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In this study, hydroxyapatite (HAp) was synthesized from natural biowaste materials, specifically mussel shells, and combined with chitosan (CS) and gentamicin sulfate antibiotic (GA) using an in-situ method. The resulting composite material, designated HAp/CS-GA, has its physicochemical and structural properties characterized by Fourier transform infrared spectroscopy (FTIR) analysis. The structure was confirmed by X-ray diffraction (XRD) analysis. Additionally, field emission scanning electron microscopy (FE-SEM) equipped with the energy dispersive X-ray spectroscopic (EDX) technique was used to determine the surface topography and main components. The composite of HAp/CS-GA was analyzed using a drug release profile by UV-visible spectroscopy (UV-Vis). The fabricated composites antimicrobial behavior was examined against bone infection-causing Gram-positive and Gram-negative bacteria, showing potential activity against Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus compared to Escherichia coli, respectively. Simultaneously, the cytotoxicity of the composite was evaluated by MTT assay using an MG-63 osteoblast-like cell line that exhibited no toxicity in the prepared composite. After a 24 h incubation period, the MG-63 cells on the HAp/CS-GA composite showed good proliferation, according to Hoechst 33258 fluorescence staining results. The results suggested that the composite had excellent biocompatibility and antibacterial activity and enhanced the osteoblast cell proliferation. Therefore, the designed HAp/CS-GA composite would be a promising candidate for bone tissue engineering.
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The release of industrial wastewater has adverse effects on both aquatic ecosystems and the environment. Discharging untreated organic dyes into aquatic environments significantly amplifies pollution levels in these ecosystems. Ensuring the appropriate disposal of organic colorants and their derivatives before introducing them into wastewater streams is essential to prevent environmental contamination. This study aimed to develop an eco-friendly and sustainable approach to synthesize a chitosan-functionalized silver (Ag) nanocomposite using Solanum trilobatum for color pollutant mitigation. The synthesized CS-Ag nanocomposite was analyzed using various techniques such as UV-visible, FTIR, TEM, and EDS. TEM analysis revealed that the CS-Ag nanocomposite had a spherical nanostructure, with diameters ranging from 17.4 to 43.9 nm. These nanocomposites were tested under visible light irradiation to analyze their photocatalytic character against Congo red (CR). The nanocomposite exhibited a remarkable dye removal efficiency of over 93.6% within 105 min under irradiation. In the experimental recycling study, the CS-Ag nanocomposites demonstrated remarkable stability and reusability. Furthermore, the CS-Ag nanocomposite exhibited promising inhibition activity against bacterial pathogens. Our research revealed that the synthesized nanocomposite has the potential to act as a highly effective photocatalyst and bactericidal agent in various industrial and clinical applications.
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Antibacterianos , Quitosano , Colorantes , Nanocompuestos , Plata , Contaminantes Químicos del Agua , Quitosano/química , Nanocompuestos/química , Plata/química , Antibacterianos/farmacología , Antibacterianos/química , Colorantes/química , Contaminantes Químicos del Agua/química , Catálisis , Rojo Congo/química , Restauración y Remediación Ambiental/métodos , Aguas Residuales/química , LuzRESUMEN
Green nanotechnology is an emerging field of science that focuses on the production of nanoparticles by living cells through biological pathways. This topic plays an extremely imperative responsibility in various fields, including pharmaceuticals, nuclear energy, fuel and energy, electronics, and bioengineering. Biological processes by green synthesis tools are more suitable to develop nanoparticles ranging from 1 to 100 nm compared to other related methods, owing to their safety, eco-friendliness, non-toxicity, and cost-effectiveness. In particular, the metal nanoparticles are synthesized by top-down and bottom-up approaches through various techniques like physical, chemical, and biological methods. Their characterization is very vital and the confirmation of nanoparticle traits is done by various instrumentation analyses such as UV-Vis spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), atomic force microscopy (AFM), annular dark-field imaging (HAADF), and intracranial pressure (ICP). In this review, we provide especially information on green synthesized metal nanoparticles, which are helpful to improve biomedical and environmental applications. In particular, the methods and conditions of plant-based synthesis, characterization techniques, and applications of green silver, gold, iron, selenium, and copper nanoparticles are overviewed.
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Nanopartículas del Metal , Extractos Vegetales , Espectroscopía Infrarroja por Transformada de Fourier , Extractos Vegetales/química , Plata/química , Nanotecnología , Nanopartículas del Metal/química , Tecnología Química Verde/métodos , Difracción de Rayos X , AntibacterianosRESUMEN
MnO2 nanoparticles have a wide range of applications, including catalytic abilities due to their oxygen reduction potential. Industrial processes and the burning of organic materials released PAHs into the biosphere which have adverse effects on living organisms when continually exposed. In this study, MnO2 nanoparticles were synthesized chemically using sodium thiosulphate as reducing agent. MnO2 nanoparticles were characterized using UV-visible adsorption spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). A X-Ray Diffraction Spectrophotometer (XRD), a Scanning Electron Microscopy - Energy Dispersive X-Ray Analyzer (SEM-EDAX), and Dynamic Light Scattering (DLS) were used to identify the crystalline nature and particle size of the fabricated MnO2 nanoparticles. Batch adsorption studies were conducted to identify the optimal conditions for better benzene and pyrene adsorption from aqueous solution using MnO2 nanoparticles. They are also effective in degrading benzene and pyrene by batch adsorption as determined by their adsorption isotherms and kinetics.
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Nanoparticles synthesis from green chemistry method is gaining a lot of attention due to their non-toxic, low cost and facile. In this study, a copper oxide nanoparticle (CuO NPs) was synthesized using Sida cordifolia aqueous leaf extract and incorporated chitosan biomolecules to potential enhancing of biological properties. The CuO NPs and chitosan (CS) embedded nanocomposite was noted as CuO-CS nanocomposite, its was physicochemical characterized by using of UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray Diffraction (XRD) and Field emission scanning electron microscopy (FE-SEM) with Energy dispersive X-ray (EDX) analysis. Bio-functionalized CuO-CS nanocomposite was performed antibacterial efficiency against both Gram positive (Staphylococcus aureus, Bacillus subtilis) and Gram negative (Salmonella typhi, Escherichia coli) bacteria through the Mueller Hinton agar (MHA) well diffusion techniques. The highest bactericidal activity was revealed Gram positive of B. subtilis and Gram negative of S. typhi bacteria, respectively. Further, the cytotoxicity effect of biosynthesized nanocomposite was an examined against human breast cancer MDA-MB-231 and lung cancer A549 cell lines. The half maximal inhibitory concentration is showed at 2 µg/mL for MDA-MB-231and 4 µg/mL was A549 cells. Live/dead cells were detected by fluorescence microscopic observation at the IC50 concentration. In furthermore, bio-functionalized CuO-CS nanocomposite was performed photocatatlytic dye degradation against for industrial dyes of crystal violet (CV) and malachite green (MG). From the results, synergic bio-functionalized CuO-CS nanocomposite was suggested potential suitable for biomedical applications as well as industrial wastewater treatment.
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Quitosano , Neoplasias Pulmonares , Nanopartículas del Metal , Nanocompuestos , Humanos , Quitosano/química , Cobre/química , Espectroscopía Infrarroja por Transformada de Fourier , Antibacterianos/farmacología , Antibacterianos/química , Nanocompuestos/química , Bacterias , Neoplasias Pulmonares/tratamiento farmacológico , Agua , Línea Celular , Pruebas de Sensibilidad Microbiana , Difracción de Rayos X , Nanopartículas del Metal/toxicidad , Nanopartículas del Metal/químicaRESUMEN
Textile industry is the major backbone of economy of the developing countries. The major problems associated in the textile factories are release of undesired dye effluents, which is a potential pollution risk for human health as well as the environmental aquatic system. The objective of this study was fabrication of a novel composite to treat textile industry effluents in an ecofriendly manner. In this context, hydroxyapatite (HAp) was derived from the mussel shell biowaste and fabricated with chitosan-sodium alginate through the in-situ method. The prepared HAp/CS-SA composite was physicochemically characterized by using Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX) and High Resolution-Transmission Electron Microscope (HR-TEM). The photocatalytic activity of the HAp/CS-SA composite was evaluated by using Congo red (CR), Malachite green (MG) and Methylene blue (MB) as model dyes for degradation in aqueous solutions under solar irradiation. The degradation rate was recorded as CR (88%), MG (81%) and MB (93%) respectively within 75 min of irradiation. The degraded end products were subjected to toxicity assessment by evaluating the phytotoxicity on seed germination of Vigna radiata plant in pot study. The degraded end products were also tested for toxic inhibition of E. coli and P. aeruginosa by agar well diffusion method. The prepared HAp/CS-SA composite had an enhanced photocatalytic activity due to the presence of natural biopolymers and their biological properties. HAp/CS-SA composite showed potential dye degradation properties and it could be effective for dye removal from industrial wastewaters.
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Quitosano , Colorantes , Agar/farmacología , Alginatos/farmacología , Quitosano/química , Colorantes/química , Colorantes/toxicidad , Rojo Congo/farmacología , Durapatita/farmacología , Escherichia coli , Humanos , Azul de Metileno/química , Azul de Metileno/farmacología , Aguas Residuales , AguaRESUMEN
In currently, biosynthesis of copper oxide nanoparticles (CuO NPs) are most widely used numerous in biological applications such as biosensor, energy, medicine, agriculture, environmental and industrial wastewater treatment. The hierarchical CuO NPs was synthesized via green chemistry method by using of Abutilon indicum (A. indicum) leaf extract, its nontoxic, facile and low-cost approaches. Biogenic synthesized CuO NPs was characterized by using a UV-visible absorption spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Field mission scanning electron microscopy (FE-SEM) with Energy-dispersive X-ray spectroscopy (EDX) analysis. The synthesized CuO NPs was performed antibacterial activity against human pathogenic organisms of both Gram negative (Escherichia coli and Salmonella typhi) and Gram positive (Bacillus subtilis and Staphylococcus aureus) bacteria by using agar well diffusion method. Biological synthesized CuO NPs was showed potential bactericidal activity against Gram positive bacteria of B. subtilis than compared to Gram negative bacteria of E. coli. The cytotoxic effect of A. indicum mediated synthesized CuO NPs was evaluated against to human lung A549 and breast MDA-MB-231cancer cell lines by determined using of MTT assay. In furthermore, photocatalytic dye degradation was performed that synthesized CuO NPs have effectively removed 78% of malachite green dye molecule. Our investigation results suggested that the green synthesized CuO NPs potential biological activity of antibacterial activity against Gram positive bacterial, anticancer activity was effectively against MDA-MB-231cancer cell line and good dye degradation was exhibited in malachite green. The A. indicum aqueous leaf extract mediated synthesized CuO NPs has strongly suggested promising nano-biomaterials for fabrication of biomedical applications.
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Neoplasias de la Mama , Malvaceae , Nanopartículas del Metal , Agar/farmacología , Antibacterianos/química , Neoplasias de la Mama/tratamiento farmacológico , Cobre/química , Cobre/farmacología , Escherichia coli , Femenino , Bacterias Grampositivas , Humanos , Pulmón , Nanopartículas del Metal/química , Pruebas de Sensibilidad Microbiana , Óxidos , Extractos Vegetales/química , Extractos Vegetales/farmacología , Colorantes de Rosanilina , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
Semiconductor photocatalysts are efficient degraders of organic and inorganic waste water pollutants. Herein, we synthesized nickel-titanium dioxide (Ni-TiO2) nanoflakes using Mukia maderaspatana leafs with the aim of analyzing their photocatalytic degradation potential. Morphological analyses revealed that the nanoflakes were highly agglomerated with an average size of 100 nm. Further, elemental analysis confirmed the presence of Ti, O, and Ni, whereas Fourier transform infrared spectroscopy and X-ray diffraction established the presence of TiO2 and NiO. We found that photocatalytic degradation of congo red under UV illumination increased with increasing incubation period, demonstrating that Ni-TiO2 nanoflakes can be used as optimal photocatalysts for the degradation of dyes in waste water.
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Rojo Congo , Níquel , Catálisis , Colorantes , Extractos Vegetales , Titanio , Difracción de Rayos XRESUMEN
In the present study, the biogenic synthesis of an ecofriendly and non-toxic chitosan (CS) coated copper oxide NPs (CS-CuO nanocomposite) using Psidium guajava aqueous leaf extract. The biogenic synthesized CS-CuO nanocomposite was characterized by using UV-vis spectroscopy analysis (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FE-SEM-EDS), Dynamic light scattering (DLS), and Transmission electron microscopy (TEM). The prepared CS-CuO nanocomposite was evaluated for antibacterial activity by agar well diffusion method as well as minimum inhibitory concentrations (MIC) were assessed against both Gram-positive Streptococcus pneumoniae, Staphylococcus epidermidis and Gram-negative Escherichia coli, Proteus mirabilis with good inhibition effects on Gram-negative bacteria than the Gram-positive bacteria. The interaction of the CS-CuO nanocomposite with the bacterial membrane was visually observed by confocal laser scanning microscopy and the live/dead cells were differentiated by treatment with acridine orange and ethidium bromide dyes.
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Antibacterianos , Quitosano , Cobre , Nanocompuestos , Extractos Vegetales/química , Psidium , Contaminantes Químicos del Agua/química , Antibacterianos/administración & dosificación , Antibacterianos/química , Antibacterianos/efectos de la radiación , Compuestos Azo/química , Bacterias/efectos de los fármacos , Bacterias/crecimiento & desarrollo , Quitosano/administración & dosificación , Quitosano/química , Quitosano/efectos de la radiación , Colorantes/química , Rojo Congo/química , Cobre/administración & dosificación , Cobre/química , Cobre/efectos de la radiación , Azul de Metileno/química , Pruebas de Sensibilidad Microbiana , Nanocompuestos/administración & dosificación , Nanocompuestos/química , Nanocompuestos/efectos de la radiación , Fotólisis , Hojas de la Planta , Soluciones , Luz Solar , Purificación del Agua/métodosRESUMEN
Hydroxyapatite (HAp) is a bioactive and biocompatible material possessing osteoconductive properties used widely in the biomedical sector. In the present study, synthesis of hydroxyapatite (HAp) using a Klebsiella pneumoniae SM24 (phosphate solubilizing bacteria) isolated from the slaughterhouse. HAp synthesized using biological source showed efficient and positive enzymatic activity in the National Botanical Research Institute Phosphate Medium (NBRIP). Characterization of HAp using FTIR revealed the presence of phosphate group hydroxyapatite and XRD spectra showed polycrystalline nature. The morphological characterization of HAp using FESEM revealed the mesoporous structure and EDX spectrum indicated presence of Ca and P as the major components. In addition, a new bone composite was prepared using the synthesized HAp, Gelatine (G), Chitosan (C), Fibrin (F) and Bone ash (HApGCF) using Simulated Body Fluid (SBF) solution. The confirmation of chemical and structural characteristics of HApGCF bone composite was achieved using FTIR, XRD and SEM analyses. The HApGCF bone composite was tested over osteoblast MG-63 cells showing effective biocompatibility and osteoblast attachment on the composite surface. Therefore, the present report proposes the in vitro application of HApGCF bone composite as a replacement for major bone damage and injury in a biocompatible and non-toxic way.
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Materiales Biocompatibles/síntesis química , Huesos , Quitosano/química , Durapatita/química , Fibrina/química , Gelatina/química , Minerales/química , Ingeniería de Tejidos , Animales , Materiales Biocompatibles/química , Línea Celular , Proliferación Celular , Análisis Espectral , Termogravimetría , Andamios del TejidoRESUMEN
Green synthesis of nanoparticles is one of the promising, ecofriendly and safer methods. Utilizing plant sources as reducing agents will replace the use of toxic chemicals for nanoparticle synthesis. In the present study FeONPs were synthesized using Ruellia tuberosa (RT) leaf aqueous extract, further characterization of FeONPs was performed using UV-vis spectroscopy analysis showing visible peak at 405â¯nm. The Fourier transform infrared spectroscopy (FTIR) proved the presence of Fe metallic ions. The structural characteristic using Field emission scanning electron microscopy with energy dispersive x-ray spectroscopy (FESEM-EDX) and Transmission electron microscopy (TEM) analysis revealed hexagonal nanorods with agglomeration. Dynamic light scattering (DLS) calculated the average size of FeONPs around 52.78â¯nm and differential scanning colorimetry (DSC) proved the stability of FeONPs till higher temperature of 165.52⯰C. As an application part, the synthesized FeONPs showed potential antibacterial activity as individual and incorporating material over cotton fabrics against Gram negative and Gram positive pathogens. FeONPs showed higher antibacterial activity against Escherichia coli, Klebsiella pneumoniae and lesser antibacterial activity against Staphylococcus aureus. The photocatalytic ability of the synthesized FeONPs was demonstrated by the degrading crystal violet dye under solar irradiation upto 80%. Thus, FeONPs synthesized using Ruellia tuberosa could play a vital role in killing the bacterial pathogens and degrading dye for the bioremediation of wastewater from industrial and domestic sources.
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Acanthaceae/química , Antibacterianos/farmacología , Compuestos Férricos/síntesis química , Nanopartículas del Metal/química , Fotólisis/efectos de los fármacos , Extractos Vegetales/química , Biodegradación Ambiental , Catálisis/efectos de los fármacos , Colorantes/química , Escherichia coli/efectos de los fármacos , Tecnología Química Verde , Klebsiella pneumoniae/efectos de los fármacos , Aguas Residuales/químicaRESUMEN
Growing concerns over the toxicity of metallic nanoparticles synthesized using physical and chemical techniques seems to be a major hurdle for researchers. Green synthesis of nanoparticles is one of the promising, ecofriendly and safer methods. Utilizing plant sources as reducing agents will replace the use of toxic chemicals for nanoparticle synthesis. Among the various nanoparticles, copper has been theoretically and practically proved for its antimicrobial properties. However, to reduce the risk of copper toxicity, Ruellia tuberosa (R. tuberosa) aqueous extract is used for the synthesis of CuONPs in the present study. Nonetheless, till date no work has been reported on the use of R. tuberosa aqueous extract for the synthesis of CuONPs. In the present study, aqueous extract of R. tuberosa has been used for the synthesis of CuONPs. The synthesis of CuONPs was confirmed by the absorption peak at 327â¯nm representing the nanorods with an average size of 83.23â¯nm. Further, the CuONPs revealed antimicrobial effects against clinical pathogens such as Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Embedding CuONPs on cotton fabrics showed bactericidal activity against the bacterial pathogens. In addition, the photocatalytic property of the CuONPs was divulged by their crystal violet (CV) dye degradation potential. Thus, the green synthesized CuONPs using R. tuberosa could provide a remedy against bacterial pathogens in hospital and industrial environments.
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Cobre/química , Tecnología Química Verde/métodos , Nanopartículas del Metal/química , Textiles , Acanthaceae/química , Antibacterianos/farmacología , Bacterias/efectos de los fármacos , Colorantes/química , Violeta de Genciana , Procesos Fotoquímicos , Extractos Vegetales/químicaRESUMEN
Textile industry is a major sector providing global financial and employment support to different countries of the world. The major problems of the textile industry are dirt and microbial contaminants affecting the quality of cotton fabrics. Recently, nanoparticles such as silver, chitosan, silicon dioxide, titanium dioxide and zinc oxide have gained attraction in textile industries to avoid the contamination of fabrics through microbes. The necessity to develop an ecofriendly, efficient and cost effective method for the synthesis of nanoparticles is under the radar. Plant extracts serve as potential reducing and coating agents due to the presence of bioactive molecules such as phenols, lipids, carbohydrates, enzymes, protein molecules etc., which endow effective antimicrobial activity to the nanoparticles. In the present study, biological synthesis of Copper oxide nanoparticles (CuONPs) was performed using S. acuta leaf extract. CuONPs were synthesized and characterized using UV-vis, FTIR, SEM and TEM analyses. The antimicrobial property of CuONPs was tested against Gram negative (Escherichia coli and Proteus vulgaris) and Gram positive (Staphylococcus aureus) pathogens, which showed zones of inhibition at different concentrations. As the final part of the study, CuONPs were coated over cotton fabrics showing longer stability, which prevented the growth of infectious pathogens. Apart from the antimicrobial activity, CuONPs synthesized using S. acuta possessed effective photocatalytic activity against commercial dyes.
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Antibacterianos/química , Cobre/química , Fibra de Algodón , Malvaceae/química , Nanopartículas del Metal/química , Extractos Vegetales/química , Antibacterianos/farmacología , Compuestos Azo/química , Catálisis , Pruebas Antimicrobianas de Difusión por Disco , Violeta de Genciana/química , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Tecnología Química Verde , Luz , Malvaceae/metabolismo , Nanopartículas del Metal/toxicidad , Microscopía Electrónica de Transmisión , Fotólisis/efectos de los fármacos , Fotólisis/efectos de la radiación , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azadirachta indica. The antibacterial potential of the methanol leaf extract of the studied species was tested against Escherichia coli, Pseudomonas aeruginosa, Klebsiellap neumoniae, Streptococcus pyogens, Staphylococcus aureus using by agar well diffusion method. The higher zone of inhibition (16mm) was observed against the bacterium Pseudomonas aeruginosa at 100µl concentration of methanol leaf extract. Preliminary phytochemical analysis of studied species shows that presence of phytochemical compounds like steroids, phenolic compounds and flavonoids. GC-MS analysis confirms the occurrence of 20 different compounds in the methanol leaf extract of the both studied species.