repeticiones de Microsatélite/genética , Mar del Norte , Polimorfismo de Nucleótido Simple , Análisis de Componente Principal , Análisis de Secuencia de ADNRESUMEN
Despite no obvious barriers to gene flow in the marine realm, environmental variation and ecological specializations can lead to genetic differentiation in highly mobile predators. Here, we investigated the genetic structure of the harbour porpoise over the entire species distribution range in western Palearctic waters. Combined analyses of 10 microsatellite loci and a 5085 base-pair portion of the mitochondrial genome revealed the existence of three ecotypes, equally divergent at the mitochondrial genome, distributed in the Black Sea (BS), the European continental shelf waters, and a previously overlooked ecotype in the upwelling zones of Iberia and Mauritania. Historical demographic inferences using approximate Bayesian computation (ABC) suggest that these ecotypes diverged during the last glacial maximum (c. 23-19 kilo-years ago, kyrbp). ABC supports the hypothesis that the BS and upwelling ecotypes share a more recent common ancestor (c. 14 kyrbp) than either does with the European continental shelf ecotype (c. 28 kyrbp), suggesting they probably descended from the extinct populations that once inhabited the Mediterranean during the glacial and post-glacial period. We showed that the two Atlantic ecotypes established a narrow admixture zone in the Bay of Biscay during the last millennium, with highly asymmetric gene flow. This study highlights the impacts that climate change may have on the distribution and speciation process in pelagic predators and shows that allopatric divergence can occur in these highly mobile species and be a source of genetic diversity.
Asunto(s)
Cambio Climático , Ecotipo , Variación Genética , Genética de Población , Phocoena/genética , Animales , Océano Atlántico , Teorema de Bayes , ADN Mitocondrial/genética , Flujo Génico , Genotipo , Mar Mediterráneo , Repeticiones de Microsatélite , Modelos Genéticos , Phocoena/clasificación , Densidad de Población , Dinámica Poblacional , Análisis de Secuencia de ADNRESUMEN
The appeal of genetic inference methods to assess population genetic structure and guide management efforts is grounded in the correlation between the genetic similarity and gene flow among populations. Effects of such gene flow are typically genomewide; however, some loci may appear as outliers, displaying above or below average genetic divergence relative to the genomewide level. Above average population, genetic divergence may be due to divergent selection as a result of local adaptation. Consequently, substantial efforts have been directed toward such outlying loci in order to identify traits subject to local adaptation. Here, we report the results of an investigation into the molecular basis of the substantial degree of genetic divergence previously reported at allozyme loci among North Atlantic fin whale (Balaenoptera physalus) populations. We sequenced the exons encoding for the two most divergent allozyme loci (MDH-1 and MPI) and failed to detect any nonsynonymous substitutions. Following extensive error checking and analysis of additional bioinformatic and morphological data, we hypothesize that the observed allozyme polymorphisms may reflect phenotypic plasticity at the cellular level, perhaps as a response to nutritional stress. While such plasticity is intriguing in itself, and of fundamental evolutionary interest, our key finding is that the observed allozyme variation does not appear to be a result of genetic drift, migration, or selection on the MDH-1 and MPI exons themselves, stressing the importance of interpreting allozyme data with caution. As for North Atlantic fin whale population structure, our findings support the low levels of differentiation found in previous analyses of DNA nucleotide loci.
RESUMEN
Seasonal trends in energy storage of the minke whale (Balaenoptera acutorostrata), a capital breeder, were investigated in Iceland, a North Atlantic feeding ground. The aim was to better understand the energy acquisition strategies of minke whales and the energetic costs that different reproductive classes face during the breeding season. We modelled total blubber volume, using blubber thickness and morphometric measurements of individual whales. Blubber volume was influenced by body length, and was higher for pregnant females than mature whales. Blubber volume increased linearly through the feeding season at the same rate for mature (mean ± s.e.m.=0.0028 ± 0.00103 m(3) day(-1); N=61 male, 5 female) and pregnant whales (0.0024 ± 0.00100 m(3) day(-1); N=49), suggesting that minke whales aim to maximise energy storage while on the feeding grounds. The total amount of blubber accumulated over the feeding season (0.51 ± 0.119 m(3) for mature and 0.43 ± 0.112 m(3) for pregnant whales), together with energy stored as muscle and intra-abdominal fats, constitutes the total amount of energy available for reproduction (fetus development and lactation) on the breeding grounds, as well as migration, daily field metabolic rates, growth and body maintenance. No seasonal variation was observed for immature whales (N=4 male, 12 female), suggesting that they are investing most of their excess energy into growth rather than reproduction, in order to reach the length of sexual maturity faster and start reproducing earlier. Our novel modelling approach provides insight into large whale bioenergetics and life history strategies, as well as the relationship between single-site measurement of blubber thickness and total blubber volume.
Asunto(s)
Tejido Adiposo/fisiología , Metabolismo Energético , Ballena Minke/fisiología , Tejido Adiposo/anatomía & histología , Animales , Femenino , Islandia , Masculino , Ballena Minke/anatomía & histología , Modelos Biológicos , Reproducción , Estaciones del AñoRESUMEN
A selection of PCN congeners was analyzed in pooled blubber samples of pilot whale (Globicephala melas), ringed seal (Phoca hispida), minke whale (Balaenoptera acutorostrata), fin whale (Balaenoptera physalus), harbour porpoise (Phocoena phocoena), hooded seal (Cystophora cristata) and Atlantic white-sided dolphin (Lagenorhynchus acutus), covering a time period of more than 20 years (1986-2009). A large geographical area of the North Atlantic and Arctic areas was covered. PCN congeners 48, 52, 53, 66 and 69 were found in the blubber samples between 0.03 and 5.9 ng/g lw. Also PCBs were analyzed in minke whales and fin whales from Iceland and the total PCN content accounted for 0.2% or less of the total non-planar PCB content. No statistically significant trend in contaminant levels could be established for the studied areas. However, in all species except minke whales caught off Norway the lowest ∑PCN concentrations were found in samples from the latest sampling period.