RESUMEN
This study investigated the geographic distribution and prevalence of antibodies to California and Bunyamwera serogroup viruses in Native populations of Alaska, and demographic and ecologic risk factors associated with exposure. Sera (n = 1,635) from 18 communities were screened using an ELISA. All age groups were tested for antibodies to Jamestown Canyon (JC), Inkoo (INK), snowshoe hare (SSH), and Northway (NOR) viruses; persons > or = 45 years old (n = 90) from six communities were additionally tested for antibodies to Tahyna (TAH), Batai (BAT), Cache Valley (CV), and Sindbis (SIN) viruses. Thirty free-ranging mammals were tested by a plaque reduction neutralization test (PRNT) for antibodies to all eight viruses and to Getah (GET) virus. In Natives, overall antibody prevalence was 24.9% (JC = 17.6%, monotypic JC = 6.5%, INK = 11.1%, monotypic INK = 0.6%, SSH = 6.8%, monotypic SSH = 3.5%, and NOR = 6.2%). Five TAH, CV, and BAT virus exposures may be serologic cross-reactions, and no SIN virus antibodies were detected. Sindbis-like virus antibodies were found in 30% of the mammals. Most mammals had antibodies to NOR (83.3%) and California serogroup (70.0%) viruses; no GET virus exposures were found. Significant risk factors for human bunyavirus exposures were age group, ethnic-linguistic group, biotic province, climate zone, terrestrial vegetation, and presence of some ungulates and small mammals in communities. Sex was not a significant risk factor.
Asunto(s)
Virus Bunyamwera/inmunología , Infecciones por Bunyaviridae/epidemiología , Virus de la Encefalitis de California/inmunología , Encefalitis de California/epidemiología , Indígenas Norteamericanos , Adolescente , Adulto , Anciano , Alaska/epidemiología , Animales , Anticuerpos Antivirales/sangre , Niño , Preescolar , Ecología , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Mamíferos , Persona de Mediana Edad , Prevalencia , Factores de Riesgo , Estudios SeroepidemiológicosRESUMEN
The secretion, morphology, and chemical composition of the peritrophic envelope were studied in the phlebotomine sand fly, Lutzomyia spinicrassa Morales, OsornoMesa, Osorno & Hoyos, a suspected vector of Leishmania braziliensis in Colombia and Venezuela. Viewed under light microscopy, the envelope matured rapidly and could be dissected from the blood bolus as early as 12 h and until 36 h after feeding; subsequently it began to degrade. The envelope was initially a closed sac around the blood meal, but opened posteriorly in most flies by 6 h. The posterior opening may facilitate the migration and establishment of Le. braziliensis in the hindgut. Secretion of envelope precursors was from the entire midgut epithelium. Electron microscopy revealed that electron-dense precursor material (possibly chitin) was present, bathing the microvilli during the first 12 h after blood feeding. This secretion appeared to originate from the bases of the microvilli. From 1 to 36 h, an electron-lucid precursor material (possibly protein) was secreted from the entire length of microvilli and from their bases. Both precursors appeared to be formed at the epithelial surface, not associated with secretory vesicles. The envelope developed rapidly from precursor material, and by 6 h a defined electron-lucid structure was present above the microvilli. Most mature envelopes (12-36 h) were 0.5-2.1 microns thick, multilayered, wholly electron-lucid, and composed of microfibrils and granules. Electron-dense components were seen in some envelopes at 24-36 h. An anterior hyaline plug was present from 12 to 36 h. Envelopes were composed of chitin, protein, and glycoprotein, based on chemical and histochemical tests. The likely presence of several amino acids (lysine, aspartic acid, and glutamic acid) that may cross-link chitin and protein was demonstrated by a positive ninhydrin-Schiff test. This study constitutes the first ultrastructural investigation of peritrophic envelope development by a New World sand fly.
Asunto(s)
Psychodidae/anatomía & histología , Animales , Cricetinae , Digestión , Sistema Digestivo/anatomía & histología , Sistema Digestivo/metabolismo , Sistema Digestivo/ultraestructura , Femenino , Mesocricetus , Microscopía Electrónica , Psychodidae/química , Psychodidae/metabolismoRESUMEN
A histologic technique was used to detect multiple hamster blood meals taken by Phlebotomus duboscqi Neveu-Lemaire during a 5-d period. Forty-eight flies were fed two or three blood meals separated by 48, 72, or 120 h and sampled immediately; multiple meals were detected in 27 flies (56%). Double meals separated by 72 h within a single gonotrophic cycle were documented in 11/19 (58%) flies; double meals separated by 120 h were detected in only 4/17 (24%) flies. Triple blood meals taken at 0, 72, and 120 h were detected in 5/12 (42%) flies; all of these flies contained the second and third meals. Early blood meals were detected clearly within later blood meals as a delimited body of dark digested blood, heme (sometimes also with pink undigested blood), the presence of an associated pale pink-staining peritrophic plug, the presence and appearance of the peritrophic membrane surrounding the meals, and a physical space between meals; the first two characteristics were the most important. Development of the ovarian follicles including apparent dilatations was also observable using this histologic technique. The results of this study indicate that the rate of multiple feeding can be determined using histology. The technique would be useful in evaluating the blood feeding frequency of field-caught sand flies in endemic areas of leishmaniasis, bartonellosis, and phleboviruses.
Asunto(s)
Alimentación Animal , Sangre , Conducta Alimentaria , Phlebotomus/fisiología , Animales , Cricetinae , Femenino , Hemo/análisis , Folículo Ovárico/citología , Ovario/citología , Phlebotomus/citología , Factores de TiempoRESUMEN
The development of Leishmania major Yakimoff & Schokhor in the New World sand fly Lutzomyia longipalpis (Lutz & Neiva) was examined by light and electron microscopy. In this unnatural host, parasites differentiated into 10 typical morphological forms, multiplied at three sites, migrated anteriorly and established in the foregut, and attached to gut surfaces. In the blood meal, amastigotes divided and transformed into two successive dividing, stumpy promastigote stages. Elongate nectomonad promastigotes developed from stumpy forms and subsequently rounded up in some flies into paramastigotes and opisthomastigotes. Differentiation into round opisthomastigotes and the apparent fusion of paramastigotes in the blood meal were novel observations in this study. Three nectomonad promastigotes--elongate, short, and metacyclic--were free-swimming in the midgut lumen. Elongate nectomonad promastigotes were highly oriented in the midgut, with their flagella embedded between the epithelial microvilli. Short haptomonad promastigotes were the predominant form attached to the intima of the stomodeal valve, whereas pear-shaped haptomonad promastigotes and paramastigotes colonized surfaces of the esophagus and pharynx. Peripylarian attachment of promastigotes and paramastigotes in the pylorus, ileum, and colon was noted in 21% of flies, suggesting that suprapylarian leishmanias have not lost the ability to colonize the hindgut. L. longipalpis was a successful biological host for L. major, allowing complete development of the parasite.
Asunto(s)
Leishmania tropica/crecimiento & desarrollo , Psychodidae/parasitología , Animales , Movimiento Celular , Sistema Digestivo/parasitología , Femenino , Interacciones Huésped-Parásitos , Leishmania tropica/ultraestructura , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica , Morfogénesis , Especificidad de la EspecieRESUMEN
Leishmania differentiation in the gut of phlebotomine sand flies was evaluated based on five light and electron microscopic studies of natural (Leishmania panamensis/Lutzomyia gomezi, Leishmania chagasi/Lutzomyia longipalpis) and unnatural (Leishmania mexicana/Lutzomyia abonnenci, Leishmania panamensis/Phlebotomus papatasi, Leishmania major/Lutzomyia longipalpis) life cycles. In the bloodmeal, transformation of amastigotes into stumpy promastigotes occurred before or during division. Further division in pairs or rosettes resulted in the development of spatulate and/or elongate nectomonad (free-swimming) promastigotes. Elongate, short, and metacyclic nectomonad promastigotes, and nectomonad paramastigotes were present in the midgut lumen. Dividing short promastigotes predominated in the cardia, and appeared to generate metacyclic forms which were observed in three life cycles. Haptomonad (attached) forms of Leishmania panamensis in the hindgut were primarily spatulate promastigotes (natural host) or pear-shaped promastigotes (unnatural host); paramastigotes and dividing forms were rare. At the stomodeal valve, short haptomonad promastigotes predominated in unnatural hosts, while both short and pear-shaped haptomonads were abundant, along with paramastigotes in natural hosts. Haptomonad paramastigotes and pear-shaped promastigotes colonized the esophagus, while paramastigotes predominated in the pharynx. Metacyclics were free-swimming in the lumen of the foregut.
Asunto(s)
Leishmania/crecimiento & desarrollo , Psychodidae/parasitología , Animales , Esófago/parasitología , Humanos , Intestinos/parasitología , Leishmania/ultraestructura , Faringe/parasitologíaRESUMEN
The secretion, morphology, and chemical composition of the peritrophic membrane was studied in the sand fly, Phlebotomus perniciosus Newstead. The membrane was secreted from the entire midgut epithelium. An electron-dense fine granular secretion, possibly chitin, was present along the length of the microvilli immediately until 24 h after feeding. From 12-48 h, an electron-lucid coarse granular component, possibly protein, was also secreted from the microvillar surface. By light microscopy, the mature 36-h membrane characteristically consisted of a dark anterior cap and posterior open ring, with a transparent intervening membrane and anterior plug. Ultrastructure of the fully formed membrane at 24-48 h was highly variable. Undifferentiated membranes appeared as a single electron-lucid layer; differentiated membranes were more complex, sometimes two-layered, containing electron-lucid and -dense fibers and granules. Results of binding to succinylated wheat germ agglutinin, histochemistry, and amino acid analysis indicated that the membrane was composed of chitin, glycoprotein, and protein. Eighteen amino acids were identified in membrane proteins; aspartic-glutamic acids (and amides), serine, glycine, and lysine (45% by weight) may be important in cross-linking membrane components.
Asunto(s)
Insectos Vectores/crecimiento & desarrollo , Phlebotomus/crecimiento & desarrollo , Animales , Cricetinae , Insectos Vectores/ultraestructura , Mesocricetus , Microscopía Electrónica , Phlebotomus/ultraestructuraRESUMEN
The life cycle of Leishmania panamensis in Phlebotomus papatasi was studied to characterize barriers limiting parasite colonization, differentiation, migration, and attachment in an unnatural sand fly host. The insects were fed a suspension of L. panamensis-infected macrophages and human erythrocytes, and were examined up to 16 days post-infection by light and electron microscopy. Histologic examination of 401 flies showed the peritrophic membrane to be the first important barrier to parasite establishment in the gut lumen. In most flies, parasites were unable to escape from the closed peritrophic sac, which was either excreted or retained intact in the midgut. After five days, only 31% of the flies were infected; attached parasites colonized the pylorus-ileum and/or colon regions of the hindgut. Anterior migration into the cardia region of the midgut occurred in less than 1% of infected flies; no parasites colonized the foregut. In the bloodmeal and residual bloodmeal, five morphologic forms developed from ingested amastigotes: stumpy, spatulate, elongate, short nectomonad promastigotes, and paramastigotes. Abnormal retention of amastigotes in macrophages and delayed development of promastigote stages was observed. The primary form attached in the hindgut was a pear-shaped haptomonad promastigote. Differentiation of L. panamensis in Ph. papatasi appeared to be similar to that described in natural hosts, except that metacyclic infective forms were not observed, and some forms developed in unusual locations. Phlebotomus papatasi was a partly refractory biological host for L. panamensis. The peritrophic membrane adversely affected the infection rate; rare anterior migration and a lack of metacyclic promastigotes may preclude transmission by bite.
Asunto(s)
Leishmania braziliensis/crecimiento & desarrollo , Phlebotomus/parasitología , Animales , Interacciones Huésped-Parásitos , Insectos Vectores/parasitología , Intestinos/parasitología , Intestinos/ultraestructura , Leishmania braziliensis/fisiología , Leishmania braziliensis/ultraestructura , Phlebotomus/ultraestructura , Píloro/parasitología , Píloro/ultraestructura , Especificidad de la EspecieRESUMEN
The effect of ivermectin (0.1 microgram/ml) on blood digestion, ovarian development, and ovipositional attributes of Aedes aegypti was studied using standard morphological and histological techniques. Uncoordinated movements and paralysis were observed in most ivermectin-treated females within 1 h after ingestion of blood containing the chemical. Eight days after the blood meal, 23.5% of the treated females had died, whereas no mortality occurred in controls. Formation of the peritrophic membrane and digestion of the blood meal were delayed in the surviving treated mosquitoes. The most striking effect of ivermectin on Ae. aegypti at this dosage was on ovarian development. Changes observed among ivermectin-treated mosquitoes included: blood digestion without development of ovarian follicles; degeneration of primary follicles and formation of ovarian dilatations within 24 h after ingestion of the chemical; significant reduction in the rate of vitellogenesis and follicle development; decreased egg production; reduced egg hatching; abnormal egg size and shape; and increased percentages of unhatched embryonated and sterile eggs. Although the precise action of ivermectin on Ae. aegypti is unknown, our studies indicate that the chemical directly or indirectly affects at least three major organ systems (nervous, digestive, and reproductive).
Asunto(s)
Aedes , Ivermectina , Animales , Digestión/efectos de los fármacos , Femenino , Ovario/efectos de los fármacos , Oviposición/efectos de los fármacosRESUMEN
The development of Leishmania chagasi, etiologic agent of American visceral leishmaniasis, was studied by light and electron microscopy in the gut of the sand fly, Lutzomyia longipalpis, a natural vector. New aspects of suprapylarian Leishmania behavior were elucidated. In the sand fly midgut, amastigotes transformed into promastigotes (division promastigote I) during a first division sequence within the bloodmeal. Secondary division of these promastigotes resulted in a second form (division promastigote II), and these subsequently elongated into nectomonad promastigotes. Nectomonads existed in long and short populations which divided in the bloodmeal and throughout the midgut lumen after escape from the peritrophic membrane. Nectomonads adhered to the midgut cells in a highly organized manner, with their flagella embedded deep into microvilli and cytoplasm. Migration of parasites from the posterior midgut into the cardia/stomodeal valve region at 36 hr was associated with breakdown of the peritrophic membrane anteriorly. Posterior breakdown at 48 hr resulted in a peritrophic tube open at both ends containing some parasites within the digesting bloodmeal for up to 6 days postinfection. At the stomodeal valve, a myriad of slender and rounded promastigotes attached to the intima by flagellar hemidesmosomes; these may represent a transformation sequence from slender nectomonads to pear-shaped haptomonads. Pear-shaped forms appear to be precursors of paramastigotes, which also attached to the valve intima. Both rounded haptomonads and paramastigotes were found in the esophagus, dividing in a complex sequence initiated by posterior cleavage of the cytoplasm producing unique heart-shaped forms. Dividing paramastigotes also colonized the pharynx up to the cibarial valve. The ultrastructure of paramastigotes suggested that they may be infective forms, capable of some motility in the foregut. Free-swimming "infective" promastigotes were observed throughout the midgut and foregut, were attached in the pharynx (armature region), and were associated with the labrum-epipharynx of the proboscis in 3.6% of flies (16 days). The fine structure of hemidesmosomes in the foregut showed regional specializations, including the presence of plasmalemmar bridges in the gap space.
Asunto(s)
Insectos Vectores/parasitología , Leishmania/ultraestructura , Psychodidae/parasitología , Animales , Femenino , Leishmania/crecimiento & desarrollo , Leishmania/fisiología , Microscopía ElectrónicaRESUMEN
The development of Leishmania (Viannia) panamensis in a natural sand fly host, Lutzomyia gomezi, was studied by light and transmission electron microscopy. New aspects of peripylarian parasite behavior and morphology in the sand fly gut, early bloodmeal stages, and ultrastructural development in the anterior gut were documented. Eight distinct morphological forms were observed in the life cycle of the parasite within the insect. In the bloodmeal, amastigotes (1) transformed into stumpy promastigotes (2) which rapidly multiplied, resulting in spatulate-shaped nectomonad promastigotes (3) and elongate nectomonad promastigotes (4). These latter forms migrated primarily into the hindgut, where both were observed attached (=haptomonad phase) to the cuticular intima by hemidesmosomes within extremely shortened flagella. Spatulate haptomonad promastigotes predominated, colonizing the entire length of the hindgut, with the greatest density at 2 disjunct sites: the pylorus/ileum and the anterior rectum/rectal sac. Paramastigotes and dividing flagellates were rare. Some parasites migrated directly to the cardia/stomodeal valve region without a hindgut phase; however, major movement anteriorly was from the hindgut beginning at 6 days postinfection. In the cardia lumen, dividing short Type A promastigotes (5) predominated, intermixed with short Type B promastigotes with longer flagella (6). Paramastigotes (7) were free-swimming in the lumen as well as attached to the stomodeal valve. The primary colonizers of the valve were pear-shaped haptomonad promastigotes (8), with flagella of variable lengths and multi-segmented hemidesmosomal attachment points to the intima. Promastigotes and paramastigotes colonized the esophagus-pharynx region and attached to the foregut lining by flagellar hemidesmosomes. Both forms may represent infective stages of L. (V.) panamensis; however, no parasites were detected in the cibarium or proboscis. L. (V.) panamensis appeared well-adapted to the gut of Lu. gomezi, multiplying extensively at 2 sites, changing morphological form, and adhering to host surfaces by variously modified flagellar hemidesmosomes.
Asunto(s)
Insectos Vectores/parasitología , Leishmania braziliensis/crecimiento & desarrollo , Leishmania/crecimiento & desarrollo , Psychodidae/parasitología , Animales , Cardias/parasitología , Desmosomas/ultraestructura , Sistema Digestivo/parasitología , Flagelos/ultraestructura , Interacciones Huésped-Parásitos , Leishmania braziliensis/ultraestructura , Microscopía ElectrónicaRESUMEN
Five isolations of the Alagoas serotype of vesicular stomatitis virus (Rhabdoviridae: Vesiculovirus) were made from naturally infected phlebotomine sand flies (Lutzomyia spp.) collected in Colombia. These are the first isolations of Alagoas virus from an arthropod. Replication of the virus occurred in laboratory-reared sand flies (Lutzomyia longipalpis) after inoculation. Bite and transovarial transmission of the virus was also demonstrated in experimentally infected sand flies. Alagoas virus neutralizing antibodies were found in sera of humans and animals living near the insect collection site; antibody rates among human residents of two nearby towns were 63% and 83%, respectively. Results of comparative serologic studies demonstrated that Alagoas virus is closely related antigenically to Indiana, Cocal, and Maraba viruses and that these four agents form a complex within the vesicular stomatitis virus serogroup. The antigenic similarity among these four viruses makes their differentiation difficult; it also raises doubts about the accuracy of current laboratory methods used for identifying isolates in this serogroup. A discussion follows on the significance of human antibodies to these agents and on the role of sand flies in their ecology.
Asunto(s)
Psychodidae/microbiología , Virus de la Estomatitis Vesicular Indiana/aislamiento & purificación , Virosis/epidemiología , Animales , Anticuerpos Antivirales/análisis , Antígenos Virales/inmunología , Colombia , Femenino , Humanos , Masculino , Pruebas de Neutralización , Serotipificación , Estomatitis/epidemiología , Estomatitis/veterinaria , Virus de la Estomatitis Vesicular Indiana/clasificación , Virus de la Estomatitis Vesicular Indiana/crecimiento & desarrollo , Virus de la Estomatitis Vesicular Indiana/inmunología , Virosis/veterinariaRESUMEN
The life cycle of Leishmania mexicana mexicana in the gut of the sand fly, Lutzomyia abonnenci, was studied by light and electron microscopy. Development was suprapylarian with initial establishment of parasites in the bloodmeal (posterior midgut), and anterior migration of parasites to the cardia/stomodeal valve region beginning at 2.5 days post-infection. Flagellates were first observed in the esophagus at 3.5 days, in the posterior armature region of the pharynx at 5 days, and in the anterior pharynx at 7 days; but they were not detected in the cibarium or proboscis. Infection of the pylorus region of the hindgut and of the Malpighian tubules was also commonly observed. Three different morphological forms of L. m. mexicana developed in the gut: nectomonad promastigotes, short promastigotes, and paramastigotes. Nectomonads occurred primarily in the abdominal midgut after bloodmeal digestion, where they were oriented in longitudinal masses in the lumen, or interdigitated with epithelial microvilli via the flagellum. Short promastigotes found in the cardia/stomodeal valve region are described for the first time. These forms were smaller than nectomonads, showed an amplification of the kinetoplast, apposition of kinetoplast and nucleus, and were embedded in a gel-like matrix. To maintain position in the cardia, parasites commonly inserted the flagellum deep into microvilli or cytoplasm of the epithelium; adherence to the cuticular intima of the stomodeal valve was by flagellar modification and formation of hemidesmosome plaques. Paramastigotes occurred in the esophagus, were sometimes degenerated in appearance, and were attached via flagellar hemidesmosomes. Paramastigotes observed in the lumen of the pharynx were commonly degenerated and were not attached to the intima. L. m. mexicana was able to colonize the various gut habitats of Lu. abonnenci by a number of adaptations; this sand fly appears to be a suitable biological host for the parasite.
Asunto(s)
Leishmania mexicana/fisiología , Psychodidae/parasitología , Animales , Sistema Digestivo/ultraestructura , Femenino , Interacciones Huésped-Parásitos , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica , Psychodidae/ultraestructuraRESUMEN
Cutaneous sclerodermalike changes are a well-documented manifestation of chronic cutaneous graft-vs-host reaction. We describe a patient with chronic cutaneous graft-vs-host reaction who developed vesicles and bullae on sclerodermoid skin 18 months after bone marrow transplantation. The vesicles and bullae were subepidermal in location by light microscopy and were associated with dilated lymphatics and a sparse perivascular mononuclear cell infiltrate. No deposition of immunoreactants was seen by immunofluorescent microscopy. Electron microscopy confirmed the presence of a subepidermal blister beneath an intact basement membrane zone and surrounded by marked dermal edema. We postulate that localized lymphedema may play a role in the development of these vesicles and bullae.
Asunto(s)
Enfermedad Injerto contra Huésped/complicaciones , Esclerodermia Sistémica/etiología , Enfermedades Cutáneas Vesiculoampollosas/etiología , Adolescente , Trasplante de Médula Ósea , Enfermedad Crónica , Femenino , Humanos , Esclerodermia Sistémica/patología , Piel/patología , Enfermedades Cutáneas Vesiculoampollosas/patologíaRESUMEN
Chronic cutaneous graft-versus-host reaction following bone marrow transplantation has been difficult to manage in some patients because of poor response to immunosuppressive agents or because of toxicity to these drugs. We recently used methoxsalen and ultraviolet A therapy and found it to be successful in controlling chronic cutaneous lichenoid graft-versus-host reaction in a bone marrow transplant patient. Although there was an initial flare of an eruption resembling acute cutaneous graft-versus-host reaction, both this acute eruption and the lichenoid lesions subsided and cleared with continued treatment. The skin lesions recurred when treatment was discontinued but responded promptly when it was initiated for the second time. Other than mild phototoxicity, there were no other significant side effects.
Asunto(s)
Reacción Injerto-Huésped/efectos de los fármacos , Liquen Plano/tratamiento farmacológico , Terapia PUVA , Fotoquimioterapia , Adolescente , Biopsia , Enfermedad Crónica , Femenino , Humanos , Liquen Plano/patología , Melanocitos/patologíaRESUMEN
Malignant mesotheliomas of the pleura generally cause death by progressive encasement of the lung, but characteristically do not form large tumor masses or deeply invade the lung. Symptoms of pericardial involvement may be present in about 9% of patients at presentation, but at autopsy up to 67% are alleged to extension of the tumor to the pericardium. Infiltration of the myocardium occurs less frequently, but the exact frequency is unknown, and the extent of invasion and clinical effects are poorly documented. The authors report a case of a malignant mesothelioma of the pleura, which extended to involve the pericardium, necessitating pericardiectomy. Subsequently, the patient died as a result of tumor growth through the right atrial wall forming a large intraatrial mass that occluded the tricuspid orifice.
Asunto(s)
Neoplasias Cardíacas/patología , Mesotelioma/patología , Neoplasias Pleurales/patología , Estenosis de la Válvula Tricúspide/etiología , Anciano , Atrios Cardíacos , Humanos , Neoplasias Pulmonares/patología , Masculino , Miocardio , Invasividad Neoplásica , Cuidados Paliativos , Pericardio , TóraxRESUMEN
A house-to-house survey of dogs in a 26-km2 area of Pleasants Valley (Northern California) was done to determine the prevalence of the filariids Dirofilaria immitis Leidy and Dipetalonema reconditum Grassi in the canine population. Blood samples were taken from the cephalic vein of 97 dogs (greater than or equal to 5 months of age) and were tested for the presence of microfilariae, using a modification of the Knott technique. Two dogs with autochthonous D immitis (2.1% of the dogs examined) were discovered. These dogs were outdoor-housed working or sporting dogs, 5 to 8 years old, 1 female and 1 male. Five male dogs, greater than 2 years of age (5.1%), were positive for Dip reconditum microfilariae. Mixed infections were not detected.