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1.
Food Chem ; 239: 911-919, 2018 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-28873652

RESUMEN

ß-Cyclodextrin (ß-CD) modified magnetic graphene oxide material (Fe3O4@SiO2/GO/ß-CD), a novel magnetic sorbent for enrichment and purification of plant growth regulators (PGRs) in vegetables prior to gas chromatograph mass spectrometer (GC-MS) detection, was successfully fabricated. The novel magnetic sorbent geometrically fitted to selectively recognize and enrich PGR analytes especially containing aromatic structure. The selectivity experiment demonstrated the higher selectivity of Fe3O4@SiO2/GO/ß-CD toward 5 PGR compounds than that of Fe3O4@SiO2/GO. Several parameters affecting extraction efficiency including the volume of extraction solvent, amount of adsorbent, extraction time, type and the volume of desorption solvent were optimized. Under the optimized condition, good linear relationships were obtained in the range of 1-100µg/kg with determination coefficients (R2) from 0.9983 to 0.9996. The limits of detection (LODs) of 5 PGRs were from 0.04 to 0.28µg/kg. The proposed method had good potential for the analysis of trace level of 5 PGRs in real vegetable samples.


Asunto(s)
beta-Ciclodextrinas/química , Grafito , Óxidos , Reguladores del Crecimiento de las Plantas , Dióxido de Silicio , Extracción en Fase Sólida
2.
J Endourol ; 31(1): 66-71, 2017 01.
Artículo en Inglés | MEDLINE | ID: mdl-27762624

RESUMEN

PURPOSE: Saving warm ischemia time (WIT) in laparoscopic partial nephrectomy (LPN) is important. We have designed a new device, a remotely activated bulldog clamp, to facilitate LPN. In present study, the effectiveness, convenience, and safety of the new design device were compared with that of standard bulldog vascular clamp in a pig model. MATERIALS AND METHODS: Fifteen cases of LPN were bilaterally operated on upper or lower pole of kidneys in five pigs. As the test group, eight cases involved the remotely activated bulldog clamp, whereas the remaining seven cases, as the control group, involved the common standard vascular bulldog clamp. The mean operative time, blood lost, and mean WIT were compared between the two groups. RESULTS: The mean operative time and blood lost were not significantly different between the groups (p < 0.05). Continuous artery clamping was used in the two groups. With on-demand clamping, the mean WIT in the test group was 19.0 ± 3.42 minutes, which was significantly less compared with the control group (31.54 ± 5.23 minutes, p < 0.05). CONCLUSIONS: The newly designed device can remotely control the renal artery, which made clamping and unclamping much easier. It is effective and safe in LPN.


Asunto(s)
Neoplasias Renales/cirugía , Laparoscopía/instrumentación , Nefrectomía/instrumentación , Instrumentos Quirúrgicos , Animales , Constricción , Modelos Animales de Enfermedad , Diseño de Equipo , Riñón/cirugía , Tempo Operativo , Arteria Renal/cirugía , Sus scrofa , Porcinos , Isquemia Tibia
3.
Am J Transl Res ; 8(2): 1091-9, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27158395

RESUMEN

Maternally Expressed Gene 3 (Meg3) encodes a long non-coding RNA that has been recently shown to regulate tumorigenesis through its interaction with microRNA (miR). We have recently reported that miR-1297 might play a role in the regulation of PTEN/PI3k/Akt signaling pathway in testicular germ cell tumor (TGCT). However, a crosstalk between Meg3 and miR-1297 in TGCT has not been appreciated. Here, we analyzed the levels of Meg3, miR-1297 and PTEN in TGCT specimens, compared to paired adjacent non-tumor tissue (NT), and found that Meg3 levels were significantly decreased and miR-1297 levels were unchanged in TGCT. PTEN protein but not mRNA levels significantly decreased in TGCT. Bioinformatics analyses showed that miR-1297 bound to 3'-UTR of PTEN mRNA, while miR-1297 also bound to Meg3. Luciferase report assay showed that Meg3 overexpression abolished the effects of miR-1297 on 3'-UTR of PTEN mRNA, possibly through competitive binding, which was supported by double fluorescent in situ hybridization showing co-localization of intracellular Meg3 and miR-1297 signals in TGCT cells. Moreover, Meg3 overexpression abolished the inhibitory effects of miR-1297 on PTEN, resulting in deactivation of Akt and decreases in cell growth. Together, these data demonstrate a previous unappreciated pathway in which Crosstalk between Meg3 and miR-1297 regulates growth of TFCT through PTEN/PI3K/AKT signaling. Re-expression of Meg3 may be an attractive strategy for TGCT therapy.

4.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(1): 116-20, 2016 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-26885920

RESUMEN

OBJECTIVE: To investigate the immediate bond strength and surface structure of resin and the tooth enamel which treated by cold plasma. METHODS: In the study, 40 bovine incisors were divided into two equal parts. In this sense, all enamel adhesive samples were prepared and then randomly divided into 4 groups (n =20). group 1: acid + single bond 2+resin composite (control group); group 2:beyond bleaching+ acid+single bond 2+resin composite; group 3: treated by cold plasma for 5 minutes+ acid+single bond 2+resin composite; group 4: treated by cold plasma for 5 minutes+single bond 2+resin composite. Single bond 2 bonding system and Filtek Z250 resin were used in this experiment. The shear bond strength was tested by universal testing machine. The surface of the enamel in different processes was observed by scanning electron microscope (SEM). Statistical analyses by the single factor analysis of variance and multiple pairwise comparisons were performed with SPSS 17.0 . RESULTS: The shear bond strength of group 4 (8.60 MPa) was significantly lower than that of the other three groups (P<0.05). The shear bond strength of group 2 (17.89 MPa) was higher than that of group 4, but lower than group 1 and group 3 (P<0.05).There was no significant difference between group 1 (34.82 MPa) and group 3 (34.69 MPa). Scanning electron microscope indicated that the enamel treated by cold plasma had slight molten form, which was different from etched enamel surface.The fractured surface of group 3 was mix fracture, which was similar to the control group (group 1). CONCLUSION: Compared with the conventional clinic bleaching, immediate bond strength of resin-enamel that treated by cold plasma has not been affected.


Asunto(s)
Recubrimiento Dental Adhesivo , Esmalte Dental , Gases em Plasma , Blanqueamiento de Dientes/métodos , Animales , Bisfenol A Glicidil Metacrilato , Bovinos , Resinas Compuestas , Análisis del Estrés Dental , Cementos de Resina
5.
Asian J Androl ; 17(2): 329-31, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25578936

RESUMEN

Recurrent ischemic priapism is a problem in clinical treatment. Most of the cases require more invasive surgery to shunt the blood stasis. We introduce a modified technique in treating recurrent ischemic priapism. The technique described is applied to acute ischaemic priapic episodes in patients with a history of stuttering priapism. It was carried out by a Winter's shunt combined with a continuous cavernosal irrigation system. Priapism was effectively resolved on the patients without recurrence. The four patients who received this treatment recovered most sexual function after 6 months follow-up.


Asunto(s)
Procedimientos Quirúrgicos Mínimamente Invasivos/métodos , Priapismo/terapia , Irrigación Terapéutica/métodos , Procedimientos Quirúrgicos Urológicos Masculinos/métodos , Adulto , Drenaje/métodos , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Recurrencia , Resultado del Tratamiento
6.
Biomed Environ Sci ; 27(9): 707-17, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25256860

RESUMEN

OBJECTIVE: To study the risk environmental and psycho-social factors associated to prostate cancer (PCa) in Chinese population. METHODS: 250 PCa patients and 500 controls were enrolled in this case-control study. Information was collected and logistic regression analysis was used to estimate the odds ratios (OR) and 95% confidence intervals (95% CI) for relationship between lifestyle, eating habits and psycho-social factors with PCa risk. RESULTS: Green vegetables and green tea were associated with a decreased risk of PCa (OR=0.39, 95% CI: 0.28-0.53; OR=0.59, 95% CI: 0.40-0.87, respectively). Family history of PCa (OR=7.16, 95% CI: 2.01-25.49), history of prostate diseases (OR=2.28, 95% CI: 1.53-3.41), alcohol consumption (OR=1.97, 95% CI: 1.33-2.90), red meat consumption (OR=1.74, 95% CI: 1.20-2.52), barbecued (OR=2.29, 95% CI: 1.11-4.73) or fried (OR=2.35, 95% CI: 1.24-4.43) foods were related with increased PCa risk. Negative psycho-social factors including occupational setbacks (OR=1.61, 95% CI: 1.00-2.59), marital separation (OR=1.94, 95% CI: 1.29-2.91), self-contained suffering (OR=2.37, 95% CI: 1.58-3.55), and high sensitivity to the personal comments (OR=1.73, 95% CI: 1.18-2.54) were related to PCa. CONCLUSION: Regular consumption of green vegetables and green tea may suggest protective effects on PCa. Alcohol consumption, red meat consumption and barbecued or fried foods were associated with PCa. Negative psycho-social factors may also play a role in the incidence of PCa in Chinese population.


Asunto(s)
Neoplasias de la Próstata/epidemiología , Anciano , Anciano de 80 o más Años , Animales , Estudios de Casos y Controles , China/epidemiología , Alimentos/estadística & datos numéricos , Humanos , Estilo de Vida , Masculino , Persona de Mediana Edad , Neoplasias de la Próstata/etiología , Neoplasias de la Próstata/psicología , Estrés Psicológico/complicaciones
7.
Asian Pac J Cancer Prev ; 15(15): 6243-6, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25124605

RESUMEN

To investigate the role of miR-1297 and the tumor suppressor gene PTEN in cell proliferation of testicular germ cell tumors (TGCT). MTT assays were used to test the effect of miR-1297 on proliferation of the NCCIT testicular germ cell tumor cell line. In NCCIT cells, the expression of PTEN was assessed by Western blotting further. In order to confirm target association between miR-1297 and 3'-UTR of PTEN, a luciferase reporter activity assay was employed. Moreover, roles of PTEN in proliferation of NCCIT cells were evaluated by transfection of PTEN siRNA. Proliferation of NCCIT cells was promoted by miR-1297 in a concentration-dependent manner. In addition, miR-1297 could bind to the 3'-UTR of PTEN based on luciferase reporter activity assay, and reduced expression of PTEN at protein level was found. Proliferation of NCCIT cells was significantly enhanced after knockdown of PTEN by siRNA. miR-1297 as a potential oncogene could induce cell proliferation by targeting PTEN in NCCIT cells.


Asunto(s)
Proliferación Celular , Regulación Neoplásica de la Expresión Génica , MicroARNs/genética , Neoplasias de Células Germinales y Embrionarias/genética , Neoplasias de Células Germinales y Embrionarias/patología , Fosfohidrolasa PTEN/metabolismo , Neoplasias Testiculares/genética , Neoplasias Testiculares/patología , Regiones no Traducidas 3'/genética , Western Blotting , Humanos , Luciferasas/metabolismo , Neoplasias de Células Germinales y Embrionarias/metabolismo , Fosfohidrolasa PTEN/antagonistas & inhibidores , Fosfohidrolasa PTEN/genética , ARN Interferente Pequeño/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Neoplasias Testiculares/metabolismo , Células Tumorales Cultivadas
8.
J Sep Sci ; 37(14): 1773-80, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24777951

RESUMEN

In this study, a screening and confirmation method for the determination of l-hydroxyproline (Hyp) as a target compound in milk and dairy products using high-performance liquid chromatography with tandem mass spectrometry was developed. The samples were lyophilized after acidic hydrolysis, followed by cleanup with graphitized carbon black to remove pigments. Hyp was separated by a hydrophilic interaction chromatographic column and analyzed by high-performance liquid chromatography with tandem mass spectrometry working with multiple reaction monitoring mode using an electrospray ionization interface in a positive-ion mode. Average recoveries in spiked milk and dairy products ranged from 68.0 to 101.1% with relative standard deviations between 2.0 and 11.7% (n = 7). A reagent-matched standard calibration curve was used for quantification of Hyp, with linear correlation coefficient (R(2)) > 0.99 in the concentration range of 0.1-100 µg/mL. The LOQs were from 0.25 to 5 mg/kg, which were usually sufficient to verify the Hyp in samples. The confirmation concentration of Hyp ranged from 10 to 50 mg/kg.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Productos Lácteos/análisis , Hidroxiprolina/análisis , Leche/química , Espectrometría de Masas en Tándem/métodos , Animales , Bovinos , Interacciones Hidrofóbicas e Hidrofílicas
9.
Zhonghua Nan Ke Xue ; 20(1): 14-8, 2014 Jan.
Artículo en Chino | MEDLINE | ID: mdl-24527531

RESUMEN

OBJECTIVE: To evaluate the safety and efficacy of tadalafil on demand and on time in men with erectile dysfunction. METHODS: We conducted a multi-centered randomized controlled study on 120 ED males, who were assigned to take tadalafil at 10 mg/ 20 mg on demand before sexual activity and at the same dose on time twice a week for 8 weeks. Before and at 4 and 8 weeks after treatment, and 1 month after withdrawal, we obtained the scores on IIEF-5, ED Inventory of Treatment Satisfaction (EDITS) and the short form of Psychological and Interpersonal Relationship Scales (SF-PAIRS) , and compared the safety and efficacy of medication between the two groups of patients. RESULTS: Totally, 110 patients accomplished the trial, 56 in the on-time and 54 in the on-demand group. At 4 and 8 weeks of medication and 1 month after withdrawal, the IIEF-5 scores were improved in both the on-time and on-demand groups, even more significantly in the former than in the latter at 8 weeks of treatment (21.6 +/- 2.9 vs 18.5 +/- 1.7) and 1 month after withdrawal (20.9 +/- 2.1 vs 17.9 +/- 2.3) (P < 0.05). The EDITS scores were significantly higher in the on-time than in the on-demand group at 8 weeks of treatment (31.7 +/- 6.9 vs 28.6 +/- 5.8) and 1 month after withdrawal (30.6 +/- 4.7 vs 27.9 +/- 6.5) (P < 0.05). The scores on the sexual self-confidence, spontaneity and time-concern domains of SF-PAIRS were remarkably improved after medication as compared with the baseline (P < 0.05), even more significantly in the on-time than in the on-demand group at 1 month after withdrawal. Both dosing schedules were well tolerated and no significant differences were observed in safety between the two groups. CONCLUSION: On-time dosing of tadalafil is efficacious and well tolerated in the treatment of ED, and has an even better effect than on-demand dosing at 8 weeks of medication and 1 month after withdrawal.


Asunto(s)
Carbolinas/administración & dosificación , Disfunción Eréctil/tratamiento farmacológico , Adulto , Carbolinas/uso terapéutico , Esquema de Medicación , Humanos , Masculino , Persona de Mediana Edad , Inhibidores de Fosfodiesterasa/administración & dosificación , Inhibidores de Fosfodiesterasa/uso terapéutico , Estudios Prospectivos , Tadalafilo , Resultado del Tratamiento
10.
J AOAC Int ; 96(4): 897-901, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24000766

RESUMEN

A method based on HPLC with UV detection was developed for the quantitative determination of chloramphenicol (CAP) residues in aquatic products. The samples were extracted with ethyl acetate-ammonium hydroxide (98 + 2, v/v), followed by a cleanup step using an immunoaffinity column. The analytes were determined by HPLC-UV. Optimal conditions for the extraction and cleanup procedures are described. The linear regression equation was y = 91.47x - 8.60 with R = 0.9998 (y = peak area and x = CAP concentration) and showed a good reproducibility. The LOQ was 0.25 microg/kg for determining CAP spiked in the aquatic products. The mean recoveries of CAP from fish and shrimp samples fortified at 0.25-1.0 microg/kg were 88.7-93.1 and 92.0-97.3%, respectively; the repeatability RSDs were less than 8.1%. It was concluded that the method is simple, highly sensitive, and low cost for quantitatively measuring CAP residues in aquatic products. Analyte identification was confirmed by HPLC/MSIMS analysis.


Asunto(s)
Antibacterianos/análisis , Cloranfenicol/análisis , Cromatografía de Afinidad/métodos , Cromatografía Líquida de Alta Presión/métodos , Residuos de Medicamentos/análisis , Alimentos Marinos/análisis , Espectrofotometría Ultravioleta
11.
Artículo en Inglés | MEDLINE | ID: mdl-23845391

RESUMEN

A multi-residue analysis method for simultaneous determination of nine subclasses of non-steroidal anti-inflammatory drugs (NSAIDs) in milk and dairy products by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been established. The sample was initially extracted and deproteinized with ascorbic acid buffer (0.01M, pH 3) and acetonitrile-ethyl acetate mixture, followed by centrifugation and evaporation, then reconstituted with acetonitrile-0.1% formic acid (1+1, v/v). After removal of lipid material by n-hexane, the sample was analyzed by UPLC-MS/MS with electro-spray ionization (ESI) interface in Multiple Reaction Monitoring (MRM) mode. The range of limits of detection (LODs) and limits of quantification (LOQs) were 0.03-0.30µg/kg and 0.10-1.00µg/kg, respectively. The recoveries in milk, milk powder, yogurt, processed cheese and milk beverage ranged from 61.7% to 117%, and the relative standard deviations (RSDs) were less than 17.9% at three spiked levels (1, 10 and 100 times of the LOQ). Matrix effects were also investigated and it was determined the signals of the analytes were suppressed from 9.4% to 76.6% in processed cheese. The proposed method was also applied to incurred sample analysis. The results proved that this method was suitable for the simultaneous determination of nine subclasses of NSAIDs residues in milk and dairy products.


Asunto(s)
Antiinflamatorios no Esteroideos/química , Cromatografía Líquida de Alta Presión/métodos , Productos Lácteos/análisis , Residuos de Medicamentos/química , Leche/química , Espectrometría de Masas en Tándem/métodos , Animales , Bovinos , Contaminación de Alimentos/análisis , Límite de Detección , Espectrometría de Masa por Ionización de Electrospray/métodos
12.
Artículo en Inglés | MEDLINE | ID: mdl-23286983

RESUMEN

A high performance liquid chromatography method with visible detection (HPLC-VIS) for the determination of malachite green (MG), crystal violet (CV), leucomalachite green (LMG), and leucocrystal violet (LCV) in fish has been developed after clean-up through an immunoaffinity column (IAC). Residues were simultaneously extracted from fish muscle with acetonitrile and ammonium acetate buffer. The leuco-forms, LMG and LCV, were oxidized quantitatively to the chromic CV and MG by reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Extracts were then purified on an IAC which prepared by immobilizing the anti-MG-CV antibodies by the sol-gel method. Finally, the eluents were analyzed by HPLC-VIS. The limits of detection were 0.15, 0.1, 0.18 and 0.14ng/g for MG, CV, LMG and LCV, respectively. The average recoveries in samples fortified with MG, CV, LMG and LCV over the range 0.5-10ng/g were from 71.6% to 96.8% with RSDs of 5.1-12.3% (n=6). This novel method was confirmed by liquid chromatography-tandem mass spectrometry with electrospray interface in positive mode using multiple reaction monitoring.


Asunto(s)
Cromatografía de Afinidad/métodos , Cromatografía Líquida de Alta Presión/métodos , Peces , Violeta de Genciana/análisis , Colorantes de Rosanilina/análisis , Acetonitrilos/química , Animales , Límite de Detección , Músculos/química , Oxidación-Reducción , Reproducibilidad de los Resultados
13.
Shanghai Kou Qiang Yi Xue ; 21(3): 308-11, 2012 Jun.
Artículo en Chino | MEDLINE | ID: mdl-22885493

RESUMEN

PURPOSE: To determine the bone absorption at different time after alveolar autogenous bone grafting using spiral CT three-dimensional reconstruction. METHODS: Twenty unilateral cleft lip and palate patients(15 males,5 females) were chosen to undergo three-dimensional CT scans after alveolar bone graft, region of interest(ROI) volume measurement of GE AW 4.1 software was used to calculate the capacity of alveolar cleft at 1 week, 3 months, 6 months after surgery. RESULTS: An average bone loss of 35.74% was found after 3 months, 55.89% after 6 months. In labial and palatal direction, labial bony bridge was more obvious than palatal bony bridge. CONCLUSIONS: Bone resorption is present after alveolar bone graft, and becomes more severe with time going on.


Asunto(s)
Injerto de Hueso Alveolar , Fisura del Paladar , Proceso Alveolar , Trasplante Óseo , Labio Leporino , Femenino , Humanos , Masculino , Tomografía Computarizada Espiral , Tomografía Computarizada por Rayos X
14.
Oncol Lett ; 3(4): 791-796, 2012 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-22740995

RESUMEN

Cancer stem cells (CSCs) that exhibit tumor-initiating properties have been identified in primary prostate cancer and prostate cancer cell lines. CSCs are the root of tumor metastasis and recurrence even in the cases where they constitute a minority of the tumor mass. In this study, putative CSCs were isolated from the Du145 cell line and an Affymetrix microarray was used to investigate their gene expression profile. The results were validated by real-time polymerase chain reaction (PCR). The results of the microarray indicated that 138 genes were upregulated and 93 genes were downregulated in the CSCs. Certain genes that may be significant in the regulation of CSCs from the Du145 cell line were identified. These results may aid the studying of the mechanisms through which CSCs acquire their distinctive properties.

15.
Stem Cells Dev ; 21(18): 3372-80, 2012 Dec 10.
Artículo en Inglés | MEDLINE | ID: mdl-22731611

RESUMEN

Androgen-independent prostate cancer (AIPC) is difficult to treat. Present study is to explore the inhibitory effect of a cytokine environment on AIPC and its mechanism. We utilized nerve growth factor (NGF)/γ-interferon (γ-IFN) to change the cytokine environment. Animal models and 2 androgen receptor (AR)-negative prostate cancer cell lines were used to evaluate the effect of NGF/γ-IFN. Flow cytometry, immunocytochemistry, western blotting, Tunel assay, colony formation efficiency, gene microarray, and in vivo bioluminescence were used to discern the mechanisms within NGF/γ-IFN that effect the environment. In vitro, NGF/γ-IFN effectively inhibited the proliferation of AIPC cell lines and promoted the apoptosis of the cancer cells. In vivo, NGF/γ-IFN suppressed the growth and metastasis of a tumor mass that arose from the AIPC cell line. After NGF/γ-IFN treatment, the AR-negative cell lines re-expressed AR and were then able to respond to the androgen. Contrary to expectations, the proliferation of cells was inhibited after dihydrotestosterone was added, and the results indicated that NGF/γ-IFN decreased the proportion of cancer stem cells. NGF/γ-IFN worked mainly through the downregulation of fibroblast growth factor receptor 2.


Asunto(s)
Interferón gamma/farmacología , Factor de Crecimiento Nervioso/farmacología , Neoplasias de la Próstata/tratamiento farmacológico , Receptor Tipo 2 de Factor de Crecimiento de Fibroblastos/biosíntesis , Receptores Androgénicos/metabolismo , Animales , Apoptosis , Recuento de Células , Línea Celular Tumoral , Proliferación Celular , Dihidrotestosterona/farmacología , Regulación hacia Abajo , Humanos , Interferón gamma/metabolismo , Masculino , Ratones , Ratones Endogámicos NOD , Ratones SCID , Metástasis de la Neoplasia , Trasplante de Neoplasias , Células Madre Neoplásicas/citología , Factor de Crecimiento Nervioso/metabolismo , Receptores Androgénicos/efectos de los fármacos , Trasplante Heterólogo , Proteína Wnt1/biosíntesis , Proteína wnt2/biosíntesis
16.
Shanghai Kou Qiang Yi Xue ; 21(2): 180-4, 2012 Apr.
Artículo en Chino | MEDLINE | ID: mdl-22610329

RESUMEN

PURPOSE: To establish a new method of presurgical alveolar molding using computer aided design(CAD) in infants with complete unilateral cleft lip and palate (UCLP). METHODS: Ten infants with complete UCLP were recruited. A maxillary impression was taken at the first examination after birth. The study model was scanned by a non-contact three-dimensional laser scanner and a digital model was constructed and analyzed to simulate the alveolar molding procedure with reverse engineering software (RapidForm 2006). The digital geometrical data were exported to produce a scale model using rapid prototyping technology. The whole set of appliances was fabricated based on these solid models. RESULTS: The digital model could be viewed and measured from any direction by the software. By the end of the NAM treatment before surgical lip repair, the cleft was narrowed and the malformation of alveolar segments was aligned normally, significantly improving nasal symmetry and nostril shape. CONCLUSIONS: Presurgical NAM using CAD could simplify the treatment procedure and estimate the treatment objective, which enabled precise control of the force and direction of the alveolar segments movement.


Asunto(s)
Labio Leporino , Fisura del Paladar , Diseño Asistido por Computadora , Humanos , Lactante , Maxilar , Nariz
17.
Zhonghua Yi Xue Za Zhi ; 92(2): 110-3, 2012 Jan 10.
Artículo en Chino | MEDLINE | ID: mdl-22490693

RESUMEN

OBJECTIVE: To explore the clinical features and related factors of sexual dysfunctions in male patients with pituitary adenomas. METHODS: The questionnaires of sexual functions were collected from 86 male patients with pituitary adenomas. We examined the clinical features of sexual dysfunctions and analyzed the correlations between sexual behaviors and age, tumor type, invasiveness, tumor size, serum levels of prolactin (PRL) and testosterone. RESULTS: The incidence of sexual dysfunctions was 80.2% (69/86). Sexual dysfunctions were found in 84.6% (66/78) of the patients with functioning pituitary adenomas and 37.5% (3/8) of those with non-functioning pituitary adenoma respectively. In the PRL group, the incidence of erectile dysfunctions was 92.1% (35/38) and it was higher than those in the FSH (follicle-stimulating hormone) and GH (growth hormone) groups (P < 0.05). In the FSH group, the incidence of reduced sexual desire was 78.3% (18/23). In the GH group, the incidence of erectile dysfunctions was 70.6% (12/17) and the incidence of reduced sexual desire or ejaculation dysfunction was lower than that of the PRL/FSH group (P < 0.05). CONCLUSION: The incidence of sexual dysfunctions is quite high in males with pituitary adenomas, especially for those with functioning pituitary adenomas. The clinical features of sexual dysfunctions vary in different types of functioning pituitary adenoma. The incidence of erectile dysfunctions is the highest in the PRL group. Pathological type of pituitary tumors is a major risk factor of sexual dysfunctions.


Asunto(s)
Disfunción Eréctil/epidemiología , Neoplasias Hipofisarias/epidemiología , Adulto , Humanos , Masculino , Persona de Mediana Edad , Neoplasias Hipofisarias/fisiopatología , Estudios Retrospectivos , Adulto Joven
18.
J Chromatogr A ; 1219: 104-13, 2012 Jan 06.
Artículo en Inglés | MEDLINE | ID: mdl-22142970

RESUMEN

An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC-MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC-ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 µg/kg and 2.0 µg/kg, and limits of quantification were between 1.0 µg/kg and 5.0 µg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0-500 µg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.


Asunto(s)
Antiinflamatorios no Esteroideos/análisis , Cromatografía Líquida de Alta Presión/métodos , Residuos de Medicamentos/análisis , Músculos/química , Espectrometría de Masas en Tándem/métodos , Acetonitrilos , Animales , Análisis de los Alimentos , Límite de Detección , Modelos Lineales , Ácidos Fosfóricos , Reproducibilidad de los Resultados , Porcinos
19.
BMC Cancer ; 11: 297, 2011 Jul 14.
Artículo en Inglés | MEDLINE | ID: mdl-21756352

RESUMEN

BACKGROUND: Since transitional cell carcinoma (TCC) of the upper urinary tract is a relatively uncommon malignancy, the role of adjuvant radiotherapy is unknown. METHODS: We treated 133 patients with TCC of the renal pelvis or ureter at our institution between 1998 and 2008. The 67 patients who received external beam radiotherapy (EBRT) following surgery were assigned to the radiation group (RT). The clinical target volume included the renal fossa, the course of the ureter to the entire bladder, and the paracaval and para-aortic lymph nodes, which were at risk of harbouring metastatic disease in 53 patients. The tumour bed or residual tumour was targeted in 14 patients. The median radiation dose administered was 50 Gy. The 66 patients who received intravesical chemotherapy were assigned to the non-radiation group (non-RT). RESULTS: The overall survival rates for the RT and non-RT groups were not significantly different (p = 0.198). However, there was a significant difference between the survival rates for these groups based on patients with T3/T4 stage cancer. A significant difference was observed in the bladder tumour relapse rate between the irradiated and non-irradiated bladder groups (p = 0.004). Multivariate analysis indicated that improved overall survival was associated with age < 60 years, T1 or T2 stage, absence of synchronous LN metastases, and EBRT. Acute gastrointestinal and bladder reactions were the most common symptoms, but mild non-severe (> grade 3) hematologic symptoms also occurred. CONCLUSION: EBRT may improve overall survival for patients with T3/T4 cancer of the renal pelvis or ureter and delay bladder tumour recurrence in all patients.


Asunto(s)
Carcinoma de Células Transicionales/radioterapia , Pelvis Renal/efectos de la radiación , Uréter/efectos de la radiación , Neoplasias de la Vejiga Urinaria/radioterapia , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Anorexia/etiología , Carcinoma de Células Transicionales/tratamiento farmacológico , Carcinoma de Células Transicionales/cirugía , Terapia Combinada , Femenino , Estudios de Seguimiento , Humanos , Estimación de Kaplan-Meier , Pelvis Renal/efectos de los fármacos , Pelvis Renal/cirugía , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/prevención & control , Estadificación de Neoplasias , Pronóstico , Radioterapia/efectos adversos , Radioterapia/métodos , Dosificación Radioterapéutica , Resultado del Tratamiento , Uréter/efectos de los fármacos , Uréter/cirugía , Neoplasias de la Vejiga Urinaria/tratamiento farmacológico , Neoplasias de la Vejiga Urinaria/cirugía
20.
Asian J Androl ; 13(3): 499-504, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21399650

RESUMEN

The aim of this study was to investigate the feasibility and safety of high-intensity focused ultrasound (HIFU) combined with (+) low-dose external beam radiotherapy (LRT) as supplemental therapy for advanced prostate cancer (PCa) following hormonal therapy (HT). Our definition of HIFU+LRT refers to treating primary tumour lesions with HIFU in place of reduced field boost irradiation to the prostate, while retaining four-field box irradiation to the pelvis in conventional-dose external beam radiotherapy (CRT). We performed a prospective, controlled and non-randomized study on 120 patients with advanced PCa after HT who received HIFU, CRT, HIFU+LRT and HT alone, respectively. CT/MR imaging showed the primary tumours and pelvic lymph node metastases visibly shrank or even disappeared after HIFU+LRT treatment. There were significant differences among four groups with regard to overall survival (OS) and disease-specific survival (DSS) curves (P = 0.018 and 0.015). Further comparison between each pair of groups suggested that the long-term DSS of the HIFU+LRT group was higher than those of the other three groups, but there was no significant difference between the HIFU+LRT group and the CRT group. Multivariable Cox's proportional hazard model showed that both HIFU+LRT and CRT were independently associated with DSS (P = 0.001 and 0.035) and had protective effects with regard to the risk of death. Compared with CRT, HIFU+LRT significantly decreased incidences of radiation-related late gastrointestinal (GI) and genitourinary (GU) toxicity grade ≥ II. In conclusion, long-term survival of patients with advanced PCa benefited from strengthening local control of primary tumour and regional lymph node metastases after HT. As an alternative to CRT, HIFU+LRT showed good efficacy and better safety.


Asunto(s)
Neoplasias de la Próstata/terapia , Terapia por Ultrasonido , Anciano , Terapia Combinada , Humanos , Masculino , Próstata/patología , Próstata/efectos de la radiación , Antígeno Prostático Específico/metabolismo , Neoplasias de la Próstata/tratamiento farmacológico , Neoplasias de la Próstata/radioterapia , Radioterapia Conformacional/métodos
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