Gastric precancerous lesions:occurrence, development factors, and treatment.

Patients with gastric precancerous lesions (GPL) have a higher risk of gastric cancer (GC). However, the transformation of GPL into GC is an ongoing process that takes several years. At present, several factors including H.Pylori (Hp), flora imbalance, inflammatory factors, genetic variations, Claudin-4, gastric stem cells, solute carrier family member 26 (SLC26A9), bile reflux, exosomes, and miR-30a plays a considerable role in the transformation of GPL into GC. Moreover, timely intervention in the event of GPL can reduce the risk of GC. In clinical practice, GPL is mainly treated with endoscopy, acid suppression therapy, Hp eradication, a cyclooxygenase-2 inhibitor, aspirin, and diet. Currently, the use of traditional Chinese medicine (TCM) or combination with western medication to remove Hp and the use of TCM to treat GPL are common in Asia, particularly China, and have also demonstrated excellent clinical efficacy. This review thoroughly discussed the combining of TCM and Western therapy for the treatment of precancerous lesions as conditions allow. Consequently, this review also focuses on the causes of the development and progression of GPL, as well as its current treatment. This may help us understand GPL and related treatment.

Lever arm on bracket vs. lever arm on archwire: A 3D finite element method study of mechanics of miniscrew-supported lingual en-masse retraction of maxillary anterior teeth.

OBJECTIVE: To compare the deformation of the main archwire and 3D movements of maxillary anterior teeth during miniscrew-supported en-masse retraction with the lever arm on the archwire and on the brackets in lingual orthodontic treatment in finite element analysis (FEM) simulation. MATERIAL AND METHODS: A 3D dental-alveolar model with bonded 0.018×0.025-inch slot lingual brackets and a 0.017×0.025-inch dimension stainless-steel archwire was created. Four FEM models were created based on a 3D dental-alveolar model: in Models A and C, the lever arms were attached to the lingual bracket, while in Models B and D, the lever arms were attached to the archwire. Meanwhile, in Models A and B, the miniscrews were placed in between the molars, while in Models C and D, the miniscrews were positioned on the palatal roof. After a 1.5N retraction force was applied from the miniscrew to the end of the lever arm, the initial movements in the sagittal, transversal, and vertical planes were recorded and analysed for maxillary anterior teeth. RESULTS: In Models B and D, smaller deformation of the main archwire and less prominent bowing effect were noticed in both sagittal and vertical directions compared to their counter groups. In Models C and D, the central incisors showed less torque loss in the sagittal direction and more canine intrusion vertically. CONCLUSIONS: For the same lever arm-miniscrew retraction configuration, the lever arm on the bracket showed less deformation of the main archwire and more body movement of the teeth than the lever arm on the archwire group. With the same level arm height, the transverse and vertical bowing effect is reduced when the lever arm was placed distal to the central incisor and the miniscrews placed next to the palatal suture.

Complete genome sequence analysis and biological characteristics of Newcastle disease viruses from different hosts in China.

Newcastle disease (ND) is one of the most serious diseases affecting poultry worldwide. In 2022, we studied two strains of Newcastle disease virus (NDV) from pigeons and magpies identified by PCR and propagated in SPF chicken embryos. The whole genome of the virus was then expanded and its biological characteristics were studied. The results showed that NDV was isolated from pigeons and magpies. Virus present in the allantoic fluid could agglutinate red blood cells and could not be neutralized by serum positive for avian influenza. Sequencing showed that the gene length of the two isolates was 15,191 bp, had high homology and was located in the same branch of the phylogenetic tree, both belonging to genotype VI.1.1. The sequence of 112-117 amino acids in the F gene sequence was 112R-R-Q-K-R-F117, which constituted virulent strain characteristics. The HN gene contained 577 amino acids, which is also consistent with the characteristics of a virulent strain. The results from the study of biological characteristics revealed that the virulence of SX/TY/Pi01/22 was slightly stronger. There were only four different bases in the complete sequence of the two strains. Comprehensive analysis revealed that the G at 11,847 site of the SX/TY/Ma01/22 strain may change to T, leading to translation of amino acids from R to S, thereby weakening viral virulence. Therefore, NDV was transmitted from pigeons to magpies, indicating that the pathogen could be transmitted between poultry and wild birds.

Quantification of orthodontic loads on teeth in the correction of canine overeruption using different archwire designs.

INTRODUCTION: This study quantifies the effects of material, size of the continuous archwires, and level of overeruption on the loads on teeth in the correction of overerupted canines. METHODS: An orthodontic force test (OFT) was used to measure the 3-dimensional loads delivered by the archwires to the brackets attached to the maxillary right incisors, canine, and premolars. Dentoforms simulating canine overeruptions at the 0.5 mm and 1 mm levels were made from computerized tomography scans. Archwires with 2 types of material (stainless steel [SS] and nickel-titanium [NiTi]) and 2 sizes (0.014-in and 0.016-in) were tested, respectively, on the 0.022 × 0.028-in brackets through elastomeric ligatures. RESULTS: The forces were dominantly intrusive on the canines and extrusive on the first premolars and lateral incisors. The magnitudes of the extrusive forces were about 74% and 52% that of intrusive force on the canines, which range from -0.48 ± 0.01 N to -5.70 ± 0.14 N depending on the wire material, size, and severity of overeruption (P <0.01). The canine intrusive forces created by SS wires were about 3 times higher than that of NiTi wires with the same sizes, 0.016-in archwires were about twice higher than that of 0.014-in with the same materials, and 1 mm overeruption level doubled with respect to 0.5 mm. Significant second-order moment as coupled with the intrusive or extrusive forces. CONCLUSIONS: The intrusive and extrusive forces on teeth in the correction of canine overeruption can be quantified by the in vitro orthodontic force test, and the effects of the 3 factors significantly affect the loads on the teeth.

Biomechanical analysis of maxillary anterior teeth movements during different retracting methods with a lever arm miniscrew system in double-slot lingual brackets: A finite element method study.

OBJECTIVE: The objective of the study was to find the most effective retracting method for the six maxillary anterior teeth with double-slot lingual brackets using a lever arm and miniscrew system. METHODS: Six finite element models were constructed: (A) a ribbon-wise archwire with a lever arm; (B) a ribbon-wise archwire with a lever arm and a sectional edgewise archwire; (C) a ribbon-wise archwire and a sectional edgewise archwire with a lever arm; (D) an edgewise archwire with a lever arm; (E) an edgewise archwire with a lever arm and a sectional ribbon-wise archwire; and (F) an edgewise archwire and a sectional ribbon-wise archwire with a lever arm. Initial displacements of maxillary teeth driven by a 1.5 N retraction force to the lever arm were analysed. RESULTS: In models B, C, E and F, anterior torque loss and inter-canine distance increases were significantly lower than that of the models without auxiliary archwires. In models C and F, the main archwire was less likely deformed. In models A, B and C, the displacement of canines in sagittal and vertical directions were less than in models D, E and F. CONCLUSIONS: The combination of edgewise and ribbon-wise archwires in double-slot lingual brackets effectively preserves the anterior arch shape. An edgewise archwire with lever arms on the auxiliary sectional ribbon-wise archwire is recommended for better performance in anterior teeth retraction.

The effects of different types of periodontal ligament material models on stresses computed using finite element models.

INTRODUCTION: Finite element (FE) method has been used to calculate stress in the periodontal ligament (PDL), which is crucial in orthodontic tooth movement. The stress depends on the PDL material property, which varies significantly in previous studies. This study aimed to determine the effects of different PDL properties on stress in PDL using FE analysis. METHODS: A 3-dimensional FE model was created consisting of a maxillary canine, its surrounding PDL, and alveolar bone obtained from cone-beam computed tomography scans. One Newton of intrusion force was applied vertically to the crown. Then, the hydrostatic stress and the von Mises stress in the PDL were computed using different PDL material properties, including linear elastic, viscoelastic, hyperelastic, and fiber matrix. Young's modulus (E), used previously from 0.01 to 1000 MPa, and 3 Poisson's ratios, 0.28, 0.45, and 0.49, were simulated for the linear elastic model. RESULTS: The FE analyses showed consistent patterns of stress distribution. The high stresses are mostly concentrated at the apical area, except for the linear elastic models with high E (E >15 MPa). However, the magnitude varied significantly from -14.77 to -127.58 kPa among the analyzed patients. The E-stress relationship was not linear. The Poisson's ratio did not affect the stress distribution but significantly influenced the stress value. The hydrostatic stress varied from -14.61 to -95.48 kPa. CONCLUSIONS: Different PDL material properties in the FE modeling of dentition do not alter the stress distributions. However, the magnitudes of the stress significantly differ among the patients with the tested material properties.

Peak loads on teeth from a generic mouthpiece of a vibration device for accelerating tooth movement.

INTRODUCTION: The effect of vibrational force (VF) on accelerating orthodontic tooth movement depends on the ability to control the level of stimulation in terms of its peak load (PL) on the tooth. The objective of this study was to investigate the PL distribution on the teeth when a commercial VF device is used. METHODS: Finite element models of a human dentition from cone-beam computed tomography images of an anonymous subject and a commonly used commercial VF device were created. The device consists of a mouthpiece and a VF source. The maxilla and mandible bites on the mouthpiece with the VF applied to it. Interface elements were used between the teeth and the mouthpiece, allowing relative motion at the interfaces. The finite element model was validated experimentally. Static load and VF with 2 frequencies were used, and the PL distributions were calculated. The effects of mouthpiece materials and orthodontic appliances on the PL distribution were also investigated. RESULTS: The PL distribution of this kind of analyzed device is uneven under either static force or VF. Between the anterior and posterior segments, the anterior segment receives the most stimulations. The mouthpiece material affects the PL distribution. The appliance makes the PL more concentrated on the incisors. The VF frequencies tested have a negligible influence on both PL magnitude and distribution. CONCLUSIONS: The device analyzed delivers different levels of stimulation to the teeth in both maxilla and mandible. Changing the material property of the mouthpiece alters the PL distribution.

[Effects of different alveolar bone finite element models on the biomechanical responses of periodontal ligament].

In the study of oral orthodontics, the dental tissue models play an important role in finite element analysis results. Currently, the commonly used alveolar bone models mainly have two kinds: the uniform and the non-uniform models. The material of the uniform model was defined with the whole alveolar bone, and each mesh element has a uniform mechanical property. While the material of the elements in non-uniform model was differently determined by the Hounsfield unit (HU) value of computed tomography (CT) images where the element was located. To investigate the effects of different alveolar bone models on the biomechanical responses of periodontal ligament (PDL), a clinical patient was chosen as the research object, his mandibular canine, PDL and two kinds of alveolar bone models were constructed, and intrusive force of 1 N and moment of 2 Nmm were exerted on the canine along its root direction, respectively, which were used to analyze the hydrostatic stress and the maximal logarithmic principal strain of PDL under different loads. Research results indicated that the mechanical responses of PDL had been affected by alveolar bone models, no matter the canine translation or rotation. Compared to the uniform model, if the alveolar bone was defined as the non-uniform model, the maximal stress and strain of PDL were decreased by 13.13% and 35.57%, respectively, when the canine translation along its root direction; while the maximal stress and strain of PDL were decreased by 19.55% and 35.64%, respectively, when the canine rotation along its root direction. The uniform alveolar bone model will induce orthodontists to choose a smaller orthodontic force. The non-uniform alveolar bone model can better reflect the differences of bone characteristics in the real alveolar bone, and more conducive to obtain accurate analysis results.

Numerical simulation of optimal range of rotational moment for the mandibular lateral incisor, canine and first premolar based on biomechanical responses of periodontal ligaments: a case study.

OBJECTIVES: The objective of this study was to investigate the optimal range of rotational moment for the mandibular lateral incisor, canine and first premolar to determine tooth movements during orthodontic treatment using hydrostatic stress and logarithmic strain on the periodontal ligament (PDL) as indicators by numerical simulations. MATERIAL AND METHODS: Teeth, PDL and alveolar bone numerical models were constructed as analytical objects based on computed tomography (CT) images. Teeth were assumed to be rigid bodies, and rotational moments ranging from 1.0 to 4.0 Nmm were exerted on the crowns. PDL was defined as a hyperelastic-viscoelastic material with a uniform thickness of 0.25 mm. The alveolar bone model was constructed using a non-uniform material with varied mechanical properties determined based on Hounsfield unit (HU) values calculated using CT images, and its bottom was fixed completely. The optimal range values of PDL compressive and tensile stress were set as 0.47-12.8 and 18.8-51.2 kPa, respectively, whereas that of PDL logarithmic strain was set as 0.15-0.3%. RESULTS: The rotational tendency of PDL was around the long axis of teeth when loaded. The optimal range values of rotational moment for the mandibular lateral incisor, canine and first premolar were 2.2-2.3, 3.0-3.1 and 2.8-2.9 Nmm, respectively, referring to the biomechanical responses of loaded PDL. Primarily, the optimal range of rotational moment was quadratically dependent on the area of PDL internal surface (i.e. area of PDL internal surface was used to indicate PDL size), as described by the fitting formula. CONCLUSIONS: Biomechanical responses of PDL can be used to estimate the optimal range of rotational moment for teeth. These rotational moments were not consistent for all teeth, as demonstrated by numerical simulations. CLINICAL RELEVANCE: The quantitative relationship between the area of PDL internal surface and the optimal orthodontic moment can help orthodontists to determine a more reasonable moment and further optimise clinical treatment.

Dynamic measurement of orthodontic force using a tooth movement simulation system based on a wax model.

BACKGROUND: Orthodontic force is often statically measured in general, and only the initial force derived from appliances can be assessed. OBJECTIVE: We aimed to investigate a technological method for measuring dynamic force using tooth movement simulation. METHODS: Tooth movement was simulated in a softened wax model. A canine tooth was selected for evaluation and divided into the crown and root. A force transducer was plugged in and fixed between the two parts for measuring force. Forces on this tooth were derived by ordinary nickel-titanium (Ni-Ti) wire, hyperelastic Ni-Ti wire, low-hysteresis (LH) Ti-Ni wire and self-made glass fibre-reinforced shape memory polyurethane (GFRSMPU) wire. These forces were measured after the tooth movement. RESULTS: The canine tooth moved to the desired location, and only a 0.2 mm deviation remained. The changing trends and magnitudes of forces produced by the wires were consistent with the data reported by other studies. The tooth had a higher moving velocity with ordinary Ni-Ti wires in comparison to the other wires. Force attenuation for the GFRSMPU wire was the lowest (40.17%) at the end of the test, indicating that it provided light but continuous force. CONCLUSIONS: Mimicked tooth movements and dynamic force measurements were successfully determined in tooth movement simulation. These findings could help with estimating treatment effects and optimising the treatment plan.

A systematic review of the effectiveness and safety of Chinese herbal medicine formula Gualou Xiebai Banxia (GLXBBX) decoction for the treatment of stable angina pectoris.

BACKGROUND: A growing number of studies suggest that Gualou Xiebai Banxia (GLXBBX) decoction, a well-known Chinese herbal formula, has beneficial effects on eliminating angina pectoris symptoms and improving condition of stable angina pectoris (SAP) patients. However, whether this treatment is effective and safe for SAP or not, evidence supporting the effectiveness and safety of this treatment is still incomplete. Besides, there is lack of systematic review to assess the detailed situation (including risk of bias and methodology) of current related clinical studies. This study aimed to evaluate the effectiveness and safety of GLXBBX in treating SAP. METHODS: The major databases (MEDLINE, Embase, the Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese Scientific Journals Database (VIP) Database, Chinese Biomedical Database (CBM), Chinese Biomedical Literature Service System (SinoMed), and Wanfang Database) were searched from inception to March 2019. Randomized controlled trials (RCTs) of GLXBBX alone or combined with conventional drugs against conventional drugs for SAP were identified. Two assessors reviewed each trial independently. The methodological quality of the eligible studies was evaluated according to the Cochrane Collaboration's tool for assessing risk of bias. Both the data extraction and the literature quality screening evaluation were conducted independently by 2 researchers. RESULT: Totally 17 clinical RCTs were included in this study, involving 1676 patients. Due to the high probability of bias of the included studies, it was inappropriate to undertake a meta-analysis. Thus, we only conducted a systematic review and mainly discussed the methodology and limitation of the included studies. CONCLUSION: Although the current evidence prompted that GLXBBX might benefit SAP patients in improvement of angina pectoris, ECG, and blood lipid on a certain extent, this systematic review revealed no definite conclusion about the application of GLXBBX for SAP due to the poor methodological quality, high risk of bias, and inadequate reporting on clinical data. More rigorous, multicenter, sufficient-sample, and double-blind randomized clinical trials are warranted.

Investigation of effective intrusion and extrusion force for maxillary canine using finite element analysis.

Orthodontic tooth movement is mainly regulated by the biomechanical responses of loaded periodontal ligament (PDL). We investigated the effective intervals of orthodontic force in pure maxillary canine intrusion and extrusion referring to PDL hydrostatic stress and logarithmic strain. Finite element analysis (FEA) models, including a maxillary canine, PDL and alveolar bone, were constructed based on computed tomography (CT) images of a patient. The material properties of alveolar bone were non-uniformly defined using HU values of CT images; PDL was assumed to be a hyperelastic-viscoelastic material. The compressive stress and tensile stress ranging from 0.47 to 12.8 kPa and 18.8 to 51.2 kPa, respectively, were identified as effective for tooth movement; a strain 0.24% was identified as the lower limit of effective strain. The stress/strain distributions within PDL were acquired in canine intrusion and extrusion using FEA; root apex was the main force-bearing area in intrusion-extrusion movements and was more prone to resorption. Owing to the distinction of PDL biomechanical responses to compression and tension, the effective interval of orthodontic force was substantially lower in canine intrusion (80-90 g) than in canine extrusion (230-260 g). A larger magnitude of force remained applicable in canine extrusion. This study revised and complemented orthodontic biomechanical behaviours of tooth movement with intrusive-extrusive force and could further help optimize orthodontic treatment.

Photodynamic therapy versus systemic antibiotic for the treatment of periodontitis in a rat model.

BACKGROUND: To compare the therapeutic effect of photodynamic therapy (PDT) with Toluidine blue O hydrogel versus systemic antibiotic (SA) in treating periodontitis on rats. METHODS: Thirty-two Wistar rats were divided into four groups and treated differently: Negative control (NC) group, normal rats; positive control (PC) group, rats with periodontitis; SA group, rats with periodontitis treated with systemic antibiotic; PDT group, rats with periodontitis treated with PDT. After treatment, gingival sulcus bacterial load was measured by counting the colony forming units per milliliter (CFU mL-1 ). The tooth and periodontal tissues were histologically processed to analyze histological and immunohistochemical profile. Gingival samples were obtained to quantify interleukin-1 beta (IL-1ß) and tumor necrosis factor-alpha (TNF-α) levels. RESULTS: Gingival sulcus bacteria load is significantly lower in PDT group compared with the SA group. The histological analysis showed that some extremely effective repair signs of periodontal tissue were presented in PDT group, such as no periodontal pocket, no bone resorption, few inflammatory cells, massive fibroblasts and collagen fibers. Several effective repair signs of periodontal tissue were also observed in SA group, such as shallow periodontal pocket, small amount of inflammatory cells, substantial fibroblasts and collagen fibers. There were lower cyclooxygenase-2, matrix metalloproteinase -8 (MMP-8) and RANK immunolabeling, higher osteoprotegerin immunolabeling in PDT group compared with SA group. The IL-1ß and TNF-α levels in PDT group were lower than those in NC group, but higher than those in SA group. CONCLUSION: PDT was effective to treat experimental periodontitis and was superior to systemic metronidazole as a treatment for periodontitis.

Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage.

BACKGROUND: Macrophage polarization has been reported to induce podocyte injury, which is a typical characteristic of diabetic nephropathy (DN). Trichosanthes kirilowii is an herb showing renal protective effect as well as immune-regulating effect. Therefore, it was hypothesized that the renal protective effect of Trichosanthes kirilowii was associated with its modulation on macrophage polarization. In the current study, we tested the hypothesis by subjecting DN rats to treatment of Trichosanthes kirilowii lectin (TKL), an active component of Trichosanthes kirilowii. METHOD: DN was induced using streptozocin (STZ) method, and after 3 days, treatments were performed with different doses of TKL for eight weeks. The effect of TKL on the renal function, structure, and inflammation was assessed. To explain the pathway mediating the effect of TKL on renal tissues, the expressions of markers involved in macrophage polarization, podocyte proliferation, and Notch signaling were determined. Moreover, the DN rats were further administrated with Notch signaling inhibitor, Dibenzazepine (DIB), to verify the key role of Notch signaling in the renal protective effect of TKL. RESULTS: STZ induced damages in renal function and structure, which was attenuated by TKL of different doses. Moreover, STZ also increased the production of TNF-α and iNOS while suppressed the production of IL-10 and arginase-1 (Arg-1). The induced inflammation by STZ was inhibited by TKL. The polarization of macrophage into M1 type during the development of DN was blocked by TKL, contributing to the increased proliferation potential of podocytes. Regarding Notch signaling, TKL administration inhibited the activation of the pathway by suppressing the expression of Notch1, NICD1, and Hes1. The administration of DIB had similar effect to that of TKL administration on renal function and structure. CONCLUSIONS: The study for the first time showed that TKL attenuated deterioration in renal structure and function by increasing M2 macrophage proportion via inhibition of Notch signaling.

Jian-Pi-Yi-Shen Formula Regulates Inflammatory Cytokines Production in 5/6 Nephrectomized Rats via Suppression of NF-κB Activation.

Jian-Pi-Yi-Shen formula (JPYSF) is a Chinese herbal decoction used for treating chronic kidney disease (CKD) for over 20 years with good efficiency. However, the mechanism lacks solid evidence. In the present study, we tested the hypothesis that JPYSF may retard CKD progression via inhibition of inflammation in 5/6 nephrectomy (5/6 Nx) rat model. The 5/6 Nx rats were randomly divided into 2 groups: 5/6 Nx group and JPYSF group. Sham-operated rats served as control. JPYSF (2.06 g/kg/d) were administrated by gavage to 5/6 Nx rats daily for 6 weeks. Results showed that JPYSF treatment significantly improved kidney function and pathological injury in 5/6 Nx rats. Multiplex analysis of cytokines revealed that JPYSF reduced proinflammatory cytokines and increased anti-inflammatory cytokine production. Furthermore, JPYSF inhibited the activation of nuclear factor-kappa B (NF-κB) signaling pathway. In conclusion, our data demonstrated that JPYSF remarkably retards development and progression of CKD in a 5/6 Nx rat model, which may be associated with inhibition of inflammation via NF-κB signaling pathway.

Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway.

Trichosanthes kirilowii lectin (TKL) has been reported to exert hypoglycemic effects in alloxan-induced diabetic mice. However, there is no evidence showing that it helps to prevent diabetic nephropathy (DN). We used a high glucose (HG)-induced HK-2 cell model and a streptozocin (STZ)-induced Wistar rat model to investigate the effects of TKL on DN, as well as the mechanisms for those effects. Our results showed that TKL significantly increased the viability of HG-treated HK-2 cells and inhibited cell apoptosis. In vivo experiments demonstrated that TKL attenuated STZ-induced histopathological damage and the inflammatory response in rat kidney tissues. Pre-treatment of HK-2 cells or STZ-treated rats with polyinosinic acid (Poly IC), an inhibitor of lectin-like oxLDL receptor 1 (LOX1), blocked the protective effect of TKL against HG- or STZ-induced damage to kidney tissue, indicating that TKL might exert its effect via LOX1-mediated endocytosis. Additional results suggested that TKL inhibits the phosphorylation of IκB kinase ß (IKKß) and the nuclear factor-κB (NF-κB) inhibitor protein (IκBα), and thereby reduces the nuclear translocation of NF-κB (p65). ChIP assay data indicated that TKL markedly inhibits the binding of p65 to the CASP9 gene in HG-treated HK-2 cells, subsequently suppressing transcription of the CASP9 gene. In the dual-luciferase reporter assay, TKL significantly inhibited luciferase activity in cells co-transfected with p65 and a wild-type capase-9 construct instead of mutated caspase-9 constructs.Taken together, our results show that TKL helps to protect against DN by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway, suggesting TKL as a promising agent for treating DN.

The comprehensive liver transcriptome of two cattle breeds with different intramuscular fat content.

Intramuscular fat (IMF) content is an important determinant factor of meat quality in cattle. There is significant difference in IMF content between Jinnan and Simmental cattle. Here, to identify candidate genes and networks associated with IMF deposition, we deeply explored the transcriptome architecture of liver in these two cattle breeds. We sequenced the liver transcriptome of five Jinnan and three Simmental cattle, yielding about 413.9 million sequencing reads. 124 differentially expressed genes (DEGs) were detected, of which 53 were up-regulated and 71 were down-regulated in Jinnan cattle. 1282 potentially novel genes were also identified. Gene ontology analysis revealed these DEGs (including CYP21A2, PC, ACACB, APOA1, and FADS2) were significantly enriched in lipid biosynthetic process, regulation of cholesterol esterification, reverse cholesterol transport, and regulation of lipoprotein lipase activity. Genes involved in pyruvate metabolism pathway were also significantly overrepresented. Moreover, we identified an interaction network which related to lipid metabolism, which might be contributed to the IMF deposition in cattle. We concluded that the DEGs involved in the regulation of lipid metabolism could play an important role in IMF deposition. Overall, we proposed a new panel of candidate genes and interaction networks that can be associated with IMF deposition and used as biomarkers in cattle breeding.