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1.
Artículo en Inglés | MEDLINE | ID: mdl-15994136

RESUMEN

A rapid and sensitive method to determine 8-oxoguanine (8oxoG) and 8-hydroxydeoxyguanosine (8OHdG), biomarkers for oxidative DNA damage, in cerebral cortex microdialysate samples using capillary electrophoresis (CE) with electrochemical detection (CEEC) was developed. Samples were concentrated on-column using pH-mediated stacking for anions. On-column anodic detection was performed with a carbon fiber working electrode and laser-etched decoupler. The method is linear over the expected extracellular concentration range for 8oxoG and 8-OHdG during induced ischemia-reperfusion, with R.S.D. values

Asunto(s)
Biomarcadores/análisis , Corteza Cerebral/química , Desoxiadenosinas/análisis , Guanina/análogos & derivados , Animales , Cromatografía Liquida/métodos , Electroquímica , Electroforesis Capilar/métodos , Femenino , Guanina/análisis , Espectrometría de Masas/métodos , Microdiálisis/métodos , Ratas , Ratas Sprague-Dawley
2.
J Pharm Biomed Anal ; 38(5): 904-9, 2005 Aug 10.
Artículo en Inglés | MEDLINE | ID: mdl-15876508

RESUMEN

The concentration of low molecular weight compounds in tissues can yield valuable information about the metabolic state of an organism. Studies of changes in the metabolic state or metabonomics can reflect disease pathways, drug action, or toxicity. This research aims to develop a new approach, tissue targeted metabonomics. Microdialysis sampling and microcoil NMR analysis are employed to compare basal and ischemic metabolic states of various tissues (blood, brain, and heart) of Sprague-Dawley rats. Microdialysis sampling is localized, making the metabolic profile tissue specific. Coupling to NMR analysis is highly advantageous, because a complete metabolic profile is obtained in a single spectrum. However, small sample volumes and low analyte concentrations make analysis of microdialysis samples challenging. Microcoil NMR uses low sample volumes and has improved mass sensitivity, relative to standard 5 mm probes. The coupling of these techniques is a potentially powerful tool for metabonomics analysis.


Asunto(s)
Encéfalo/metabolismo , Microdiálisis/métodos , Isquemia Miocárdica/metabolismo , Miocardio/metabolismo , Resonancia Magnética Nuclear Biomolecular/métodos , Animales , Isquemia Miocárdica/sangre , Especificidad de Órganos , Ratas , Ratas Sprague-Dawley
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