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1.
Primates ; 64(6): 589-594, 2023 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-37555863

RESUMEN

Predation is widely recognized as a powerful selective pressure on primate behavior and ecology, although knowledge of predator-prey relationships remains limited partly due to the rarity of directly observed attacks on primates. Here, we describe four confirmed or suspected instances of leopard (Panthera pardus) predation on free-ranging Sichuan (golden) snub-nosed monkeys (Rhinopithecus roxellana), a highly endangered colobine species endemic to China. We recorded predation events and the reactions of monkey group members. We suggest that the evolution of a multilevel society may be an adaptive response by Sichuan snub-nosed monkeys to the risk from leopards as well as other potential predators, one that balances the pressures of predation and intra-species competition and conflict.


Asunto(s)
Colobinae , Panthera , Presbytini , Animales , Conducta Predatoria , Colobinae/fisiología , China
2.
RSC Adv ; 13(30): 21088-21095, 2023 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-37448635

RESUMEN

Fluorescent carbon nanomaterials have attracted increasing attention owing to their unique photoluminescence properties, good biocompatibility and low toxicity in bioimaging as well as biosensing. Heteroatom doping is usually used to improve photoluminescence properties by tuning the functional groups and the particle size domain effect, thus leading to redshifted emission. Here, we report a straightforward strategy for the fabrication of a mixture of fluorescent phosphorus and nitrogen carbon nanodots (P,N-CDs) followed by separating two kinds of fluorescent fractions based on their different negative charges. Such a one-pot hydrothermal method using formamide, urea and hydroxyethylidene diphosphonic acid as the precursor yields fluorescent P,N-CDs. Specifically, blue-emitting CDs (bCDs) and green-emitting CDs (gCDs) were separated by using column chromatography. The quantum yields of bCDs and gCDs were 20.33% and 1.92%, respectively. And the fluorescence lifetimes of bCDs and gCDs were 6.194 ns and 2.09 ns, respectively. What is more, the resultant P,N-CDs exhibited low toxicity and excellent biocompatibility. Confocal fluorescence microscopy images were obtained successfully, suggesting that P,N-CDs have excellent cell membrane permeability and cellular imaging. This work provides a promising fluorescent carbon nanomaterial with tunable emission as a probe for versatile applications in bioimaging, sensing and drug delivery.

4.
Artículo en Inglés | MEDLINE | ID: mdl-35742458

RESUMEN

This study investigated the single-pass performance of a negative corona electrostatic precipitators (ESP) in removing suspended particulates (PM2.5 and PM10), formaldehyde (HCHO), and bioaerosols (bacteria and fungi) and measured the ozone (O3) concentration generated by ESP. The experimental results revealed that if the operational conditions for the ESP were set to high voltage (-10.5 kV) and low air flow rate (2.4 m3/min), ESP had optimal air pollutant removal efficiency. In the laboratory system, its PM2.5 and PM10 removal rates both reached 99% at optimal conditions, and its HCHO removal rate was 55%. In field tests, its PM2.5, PM10, HCHO, bacteria, and fungi removal rates reached 89%, 90%, 46%, 69%, and 85% respectively. The ESP in the laboratory system (-10.5 kV and 2.4 m3/min) generated 7.374 ppm of O3 under optimal conditions. Under the same operational conditions, O3 generated by ESP in the food waste storage room and the meeting room were 1.347 ppm and 1.749 ppm, respectively. The removal of HCHO and bioaerosols was primarily attributed to their destruction in the corona, as well as ozone oxidation, and collection on the dust collection plate.


Asunto(s)
Contaminantes Atmosféricos , Contaminación del Aire Interior , Ozono , Eliminación de Residuos , Contaminantes Atmosféricos/análisis , Alimentos , Formaldehído , Ozono/análisis , Material Particulado/análisis , Electricidad Estática
5.
Plant Signal Behav ; 16(9): 1929731, 2021 09 02.
Artículo en Inglés | MEDLINE | ID: mdl-34092178

RESUMEN

Endophytic fungi usually establish a symbiotic relationship with the host plant and affect its growth. In order to evaluate the impact of endophytic fungi on the Chinese herbal medicinal plant Houttuynia cordata Thunb., three endophytes isolated from the rhizomes of H. cordata, namely Ilyonectria liriodendra (IL), unidentified fungal sp. (UF), and Penicillium citrinum (PC), were co-cultured individually with H. cordata in sterile soil for 60 days. Analysis of the results showed that the endophytes stimulated the host plant in different ways: IL increased the growth of rhizomes and the accumulation of most of the phenolics and volatiles, UF promoted the accumulation of the medicinal compounds afzelin, decanal, 2-undecanone, and borneol without influencing host plant growth, and PC increased the fresh weight, total leaf area and height of the plants, as well as the growth of the rhizomes, but had only a small effect on the concentration of major secondary metabolites. Our results proved that the endophytic fungi had potential practical value in terms of the production of Chinese herbal medicines, having the ability to improve the yield and accumulation of medicinal metabolites.


Asunto(s)
Endófitos/metabolismo , Houttuynia/química , Houttuynia/crecimiento & desarrollo , Houttuynia/microbiología , Rizoma/crecimiento & desarrollo , Rizoma/metabolismo , Rizoma/microbiología , Hypocreales/metabolismo , Penicillium/metabolismo , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Plantas Medicinales/química , Plantas Medicinales/crecimiento & desarrollo , Plantas Medicinales/microbiología , Simbiosis
6.
J Neurotrauma ; 38(9): 1203-1224, 2021 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-33292072

RESUMEN

The blood-spinal cord barrier (BSCB), a physical barrier between the blood and spinal cord parenchyma, prevents the toxins, blood cells, and pathogens from entering the spinal cord and maintains a tightly controlled chemical balance in the spinal environment, which is necessary for proper neural function. A BSCB disruption, however, plays an important role in primary and secondary injury processes related to spinal cord injury (SCI). After SCI, the structure of the BSCB is broken down, which leads directly to leakage of blood components. At the same time, the permeability of the BSCB is also increased. Repairing the disruption of the BSCB could alleviate the SCI pathology. We review the morphology and pathology of the BSCB and progression of therapeutic methods targeting BSCB in SCI.


Asunto(s)
Barrera Hematoencefálica/metabolismo , Endotelio Vascular/metabolismo , Células-Madre Neurales/metabolismo , Traumatismos de la Médula Espinal/metabolismo , Médula Espinal/metabolismo , Animales , Barrera Hematoencefálica/patología , Movimiento Celular/fisiología , Endotelio Vascular/patología , Humanos , Médula Espinal/patología , Traumatismos de la Médula Espinal/patología , Traumatismos de la Médula Espinal/terapia
7.
Medicine (Baltimore) ; 99(22): e20369, 2020 May 29.
Artículo en Inglés | MEDLINE | ID: mdl-32481419

RESUMEN

BACKGROUND: This study aims to explore the efficacy and safety of prostaglandina E1 (PE1) for the treatment of patients with thrombo-occlusive vasculitis (TOV). METHODS: Electronic databases (Cochrane Library, PUBMED, EMBASE, Web of Science, Scopus, the Allied and Complementary Medicine Database, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure) will be sought from onset to the March 1, 2020 without language and publication status restrictions. We will include any potential randomized controlled trials that examined the efficacy of PE1 for the treatment of patients with TOV. We will appraise study quality using Cochrane risk of bias tool, and will assess the evidence quality using Grading of Recommendations Assessment Development and Evaluation. We will use RevMan 5.3 Software for statistical analysis. RESULTS: A high-quality synthesis of present evidence of PE1 for the treatment of patients with TOV will be provided in this study. CONCLUSION: This study will provide evidence to judge whether PE1 is an effective intervention for TOV. SYSTEMATIC REVIEW REGISTRATION: INPLASY202040081.


Asunto(s)
Alprostadil/uso terapéutico , Trombosis/complicaciones , Vasculitis/tratamiento farmacológico , Humanos , Resultado del Tratamiento , Vasculitis/etiología , Metaanálisis como Asunto
8.
Cell Biol Int ; 44(8): 1714-1726, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32339345

RESUMEN

The dysregulation of proliferation and migration of vascular smooth muscle cells (VSMCs) contributes to atherosclerosis (AS) and accumulating reports indicate the crucial role of long noncoding RNA in AS. However, the role of small nucleolar RNA host gene 12 (SNHG12) in regulating the phenotypes of VSMCs and AS remains largely unknown. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to detect the expression levels of SNHG12 and miR-199a-5p in an in vivo AS model and VSMCs treated by oxidized low-density lipoprotein (ox-LDL). The proliferation ability, migration ability, and apoptosis of VSMCs were tested by cell counting kit-8, Transwell assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay, respectively. StarBase database was used to predict the binding sites between miR-199a-5p and SNHG12. The interaction between miR-199a-5p and SNHG12 was validated by qRT-PCR, western blot, and luciferase reporter assay. Western blot was used to examine the effects of SNHG12 and miR-199a-5p on the expression of hypoxia-inducible factor 1α (HIF-1α). We found that the expression level of SNHG12 was significantly increased in the animal model and VSMCs treated by ox-LDL. Knockdown of SNHG12 suppressed the proliferation and migration abilities of VSMCs, while overexpression of SNHG12 had the opposite effects. Mechanically, we validated that miR-199a-5p was a target of SNHG12, and the target gene of miR-199a-5p, HIF-1α could be indirectly and positively regulated by SNHG12. In conclusion, SHNG12 targeting miR-199a-5p/HIF-1α contributed to the pathophysiological process of AS by regulating the phenotypes of VSMCs, and could be a potential therapy target for this disease.


Asunto(s)
Aterosclerosis/genética , Regulación de la Expresión Génica , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , MicroARNs/metabolismo , Músculo Liso Vascular/metabolismo , ARN Largo no Codificante/metabolismo , Animales , Apoptosis , Aterosclerosis/metabolismo , Arterias Carótidas/citología , Movimiento Celular , Proliferación Celular , Células Cultivadas , Subunidad alfa del Factor 1 Inducible por Hipoxia/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Músculo Liso Vascular/citología
9.
J Glob Antimicrob Resist ; 20: 160-162, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-31877398

RESUMEN

OBJECTIVES: Recently, the Gram-negative bacterium Klebsiella oxytoca has been identified as an emerging pathogen. Here we report the draft genome of a 2,3-butanediol-producing strain, K. oxytoca CCTCC M207023, isolated from soil in Nanjing, China. The tetracycline-resistant phenotype and the high yield of 2,3-butanediol was demonstrated. METHODS: The draft genome of K. oxytoca CCTCC M207023 was determined using an Illumina NovaSeq™ 6000 next-generation DNA sequencing platform. Clean sequencing data were subsequently assembled using SOAPdenovo. RESULTS: The draft genome of K. oxytoca CCTCC M207023, comprising 5 658 144bp and with a GC content of 56.50%, was assembled into 5262 open-reading frames (ORFs). Antimicrobial resistance genes were also annotated. CONCLUSIONS: The draft genome sequence of K. oxytoca CCTCC M207023 reported here will be a reference for comparative analysis with the antimicrobial resistance mechanisms for the safety of 2,3-butanediol industrial production.


Asunto(s)
Butileno Glicoles/metabolismo , Farmacorresistencia Bacteriana Múltiple , Klebsiella oxytoca/genética , Secuenciación Completa del Genoma/métodos , Proteínas Bacterianas/genética , Composición de Base , China , Genoma Bacteriano , Secuenciación de Nucleótidos de Alto Rendimiento , Klebsiella oxytoca/metabolismo , Sistemas de Lectura Abierta , Tetraciclina/farmacología , Factores de Virulencia/genética
10.
Chin Med J (Engl) ; 132(21): 2601-2611, 2019 Nov 05.
Artículo en Inglés | MEDLINE | ID: mdl-31373906

RESUMEN

BACKGROUND: In consideration of characteristics and functions, extra-cellular signal-regulated protein kinase 5 (ERK5) signaling pathway could be a new target for spinal cord injury (SCI) treatment. Our study aimed to evaluate the roles of ERK5 signaling pathway in secondary damage of SCI. METHODS: We randomly divided 70 healthy Wistar rats into five groups: ten in the blank group, 15 in the sham surgery + BIX02188 (sham + B) group, 15 in the sham surgery + dimethyl sulfoxide (DMSO; sham + D) group, 15 in the SCI + BIX02188 (SCI + B) group, and 15 in the SCI + DMSO (SCI + D) group. BIX02188 is a specific inhibitor of the ERK5 signaling pathway. SCI was induced by the application of vascular clips (with the force of 30 g) to the dura on T10 level, while rats in the sham surgery group underwent only T9-T11 laminectomy. BIX02188 or DMSO was intra-thecally injected at 1, 6, and 12 h after surgery or SCI. Spinal cord samples were taken for testing at 24 h after surgery or SCI. RESULTS: Expression of phosphorylated-ERK5 (p-ERK5) significantly increased after SCI. Application of BIX02188 indeed inhibited ERK5 signaling pathway and reduced the degree of spinal cord tissue injury, neutrophil infiltration and proinflammatory cytokine expression, nuclear factor-κB (NF-κB) activation and apoptosis (measured by TdT-mediated 2'-deoxyuridine 5'-triphosphate nick-end labeling, expression of Fas-ligand, BCL2-associated X [Bax], and B-cell lymphoma-2 [Bcl-2]). Double immunofluorescence revealed activation of ERK5 in neurons and microglia after SCI. CONCLUSION: ERK5 signaling pathway was activated in spinal neurons and microglia, contributing to secondary injury of SCI. Moreover, inhibition of ERK5 signaling pathway could alleviate the degree of SCI, which might be related to its regulation of infiltration of inflammatory cells and release of inflammatory cytokines, expression of NF-κB and cell apoptosis.


Asunto(s)
Proteína Quinasa 7 Activada por Mitógenos/metabolismo , Traumatismos de la Médula Espinal/metabolismo , Animales , Apoptosis/fisiología , Masculino , FN-kappa B/metabolismo , Ratas , Ratas Wistar , Transducción de Señal/fisiología
11.
Appl Microbiol Biotechnol ; 103(11): 4313-4324, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31016357

RESUMEN

In recent years, eukaryotic microorganisms have been widely applied to offer many solutions for everyday life and have come to play important roles in agriculture, food, health care, and the fine-chemicals industry. However, the complex genetic background and low homologous recombination efficiency have hampered the implementation of large-scale and high-throughput gene editing in many eukaryotic microorganisms. The low efficiency of homologous recombination (HR) not only makes the modification process labor-intensive but also completely precludes the application of many otherwise very useful genome editing techniques. Thus, increasing the efficiency of HR is clearly an enabling technology for basic research and gene editing in eukaryotic microorganisms. In this review, we summarize the current strategies for enhancing the efficiency of HR in eukaryotic microorganisms (particularly yeasts and filamentous fungi), list some small molecules and candidate genes associated with homologous and non-homologous recombination, and briefly discuss the further development prospects of these strategies.


Asunto(s)
Hongos/genética , Edición Génica/métodos , Recombinación Homóloga , Ingeniería Metabólica/métodos , Levaduras/genética
12.
Bioresour Technol ; 281: 449-456, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-30846235

RESUMEN

Terpenoids are a large class of natural compounds based on the C5 isoprene unit, with many biological effects such activity against cancer and allergies, while some also have an agreeable aroma. Consequently, they have received extensive attention in the food, pharmaceutical and cosmetic fields. With the identification and analysis of the underlying natural product synthesis pathways, current microbial-based metabolic engineering approaches have yielded new strategies for the production of highly valuable terpenoids. Yarrowia lipolytica is a non-conventional oleaginous yeast that is rapidly emerging as a valuable host for the production of terpenoids due to its own endogenous mevalonate pathway and high oil production capacity. This review aims to summarize the status and strategies of metabolic engineering for the heterologous synthesis of terpenoids in Y. lipolytica in recent years and proposes new methods aiming towards further improvement of terpenoid production.


Asunto(s)
Ingeniería Metabólica/métodos , Terpenos/metabolismo , Yarrowia/metabolismo , Productos Biológicos/metabolismo
13.
ACS Synth Biol ; 8(2): 445-454, 2019 02 15.
Artículo en Inglés | MEDLINE | ID: mdl-30616338

RESUMEN

The filamentous fungus Fusarium fujikuroi is well-known for its production of natural plant growth hormones: a series of gibberellic acids (GAs). Some GAs, including GA1, GA3, GA4, and GA7, are biologically active and have been widely applied in agriculture. However, the low efficiency of traditional genetic tools limits the further research toward making this fungus more efficient and able to produce tailor-made GAs. Here, we established an efficient CRISPR/Cas9-based genome editing tool for F. fujikuroi. First, we compared three different nuclear localization signals (NLS) and selected an efficient NLS from histone H2B (HTBNLS) to enable the import of the Cas9 protein into the fungal nucleus. Then, different sgRNA expression strategies, both in vitro and different promoter-based in vivo strategies, were explored. The promoters of the U6 small nuclear RNA and 5S rRNA, which were identified in F. fujikuroi, had the highest editing efficiency. The 5S rRNA-promoter-driven genome editing efficiency reached up to 79.2%. What's more, multigene editing was also explored and showed good results. Finally, we used the developed genome editing tool to engineer the metabolic pathways responsible for the accumulation of a series GAs in the filamentous fungus F. fujikuroi, and successfully changed its GA product profile, from GA3 to tailor-made GA4 and GA7 mixtures. Since these mixtures are more efficient for agricultural use, especially for fruit growth, the developed strains will greatly improve industrial GA production.


Asunto(s)
Sistemas CRISPR-Cas/genética , Hongos/genética , Hongos/metabolismo , Fusarium/genética , Fusarium/metabolismo , Giberelinas/metabolismo , Edición Génica/métodos , Genoma Fúngico/genética
15.
Medicine (Baltimore) ; 97(27): e11140, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29979380

RESUMEN

This study aims to explore the influence of bone resorption of the spinous process after single-segment interspinous process device (IPD) implantation on the biomechanics of the lumbar spine.The 3D finite element model of the lumbar spine (L3-L5) was modified, and 2 models that simulated the presence and absence of bone resorption of the spinous process were developed using an IPD (Wallis). Its biomechanical effects, such as change in range of motion (ROM) and intervertebral disc and facet stress, were introduced at operative (L4/5) and adjacent (L3/4) levels.Compared with the INT model, the Wallis model and Wallis-BR model had similar ROMs in lateral flexion and rotation. However, the Wallis model had a lower L3-5 ROM in flexion (20.4% lower) and extension (26.4% lower), and L4-L5 ROM in flexion (74.1% lower) and extension (70.8% lower), while the overall ROM of the Wallis-BR model was greater than that of the Wallis model. The stress on the L3/L4 intervertebral disc and facets was similar for all 3 models. Compared with the INT model and Wallis-BR model, the stress on the L4/L5 intervertebral disc and facets under all movements significantly decreased in the Wallis model. The stress on the L5 process was greater than that on the L4 process in both the Wallis model and Wallis-BR model, and the load on the processes that underwent bone resorption was lower than that of the Wallis model.The function of the IPD slowly decreased with the occurrence of bone resorption of the interspinous process. This bone remodeling may be associated with high stress after IPD implantation.


Asunto(s)
Fenómenos Biomecánicos/fisiología , Resorción Ósea/diagnóstico por imagen , Vértebras Lumbares/diagnóstico por imagen , Vértebras Lumbares/cirugía , Fusión Vertebral/instrumentación , Estenosis Espinal/diagnóstico por imagen , Adulto , Remodelación Ósea/fisiología , Resorción Ósea/fisiopatología , Femenino , Análisis de Elementos Finitos , Humanos , Disco Intervertebral/fisiología , Vértebras Lumbares/fisiopatología , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Rango del Movimiento Articular/fisiología , Fusión Vertebral/efectos adversos , Estenosis Espinal/cirugía , Tomografía Computarizada por Rayos X , Resultado del Tratamiento
16.
World Neurosurg ; 113: e70-e76, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29408574

RESUMEN

OBJECTIVE: To assess the mid-long-term follow-up of the safety and efficacy of anterior cervical discectomy and fusion (ACDF), cervical artificial disc replacement (CADR) and hybrid surgery (HS) for bilevel cervical degenerative disc disease (cDDD). METHODS: 77 patients who underwent ACDF, HS, and CADR were retrospectively reviewed. Clinical effects were evaluated based on Neck Disability Index (NDI), Visual Analog Scale (VAS), and Japanese Orthopedic Association (JOA) scores and the Odom criteria. Radiographic outcomes were evaluated, including cervical range of motion (ROM), ROM in the operative and adjacent segments, incidence of degeneration in the adjacent segments (ASD), and heterotopic ossification (HO). RESULTS: NDI, VAS, and JOA scores significantly improved in all patients after surgery without significant differences between groups. The excellent-to-good ratio in the Odom scale was 28/30 for the HS group, 30/33 for the ACDF group, and 13/14 for the CADR group. No significant differences in clinical outcomes or complication were found between groups (P > 0.05). Furthermore, the HS and CADR groups had less decreased ROM in the cervical and operative segments and less compensatory ROM in adjacent segments (P < 0.05). By contrast, the ACDF group had decreased ROM in the cervical and operative segments and significantly increased ROM in adjacent segments (P < 0.05). Moreover, the incidence of ASD was higher in the ACDF group, but the difference was not statistically significant (P > 0.05). HO was found in 10 patients (33.3%) in the HS group and 5 patients (35.7%) in the CADR group. CONCLUSION: HS was superior to ACDF with regard to equivalent clinical outcomes in the mid-long-term follow-up. Furthermore, HS was superior in the maintenance of ROM and had less impact on its adjacent segments. The efficacy of HS is similar to that of CADR.


Asunto(s)
Vértebras Cervicales/cirugía , Discectomía/métodos , Degeneración del Disco Intervertebral/cirugía , Fusión Vertebral/métodos , Reeemplazo Total de Disco/métodos , Anciano , Vértebras Cervicales/diagnóstico por imagen , Vértebras Cervicales/patología , Progresión de la Enfermedad , Femenino , Estudios de Seguimiento , Humanos , Degeneración del Disco Intervertebral/complicaciones , Degeneración del Disco Intervertebral/diagnóstico por imagen , Degeneración del Disco Intervertebral/patología , Masculino , Persona de Mediana Edad , Dolor de Cuello/etiología , Osificación Heterotópica/epidemiología , Osificación Heterotópica/etiología , Dimensión del Dolor , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/etiología , Prótesis e Implantes , Rango del Movimiento Articular , Estudios Retrospectivos , Índice de Severidad de la Enfermedad , Resultado del Tratamiento , Escala Visual Analógica
17.
Chin Med J (Engl) ; 130(21): 2535-2540, 2017 Nov 05.
Artículo en Inglés | MEDLINE | ID: mdl-29067951

RESUMEN

BACKGROUND: Thoracolumbar junction (TLJ) is the transitional area between the lower thoracic spine and the upper lumbar spine. Vertebral compression fractures and proximal junctional kyphosis following spine surgery often occur in this area. Therefore, the study of development and mechanisms of thoracolumbar junctional degeneration is important for planning surgical management. This study aimed to review radiological parameters of thoracolumbar junctional degenerative kyphosis (TLJDK) in patients with lumbar degenerative kyphosis and to analyze compensatory mechanisms of sagittal balance. METHODS: From January 2016 to March 2017, patients with lumbar degenerative kyphosis were enrolled in this radiographic study. Patients were divided into two groups according to thoracolumbar junctional angle (TLJA): the non-TLJDK (NTLJDK) group (TLJA <10°) and the TLJDK group (TLJA ≥10°). Complete spinopelvic radiographic parameters were analyzed and compared between two groups. Pearson or Spearman correlation coefficients and independent two-sample t-test or Mann-Whitney U-test were used. RESULTS: A total of 77 patients with symptomatic sagittal imbalance due to lumbar degenerative kyphosis were enrolled in this study. There were 34 patients in NTLJDK group (TLJA <10°) and 43 patients in TLJDK group (TLJA ≥10°). The median angle of lumbar lordosis (LL) in the NTLJDK or TLJDK groups was 23.40° (18.50°, 29.48°) or 19.50° (13.30°, 24.55°), respectively. The median TLJAs in all patients and both groups were -11.20° (-14.60°, -4.80°), -3.70° (-7.53°, -1.73°), and -14.30° (-17.45°, -13.00°), respectively. In the NTLJDK group, LL was correlated with thoracic kyphosis (TK; r = -0.400, P = 0.019), sacral slope (SS; r = 0.681, P < 0.001), and C7-sagittal vertical axis (r = -0.402, P = 0.018). In the TLJDK group, LL was correlated with TK (r = -0.345, P = 0.024), SS (r = 0.595, P < 0.001), and pelvic tilt (r = -0.363, P = 0.017). There were significant differences in LL, TLJA, TK, SS, and pelvic incidence (PI) between two groups. CONCLUSIONS: Although TLJDK is common in patients with lumbar degenerative kyphosis, it might be generated by special characteristics of morphology and biomechanics of the TLJ. To maintain sagittal balance, pelvis back tilt might be more important in patients with TLJDK, whereas thoracic curve changes might be more important in patients without TLJDK.


Asunto(s)
Cifosis/diagnóstico por imagen , Fracturas de la Columna Vertebral/diagnóstico por imagen , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Cifosis/patología , Lordosis/diagnóstico por imagen , Lordosis/patología , Vértebras Lumbares/diagnóstico por imagen , Vértebras Lumbares/patología , Masculino , Persona de Mediana Edad , Escoliosis/diagnóstico por imagen , Escoliosis/patología , Fracturas de la Columna Vertebral/patología , Vértebras Torácicas/diagnóstico por imagen , Vértebras Torácicas/patología
18.
Oncotarget ; 7(48): 78985-78993, 2016 Nov 29.
Artículo en Inglés | MEDLINE | ID: mdl-27738317

RESUMEN

OBJECTIVES: Estimate the epidermal growth factor receptor (EGFR) mutation prevalence in all non-small cell lung cancer (NSCLC) patients and patient subgroups. RESULTS: A total of 456 studies were included, reporting 30,466 patients with EGFR mutation among 115,815 NSCLC patients. The overall pooled prevalence for EGFR mutations was 32.3% (95% CI 30.9% to 33.7%), ranging from 38.4% (95% CI: 36.5% to 40.3%) in China to 14.1% (95% CI: 12.7% to 15.5%) in Europe. The pooled prevalence of EGFR mutation was higher in females (females vs. males: 43.7% vs. 24.0%; OR: 2.7, 95% CI: 2.5 to 2.9), non-smokers (non-smokers vs. past or current smokers: 49.3% vs. 21.5%; OR: 3.7, 95% CI: 3.4 to 4.0), and patients with adenocarcinoma (adenocarcinoma vs. non-adenocarcinoma: 38.0% vs. 11.7%; OR: 4.1, 95% CI: 3.6 to 4.8). MATERIALS AND METHODS: PubMed, EMBASE, and the Cochrane Library were searched to June 2013. Eligible studies reported EGFR mutation prevalence and the association with at least one of the following factors: gender, smoking status and histology. Random-effects models were used to pool EGFR mutation prevalence data. CONCLUSION: This study provides the exact prevalence of EGFR mutations in different countries and NSCLC patient subgroups.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/genética , Receptores ErbB/genética , Neoplasias Pulmonares/genética , Mutación , Pueblo Asiatico/genética , China , Europa (Continente) , Femenino , Predisposición Genética a la Enfermedad , Humanos , Masculino , Tasa de Mutación , Prevalencia , Factores de Riesgo , Fumar/genética , Población Blanca/genética
19.
Biomed Environ Sci ; 27(5): 391-5, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-24827722

RESUMEN

The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.


Asunto(s)
Fibronectinas/efectos de la radiación , Prótesis e Implantes/microbiología , Esterilización/métodos , Adhesión Celular/efectos de la radiación , Filtración , Rayos Ultravioleta
20.
Huan Jing Ke Xue ; 35(10): 3794-9, 2014 Oct.
Artículo en Chino | MEDLINE | ID: mdl-25693385

RESUMEN

This paper studied the phtodegradation behavior, mechanism and security of propranolol (PRO) in water under ultraviolet irradiation using the high pressure mercury lamp. The photodegradation mechanism was verified by reactive oxygen species (ROS) quenching method, and the photoproducts security was evaluated by luminescent bacteria toxicity test. The results showed that the rate of photolysis (k) of PRO decreased with increasing initial concentration, and showed a significant negative correlation (r2 > 0.95). The increasing k value was also accompanied with higher pH of the solution, and the k values were 0.0953-0.267 min(-1) under pH 5-9. ROS quenching experiments showed that the PRO UV photolysis process included a triplet PRO (3PRO*) direct photolysis participation, and self-sensitized photolysis participation caused by hydroxyl radical (·OH) and singlet oxygen (1O2). Direct photolysis rate was greater than the rate of self-sensitized photolysis. The result of 1O2 steady concentration measured by FFA as the probe was consistent with the quenching method. The toxicity evaluation illustrated the formation of some intermediate photoproducts, which were more toxic than PRO.


Asunto(s)
Fotólisis , Propranolol/química , Rayos Ultravioleta , Bacterias , Radical Hidroxilo/química , Mediciones Luminiscentes , Propranolol/efectos de la radiación , Oxígeno Singlete/química , Soluciones , Pruebas de Toxicidad , Agua
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