Chrysanthemum morifolium attenuates metabolic and alcohol-associated liver disease via gut microbiota and PPARα/γ activation.

BACKGROUND: Metabolic and alcohol-associated liver disease (MetALD) shows a high prevalence rate in liver patients, but there is currently no effective treatment for MetALD. As a typical edible traditional Chinese medicinal herb, the anti-inflammatory, antioxidant, and hepatoprotective properties of water extract of Chrysanthemum morifolium Ramat. (WECM) has been demonstrated. However, its therapeutic effect on MetALD and the associated mechanisms remain unclear. PURPOSE: To investigate the underlying mechanisms of WECM against MetALD. METHODS: We constructed a MetALD rat model following a high-fat & high-sucrose plus alcohol diet (HFHSAD). MetALD rats were treated with WECM at 2.1, 4.2, and 8.4 g/kg/d for six weeks. Efficacy was determined, and pathways associated with WECM against MetALD were predicted through serum and hepatic biochemical marker measurement, histopathological section analysis, 16S rDNA sequencing of the gut microbiota and untargeted serum metabolomics analyses. Changes in genes and proteins in the peroxisome proliferator-activated receptor alpha (PPARα) and gamma (PPARγ) signaling pathways were detected by RT‒PCR and Western blotting. RESULTS: WECM treatment significantly attenuated hepatic steatosis, hyperlipidemia and markers of liver injury in MetALD rats. Moreover, WECM improved vascular endothelial function, hypertension, and systematic oxidative stress. Mechanistically, WECM treatment altered the overall structure of the gut microbiota through maintaining Firmicutes/Bacteroidota ratio and reducing harmful bacterial abundances such as Clostridium, Faecalibaculum, and Herminiimonas. Notably, WECM promoted 15-deoxy-△12, 14-prostaglandin J2 (15d-PGJ2) release and further activated the PPARγ to reduce serum TNF-α, IL-1ß, and IL-6 levels. Additionally, WECM upregulated PPARα and downregulated the levels of CD36 and FABP4 to improve lipid metabolism. CONCLUSION: Our findings provide the first evidence that WECM treatment significantly improved hepatic steatosis, oxidative stress and inflammation in MetALD rats by regulating the gut microbiota and activating the 15d-PGJ2/PPARγ and PPARα signaling pathway.

Simulation of spatial and temporal variation of nitrate leaching in the vadose zone of alluvial regions on a large regional scale.

Excessive use of fertilizers presents a significant threat to groundwater safety. To mitigate nitrate leaching and ensure the sustainable utilization of groundwater resources, it is crucial to quantify the spatial heterogeneity of nitrogen leaching and its drivers. Therefore, accurate modeling of deep nitrate leaching at large regional scales is necessary. In this study, we have created a computational framework to analyze the transport of unsaturated zone water and nitrate at a regional scale. The framework is based on a process-oriented, watershed-scale computational model that segments the study area into a grid system, with each grid modeled using Richards-based advection-diffusion equations for water and solutes. The research model estimated nitrate nitrogen leaching, accumulation, and denitrification in the vadose zone of agricultural fields in the Baiyangdian watershed, which is a typical agricultural region with complex land use and soil deposition conditions in the North China Plain. The results showed that there were significant spatial differences in nitrate N leaching, denitrification and accumulation with values of 0-388 kg/ha/year, 30-177 kg/ha/year and 75-4778 kg/ha. Groundwater recharge in the wheat/maize, vegetable, and cotton area exhibited a negative correlation with nitrate N accumulation while showing a positive correlation with nitrate N leaching. Nitrate nitrogen distribution indicated spatial heterogeneity, attributable mainly to the heterogeneity in soil texture, structure, and land use. With nitrate nitrogen leaching and denitrification levels reaching 327-388 kg/ha/year and 133-175 kg/ha/year, respectively, vegetable fields pose a direct threat to groundwater. Meanwhile, wheat/maize fields showed the greatest nitrate nitrogen accumulation, ranging from 624 to 4778 kg/ha. This excessive buildup of nitrate in these fields presents a potential hazard to groundwater quality. Soil texture in the root zone had a greater influence on the amount of nitrate leaching and denitrification than soil texture below the root zone. Deeper soil texture (>2 m) was found to mainly control total nitrate accumulation in the vadose zone. To assess nitrate leaching, denitrification, and accumulation at a regional scale within the deep vadose zone, a process-oriented model was developed, considering the intricate associations among land usage, soil texture, and biochemical reactions.

Comparison of Pivot Profile (PP), Rate-All-That-Apply (RATA), and Pivot-CATA for the sensory profiling of commercial Chinese tea products.

Rapid sensory profiling methods relying on consumers' perceptions are getting prevalent and broadly utilized by labs and companies to supersede conventional sensory profiling methodologies. Till now, various intensity-based sensory methods such as the newly proposed Pivot-Check-All-That-Apply (CATA) are limitedly developed and compared. In this investigation, Pivot Profile (PP), Rate-All-That-Apply (RATA), and Pivot-CATA methods were applied and validated using tea consumers and commercial Chinese tea products as samples. Data from three approaches were collected, analyzed by correspondence analysis (CA), and used to compare the three methods assessing the panel assessment process, sensory maps, confidence ellipses, and practical applications. Pivot-CATA exhibited a high similarity with RATA (RV = 0.873), and a lower similarity with PP (RV = 0.629). Of the three intensity-related methods, confidence ellipses on the RATA sensory map were the smallest and overlapped the least. However, Pivot-CATA consumed less time in collecting data and its questionnaire was more friendly to participants compared with PP and made the difference in intensity of samples more noticeable to the participants than RATA due to the existence of the pivot sample. Its experimental versatility also allows for a wide range of applications, indicating that the Pivot-CATA is an approach with great promise for routine use.

Enhanced dewaterability of sewage sludge by grafted cationic lignin-based flocculants.

Lignin-based flocculants are widely used for wastewater purification, but their application in sludge dewatering has not yet been documented. In this study, a novel cationic lignin-based flocculant named LS-g-CPA was prepared by grafting cationic polyacrylamide (CPA) synthesized from methacryloyloxy ethyltrimethyl ammonium chloride (DMC) and acrylamide (AM) onto sodium lignosulfonate (LS), and its roles and underlying mechanisms in sludge conditioning were investigated. The results showed that LS-g-CPA effectively improved the dewaterability of sludge, reducing the filtration resistance and filter cake moisture content of sludge from 0.61 ± 0.05 × 1012 m/kg to 0.14 ± 0.02 × 1012 m/kg and 85.64 ± 0.25 % to 76.84 ± 0.41 %, respectively. The dewatering performance of LS-g-CPA was positively correlated with the DMC/AM ratio. The quaternary ammonium groups brought by DMC disrupted the reticular structure of extracellular polymeric substances, exposing hydrophobic residues and releasing bound water. Nevertheless, the key to LS-g-CPA for improving sludge dewatering lies more in the amphoteric flocculant properties that enhance sludge flocculation and the octopus-type structure that provides good drainage channels. This study reveals that lignin-based flocculants are effective in improving the dewaterability of sludge, which provides direct evidence for their application in sludge dewatering.

Er: YAG laser assisted photodynamic therapy for the management of severe oral epithelial dysplasia with innate and adaptive immune responses.

Oral epithelial dysplasia (OED) in oral potentially malignant disorders (OPMDs) increases the risk of malignant transformation. However, the management of OED is not well defined. Photodynamic therapy (PDT) is a hypotoxic, highly selective and minimally-invasive operation which reduces morbidity and disfigurement greatly. Additionally, laser ablation guaranteed a better penetration for topical application of 5-aminolaevulinic acid (ALA)-PDT. Herein, we present a case of a large lesion of oral leukoplakia (OLK) in left tongue dorsum and lateral margin, pathologically manifested as severe epithelial dysplasia (SED). We firstly discussed the feasibility of Er: YAG laser assisted PDT for the treatment of SED in OPMDs. The patient achieved complete remission at 1 year follow-up. Downregulated number of p53 and Ki67 positive cells were observed in the tissues after Er: YAG laser assisted PDT. In addition, increased CD8+ positive cells infiltrated around the tissues and increased natural killer (NK) cells level were detected in the peripheral blood. In summary, Erbium:yttrium-aluminum-garnet (Er:YAG) laser assisted PDT is an effective and promising treatment for the management of SED in OPMDs with innate and adaptive immune responses.

Identification of the anti-inflammatory quality markers of Chrysanthemi Flos (Juhua) using spectrum-effect relationships combined with bioactivity re-evaluation.

Chrysanthemi Flos (Juhua), an edible herbal medicine that possesses efficacies of dispersing wind, clearing heat and detoxifying. Studies have demonstrated that the health benefits of Chrysanthemi Flos are largely attributable to its anti-inflammatory effects. However, the correlation between the compounds monitored by the current quality control methods and the anti-inflammatory effects of Chrysanthemi Flos is unclear. In order to better control the quality of Chrysanthemi Flos, the identification of anti-inflammatory quality markers (Q-markers) of Chrysanthemi Flos was performed. The chemical components of Chrysanthemi Flos were profiled by HPLC fingerprints combined with chemometrics methods. Simultaneously, the anti-inflammatory activities of 10 batches of water extracts of Chrysanthemi Flos were evaluated in lipopolysaccharide-activated RAW 264.7 macrophages cells. Gray correlation analysis was performed to assess the relationship between the anti-inflammatory activity and chemical properties. The results showed that 13 common peaks were closely correlated with the anti-inflammatory effect, and further bioactivity re-evaluation confirmed that 10 known compounds exerted a strong anti-inflammatory effect. The quantitative analysis of the 10 Q-markers showed that the 25 batches of samples could be discriminated into different zones according to their producing areas. Conclusively, the present work identified 10 anti-inflammatory Q-markers of Chrysanthemi Flos using spectrum-effect relationships combined with bioactivity re-evaluation.

Spatiotemporal heterogeneity of nitrogen transformation potentials in a freshwater estuarine system.

Under the influence of water diversion, the microbial community composition of estuarine waters and sediments might have complex spatiotemporal variations. Microbial interactions with N are significant for lake water quality. Therefore, the largest lake receiving seasonal water diversion in the North China Plain was selected as the study area. Based on 16S rRNA high-throughput sequencing and metagenomic sequencing techniques, this study analysed temporal (June-December) and spatial (estuary-pelagic zone) changes in the microbial community and functional gene composition of water and sediment. The results showed that the water microbial community composition had temporality, while sediment microbes had spatiality. The main causes of temporality in the aquatic microbial community were temperature and nitrate-N concentration, while those of sediment were flow velocity and N content. Additionally, there were complex interactions between microbial communities and N. In water, temporal variation in the relative abundance of N-related functional genes might have indirectly contributed to inorganic N composition in June (nitrite-N > ammonia-N > nitrate-N) and August (nitrite-N > nitrate-N > ammonia-N). High nitrate-N concentrations in December influenced the microbial community composition. In sediment, the estuary had higher N functional genes than the pelagic estuary, creating a relatively active N cycle and reducing total N levels in the estuary. This study revealed a potentially overlooked N sink and a flow velocity threshold that has great impacts on microbial community composition. This research contributes to a deeper understanding of the estuarine N cycle under the influence of water diversions, with implications for the calculation of global N balances and the management of lake water environments.

A combination treatment of drug-laser-photon for melasma: A retrospective study of clinical cases.

BACKGROUND: Combinational therapy such as taking tranexamic acid while using laser treatment has been proved potential efficacy by many experiments. However, there is few research which contains large samples and consistent observations. OBJECTIVE: We evaluated clinical efficacy and safety of a new systemic treatment of drug-laser-photon therapy. METHODS: Retrospective and randomized investigator-blinded study of 75 patients with mixed type melasma was analyzed. At each visit, standardized photographs were taken using VISIA. Modified melasma area and severity index (mMASI) scores were marked using photographs by two dermatologists. RESULTS: The mMASI score decreased significantly from 6.92 to 3.84 after the treatment. The VISIA analyze right cheek data shows: Spots (from 49.67 ± 3.43 to 56.09 ± 3.31), UV spots (from 41.39 ± 24.45 to 44.56 ± 25.86), and Brown spots (from 23.97 ± 17.89 to 28.16 ± 21.28) are statistically increased (p = 0.035, p = 0.018, p = 0.07). All patients feel varying degrees of improvement, about 10.17% felt very much improved, 30.51% felt much improved (51%-75%), 45.76% felt moderately improved (26%-50%), and 13.56% felt little improved (1%-25%). LIMITATIONS: This study was no control group. CONCLUSION: The efficacy and safety profile of the combination of drug-laser-photon therapy systemic treatment in melasma patients has been proved. It has potential possibility to become a new, reliable, widely suitable therapy strategy.

Antibacterial Activity of Dihydroquercetin Separated from Fructus Polygoni orientalis against Clavibacter michiganensis subsp. sepedonicus via Damaging Cell Membrane.

The yield and quality of potato can be severely affected by bacterial ring rot, which is caused by Clavibacter michiganensis subsp. sepedonicus (Cms). Recently, using natural compounds to control bacteria has received more attention. In this study, five antibacterial compounds from ethyl acetate (EtOAc) extract of Fructus Polygoni orientalis (FPO) against Cms were isolated and the most active compound was screened. Five active compounds were identified as 3,3'-di-O-methylellagic acid (1), 3,3'-di-O-methylellagic acid-4-O-ß-D-glucopyranoside (2), dihydroquercetin (3), protocatechuic acid (4) and quercetin (5). Compound 3 (dihydroquercetin, DHQ) was confirmed as the most active compound. The diameter of inhibition zone (DIZ), minimum inhibitory concentration (MIC), protective efficiency and curative efficiency of DHQ were 22.50 mm, 0.313 mg/mL, 84.49% and 79.63%, respectively, which exceeded these of thiophanate-methyl (TM) in antibacterial activity assays; this indicated that DHQ had satisfactory antibacterial activities against Cms in vitro and in vivo. Results of cell membrane damage assessments indicated that DHQ could reduce membrane potential (MP), disrupt the cell membrane integrity, and promote the leakage of nucleic acids and proteins. Overall, these findings suggested that DHQ could serve as a promising lead molecular against Cms, which could provide a basis for its further derivatization.

Dietary ß-hydroxybutyric acid improves the growth performance of young ruminants based on rumen microbiota and volatile fatty acid biosynthesis.

Introduction: The ketone body ß-hydroxybutyric acid (BHB) plays critical roles in cellular proliferation and metabolic fuel utilization; however, its effects on the rumen microbiota remain unknown. Methods: Here, three doses of BHB (low, medium, and high) were supplemented to early-weaned goat kids. Results: Compared with controls, the beneficial effects of BHB on growth and rumen development were observed in goats at 90 days of age (d). The low dose of dietary BHB increased the concentration of rumen acetate, propionate, and butyrate on d90. The sequencing results of the rumen microbiota revealed marked shifts in rumen microbial community structure after early-weaned goat kids consumed BHB for 2 months. The signature bacterial ASVs for each treatment were identified and were the main drivers contributing to microbial interactions in the rumen. The bacteria associated with rumen weight were also correlated with body weight. Some classified bacterial signatures, including Prevotella, Olsenella umbonate, and Roseburia faecis, were related to rumen volatile fatty acids and host development. Conclusion: Overall, dietary BHB altered rumen microbiota and environments in young goats, which contributed to rumen development and growth.

[Pharmacodynamic material basis and anti-inflammatory mechanism of Chrysanthemum morifolium cv. Fubaiju based on UPLC-Q-TOF-MS/MS combined with network pharmacology].

The chemical components in rats after oral administration of the water extract of Chrysanthemum morifolium cv. Fubaiju(CMF) were analyzed by UPLC-Q-TOF-MS/MS technique. Forty-four compounds were identified from the water extract of CMF and 11 components were identified from the rat serum. A total of 264 potential anti-inflammatory targets were identified by network pharmacology based on serum components. The "component-target" network and protein-protein interaction(PPI) network were constructed, and GO function enrichment and KEGG pathway enrichment analyses were performed. The molecular docking was carried out to validate the results of network pharmacology. The results showed that CMF might act on AKT1, TNF, TP53, IL6, INS, and other core targets through apigenin, luteolin, acacetin, diosmetin, 3,4-O-dicaffeoylquinic acid, and other active components, and exert anti-inflammatory effects by regulating PI3 K-AKT signaling pathway, FoxO signaling pathway, cAMP signaling pathway, Ras signaling pathway, and other pathways. The pharmacodynamic materials basis of CMF was identified by UPLC-Q-TOF-MS/MS technology, and the core anti-inflammatory targets and the underlying mechanism of action were analyzed by network pharmacology and molecular docking, which provided a reference for comprehensively clarifying the pharmacodynamic materials basis and quality control of CMF.

Uncovering the effect of polyethyleneimine on methane production in anaerobic digestion of sewage sludge.

The potential effect of polyethyleneimine as a flocculant on anaerobic digestion of sludge was investigated. Polyethyleneimine above 12 g/kg total suspended solids inhibited the entire anaerobic digestion process including solubilization, hydrolysis, acidification, and methanogenesis. The addition of 24 g/kg total suspended solids polyethyleneimine reduced methane production from 167 ± 5 L/kg volatile suspended solids in the control reactor (without polyethyleneimine) to 141 ± 5 L/kg volatile suspended solids. Polyethyleneimine bound to extracellular polymeric substances, thus enhancing sludge agglomeration and hindering the release of organics. Meanwhile, the reduction of cytochrome C impeded electron transport, consequently curbed direct interspecies electron transfer. The adsorption of carbon dioxide by amine groups also hampered methane conversion. This study elucidated the concept that polyethyleneimine reduces mass transfer in anaerobic digestion, providing new insights into the potential behavior of flocculants in sludge treatment.

Antibacterial Activity and Mechanism of Polygonum orientale L. Essential Oil against Pectobacterium carotovorum subsp. carotovorum.

Infected by Pectobacterium carotovorum subsp. carotovorum (Pcc), the quality of Chinese cabbage could severely decline. Using chemical bactericides to control Pcc could cause food safety problems. Thus, we investigated the optimum extraction conditions, antibacterial activity, chemical compounds and antibacterial mechanism of Polygonum orientale L. essential oil (POEO) against Pcc in order to search a new way to control Pcc. The optimum extraction conditions of POEO (soaking time 2.6 h, extraction time 7.7 h and ratio of liquid to solid 10.3 mL/g) were optimized by response surface methodology. The minimum inhibitory concentration (MIC) of POEO against Pcc was 0.625 mg/mL. The control efficiency of protective activity of POEO against Pcc was 74.67~92.67%, and its curative activity was 76.00~93.00%. Then, 29 compounds were obtained by GC-MS; the prime compounds of POEO were phytol, phytone, n-pentacosane, 1-octen-3-ol and ß-ionone. It was verified that, compared with control samples, POEO destroyed cell morphology. It increased surface potential, increased hydrophobicity, damaged cell walls, destroyed the integrity and permeability of cell membrane, reduced membrane potential (MP), and changed membrane protein conformation. It inhibited the activities of pyruvate kinase (PK), succinate dehydrogenase (SDH) and adenosine triphosphatase (ATPase). Briefly, the results of this study demonstrate that POEO showed effective inhibitory activity against Pcc, thus POEO could have potential application in controlling Pcc.

Slight flow volume rises increase nitrogen loading to nitrogen-rich river, while dramatic flow volume rises promote nitrogen consumption.

Concentrated rainfall and water transfer projects result in slight and dramatic increases in flow volume over short periods of time, causing nitrogen recontamination in the water-receiving areas of nitrogen-rich rivers. This study coupled hydrodynamic and biochemical reaction models to construct a model for quantifying diffusive transport and transformation fluxes of nitrogen across the water-sediment interface and analysed possible changes in the relative abundance of microbial functional genes using high-throughput sequencing techniques. In this study, the processes of ammonium (NH4+-N) and nitrate (NO3--N) nitrogen release and sedimentation with resuspended particles, as well as mineralisation, nitrification, and denitrification processes were investigated at the water-sediment interface in the Fu River during slight and dramatic increases in flow volume caused by concentrated rainfall and water diversion projects. Specifically, a slight flow volume rise increased the release of NH4+-N from the sediment, inhibited sedimentation of NO3--N, decreased the mineralisation rate, increased the nitrification rate, and had little effect on the denitrification process, ultimately increasing the nitrogen load to the river water. A dramatic increase in flow volume simultaneously increased NH4+-N and NO3--N exchange fluxes, inhibited the mineralisation process, promoted nitrification-denitrification processes, and increased inorganic nitrogen consumption in the river. This study provides a solution for the re-pollution of rivers that occurs during the implementation of reservoir management and water diversion projects. Furthermore, these results indicate a potential global nitrogen sink that may have been overlooked.

Total Triterpenes of Wolfiporia cocos (Schwein.) Ryvarden & Gilb Exerts Antidepressant-Like Effects in a Chronic Unpredictable Mild Stress Rat Model and Regulates the Levels of Neurotransmitters, HPA Axis and NLRP3 Pathway.

Purpose: Wolfiporia cocos is frequently used in traditional Chinese medicine to treat depression. However, antidepressant-like effects of the main active ingredients of Wolfiporia cocos, total triterpenes of Wolfiporia cocos (TTWC), are not well studied. This study aimed to investigate those effects and explore their specific mechanisms of action in depth. Methods: Chemical components of TTWC were analyzed using LC-MS. Depression-like behavior in rats were induced by chronic unpredictable mild stress (CUMS). The suppressive effects of TTWC (60, 120, 240 mg/kg) against CUMS-induced depression-like behavior were evaluated using the forced swimming test (FST), open field test (OFT) and sucrose preference test (SPT). Levels of 5-hydroxytryptamine (5-HT), glutamate (GLU), corticotropin-releasing hormone (CRH), interleukin-1 beta (IL-1beta), interleukin-18 (IL-18), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in different groups were determined by ELISA. Western blotting (WB) was used to detect the expression of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1beta, IL-1beta, pro-IL-18, and IL-18 in the prefrontal cortex. Additionally, the mRNA levels of NLRP3, ASC, caspase-1, IL-1beta and IL-18 were detected by RT-PCR. Results: A total of 69 lanostane-type triterpene acids of TTWC were identified. The results showed that TTWC exhibited an antidepressant-like effect in CUMS rats, reversed the decreased sugar preference in the SPT, reduction of immobility time in the FST, reduced the rest time, increased the total moving distance in the OFT. TTWC increased 5-HT levels and decreased GLU levels in the hippocampus. Moreover, TTWC decreased CRH levels in serum, indicating the regulation of over-activation of the hypothalamic-pituitary-adrenal (HPA) axis. In addition, reduced serum levels of IL-1beta, IL-18, IL-6, and TNF-alpha. The WB results implied that TTWC inhibited the expression of NLRP3, ASC, caspase-1, IL-1beta, and IL-18 in the prefrontal cortex and enhanced the expression of pro-caspase-1, pro-IL-1beta, and pro-IL-18. Although most of the results were not significant, PCR results showed that TTWC inhibited the expression of NLRP3, ASC, caspase-1, IL-1beta, and IL-18 in the prefrontal cortex. Conclusion: TTWC treatment exerted an antidepressant-like effect and regulates neurotransmitters, HPA axis and NLRP3 signaling pathway. These results indicated the potential of TTWC in preventing the development of depression.

The Temporal Dynamics of Rumen Microbiota in Early Weaned Lambs.

Weaning affects the development of ruminal bacteria in lambs during early life. However, the temporal dynamics of rumen microbiota in early weaned lambs is unknown compared to conventionally weaned lambs. In this study, one group was reared with their dams (control, CON) and conventionally weaned at 49 days (d), while the other lambs were weaned at 21 d (early weaning, EW) using starter. Rumen microbial samples collected at 26, 35, and 63 d were used for next-generation sequencing. Here, we found that the abundance and diversity of rumen microbiota in EW were significantly lower at 26 and 35 d than the CON. Linear discriminant analysis Effect Size (LEfSe) analysis was performed to identify the signature microbiota for EW at these three ages. At 26 d, Prevotella 7, Syntrophococcus, Sharpea, Dialister, Pseudoscardovia, and Megasphaera in the rumen of the EW group had greater relative abundances. At 35 d, the Lachnospiraceae_NK3A20_group was enriched in CON. On 63 d, Erysipelotrichaceae_UCG-002 was abundant in EW. Syntrophococcus and Megaspheaera in EW lambs were abundant at 26 and 35 d, but kept similar to CON at 63 d. The relative abundance of Erysipelotrichaceae_UCG-002 at all-time points was consistently higher in the EW group. In conclusion, early weaning led to a significant decrease in rumen microbiota richness and diversity in the short term. The changes in rumen microbiota are associated with the persistence of weaning stress. The temporal dynamics of relative abundances of Syntrophococcus, Megasphaera, and Ruminococcaceae_UCG-014 reflect the weaning stress over a short period and rumen recovery after early weaning.

[Dynamic Mechanisms of Groundwater Quality by Residual Contaminants of the Tanghe Wastewater Reservoir in Xiong'an New Area].

Tanghe wastewater reservoir(TWR) is located on the west side of Baiyangdian Lake in Xiong'an New Area, where sewage infiltration and irrigation has been taking place for 40 years, and a large number of contaminants have accumulated in the unsaturated zone. Identifying the mechanisms by which this combined system contributes to groundwater hydrochemical dynamics is important for the protection of the water environment in the area. Hydrogeochemical methods such as tracing and improved chlor-alkali index are used to analyze the spatial and temporal characteristics and evolution mechanisms of shallow groundwater. The study shows that the groundwater chemistry in the sewage reservoir area is SO4·HCO3-Na type, with an average sewage fraction of 48.4%, and the contribution of Na+ from ion exchange and halite dissolution is 29.9% and 8.6%, respectively. The chemical type of groundwater in the sewage irrigation area is SO4·HCO3-Na·Mg, the average sewage fraction is 58.3%, and Na+ consumption of ion exchange is 8.1%. The mix dilution of precipitation and irrigation leads to a reduction in the effluent fraction and saturation index in the groundwater, and promotes the adsorption of Na+ from groundwater into the soil. Denitrification in aquifers can effectively reduce groundwater nitrate pollution. In addition, the sewage fraction before and after the restoration of the reservoir was 61.5% and 49.3%, respectively. Pollutants retained in the sewage infiltration and irrigation combined system will continue to affect the quality of shallow groundwater with varying degrees of mixing and water-rock interaction driven by rainfall and irrigation.

Melatonin Ameliorates the Toxicity Induced by Deoxynivalenol in Murine Ovary Granulosa Cells by Antioxidative and Anti-Inflammatory Effects.

Melatonin is an important endogenous hormone that shows antioxidant functions and pleiotropic effects, playing a crucial role in animal reproduction. Ovary granulosa cells (GCs) surround the oocyte, which play an important role in regulating oocytes development. Deoxynivalenol (DON) is a common fusarium mycotoxin contaminant of feedstuff and food, posing a serious threat to human and animal reproductive systems. Herein, murine ovary GCs were studied as a reproduction cell model, aimed to assess the protective effect of melatonin on DON-induced toxicity in murine ovary GCs. The results showed that DON adversely affected the viability and growth of murine ovary GCs and increased the apoptosis rate, while melatonin administration ameliorated these toxic effects. We further reveal that DON exposure increased the intracellular reactive oxygen species level, reduced the mitochondrial membrane potential and ATP, and upregulated Tnfα (tumor necrosis factor α), Il6 (interleukin 6), and Il1ß (interleukin 1 ß) gene expression. Moreover, DON exposure downregulated reproductive hormone gene expression and significantly increased nuclear factor kappa B (p65) activation and mitogen-activated protein kinase phosphorylation. Melatonin treatment attenuated all these effects, suggesting that melatonin protects GCs from the adverse effects of DON by ameliorating oxidative stress, mitochondrial dysfunction, and inflammation. Overall, these results reveal the mechanisms of DON and melatonin in GCs and provide a theoretical basis for melatonin as a drug to improve mycotoxin contamination.