RESUMEN
A novel approach was developed for the construction of biofilms with defined internal architecture using AC electrokinetics and flocculation. Artificial structured microbial consortia (ASMC) consisting of localized layered microcolonies of different cell types were formed by sequentially attracting different cell types to high field regions near microelectrodes using dielectrophoresis. Stabilization of the microbial consortia on the electrode surface was achieved by crosslinking the cells using the flocculant polyethyleneimine (PEI). Consortia of Escherichia coli, Micrococcus luteus, and Saccharomyces cerevisiae were made as model systems. Also, more natural consortia were made of the bacteria Pseudomonas putida, Clavibacter michiganense, and Methylobacterium mesophilum, which are found together in consortia during biodegradation of metal-cutting waste fluids.
Asunto(s)
Adhesión Bacteriana/efectos de los fármacos , Biopelículas/crecimiento & desarrollo , Técnicas de Cultivo de Célula/métodos , Electroforesis/métodos , Polietileneimina/farmacología , Actinomycetales/citología , Actinomycetales/efectos de los fármacos , Actinomycetales/fisiología , Adhesión Bacteriana/efectos de la radiación , Biopelículas/efectos de los fármacos , Biopelículas/efectos de la radiación , Relación Dosis-Respuesta a Droga , Campos Electromagnéticos , Escherichia coli/citología , Escherichia coli/efectos de los fármacos , Escherichia coli/fisiología , Floculación , Methylobacterium/citología , Methylobacterium/efectos de los fármacos , Methylobacterium/fisiología , Micrococcus luteus/citología , Micrococcus luteus/efectos de los fármacos , Micrococcus luteus/fisiología , Pseudomonas putida/citología , Pseudomonas putida/efectos de los fármacos , Pseudomonas putida/fisiología , Saccharomyces cerevisiae/citología , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/fisiologíaRESUMEN
A method was developed for the measurement of the bacterial particle conductivity, based on the measurement of the conductivity of a bacterial cell suspension sigma(s) and the suspending medium sigma(m). A line plotted through sigma(s) - sigma(m) versus sigma(m) crosses the x-axis at sigma(m) = sigma(p), independent of the bacterial cell concentration. The method does not require anything more complex than a centrifuge and a conductivity meter. Knowledge of the bacterial particle conductivity is of importance in, for example, the dielectrophoretic separation, manipulation and trapping of bacterial cells, as well as the study of their physiological state.
