Multicomponent Synergistic Antibacterial Hydrogel Based on Gelatin-Oxidized Carboxymethyl Cellulose for Wound Healing of Drug-Resistant Chronic Infection.

Bacterial invasion hinders the healing process of wound, leading to the formation of chronic infected wound; meanwhile, the misuse of antibiotics has resulted in the emergence of numerous drug-resistant bacteria. The application of conventional antimicrobial methods and wound treatment techniques is not appropriate for wound dressings. In this paper, quaternized poly(vinyl alcohol) (QPVA) and pomegranate-like copper uniformly doped polydopamine nanoparticles (PDA@Cu) were introduced into a gelatin-oxidized carboxymethyl cellulose system to form a multicomponent synergistic antibacterial hydrogel (GOQ3P3). Polydopamine improves the biocompatibility and prevents the detachment of Cu nanoparticles. It can achieve synergistic antibacterial effects through quaternary ammonium salt-inorganic nanoparticle photothermal treatment under 808 nm near-infrared (NIR) irradiation. It exhibits highly efficient and rapid bactericidal properties against Escherichia coli, Staphylococcus aureus, and MRSA (methicillin-resistant Staphylococcus aureus) with an antibacterial rate close to 100%. The gel scaffold composed of macromolecules gives the hydrogel excellent mechanical properties, adhesive capabilities, self-healing characteristics, biocompatibility, and pH degradation and promotes cell adhesion and migration. In a full-thickness wound healing model infected with MRSA, GOQ3P3 controls inflammatory responses, accelerates collagen deposition, promotes angiogenesis, and enhances wound closure in the wound healing cascade reaction. This study provides a feasible strategy for constructing dressings targeting chronic infection wounds caused by drug-resistant bacteria.

The synergetic effect of alginate-derived hydrogels and metal-phenolic nanospheres for chronic wound therapy.

Management of diabetic wounds presents a global health challenge due to elevated levels of ROS in the wound microenvironment, persistent dysregulation of inflammation modulation, and limitations in commercially available dressings. Addressing this issue, we have developed a pH-responsive and glucose-sensitive multifunctional hydrogel dressing that dynamically responds to the wound microenvironment and enables on-demand drug release. The dressing incorporates a matrix material based on aminophenylboronic acid-functionalized alginate and a polyhydroxy polymer, alongside an enhancer phase consisting of self-assembled metal-phenol coordination nanospheres formed by tannic acid and iron ions. Using the dynamic borate ester bonds and catechol-metal ion coordination bonds, the dressing exhibits remarkable shape adaptability, self-healing capability, tissue adhesiveness, antioxidant activity, and photothermal responsiveness, without additional curatives or crosslinking agents. As a wound dressing, it elicits macrophage polarization towards an anti-inflammatory phenotype while maintaining long-lasting antimicrobial effects. In a diabetic mouse model of full-thickness wound infections, it effectively mitigated inflammation and vascular damage, significantly expediting the wound healing process with a commendable 97.7% wound closure rate. This work provides a new direction for developing multifunctional smart hydrogel dressings that can accelerate diabetic wound healing for human health.

Dehydration-Toughing Dual-Solvent Gels with Viscoelastic Transition for Infectious Wound Treatment.

The modulus of traditional biomedical hydrogels increases exponentially meditated by dehydration-stiffing mechanism, which leads to the failure of interface matching between hydrogels and soft tissue wounds. It is found in the study that the dual-solvent gels exhibit dehydration-toughening mechanism with the slowly increasing modulus that are always match the soft tissue wounds. Therefore, dual-solvent glycerol hydrogels (GCFen-gly DGHs) are prepared with hydrophobically modified catechol chitosan (hmCSC) and gelatin based on the supramolecular interactions. GCFen-gly DGHs exhibit excellent water retention capacity with a total solvent content exceeding 80%, permanent skin-like modulus within a range of 0.45 to 4.13 kPa, and stable photothermal antibacterial abilities against S, aureus, E. coli, as well as MRSA. Infectious full-thickness rat skin defect model and tissue section analysis indicate that GCFen-gly DGHs are able to accelerate infectious wound healing by alleviating the inflammatory response, promoting granulation tissue growth, re-epithelialization, collagen deposition, and vascular regeneration. As a result, GCFen-gly DGHs is expected to become the next-generation biological gel materials for infectious wound treatment.

Dual-Drug-Loaded Core-Shell Electrospun Nanofiber Dressing for Deep Burns.

The epidermis of a deep burn wound is entirely absent and the dermal tissue sustains significant damage, accompanied by a substantial amount of tissue exudate. Due to the excessively humid environment, the formation of a scab on the wound becomes challenging, leaving it highly vulnerable to external bacterial invasion. In this work, a core-shell dual-drug-loaded nanofiber dressing was prepared by electrospinning technology for the synergistic treatment of a deep burn. The shell layer consists of polycaprolactone and chitosan encapsulating asiaticoside, with the core layer comprising the clathrate of 2-hydroxypropyl-ß-cyclodextrin and curcumin. Upon application to the wound, the dual-drug-loaded nanofiber dressing exhibited rapid release of asiaticoside, stimulating collagen deposition and promoting tissue repair. The core-shell structure and clathrate configuration ensured sustained release of curcumin, providing antibacterial and anti-inflammatory functions for the wound. The mechanical strength, broad-spectrum antibacterial ability, cell proliferation, and adhesion ability of the nanofiber dressing showed its potential as a medical dressing. This dressing also exhibited excellent wound healing promoting effects in the SD rat burn model. This paper provides a strategy for burn wound healing.

Identification of lncRNAs associated with uterine corpus endometrial cancer prognosis based on the competing endogenous RNA network.

Uterine Corpus Endometrial Carcinoma (UCEC) is one of the major malignant tumors of the female reproductive system. However, there are limitations in the currently available diagnostic approaches for UCEC. Long non-coding RNAs (lncRNAs) play important roles in regulating biological processes as competitive endogenous RNA (ceRNA) in tumors. To study the potential of lncRNAs as non-invasive diagnostic tumor markers, RNA-sequencing dataset of UCEC patients from The Cancer Genome Atlas was used to identify differentially expressed genes. A lncRNA-miRNA-mRNA ceRNA network was constructed by differentially expressed lncRNAs, miRNAs and miRNAs. Pathway enrichment and functional analysis for the mRNAs in the constructed ceRNA network provide the direction of future research for UCEC by demonstrating the most affected processes and pathways. Seven potential lncRNA biomarkers (C20orf56, LOC100144604, LOC100190940, LOC151534, LOC727677, FLJ35390, LOC158572) were validated in UCEC patients by quantitative real-time PCR. Notably, LOC100190940 and LOC158572 were identified as novel RNA molecules with unknown functions. Receiver operating characteristic (ROC) curve analysis demonstrated that the combined 7 lncRNAs had a high diagnostic value for UCEC patients with area under curve (AUC) of 0.941 (95% CI: 0.875-0.947). Our study highlights the potential of the validated 7 lncRNAs panel as diagnostic biomarkers in UCEC, providing new insights into the UCEC pathogenesis.

A Novel Strategy for the Synthesis of High Stability of Luminescent Zero Dimensional-Two Dimensional CsPbBr3 Quantum Dot/1,4-bis(4-methylstyryl)benzene Nanoplate Heterostructures at an Atmospheric Condition.

Perovskite quantum dots (QDs), emerging with excellent bright-green photoluminescence (PL) and a large absorption coefficient, are of great potential for the fabrication of light sources in underwater optical wireless communication systems. However, the instability caused by low formation energy and abundant surface traps is still a major concern for perovskite-based light sources in underwater conditions. Herein, we propose ultra-stable zero dimensional-two dimensional (0D-2D) CsPbBr3 QD/1,4-bis(4-methylstyryl)benzene (p-MSB) nanoplate (NP) heterostructures synthesized via a facile approach at room temperature in air. CsPbBr3 QDs can naturally nucleate on the p-MSB NP toluene solution, and the radiative combination is drastically intensified owing to the electron transfer within the typical type-II heterostructures, leading to a sharply increased PLQY of the heterostructure thin films up to 200% compared with the pristine sample. The passivation of defects within CsPbBr3 QDs can be effectively realized with the existence of p-MSB NPs, and thus the obviously improved PL is steadily witnessed in an ambient atmosphere and thermal environment. Meanwhile, the enhanced humidity stability and a peak EQE of 9.67% suggests a synergetic strategy for concurrently addressing the knotty problems on unsatisfied luminous efficiency and stability of perovskites for high-performance green-emitting optoelectronic devices in underwater applications.

Diagnostic value of shear wave elastography combined with super microvascular imaging for BI-RADS 3-5 nodules.

Background: To investigate the diagnostic value of shear wave elastography (SWE) and super microvascular imaging (SMI) integrated with the traditional ultrasound breast imaging reporting and data system (BI-RADS) classification in differentiating between benign and malignant breast nodules. Methods: For analysis, 88 patients with 110 breast nodules assessed as BI-RADS 3-5 by conventional ultrasound were selected. SWE and SMI evaluations were conducted separately, and all nodules were verified as benign or malignant ones by pathology. Receiver operating characteristic (ROC) curves were plotted after obtaining quantitative parameters of different shear waves of nodules, including maximum (Emax), mean (Emean), minimum (Emin) Young's modulus, modulus standard deviation (SD), and modulus ratio (Eratio). The best cut-off value, specificity, sensitivity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) for diagnosing malignant nodules employing Emax were obtained, and the diagnostic value of combining Emax and BI-RADS classification was compared. SMI graded nodule based on the Alder blood flow grading standard, whereas the BI-RADS classification was based on microvascular morphology. We assessed the diagnostic value of SMI for breast nodules and investigated the diagnostic efficacy of SWE combined with SMI in differentiating benign and malignant breast nodules with BI-RADS classification 3-5. Results: The adjusted the BI-RADS classification using SMI and SWE technologies promoted the sensitivity, specificity, and accuracy of discriminating benign and malignant breast nodules (P < 0.05). The combination of traditional ultrasound BI-RADS classification with SWE and SMI technologies offered high sensitivity, specificity, accuracy, PPV, and NPV for identifying benign and malignant breast lesions. Moreover, combining SWE and SMI technologies with the adjusted BI-RADS classificationhad the best diagnostic efficacy for distinguishing benign and malignant breast nodules with BI-RADS 3-5. Conclusion: The combination of SWE and SMI with the adjusted BI-RADS classification is a promising diagnostic method for differentiating benign and malignant breast nodules.

Poly(p-phenylenediamine)-Coated Metal-Organic Frameworks for High-Performance Sodium-Ion Batteries: The Balance of Capacity and Stability.

Metal-organic frameworks (MOFs) with well-defined porous structures and highly active frameworks are considered as promising electrode materials for sodium-ion batteries (SIBs). However, the structure pulverization upon sodiation/desodiation impacts on their practical application in SIBs. To address this issue, poly(p-phenylenediamine) (PPA) was uniformly coated onto the surface of MIL-88A, a typical Fe-based MOF through in situ polymerization initiated by the metal ions (Fe3+) of MIL-88A. Used as an anode material for SIBs, the PPA-coated MIL-88A, denoted as PPA@MIL-88A, showed significantly improved electrochemical performance. A reversible capacity as high as 230 mAh g-1 was achieved at 0.2 A g-1 even after 500 cycles. MIL-88A constructed with electrochemically active Fe3+ and fumaric acid ligands guarantees the high specific capacity, while the PPA polymer coating effectively inhibits the pulverization of MIL-88A. This work provides an efficient strategy for improving the structure and cycling stability of MOFs-based electrode materials.

A biomimetic piezoelectric scaffold with sustained Mg2+ release promotes neurogenic and angiogenic differentiation for enhanced bone regeneration.

Natural bone is a composite tissue made of organic and inorganic components, showing piezoelectricity. Whitlockite (WH), which is a natural magnesium-containing calcium phosphate, has attracted great attention in bone formation recently due to its unique piezoelectric property after sintering treatment and sustained release of magnesium ion (Mg2+). Herein, a composite scaffold (denoted as PWH scaffold) composed of piezoelectric WH (PWH) and poly(ε-caprolactone) (PCL) was 3D printed to meet the physiological demands for the regeneration of neuro-vascularized bone tissue, namely, providing endogenous electric field at the defect site. The sustained release of Mg2+ from the PWH scaffold, displaying multiple biological activities, and thus exhibits a strong synergistic effect with the piezoelectricity on inhibiting osteoclast activation, promoting the neurogenic, angiogenic, and osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs) in vitro. In a rat calvarial defect model, this PWH scaffold is remarkably conducive to efficient neo-bone formation with rich neurogenic and angiogenic expressions. Overall, this study presents the first example of biomimetic piezoelectric scaffold with sustained Mg2+ release for promoting the regeneration of neuro-vascularized bone tissue in vivo, which offers new insights for regenerative medicine.

Impact of smart health systems on the behavior of older adults under community healthcare.

Background: With the trend of world population aging, a good community health care system will determine whether the elderly can get good medical conditions. How to improve the community health care system can study how the behavior of the elderly affects it. Objective: This paper is based on the analysis of the current situation of population aging at home and abroad. Methods: On the premise of analyzing the demand and behavior of elderly people seeking medical treatment and the function of community health service institutions. Literature research was conducted to analyze the influencing factors of community health care needs and elderly people's medical seeking behavior at home and abroad. Then the elderly in Tianjin were investigated by issuing questionnaires, and the law of medical treatment behavior of the elderly in Tianjin was calculated. Combined with the results of relevant investigations abroad, the common phenomenon is summarized. Finally, the analysis method of intelligent medical system is proposed, and the design process of system acquisition module and user usage mode are given. Result: The smart medical system can bring great convenience to the elderly and community healthcare. Discussion: It emphasizes the powerful functions of smart health systems and their future importance for the health care of the elderly.

Dilated transformer: residual axial attention for breast ultrasound image segmentation.

Background: The segmentation of breast ultrasound (US) images has been a challenging task, mainly due to limited data and the inherent image characteristics involved, such as low contrast and speckle noise. Although convolutional neural network-based (CNN-based) methods have made significant progress over the past decade, they lack the ability to model long-range interactions. Recently, the transformer method has been successfully applied to the tasks of computer vision. It has a strong ability to capture distant interactions. However, most transformer-based methods with excellent performance rely on pre-training on large datasets, making it infeasible to directly apply them to medical images analysis, especially that of breast US images with limited high-quality labels. Therefore, it is of great significance to find a robust and efficient transformer-based method for use on small breast US image datasets. Methods: We developed a dilated transformer (DT) method which mainly uses the proposed residual axial attention layers to build encoder blocks and the introduced dilation module (DM) to further increase the receptive field. We evaluated the proposed method on 2 breast US image datasets using the 5-fold cross-validation method. Dataset A was a public dataset with 562 images, while dataset B was a private dataset with 878 images. Ground truth (GT) was delineated by 2 radiologists with more than 5 years of experience. The evaluation was followed by related ablation experiments. Results: The DT was found to be comparable with the state-of-the-art (SOTA) CNN-based method and outperformed the related transformer-based method, medical transformer (MT), on both datasets. Especially on dataset B, the DT outperformed the MT on metrics of Jaccard index (JI) and Dice similarity coefficient (DSC) by 2.67% and 4.68%, respectively. Meanwhile, when compared with Unet, the DT improved JI and DSC by 4.89% and 4.66%, respectively. Moreover, the results of the ablation experiments showed that each add-on part of the DT is important and contributes to the segmentation accuracy. Conclusions: The proposed transformer-based method could achieve advanced segmentation performance on different small breast US image datasets without pretraining.

Accurate segmentation of breast tumor in ultrasound images through joint training and refined segmentation.

Objective.This paper proposes an automatic breast tumor segmentation method for two-dimensional (2D) ultrasound images, which is significantly more accurate, robust, and adaptable than common deep learning models on small datasets.Approach.A generalized joint training and refined segmentation framework (JR) was established, involving a joint training module (Jmodule) and a refined segmentation module (Rmodule). InJmodule, two segmentation networks are trained simultaneously, under the guidance of the proposed Jocor for Segmentation (JFS) algorithm. InRmodule, the output ofJmoduleis refined by the proposed area first (AF) algorithm, and marked watershed (MW) algorithm. The AF mainly reduces false positives, which arise easily from the inherent features of breast ultrasound images, in the light of the area, distance, average radical derivative (ARD) and radical gradient index (RGI) of candidate contours. Meanwhile, the MW avoids over-segmentation, and refines segmentation results. To verify its performance, the JR framework was evaluated on three breast ultrasound image datasets. Image dataset A contains 1036 images from local hospitals. Image datasets B and C are two public datasets, containing 562 images and 163 images, respectively. The evaluation was followed by related ablation experiments.Main results.The JR outperformed the other state-of-the-art (SOTA) methods on the three image datasets, especially on image dataset B. Compared with the SOTA methods, the JR improved true positive ratio (TPR) and Jaccard index (JI) by 1.5% and 3.2%, respectively, and reduces (false positive ratio) FPR by 3.7% on image dataset B. The results of the ablation experiments show that each component of the JR matters, and contributes to the segmentation accuracy, particularly in the reduction of false positives.Significance.This study successfully combines traditional segmentation methods with deep learning models. The proposed method can segment small-scale breast ultrasound image datasets efficiently and effectively, with excellent generalization performance.

A Review: Current Status and Emerging Developments on Natural Polymer-Based Electrospun Fibers.

In recent years, natural polymer-based electrospun fibers (EFs) with huge specific surface area, good biocompatibility, and biological activity obtained from electrospinning process exhibit tremendous vitality in the field of biomedical areas. Herein, the parameters of electrospinning from two perspectives, polymer solution such as solvent, polymeric relative molecular mass, concentration, viscosity, and conductivity of the solution, and electrospinning process such as spinning voltage, spinning flow rate, needle tip to collector distance, temperature, and humidity are first detailed. Next, the raw materials consisting of polysaccharides such as cellulose, hyaluronic acid, alginate, and chitosan as well as proteins such as collagen, gelatin, silk fibroin, and keratin are summarized. The preparation method and related characteristics of EFs with multistage structures such as porous, core-shell, Janus, bamboo-like and other structures are introduced. The biomedical applications of these natural polymer-based EFs mainly including tissue engineering, drug sustained release, wound dressings, and biomedical sensors are systematically recapitulated. Finally, the outlook on natural EFs is further proposed.

Clinical Manifestations of Supra-Large Range Nonperfusion Area in Diabetic Retinopathy.

Objective: We describe the clinical manifestations of supra-large range nonperfusion area (SLRNPA) in diabetic retinopathy (DR). Methods: This was a retrospective case-control study. A total of 260 eyes of 236 patients with DR who underwent pars plana vitrectomy in the Department of Ophthalmology of Qingdao Municipal Hospital from February 2016 to June 2019 were enrolled. Fundus fluorescein angiography was performed after surgery to determine whether SLRNPA or non-SLRNPA in DR was present. All demographic and clinical data were carefully collected. Results: Forty-one eyes of 22 patients were diagnosed with SLRNPA in DR (15.77% of all eyes). Compared to non-SLRNPA, SLRNPA patients were more likely to be male and younger with earlier DR onset, a smoking history, other comorbidities, and a higher HbA1c level. SLRNPA in DR eyes exhibited more neovascular glaucoma (NVG) and diabetic keratopathy (DK) than did other eyes. Such eyes were more likely to require anti-VEGF therapy before surgery or a silicone oil or a gas tamponade during surgery and to suffer from persistent corneal epithelial erosion and NVG recurrence after surgery. Conclusions: SLRNPA in DR is a severe status of DR. Treatment for DR patients with SLRNPA is difficult, and the prognosis is poor, so clinicians must thus pay more attention to SLRNPA in DR.

Targeting polysaccharides such as chitosan, cellulose, alginate and starch for designing hemostatic dressings.

Unfortunately hemorrhage and its complications (e.g. anemia, organ failure, and hypothermia) induced by traumatic injury, surgery, and disorders of bleeding play an all too familiar role in human morbidity and mortality. Therefore, it is difficult to overstate the importance of better understanding the role of polysaccharides in advanced hemostatic dressings (HDs). This review includes consideration of polysaccharide hemostatic dressing mechanism of action, relative efficacy, cost and safety. Polysaccharide-based HDs are widely used in management not only of external and internal bleeding but also of massive hemorrhage. These polysaccharide-based HDs have been shown to be effective in both compressible and non-compressible hemorrhage. Hemostatic dressings are designed with different principles depending on location and extent of injury. This review focuses on polysaccharide HD design and associated hemostatic mechanisms. It addresses current issues, challenges, and future perspectives.

Smart Polycationic Hydrogel Dressing for Dynamic Wound Healing.

It is challenging for traditional wound dressings to adapt to the complex and changeable environment, due to the lack of stable, efficient, and continuous bactericidal activity. They also cannot be satisfied in a multifunctional sensing platform to reconstruct skin sensory functions for human health monitoring. A multifunctional hydrogel dressing is developed here for the treatment of infected wounds and human health monitoring, which is based on alginate and polycation. The in situ polymerization and solvent displacement method are used to functionalize the hydrogel for the improvement of antifreezing, water retention, and environmental adaptability, as well as the adhesion and photothermal property. As a wound dressing, the as-prepared hydrogel exhibits an excellent antibacterial property against both Escherichia coli and Staphylococcus aureus. In a rat model of full-thickness wound infection, it significantly accelerates the healing of infected wounds with a high healing rate of 96.49%. In the further multifunctional sensory tests, the hydrogel shows multiple response modes of strain, pressure and temperature, and sensing stability. An idea is provided here to develop a smart hydrogel dressing that can accelerate wound healing and achieve human health monitoring.

Intraocular myofibroblastoma tumour of the ciliary body: a case report and literature review.

BACKGROUND: Inflammatory Myofibroblastoma Tumors (IMTs) are extremely tumour rare in the intraocular. CASE PRESENTATION: A ciliary body tumor was found under slit lamp biomicroscopy in a 55-year-old male first diagnosed with cataract. Then this patient underwent trans-sclera resection via partial lamellar sclerouvectomy and par plans vitrectomy to remove the mass. Hematoxylin and eosin (HE) staining and immunohistochemistry findings showed that the characteristics of the tumor were consistent with IMT. CONCLUSIONS: We reported a rare case of intraocular IMT, which is confirmed by H&E staining, and IHC positive staining for Vimentin, Desmin and ALK, while negative staining for SMA, S-100, ki-67, CK, CD68, and calponin.

Highly Reversible Zinc Anode Enabled by a Cation-Exchange Coating with Zn-Ion Selective Channels.

Rechargeable aqueous zinc-ion batteries (ZIBs) have attracted extensive attention due to their low cost and high safety. However, the critical issues of dendrite growth and side reactions on the Zn metal anode hinder the commercialization of ZIBs. Herein, we demonstrated that the formation of Zn4SO4(OH)6·5H2O byproducts is closely relevant to the direct contact between the Zn electrode and SO42-/H2O. On the basis of this finding, we developed a cation-exchange membrane of perfluorosulfonic acid (PFSA) coated on the Zn surface to regulate the Zn plating/stripping behavior. Importantly, the PFSA film with abundant sulfonic acid groups could simultaneously block the access of SO42- and H2O, accelerate the Zn2+ ion transport kinetics, and uniformize the electrical and Zn2+ ion concentration field on the Zn surface, thus achieving a highly reversible Zn plating/stripping process with corrosion-free and dendrite-free behavior. Consequently, the PFSA-modified Zn anode exhibits high reversibility with 99.5% Coulombic efficiency and excellent plating/stripping stability (over 1500 h), subsequently enabling a highly rechargeable Zn-MnO2 full cell. The strategy of the cation-exchange membrane proposed in this work provides a simple but efficient method for suppression of side reactions.

Smart Hydrogel Sensors with Antifreezing, Antifouling Properties for Wound Healing.

Flexible electronic devices with biological therapeutic and sensing properties are one of the current research directions. Here, a multifunctional hydrogel for stress sensing and wound healing was prepared by a simple one-pot method and a solution replacement method. Among them, zwitterionic polymers promote wound healing by promoting the polarization of M2 macrophages, collagen deposition, and blood vessel formation. Glycerin can significantly improve the resilience and frost resistance of the hydrogel, ensuring that a sensor made using the hydrogel can work normally in a cold environment. In addition, zwitterionic polymers are highly biocompatible, providing excellent antibacterial adhesion to aid the wound healing process, and good electrical conductivity enhances sensing sensitivity and stability. Based on these properties, multifunctional hydrogels could detect human vital activities while promoting wound healing, providing new ideas for the fields of diagnosis and wound dressing.

Chitosan for constructing stable polymer-inorganic suspensions and multifunctional membranes for wound healing.

Restricted by the rigid chain structure, chitosan-based materials are very fragile and easy to break up. Herein, a stable and positively charged chitosan/Ti3C2Tx suspension was successfully constructed by adjusting mixing sequence, pH and ratio of chitosan to Ti3C2Tx to prepare a multifunctional membrane (P-CM) with self-standing ability, good flexibility, biocompatibility, excellent photothermal antibacterial properties, and sensitive humidity sensing properties. In the first stage, protonated chitosan acted as sugar coatings was able to be encapsulated on the surface of Ti3C2Tx nanosheets by electrostatic self-assembly, in turn achieving charge reversal of the nanoparticles. In the second stage, chitosan acted as molecular needles to suture the membrane formed by chitosan/Ti3C2Tx particles via vacuum filtration (VAF) with the commercial flexible poly(vinylidene fluoride) (PVDF) membrane. The as-prepared P-CM membrane simultaneously solves the problem of insufficient flexibility of a single component chitosan membrane and the poor bonding between pure Ti3C2Tx membranes and substrate membrane.