Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
Más filtros












Base de datos
Intervalo de año de publicación
1.
Fitoterapia ; 135: 64-72, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31004693

RESUMEN

Four new pterocarpans (6aR,11aR)-6a,11a-dihydrolespedezol A2 (2), (6aR,11aR)-2-isoprenyl-6a,11a-dihydrolespedezol A2 (3), (6aR,11aR,3'R)-6a,11a-dihydrolespedezol A3 (4), (6aR,11aR,3'S)-6a,11a-dihydrolespedezol A3 (5) and one new stilbenoid with 1,2-diketone fragment named bicoloketone (6) along with one previously known pterocarpen lespedezol A2 (1) have been isolated from Lespedeza bicolor stem bark using multistage column chromatography on polyamide and silica gel. The structures of the isolated polyphenolic compounds were determined by spectroscopic methods. The absolute configurations of 4 and 5 were determined by comparison of their electronic circular dichroism (ECD) spectra obtained experimentally and the spectra calculated using time-dependent density functional theory (TDDFT). The isolated compounds exhibited a moderate DPPH scavenging effect and ferric reducing power compared to the reference antioxidant quercetin. The cytotoxicity of compounds against three human cancer cell lines, HTB-19, Kyse-30, and HEPG-2, and two normal cell lines, RPE-1 and HEK-293, was tested using the MTT assay. Compound 3 showed the strongest cytotoxic activity against all cell lines (IC50 6.0-19.1 µM) compared with the positive control cisplatin. The other tested compounds possessed moderate cytotoxic activity against cancer cells.


Asunto(s)
Antioxidantes/farmacología , Lespedeza/química , Polifenoles/farmacología , Pterocarpanos/farmacología , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Estructura Molecular , Polifenoles/química , Polifenoles/aislamiento & purificación , Pterocarpanos/química , Pterocarpanos/aislamiento & purificación
2.
Nat Prod Commun ; 8(10): 1419-20, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24354190

RESUMEN

A callus culture of Iris pseudacorus L. (Iridaceae) was established from plant leaves using a modified Murashige and Skoog medium. A derivative of cinnamic acid (lavandoside) (1), a neolignan (dehydrodiconiferyl alcohol-4-O-beta-D-glucopyranoside) (2) as well as three isoflavonoids, tectoridin (3), tectorigenin (4), and iristectorigenin A (5) were isolated from the callus culture. Under normal conditions, the calli accumulated 0.4% DW of polyphenols. The addition of phenylalanine to a concentration of 1 mM resulted in a 1.5-fold increase in isoflavonoid production, allowing the accumulation of 0.69% of polyphenols in the callus dry weight. Tectorigenin, a promising chemotherapeutic and chemopreventive agent for the treatment of carcinomas, was produced in I. pseudacorus calli in high quantities (0.3% DW).


Asunto(s)
Género Iris/química , Isoflavonas/aislamiento & purificación , Polifenoles/aislamiento & purificación , Células Cultivadas , Técnicas de Cultivo , Lignanos/aislamiento & purificación
3.
J Plant Physiol ; 166(11): 1194-206, 2009 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-19285358

RESUMEN

It has been established that transformation of Vitis amurensis callus culture with the plant oncogene rolB of Agrobacterium rhizogenes results in a high level of resveratrol production in the transformed culture. In the present report, we investigated two rolB transgenic V. amurensis cell cultures with different levels of rolB expression and resveratrol production. We examined whether the calcium ion flux and later steps of the calcium-mediated signal transduction pathway play a role in resveratrol biosynthesis in the rolB transgenic cultures. It has been shown that the calcium channel blockers, LaCl(3), verapamil, and niflumic acid, significantly reduced the accumulation of resveratrol in the rolB transgenic cultures. The number of the calcium-dependent protein kinase (CDPK) transcript variants and abundance of some of the transcripts were considerably altered in the rolB transgenic cell cultures, as revealed by frequency analysis of RT-PCR products and real-time PCR. Some unusual CDPK transcripts with deletions and insertions in the kinase domain were isolated from cDNA probes of rolB-transformed cells. These results suggest that active resveratrol biosynthesis in rolB transgenic cultures of V. amurensis is Ca2+ dependent. We propose that the rolB gene has an important role in regulation of calcium-dependent transduction pathways in transformed cells.


Asunto(s)
Proteínas Bacterianas/genética , Regulación de la Expresión Génica de las Plantas , Plantas Modificadas Genéticamente/metabolismo , Proteínas Quinasas/genética , Estilbenos/metabolismo , Vitis/metabolismo , beta-Glucosidasa/genética , Cromatografía Líquida de Alta Presión , Plantas Modificadas Genéticamente/genética , Resveratrol , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Vitis/genética
4.
J Agric Food Chem ; 56(16): 7023-31, 2008 Aug 27.
Artículo en Inglés | MEDLINE | ID: mdl-18671403

RESUMEN

Isoflavonoids, an interesting and restricted group of secondary metabolites of legumes, exhibit estrogenic, antiangiogenic, and anticancer activities and are now popular as dietary supplements. Plant cell cultures that possess an increased ability to synthesize these metabolites were examined. During the investigation, cell cultures of the Far Eastern relict tree Maackia amurensis (Leguminosae) were established. A selection of seed-derived cell aggregates yielded the callus line designated A-18. This culture produces 20 isoflavonoids, namely, the isoflavones genistein, daidzein, formononetin, calycosin, derrone, and pseudobaptigenin and their glycosylated conjugates genistin, 6''-O-malonylgenistin, ononin, 6''-O-malonylononin, daidzin, 3'-methoxydaidzin, 4'-O-beta-D-glucopyranosyldaidzin, 4'-O-beta-D-glucopyranosylgenistin, and 7-O-beta-D-glucopyranosylcalycosin; the pterocarpans maackiain and medicarpin and their glycosylated conjugates 6'-O-malonyl-3-O-beta-D-glucopyranosylmaackiain and 6'-O-malonyl-3-O-beta-D-glucopyranosylmedicarpin; and the new pterocarpan glucoside 6'-O-malonyl-3-O-beta-D-glucopyranosyl-6,6a-dehydromaackiain. These isoflavonoids, possessing a hepatoprotective activity, were stably produced by the A-18 calli for prolonged periods of observation.


Asunto(s)
Isoflavonas/análisis , Maackia/química , Plantones/química , Línea Celular , Cromatografía Líquida de Alta Presión , Glicosilación , Isoflavonas/biosíntesis , Isoflavonas/química , Espectroscopía de Resonancia Magnética , Plantones/citología , Plantones/metabolismo
5.
Biosci Biotechnol Biochem ; 71(5): 1286-93, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17485828

RESUMEN

During an investigation of plant cell cultures that might be useful in the treatment of renal disorders, we established a vigorously-growing E-4 callus culture of Eritrichium sericeum that produced large amounts of caffeic acid metabolites, (-)-rabdosiin (1.8% dry wt) and rosmarinic acid (4.6% dry wt). Elicitation of the calli by methyl jasmonate induced a 38% increase in total polyphenol production. The most efficient method of eliciting (-)-rabdosiin biosynthesis was through the treatment of E-4 calli with cuprum glycerate, which induced an increase in (-)-rabdosiin production of as much as 4.1% dry wt. Oral administration of E-4 callus biomass (100 mg/kg/d for 30 d) to rats with induced Masugi-nephritis caused an increase in diuresis and lowered creatinine excretion and proteinuria levels as compared with Masugi-nephritis untreated rats. While all of the Masugi-nephritis untreated rats began to suffer, near a quarter of the E-4 treated rats remained in good health. This result indicates that the E-4 culture has the potential to alleviate the symptoms associated with nephritis.


Asunto(s)
Boraginaceae/citología , Boraginaceae/metabolismo , Ácidos Cafeicos/metabolismo , Cinamatos/metabolismo , Depsidos/metabolismo , Nefritis/tratamiento farmacológico , Fitoterapia , Acetatos/farmacología , Animales , Biomasa , Boraginaceae/química , Boraginaceae/efectos de los fármacos , Ácidos Cafeicos/química , Células Cultivadas , Cinamatos/química , Cobre/farmacología , Creatinina/metabolismo , Ciclopentanos/farmacología , Depsidos/química , Diuresis/efectos de los fármacos , Ácidos Glicéricos/farmacología , Cinética , Lignanos , Estructura Molecular , Nefritis/inducido químicamente , Nefritis/clasificación , Nefritis/patología , Nefritis/fisiopatología , Oxilipinas , Reguladores del Crecimiento de las Plantas/farmacología , Proteinuria/tratamiento farmacológico , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Ácido Rosmarínico
6.
Planta Med ; 71(5): 446-51, 2005 May.
Artículo en Inglés | MEDLINE | ID: mdl-15931584

RESUMEN

Eritrichium sericeum (Boraginaceae) callus and root cultures were established and analyzed for caffeic acid metabolite (CAM) production. Two substances, (-)-rabdosiin and rosmarinic acid, were identified as main CAMs produced by these cultures. The E. sericeum Er-1 root culture accumulated up to 1.5 % and 4.5 % DW of (-)-rabdosiin and rosmarinic acid, respectively. Rabdosiin in the Lithospermum erythrorhizon callus cultures was produced exclusively as the (+)-enantiomer while in both Eritrichium cultures it occurred as the (-)-enantiomer. The E. sericeum Er-1 culture accumulated 3-fold higher levels of CAMs than the L. erythrorhizon culture. A new compound, named eritrichin, was isolated from the cultured E. sericeum cells. The structure of this compound was established as (2R)-3-(3,4-dihydroxyphenyl)-2-[4-(3,4-dihydroxyphenyl)-6,7-dihydroxy-2-naphthoyloxy]propanoic acid on the basis of spectral data.


Asunto(s)
Boraginaceae/metabolismo , Ácidos Cafeicos/metabolismo , Fitoterapia , Boraginaceae/química , Ácidos Cafeicos/química , Células Cultivadas , Humanos , Raíces de Plantas/química , Raíces de Plantas/metabolismo , Semillas/química , Semillas/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...