Interleukin-34 Synovial Fluid Was Associated with Knee Osteoarthritis Severity: A Cross-Sectional Study in Knee Osteoarthritis Patients in Different Radiographic Stages.

BACKGROUND: Inflammation might play a crucial role in the pathogenesis of osteoarthritis (OA). Interleukin-34 (IL-34) is a well-known proinflammatory cytokine. OBJECTIVE: The objective of this study was to detect IL-34 levels in serum and synovial fluid (SF) of patients with OA and to investigate their correlation with radiographic and symptomatic severity. METHODS: One hundred and eighty-two OA patients and 69 controls were recruited. IL-34 levels were measured by enzyme-linked immunosorbent assay (ELISA). Radiographic and symptomatic severity of OA was reflected by Kellgren-Lawrence (KL) grades and Western Ontario McMaster University Osteoarthritis Index (WOMAC) scores, respectively. RESULTS: SF IL-34 levels were independently associated with the KL grade (B = 0.273, 95% CI: 0.150-0.395; P < 0.001). SF IL-34 levels were significantly correlated with WOMAC scores (r = 0.265, 95% CI: 0.123-0.399; P < 0.001). The correlation between SF IL-34 levels and WOMAC scores was still significant after adjusting for confounding factors (B = 0.020, 95% CI: 0.001-0.038; P = 0.035) in OA patients. CONCLUSIONS: We found that IL-34 levels in SF were significantly associated with the radiographic and symptomatic severity of knee OA.

[Role of substance P and calcitonin gene-related peptide in bone metabolism].

Substance P (SP) and calcitonin gene-related peptide (CGRP) are the neuropeptides released from the sensory nerve endings. Neuropeptides play a role in bone and the relevant organs. It exerts functions in regulation of the bone metabolism, fracture healing and pain by a certain way. The biological properties and distributions of SP and CGRP are closely related to the pathogenesis and development of bone metabolism, fracture healing and pain.