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2.
bioRxiv ; 2024 Mar 21.
Artículo en Inglés | MEDLINE | ID: mdl-38562900

RESUMEN

Motile cilia have essential cellular functions in development, reproduction, and homeostasis. Genetic causes for motile ciliopathies have been identified, but the consequences on cellular functions beyond impaired motility remain unknown. Variants in CCDC39 and CCDC40 cause severe disease not explained by loss of motility. Using human cells with pathological variants in these genes, Chlamydomonas genetics, cryo-electron microscopy, single cell RNA transcriptomics, and proteomics, we identified perturbations in multiple cilia-independent pathways. Absence of the axonemal CCDC39/CCDC40 heterodimer results in loss of a connectome of over 90 proteins. The undocked connectome activates cell quality control pathways, switches multiciliated cell fate, impairs microtubule architecture, and creates a defective periciliary barrier. Both cilia-dependent and independent defects are likely responsible for the disease severity. Our findings provide a foundation for reconsidering the broad cellular impact of pathologic variants in ciliopathies and suggest new directions for therapies.

3.
Cell ; 187(8): 1907-1921.e16, 2024 Apr 11.
Artículo en Inglés | MEDLINE | ID: mdl-38552624

RESUMEN

Hydroxyproline-rich glycoproteins (HRGPs) are a ubiquitous class of protein in the extracellular matrices and cell walls of plants and algae, yet little is known of their native structures or interactions. Here, we used electron cryomicroscopy (cryo-EM) to determine the structure of the hydroxyproline-rich mastigoneme, an extracellular filament isolated from the cilia of the alga Chlamydomonas reinhardtii. The structure demonstrates that mastigonemes are formed from two HRGPs (a filament of MST1 wrapped around a single copy of MST3) that both have hyperglycosylated poly(hydroxyproline) helices. Within the helices, O-linked glycosylation of the hydroxyproline residues and O-galactosylation of interspersed serine residues create a carbohydrate casing. Analysis of the associated glycans reveals how the pattern of hydroxyproline repetition determines the type and extent of glycosylation. MST3 possesses a PKD2-like transmembrane domain that forms a heteromeric polycystin-like cation channel with PKD2 and SIP, explaining how mastigonemes are tethered to ciliary membranes.


Asunto(s)
Chlamydomonas reinhardtii , Cilios , Glicoproteínas , Cilios/química , Glicoproteínas/química , Glicosilación , Hidroxiprolina/química , Plantas/metabolismo , Chlamydomonas reinhardtii/química
5.
Cell ; 186(13): 2880-2896.e17, 2023 06 22.
Artículo en Inglés | MEDLINE | ID: mdl-37327785

RESUMEN

Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.


Asunto(s)
Motilidad Espermática , Cola del Espermatozoide , Masculino , Animales , Bovinos , Cola del Espermatozoide/química , Cola del Espermatozoide/metabolismo , Semen , Microtúbulos/metabolismo , Axonema/química , Espermatozoides , Mamíferos
6.
Anal Chem ; 95(4): 2253-2259, 2023 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-36638820

RESUMEN

Double spike (DS) method has been extensively used in determining stable isotope ratios of many elements. However, challenges remain in obtaining high-precision isotope data for ultra-trace elements owing to the limitations of instrumental signal-to-noise ratios and the systematics of precision of DS-based measurements. Here, the DS-standard addition (SA) (DSSA) technique is proposed to improve measurements of isotope compositions of ultra-trace elements in natural samples. According to the U-shaped relationship between DS measurement uncertainty and the spike/sample ratio, theoretical equations and an error propagation model (EPM) were constructed comprehensively. In our method, a spiked secondary standard solution with a high, precisely known spike/sample ratio is mixed with samples such that the mixtures have spike/sample ratios within the optimal range. The abundances of the samples relative to the added standards (sample fraction; fspl) and the samples' isotope ratios can then be obtained exactly using a standard DS data reduction routine and the isotope binary mixing model. The accuracy and precision of the DSSA approach were verified by measurements of cadmium and molybdenum isotopes at as low as 5 ng levels. Compared with traditional DS measurements, the sample size for isotope analysis is reduced to 1/6-1/5 of the original with no loss of measurement precision. The optimal mixing range fspl = 0.15-0.5 is recommended. The DSSA method can be extended to isotope measurement of more than 33 elements where the DS method is applicable, especially for the ultra-trace elements such as platinum group and rare earth element isotopes.

7.
J Clin Immunol ; 42(8): 1672-1684, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-35849269

RESUMEN

PURPOSE: CD81 deficiency is an extremely rare primary immunodeficiency disease characterized by severe and recurrent infections, IgA-related nephropathy, and profound hypogammaglobulinemia. Only one patient has been reported so far, and the pathogenesis remains unclear. Here, we identified a new case of CD81 deficiency and described its pathogenesis. METHODS: We analyzed the clinical, genetic, and immunological features of the patient with CD81 deficiency, and explored the pathogenesis of her antibody deficiencies. RESULTS: The major manifestation of this patient was unexpectedly not recurrent infections but IgA nephropathy with aberrant serum galactose-deficient IgA1. Whole-exome sequencing revealed novel biallelic mutations in CD81 gene that abolished the surface expression of CD81. B cells from the patient lack membrane CD19 and showed reduced switched memory B cells and transitional B cells. Decreased expression of key molecules pY and pBTK in BCR signaling were demonstrated by confocal microscopy. RNA sequencing revealed that genes associated with BCR signaling and immunoglobulins were downregulated in CD81-deficient B cells. In addition, the patient showed increased frequency of T follicular helper cells that biased to Th1-like subsets. CONCLUSION: We reported the second patient with CD81 deficiency in the world and illustrated aberrant BCR signaling in the patient, therefore helping to unravel the mechanism of antibody deficiency in CD81-deficient patients.


Asunto(s)
Glomerulonefritis por IGA , Tetraspanina 28 , Femenino , Humanos , Antígenos CD19/metabolismo , Linfocitos B , China , Glomerulonefritis por IGA/diagnóstico , Glomerulonefritis por IGA/genética , Glomerulonefritis por IGA/metabolismo , Inmunoglobulina A/genética , Mutación , Tetraspanina 28/genética
8.
Nat Struct Mol Biol ; 29(5): 483-492, 2022 05.
Artículo en Inglés | MEDLINE | ID: mdl-35578023

RESUMEN

A pair of extensively modified microtubules form the central apparatus (CA) of the axoneme of most motile cilia, where they regulate ciliary motility. The external surfaces of both CA microtubules are patterned asymmetrically with large protein complexes that repeat every 16 or 32 nm. The composition of these projections and the mechanisms that establish asymmetry and longitudinal periodicity are unknown. Here, by determining cryo-EM structures of the CA microtubules, we identify 48 different CA-associated proteins, which in turn reveal mechanisms for asymmetric and periodic protein binding to microtubules. We identify arc-MIPs, a novel class of microtubule inner protein, that bind laterally across protofilaments and remodel tubulin structure and lattice contacts. The binding mechanisms utilized by CA proteins may be generalizable to other microtubule-associated proteins. These structures establish a foundation to elucidate the contributions of individual CA proteins to ciliary motility and ciliopathies.


Asunto(s)
Axonema , Microtúbulos , Axonema/metabolismo , Cilios/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismo , Tubulina (Proteína)/metabolismo
9.
Environ Sci Pollut Res Int ; 29(43): 64721-64731, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35474432

RESUMEN

High gravity technology, as a process intensification technology, has demonstrated the great advantages in the field of gas purification on account of its excellent mass transfer efficiency and energy-efficient, but it is rarely applied in the field of nitrogen oxides (NOx) purification of marine diesel engine exhaust. In this paper, a high-gravity bowl-shaped-disk rotating bed (HBRB) without catalytic was designed for diesel exhaust after-treatment. A diesel oxidation catalyst (DOC) was installed in the front of the HBRB to regenerate more nitrogen dioxide (NO2) easily reduced by urea. A bench test of a 6-cylinder marine diesel engine with the HBRB was carried out. The effects of the HBRB speeds, urea concentrations, and engine operating conditions on NOx purification efficiency in engine exhaust were experimentally investigated. The experimental result indicates that the maximum NOx removal efficiency of the HBRB can reach 69.1%. The improvement of the NOx removal efficiency is not obvious at the HBRB speed of over 1500 r/min. The pre-oxidation degree of nitric oxide (NO) and urea concentration largely affect the NOx removal efficiency. The HBRB has great potential in marine diesel engine exhaust denitration.


Asunto(s)
Hipergravedad , Emisiones de Vehículos , Gasolina , Óxido Nítrico , Dióxido de Nitrógeno/análisis , Óxidos de Nitrógeno/análisis , Urea
10.
Avian Dis ; 65(2): 261-268, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-34412457

RESUMEN

The resistance to serum complement-mediated killing is a vital virulence property of microbial pathogens. Complement factor H (FH) is a key negative regulator of the complement alternative pathway (AP) that prevents formation and accelerates the decay of AP C3 convertase and acts as a cofactor in the inactivation of C3b. Pathogens can recruit host FH through their surface proteins to escape the clearance of the complement system. Riemerella anatipestifer could also evade the complement system attack to achieve host infection, but the mechanism is still unclear. In this study, the R. anatipestifer proteins that could interact with FH in host serum were screened and analyzed, and the functions were determined. Affinity chromatography with a Ni-nitrilotriacetic acid Sefinose column and mass spectrometry identified three outer membrane proteins (Omp) of R. anatipestifer, Omp54, Omp53, and Omp24, as potential FH-binding proteins. We then successfully conducted the prokaryotic expression and polyclonal antibody preparation of three candidate proteins. Indirect immunofluorescence assay showed that three candidate proteins were all present in R. anatipestifer. The affinity blotting assay, anti-serum-inhibiting assay, and serum bactericidal assay presented evidence that Omp24 could bind FH. Moreover, FH bound to Omp24 was associated with resistance to the alternative pathway and functional for R. anatipestifer survival in the normal duck serum. These results suggested that R. anatipestifer Omp24 was a FH-binding protein and the interaction with FH blocked the alternative pathway. Recruitment of complement regulatory proteins may facilitate better R. anatipestifer resistance to this vital line of host defense.


Artículo regular­El factor H del complemento de pato se une a la proteína de la membrana externa Omp24 de Riemerella anatipestifer La resistencia a la destrucción mediada por el complemento sérico es una propiedad vital para la virulencia de los patógenos microbianos. El factor de complemento H (FH) es un regulador negativo clave de la vía alterna del complemento (AP) que previene la formación y acelera la descomposición de la C3 convertasa de la vía alterna y actúa como cofactor en la inactivación de C3b. Los patógenos pueden reclutar factor H del huésped a través de sus proteínas de superficie para escapar de la destrucción por el sistema del complemento. Riemerella anatipestifer también pudo evadir el ataque del sistema del complemento para lograr la infección del huésped, pero el mecanismo aún no está claro. En este estudio, se seleccionaron y analizaron las proteínas de R. anatipestifer que podrían interactuar con el factor H en el suero del huésped y se determinaron las funciones. La cromatografía de afinidad con una columna de sefinosa de Ni-NTA y la espectrometría de masas identificaron tres proteínas de la membrana externa de R. anatipestifer, Omp54, Omp53 y Omp24, como posibles proteínas que se unen al factor H. Posteriormente, se llevó a cabo con éxito la expresión procariota y la preparación de anticuerpos policlonales de las tres proteínas candidatas. El ensayo de inmunofluorescencia indirecta mostró que las tres proteínas candidatas estaban presentes en R. anatipestifer. El ensayo de transferencia para afinidad, el ensayo anti-inhibidor del suero y el ensayo bactericida sérico presentaron evidencia de que la proteína Omp24 podría unirse al factor H. Además, el factor H unido a la proteína Omp24 se asoció con resistencia a la vía alterna y funcional para la supervivencia de R. anatipestifer en el suero de pato normal. Estos resultados sugirieron que la proteína Omp24 de R. anatipestifer era una proteína de unión al factor H y que la interacción con este factor bloqueaba la vía alterna del complemento. El reclutamiento de proteínas reguladoras del complemento puede facilitar una mejor resistencia de R. anatipestifer a esta línea vital de defensa del huésped.


Asunto(s)
Proteínas de la Membrana Bacteriana Externa/metabolismo , Factor H de Complemento/metabolismo , Riemerella/metabolismo , Secuencia de Aminoácidos , Animales , Proteínas de la Membrana Bacteriana Externa/química , Cromatografía de Afinidad/veterinaria , Patos , Técnica del Anticuerpo Fluorescente Indirecta/veterinaria , Ratones , Conejos , Riemerella/inmunología
12.
Sci Total Environ ; 783: 147126, 2021 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-34088147

RESUMEN

Black shale contains abundant pyrite and organic matter that are susceptible to weathering when exposed to the air. In the process of weathering, acid mine drainage can be produced, and a range of toxic trace elements including Cr can be released and transported into rivers, groundwater and soils, potentially leading to severe environmental pollution. In order to study Cr migration and Cr isotopic fractionation during black shale weathering, we sampled metalliferous black shales and cherts from two weathering profiles at Shadi and Yutangba from the Permian Maokou Formation in Enshi Prefecture. The unweathered samples in Shadi and Yutangba have high Cr contents (1562 µg/g and 643 µg/g, respectively), and highly fractionated Cr isotopic compositions (2.04 ± 0.11‰ and 1.91 ± 0.09‰, respectively, expressed as δ53Cr). The narrow range of authigenic δ53Cr in these unweathered shales suggests that the δ53Cr value of the seawater was relatively stable during the period of deposition. Strongly weathered shales in Shadi and Yutangba both display significant Cr losses compared to the unweathered counterparts. Their average δ53Cr values are 1.75 ± 0.12‰ and 1.85 ± 0.39‰ for Shadi and Yutangba, respectively, which are isotopically lighter than fresh samples. This indicates that heavier Cr isotopes are preferentially leached into fluids, leaving the residues enriched in lighter isotopes during black shale weathering. However, the δ53Cr values of the samples close to the water table are higher than those of the unaltered ones, which can be explained by adsorption or quantitative reduction of Cr(VI) near the water table. The fact that Cr isotopes are fractionated during black shale weathering may complicate the application of δ53Cr in polluted samples to identify the Cr sources in areas with exposed black shales. The δ53Cr of seepage water can be measured and treated as a more realistic source signal.

13.
Anal Chem ; 93(20): 7449-7455, 2021 05 25.
Artículo en Inglés | MEDLINE | ID: mdl-33974413

RESUMEN

Stable isotope ratios are widely used to solve environmental, geological, medical, and forensic problems. The double spike technique is considered to be one of the most robust and efficient methods to correct for instrumental mass bias and isotopic fractionation that may occur during sample preparation. However, various hidden errors can arise from data processing and have been largely overlooked in previous studies. Several of these hidden errors were investigated in this work using measurement and synthetic data. Double spike inversion of chromium isotope raw data from 1116 natural samples demonstrated that averaging raw isotope ratios before double spike inversion can add significant errors to inverted isotope values, and such errors can be 1.5 times larger than the true analytical precision. Synthetic data were used to investigate the errors on inverted Cr isotope data caused by spike:analyte ratio and Fe-Ti-V interferences, and the following threshold values are recommended to minimize such errors: 54Crspike/52Crsample ratio greater than 0.5, 56Fe/52Cr less than 0.2, 49Ti/52Cr less than 0.04, and 51V/52Cr less than 1. Sample preparation can potentially lead to large errors in inverted Cr isotope data if preparation-induced isotope fractionation deviates from the exponential law used in the double spike inversion, but such errors can be minimized by achieving >70% Cr yield. Our findings provide important insights for the double spike inversion procedure and assessing the reliability of inverted isotope data for not only the chromium isotope system but also other elements commonly analyzed using the double spike technique.


Asunto(s)
Fraccionamiento Químico , Isótopos , Cromo , Isótopos de Cromo , Reproducibilidad de los Resultados
14.
Nat Commun ; 12(1): 3065, 2021 05 24.
Artículo en Inglés | MEDLINE | ID: mdl-34031406

RESUMEN

In living cells, microtubules (MTs) play pleiotropic roles, which require very different mechanical properties. Unlike the dynamic MTs found in the cytoplasm of metazoan cells, the specialized cortical MTs from Toxoplasma gondii, a prevalent human pathogen, are extraordinarily stable and resistant to detergent and cold treatments. Using single-particle cryo-EM, we determine their ex vivo structure and identify three proteins (TrxL1, TrxL2 and SPM1) as bona fide microtubule inner proteins (MIPs). These three MIPs form a mesh on the luminal surface and simultaneously stabilize the tubulin lattice in both longitudinal and lateral directions. Consistent with previous observations, deletion of the identified MIPs compromises MT stability and integrity under challenges by chemical treatments. We also visualize a small molecule like density at the Taxol-binding site of ß-tubulin. Our results provide the structural basis to understand the stability of cortical MTs and suggest an evolutionarily conserved mechanism of MT stabilization from the inside.


Asunto(s)
Microscopía por Crioelectrón , Interacciones Huésped-Parásitos/fisiología , Proteínas de Microtúbulos/ultraestructura , Microtúbulos/ultraestructura , Toxoplasma/metabolismo , Sitios de Unión , Sistemas CRISPR-Cas , Humanos , Proteínas de Microtúbulos/metabolismo , Proteínas Asociadas a Microtúbulos/química , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismo , Paclitaxel/química , Tubulina (Proteína)/química , Tubulina (Proteína)/metabolismo
15.
Medicine (Baltimore) ; 100(13): e24875, 2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33787577

RESUMEN

BACKGROUND: In recent years, with the accelerated pace of life, diet, environmental problems occur frequently. External factors are easily to cause endocrine disorders and hormone sensitivity of breast tissue, which can lead to mammary hyperplasia. The incidence rate of hyperplasia of mammary glands is increasing year by year, and the age of onset is also getting lower and lower. If not treated in time, there is a crisis of breast cancer.Clinical studies have found that auricular point pressing therapy is widely used in clinical treatment of mammary hyperplasia recently, but the efficacy of massage in the treatment of mammary hyperplasia has not been systematically reviewed. The purpose of this study is to explore the efficacy, safety and effectiveness of auricular point pressing therapy in the treatment of hyperplasia of mammary glands. METHODS: We will search PubMed, Web of Science, Cochrane Library, EMBASE, Wan fang Database, Chinese Scientific Journal Database, CNKI, VIP, and Chinese Biomedical Literature Database. The retrieval date was January 10, 2021. RevMan 5.3 software was used to evaluate the quality and risk of included studies. The efficacy, recurrence rate, and symptom score of breast hyperplasia were analyzed, and the results were observed and measured. RESULTS: This study will be from the clinical efficiency, improvement rate, pain symptoms disappear rate, tumor size improvement rate, and other aspects of the existing evidence for a high quality synthesis, as well as auricular point pressing therapy adverse events. CONCLUSION: the conclusion of this review will provide the basis for judging whether auricular point pressing therapy is safe and effective in the treatment of hyperplasia of mammary glands. ETHICS AND DISSEMINATION: This systematic will evaluate the effectiveness and safety of auricular point pressing therapy in the treatment of hyperplasia of mammary glands. As all data used in this systematic review and meta-analysis have been published, ethical approval is not required for this review. PROTOCOL REGISTRATION NUMBER: INPLASY202110028.


Asunto(s)
Puntos de Acupuntura , Enfermedades de la Mama/terapia , Glándulas Mamarias Humanas/patología , Masaje/métodos , Enfermedades de la Mama/patología , Ensayos Clínicos como Asunto , Pabellón Auricular , Femenino , Humanos , Hiperplasia/terapia , Metaanálisis como Asunto , Proyectos de Investigación , Revisiones Sistemáticas como Asunto , Resultado del Tratamiento
16.
Nat Struct Mol Biol ; 28(1): 29-37, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-33318703

RESUMEN

In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform.


Asunto(s)
Chlamydomonas reinhardtii/anatomía & histología , Cilios/metabolismo , Locomoción/fisiología , Proteínas de Plantas/metabolismo , Axonema/metabolismo , Fenómenos Biomecánicos/fisiología , Microscopía por Crioelectrón , Proteínas del Citoesqueleto/metabolismo , Dineínas/metabolismo , Flagelos/metabolismo , Microtúbulos/metabolismo , Modelos Moleculares , Estructura Terciaria de Proteína , Transducción de Señal/fisiología , Nexinas de Clasificación/metabolismo
17.
ACS Omega ; 5(36): 23460-23467, 2020 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-32954199

RESUMEN

Here, hierarchical porous nitrogen-containing activated carbons (N-ACs) were prepared with LiCl-ZnCl2 molten salt as a template derived from cheap chitosan via simple one-step carbonization. The obtained N-ACs with the highest specific surface area of 2025 m2 g-1 and a high nitrogen content of 5.1 wt % were obtained using low molten salt/chitosan mass ratio (3/1) and moderate calcination temperature (1000 °C). Importantly, using these N-ACs as CO2 solid-state adsorbents, the maximum CO2 capture capacities could be up to 7.9/5.6 mmol g-1 at 0 °C/25 °C under 1 bar pressure, respectively. These CO2 capture capacities of N-ACs were the highest compared to reported biomass-derived carbon materials, and these values were also comparable to most of porous carbon materials. Moreover, as-made N-ACs also showed good selectivity for CO2/N2 separation and excellent recyclability. The unique hierarchical porous structure of N-ACs thus provided the right combination of adsorbent properties and could enable the design of high-performance CO2 solid adsorbents.

18.
Anal Chem ; 92(1): 1463-1469, 2020 01 07.
Artículo en Inglés | MEDLINE | ID: mdl-31766840

RESUMEN

High-sensitivity and high-precision (2 SD ≤ 0.06‰) measurement of chromium (Cr) isotopes at the 10 ng level was successfully carried out using double spike multiple-collector inductively couple plasma mass spectrometry (MC-ICP-MS). To enhance the signal sensitivity and stability, the Aridus II desolvating nebulizer system was improved by placing its waste gas trap bottle in an ice chamber (5 °C cold trap). This setup, beyond Cr isotope analysis, can be applied to most heavy metal isotope measurements. The sensitivity of the 52Cr signal is ≥300 V mg-1 L (with a 1011Ω amplifier and a 110 µL min-1 uptake rate), an enhancement of ≥1.5 times compared to the Aridus II without the cold trap. In addition, the relative standard deviation of the 52Cr signal varied ≤4% over 8 h, demonstrating high stability. The δ53Cr values of common geological reference materials determined using 10 ng of Cr are in excellent agreement with results measured at 25 ng and 50 ng and are consistent with previous determinations, validating the accurate and precise Cr isotope ratio measurements. An empirical method is proposed to correct for the residual (after subtraction) effect of Fe interference on δ53Cr determination. This method relies on a linear relationship between the [Fe]/[Cr] and δ53Cr shift within one analytical session. Finally, we report the δ53Cr values of 19 new reference materials, ranging from -0.44‰ to 0.49‰. Among them, GSS-7 (-0.44 ± 0.02‰, 2 SD, n = 5), GSS-4 (0.48 ± 0.02‰, 2 SD, n = 5), and GSD-10 (0.49 ± 0.05‰, 2 SD, n = 5) can be used as candidate reference materials for interlaboratory comparisons to complement existing ones that are mostly isotopically unfractionated from the bulk silicate earth.

19.
Geobiology ; 17(6): 579-593, 2019 11.
Artículo en Inglés | MEDLINE | ID: mdl-31436043

RESUMEN

Atmospheric oxygen levels control the oxidative side of key biogeochemical cycles and place limits on the development of high-energy metabolisms. Understanding Earth's oxygenation is thus critical to developing a clearer picture of Earth's long-term evolution. However, there is currently vigorous debate about even basic aspects of the timing and pattern of the rise of oxygen. Chemical weathering in the terrestrial environment occurs in contact with the atmosphere, making paleosols potentially ideal archives to track the history of atmospheric O2 levels. Here we present stable chromium isotope data from multiple paleosols that offer snapshots of Earth surface conditions over the last three billion years. The results indicate a secular shift in the oxidative capacity of Earth's surface in the Neoproterozoic and suggest low atmospheric oxygen levels (<1% PAL pO2 ) through the majority of Earth's history. The paleosol record also shows that localized Cr oxidation may have begun as early as the Archean, but efficient, modern-like transport of hexavalent Cr under an O2 -rich atmosphere did not become common until the Neoproterozoic.


Asunto(s)
Atmósfera/análisis , Isótopos de Cromo/análisis , Oxígeno/análisis , Suelo/química , Isótopos de Cromo/química , Ontario , Oxidación-Reducción , Paleontología , Sudáfrica , Estados Unidos
20.
Phytopathology ; 109(6): 972-982, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-30714883

RESUMEN

Plant survival in the terrestrial ecosystem is influenced by both beneficial and harmful microbes. Trichoderma spp. are a group of filamentous fungi that promote plant growth and resistance to harmful microbes. Previously, we showed that the genus Trichoderma could effectively suppress Fusarium wilt in cucumber. However, the mechanisms that underlie the effects of the genus Trichoderma on plant defense have not been fully substantiated. Two essential metabolic pathways, such as the ascorbate (AsA)-glutathione (GSH) cycle and the oxidative pentose phosphate pathway (OPPP), have been shown to participate in plant tolerance to biotic stressors; nevertheless, the involvement of these pathways in Trichoderma-induced enhanced defense remains elusive. Here, we show that Trichoderma harzianum could alleviate oxidative and nitrostative stress by minimizing reactive oxygen species (ROS; hydrogen peroxide and superoxide) and reactive nitrogen species (nitric oxide [NO]) accumulation, respectively, under Fusarium oxysporum infection in cucumber roots. The genus Trichoderma enhanced antioxidant potential to counterbalance the overproduced ROS and attenuated the transcript and activity of NO synthase and nitrate reductase. The genus Trichoderma also stimulated S-nitrosylated glutathione reductase activity and reduced S-nitrosothiol and S-nitrosylated glutathione content. Furthermore, the genus Trichoderma enhanced AsA and GSH concentrations and activated their biosynthetic enzymes, γ-GCS and l-galactono-1,4-lactone dehydrogenase. Interestingly, the genus Trichoderma alleviated Fusarium-inhibited activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, enzymes involved in the OPPP. Such positive regulation of the key enzymes indicates the adequate maintenance of the AsA-GSH pathway and the OPPP, which potentially contributed to improve redox balance, energy flow, and defense response. Our study advances the current knowledge of Trichoderma-induced enhanced defense against F. oxysporum in cucumber.


Asunto(s)
Cucumis sativus , Fusarium , Enfermedades de las Plantas/microbiología , Trichoderma , Raíces de Plantas , Especies Reactivas de Oxígeno
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