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1.
Adv Mater ; 36(9): e2309046, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38011581

RESUMEN

Developing a robust strategy for profiling heterogeneous circular tumor cells specifically, distinguishing the phenotypes of which in blood sample of cancer patient precisely, and releasing them sequentially, is significant for cancer management by liquid biopsy. Herein, a bio-inspired free-standing and flexible film composed of TiO2 nanotube and silk fibroin, fabricated with multiply dynamic bioactive surface (TSF/MDBS) by a simple and eco-friendly way including using polydopamine chemistry and dual dynamic covalent chemistry, is reported. The as-prepared TSF/MDBS binds specific peptides toward cells with epithelial biomarker and human epithelial growth factor receptor 2 (HER2) biomarker, and antifouling agents bovine serum albumin for obviating platelets and proteins adhering of blood, can capture heterogeneous CTCs with enhanced capability due to the cytocompatible soft film and exquisite surface design, and further release the captured cells as program, by specifically breaking down the covalent bonds in sequence via the action of adding biocompatible molecules fructose and glutathione. By applying the TSF/MDBS, it can be tailored into desired pieces for identifying CTCs with different phenotypes (HER2-high and HER2-low) from the unprocessed blood samples of breast cancer patients, and finally profiling these heterogeneous CTCs, to discriminate HER2 positive or negative of breast cancer patients in clinical applications.


Asunto(s)
Neoplasias de la Mama , Fibroínas , Humanos , Femenino , Neoplasias de la Mama/diagnóstico , Plaquetas , Tipificación Molecular , Biomarcadores
2.
Nanotechnology ; 34(50)2023 Sep 28.
Artículo en Inglés | MEDLINE | ID: mdl-37625382

RESUMEN

Cross-fibrillation of amyloid-ß(Aß) peptides and human islet amyloid polypeptides (hIAPP) has revealed a close correlation between Alzheimer's disease and type 2 diabetes (T2D). Importantly, different amyloid strains are likely to lead to the clinical pathological heterogeneity of degenerative diseases due to toxicity. However, given the complicated cross-interactions between different amyloid peptides, it is still challenging to identify the polymorphism of the hybrid amyloid strains and reveal mechanistic insights into aggregation, but highly anticipated due to their significance. In this study, we investigated the cross-fibrillation of Aßpeptides and different hIAPP species (monomers, oligomers, and fibrils) using combined experimental and simulation approaches. Cross-seeding and propagation of different amyloid peptides monitored by experimental techniques proved that the three species of hIAPP aggregates have successively enhanced Aßfibrillation, especially for hIAPP fibrils. Moreover, the polymorphism of these morphologically similar hybrid amyloid strains could be distinguished by testing their mechanical properties using quantitative nanomechanical mapping, where the assemblies of Aß-hIAPP fibrils exhibited the high Young's modulus. Furthermore, the enhanced internal molecular interactions andß-sheet structural transformation were proved by exploring the conformational ensembles of Aß-hIAPP heterodimer and Aß-hIAPP decamer using molecular dynamic simulations. Our findings pave the way for identifying different hybrid amyloid strains by quantitative nanomechanical mapping and molecular dynamic simulations, which is important not only for the precise classification of neurodegenerative disease subtypes but also for future molecular diagnosis and therapeutic treatment of multiple interrelated degenerative diseases.

3.
Biomater Sci ; 11(15): 5232-5239, 2023 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-37338183

RESUMEN

Piezoelectric polymer nanofibers are attracting increasing attention in the stimulation of cell growth and proliferation in tissue engineering and wound healing applications. However, their intrinsic non-biodegradability in vivo hinders widespread applications in the biological fields. Herein, we designed, synthesized and characterized composite materials of silk fibroin (SF)/LiNbO3 (LN) nanoparticles/MWCNTs by electrospinning technology, which displayed good biocompatibility and comparable piezoelectric properties with an output current of up to 15 nA and output voltage of up to 0.6 V under pressure stimulation, remaining stable after 200 cycles of pressure release without significant decay. Meanwhile, the mechanical properties of the LN/CNTs/SF-nanofiber scaffolds (SF-NFSs) are also enhanced, with a tensile strength reaching 12.84 MPa and an elongation at break reaching 80.07%. Importantly, in vitro cell proliferation experiments showed that the LN/CNTs/SF-NFSs promoted cell proliferation at a rate of 43%. Accordingly, the mouse wound healing experiments further indicated that they could accelerate the healing of skin wounds in mice that were continuously moving. Therefore, SF-based piezoelectric nanofibrous scaffolds exhibit potential for use in rapid wound healing and this sheds light on smart treatment for tissue engineering in biomedicine.


Asunto(s)
Fibroínas , Nanofibras , Ratones , Animales , Andamios del Tejido , Cicatrización de Heridas , Ingeniería de Tejidos , Seda
4.
Anal Chem ; 95(12): 5307-5315, 2023 03 28.
Artículo en Inglés | MEDLINE | ID: mdl-36930830

RESUMEN

The rarity of circulating tumor cells (CTCs) and the complexity of blood components present major challenges for the efficient isolation of CTCs in blood. The coexisting matters could interfere with the detection of CTCs by adhering to the binding sites on the material surface, leading to the reduced accuracy of biomarker capture in blood. Herein, we developed dynamic bioactive lubricant-infused slippery surfaces by grafting the 1H,1H,2H,2H-heptadecafluorodecyl acrylate polymer and 3-acrylamidophenylboronic acid polymer brushes on quartz plates by UV light-initiated and then grafted cancer cell-binding peptides via reversible catechol-boronate chemistry between phenylboronic acid groups and 3,4-dihydroxy-l-phenylalanine groups of peptides for high-efficient capture of CTCs and nondestructive release of the desired cells in sugar response. Patterned dynamic bioactive lubricant-infused surfaces (PDBLISs) further exhibited the improved capture efficiency of CTCs and more effective antifouling properties for nonspecific cells and blood components. Moreover, the PDBLIS can efficiently capture rare cancer cells from the mimic of cancer patient's blood samples. We anticipate that the strategy we proposed would be used in further clinical diagnosis of complicated biofluids related to a variety of tumors and exhibit good prospects and potential in future liquid biopsies.


Asunto(s)
Células Neoplásicas Circulantes , Humanos , Separación Celular , Células Neoplásicas Circulantes/patología , Células MCF-7 , Línea Celular Tumoral , Péptidos
5.
Int J Biol Macromol ; 239: 124272, 2023 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-37001785

RESUMEN

It is imperative to develop an antibiotic-free and long-term effective strategy for treating chronic wound infections due to the long-term utilization of antibiotics easily causing drug resistance. Herein, we fabricated a novel poly-N-isopropylacrylamide (PNIPAM)/polyacrylamide (PAM) coupling thermosensitive hydrogel integrating 1D lysozyme nanofiber doped with CuS nanoparticles (CuS/PP) and loading antibacterial peptide melittin (M) (CuS/PP-M) for combating chronic wound infection via photothermal modulating the release of melittin. For the CuS/PP-M hydrogel, the copolymerization of PNIPAM and PAM allows the lower critical solution temperature (LCST) higher than the body temperature, effectively hindering the spontaneous release of melittin when contacts the infected wound, while the integration of LNF/CuS nanofibers provides a stable photothermal treatment for triggering the release of melittin. As a result, the CuS/PP-M hydrogel exhibits synergistically enhanced effect on killing both Gram-positive and Gram-negative bacteria, which maintains more than 99 % bactericidal efficiency, even displays a long-term and multiply antibacterial performance by photothermal modulating melittin release. Moreover, the CuS/PP-M hydrogel presents both high antibacterial activity and excellent wound healing performance in the mouse wound model, thereby benefiting the chronic wound healing.


Asunto(s)
Antibacterianos , Meliteno , Animales , Ratones , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Meliteno/farmacología , Temperatura , Hidrogeles/uso terapéutico , Bacterias Gramnegativas , Bacterias Grampositivas , Vendajes
6.
Mater Horiz ; 10(2): 512-523, 2023 02 06.
Artículo en Inglés | MEDLINE | ID: mdl-36416286

RESUMEN

The fast monitoring of oral bacterial infection, bacterial clearance and repairing of enamel damage caused by dental caries relies on an effective way of monitoring, killing and repairing in situ, but presents a major challenge in oral healthcare. Herein, we developed a bio-inspired versatile free-standing membrane by filling TiO2 nanotube arrays with ß-sheet-rich silk fibroin and cleaving them from Ti foil, as inspired by nacre or enamel-like structures. The robust transparent membrane exhibited good mechanical properties, and could indicate acid-base microenvironment variation and the infection of S. mutans in a 5 min test by loading cyanidin cations in the membrane. Meanwhile, it can be used for photocatalysis and nanoreservoirs ascribed to TiO2 nanotubes, to kill and remove 99% of S. mutans bacteria under interval UV irradiation with low-power density, and load functional peptide to induce the remineralization on the etched-enamel for long-term treatment, tested in vitro and in vivo. The mechanical property of repaired enamel is improved in comparison. This bio-inspired constructed membrane would be applied in the prevention and treatment of oral cavity related diseases, such as enamel demineralization and dental caries, etc.


Asunto(s)
Caries Dental , Humanos , Caries Dental/prevención & control , Remineralización Dental , Boca , Bacterias
7.
Langmuir ; 38(40): 12346-12355, 2022 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-36173231

RESUMEN

With the revelation of the close link between Alzheimer's disease (AD) and type II diabetes (T2D) and the possible assembly of multiple amyloid peptides therein, it is critical to understand and regulate the co-fibrillation pathway between related amyloid peptides. Here, we show experimentally and theoretically that electric field (EF) inhibited hybrid amyloid fibrillation of ß-amyloid peptide (Aß) and human islet amyloid peptide (hIAPP) by modulating the hetero-aggregation pathway. Experimental results confirm that the ß-sheet secondary structure of amyloid peptides would be disrupted under small static EF and accompanied by transforming fibril aggregates into amorphous particles in vitro. Molecular dynamics simulations further demonstrate that even with the transformation of the secondary structure from ß-sheet to random coil, the strong interaction between Aß and hIAPP peptides would remain largely unaffected under the small static EF, leading to the formation of amorphous nanoparticles observed in the experiments. This inhibitory effect of EF on the co-fibrillation of multiple amyloid peptides might contribute to reducing the mutual deterioration of different degenerative diseases and show great potential for the noninvasive treatment of amyloid-related diseases.


Asunto(s)
Diabetes Mellitus Tipo 2 , Polipéptido Amiloide de los Islotes Pancreáticos , Amiloide , Péptidos beta-Amiloides/química , Proteínas Amiloidogénicas , Diabetes Mellitus Tipo 2/complicaciones , Diabetes Mellitus Tipo 2/metabolismo , Humanos , Polipéptido Amiloide de los Islotes Pancreáticos/química , Simulación de Dinámica Molecular
8.
Front Neurorobot ; 16: 925210, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35874108

RESUMEN

Urban transportation destination prediction is a crucial issue in the area of intelligent transportation, such as urban traffic planning and traffic congestion control. The spatial structure of the road network has high nonlinearity and complexity, and also, the traffic flow is dynamic due to the continuous changing of the traffic environment. Thus, it is very important to model the spatial relation and temporal dependence simultaneously to simulate the true traffic conditions. Most of the existing destination prediction methods have limited ability to model large-scale spatial data that changes dynamically with time, so they cannot obtain satisfactory prediction results. This paper proposes a human-in-loop Spatial-Temporal Attention Mechanism with Graph Convolutional Network (STAGCN) model to explore the spatial-temporal dependencies for destination prediction. The main contributions of this study are as follows. First, the traffic network is represented as a graph network by grid region dividing, then the spatial-temporal correlations of the traffic network can be learned by convolution operations in time on the graph network. Second, the attention mechanism is exploited for the analysis of features with loop periodicity and enhancing the features of key nodes in the grid. Finally, the spatial and temporal features are combined as the input of the Long-Short Term Memory network (LSTM) to further capture the spatial-temporal dependences of the traffic data to reach more accurate results. Extensive experiments conducted on the large scale urban real dataset show that the proposed STAGCN model has achieved better performance in urban car-hailing destination prediction compared with the traditional baseline models.

9.
Cells ; 11(11)2022 05 25.
Artículo en Inglés | MEDLINE | ID: mdl-35681433

RESUMEN

Herein, we have verified the interaction between the functional peptides from the SARS-CoV-2 and cell membrane, and we further proved that peptides exhibit little membrane disruption. The specific amino acids (Lys, Ile, Glu, Asn, Gln, etc.) with charge or hydrophobic residues play a significant role during the functional-peptide binding to membrane. The findings could provide the hints related to viral infection and also might pave the way for development of new materials based on peptides with membrane-binding activity, which would enable functional peptides further as peptide adjuvants, in order to help deliver the cancer drug into tumor cells for the efficient tumor therapy.


Asunto(s)
COVID-19 , Simulación de Dinámica Molecular , Secuencia de Aminoácidos , Membrana Celular/metabolismo , Humanos , Péptidos/metabolismo , SARS-CoV-2 , Tripsina/metabolismo
10.
J Colloid Interface Sci ; 614: 677-689, 2022 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-35124295

RESUMEN

The enhancement of the self-cleaning ability of photocatalytic membranes and their degradation efficiency over tetracycline (TC) still remains a challenge. In this study, an alternative silver vanadate quantum dots (AgVO3 QDs) doped reduced graphene oxide (RGO) and graphitic carbon nitride (C3N4) nanocomposites modified polyvinylidene fluoride (PVDF) membrane (AgVO3/RGO/C3N4-PVDF) was successfully fabricated to enhance the photocatalytic activity. The AgVO3/RGO/C3N4 nanocomposites were functioned as the active component for the photocatalytic membrane. The unique Z-scheme heterostructure of AgVO3/RGO/C3N4 and the porous PVDF framework synergistically enhanced the separation and transport efficiency of photogenerated carriers and facilitated the interaction between the photocatalyst and the pollutant. As a result, the degradation efficiency of TC for the AgVO3/RGO/C3N4-PVDF reached 88.53% within 120 min, which was higher than those of the binary component membranes (64.8% for RGO/C3N4-PVDF and 79.18% AgVO3/C3N4-PVDF). In addition, AgVO3/RGO/C3N4-PVDF exhibited high permeability (1977 L·m-2·h-1·bar-1) and excellent antifouling activity. Under visible-light irradiation, the flux recovery rate (FRR) increased from 92.4% to 99.1%. Furthermore, AgVO3/RGO/C3N4-PVDF could reject 97.4% of Escherichia coli (E. coli) owning to its self-cleaning capacity, and eliminated the E. coli under visible-light irradiation trough the photogeneration of h+. This study highlights a highly efficient photocatalytic membrane based on a Z-scheme heterostructure, which may have a great potential application in practical wastewater treatment.


Asunto(s)
Puntos Cuánticos , Catálisis , Escherichia coli , Polímeros de Fluorocarbono , Grafito , Compuestos de Nitrógeno , Polivinilos , Compuestos de Plata , Vanadatos
11.
J Colloid Interface Sci ; 593: 142-151, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-33744525

RESUMEN

The scaffold materials with good mechanical and structural properties, controlled drug release performance, biocompatibility and biodegradability are important tenet in tissue engineering. In this work, the functional core-shell nanofibers with poly(ε-caprolactone) (PCL) as shell and silk fibroin heavy chain (H-fibroin) as core were constructed by emulsion electrospinning. The transmission electron microscopy confirmed that the nanofiber with core-shell structure were successfully prepared. The constructed nanofiber materials were characterized by the several characterization methods. The results showed that ethanol treatment could induce the formation of ß-sheet of H-fibroin in composite nanofibers, thus improving the mechanical properties of PCL/H-fibroin nanofiber scaffold. In addition, we evaluated the potential of PCL/H-fibroin nanofiber membrane as a biological scaffold. It was found that PCL/H-fibroin nanofiber scaffold was more conducive to cell adhesion and proliferation with the increment of H-fibroin. Finally, in vitro drug release presented that PCL/H-fibroin core-shell nanofibers could effectively reduce the prophase burst of drug molecules and show the sustained drug release. The PCL/H-fibroin nanofiber scaffolds constructed in this work have good mechanical properties, biocompatibility, and display good potential in biomedical applications, such as drug carriers, tissue engineering and wound dressings, etc.


Asunto(s)
Fibroínas , Nanofibras , Liberación de Fármacos , Poliésteres , Seda , Ingeniería de Tejidos , Andamios del Tejido
12.
J Colloid Interface Sci ; 586: 335-348, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33190831

RESUMEN

Designing photocatalytic membranes with excellent photocatalytic and self-cleaning ability based on the synergistic effect between the crystal structure of membrane matrix and photocatalyst is highly desirable. Herein, Z-scheme 2D/3D g-C3N4/BiOI heterojunction blended in beta-phase polyvinylidene fluoride membrane (ß-phase PVDF) was prepared via solvent crystallization and phase inversion technique. As expected, the designed g-C3N4/BiOI/ß-phase PVDF photocatalytic membranes (CN/BI/ß-phase PVDF PMs) achieved exceptional photocatalytic degradation efficiency for tetracycline (94.6%) as compared to the CN/BI heterojunction power (84.0%) and two other control membrane matrixes (CN/BI/PAN and CN/BI/CA PMs) within 120 min. Meanwhile, the dynamic cyclic degradation system of CN/BI/ß-phase PVDF PMs was also investigated that reached to be 94.8% in 80 min. Besides, the CN/BI/ß-phase PVDF PMs not only had outstanding self-cleaning activity and remarkable permeability (up to 30,688 L·m-2·h-1) but also had high stability and reusability even after five runs. Importantly, the hydroxyl radical detection and ESR analysis identified that the ß-phase PVDF membrane could promote photoinduced carrier separation efficiency of 2D/3D g-C3N4/BiOI heterojunction. This work may open up a novel strategy for designing and constructing high-efficient photocatalytic membranes for water treatment.


Asunto(s)
Luz , Tetraciclina , Catálisis , Fotólisis , Polivinilos , Porosidad
13.
ACS Appl Bio Mater ; 3(6): 3648-3655, 2020 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-35025235

RESUMEN

Membrane-disrupting antimicrobial peptides continue to attract increasing attention due to their potential to combat multidrug-resistant bacteria. However, some limitations are found in the success of clinical setting-based antimicrobial peptide agents, for instance, the poor stability of antimicrobial peptides in vivo and their short-term activity. Self-assembled peptide materials can improve the stability of antimicrobial peptides, but the biosafety of peptide-based materials is the main concern, although they are considered to be biocompatible, because some peptide aggregates would possibly induce protein misfolding, which could be related to amyloid-related diseases. Therefore, in this work, we designed two peptides and constructed peptide-based nanofibrils by self-assembly before its utilization. It is found that the fibrils could release the antimicrobial peptide by disassembly for microbial membrane lysis in the presence of bacteria. The designed peptide-based fibrils presented a good and long-term antimicrobial activity with bacterial membrane disruption and the efflux of calcium from bacteria. Furthermore, it could be used to construct hybrid macrofilms displaying low cytotoxicity, low hemolytic activity, and good biocompatibility. The innovative design strategy could be beneficial for the development of smart antimicrobial nanomaterials.

14.
Spectrochim Acta A Mol Biomol Spectrosc ; 228: 117845, 2020 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-31784226

RESUMEN

A novel quinazolinone based turn-on fluorescence probe for sensitive monitoring hypochlorite was prepared using the mild condensation reaction between 2-(2'-hydroxyphenyl)-4(3H)-quinazolinone derivative and 4-methylbenzenesulfonyl hydrazide. The probe exhibited specific selectivity to ClO- with obvious optical signal changes from weak fluorescence at 560 nm to a strong fluorescence emission at 520 nm and color changes from colorless to yellow, which could be noticed by the naked eye. The detection limit toward hypochlorite is as low as 11.4 nM. Moreover, the probe could sensitively response to ClO- in living cells with satisfying imaging effect and has been successfully applied to the determination of ClO- in practical water samples, which indicated that the probe has certain application potential for hypochlorite monitoring.


Asunto(s)
Colorantes Fluorescentes/química , Ácido Hipocloroso/análisis , Quinazolinonas/química , Espectrometría de Fluorescencia/instrumentación , Isótopos de Carbono , Línea Celular Tumoral , Cumarinas , Humanos , Límite de Detección , Espectroscopía de Resonancia Magnética , Imagen Óptica , Reproducibilidad de los Resultados , Espectrometría de Fluorescencia/métodos , Espectrofotometría Ultravioleta
15.
Colloids Surf B Biointerfaces ; 181: 341-348, 2019 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-31158696

RESUMEN

Alzheimer's disease (AD) is a common neurodegenerative disease characterized by cognitive and memory function impairment. Studies have shown that the overproduction and further misfolding of amyloid polypeptide (Aß) are the main causes of AD. Therefore, how to reduce Aß species, especially to disassemble the preformed amyloid aggregates, has become the focus of related research. Photodynamic therapy (PDT) using normal photosensitizers eg. porphyrins, is a traditional way of inhibiting amyloid aggregation or degrading the amyloid aggregates, but UV light irradiation presents a side effect for the damage of normal tissue, which limits its medical application. Upconversion nanoparticles (UCNPs) is a luminous material which can be modified by photodynamic agent to form the complex generating reactive oxygen species (ROS) with noninvasive light irradiation. It presents a good advantage in the disassembly of amyloid aggregates via ROS in noninvasive light irradiation. Herein, we prepared ß-NaYF4:Yb/Er@SiO2@RB by combing UCNPs with photosensitizer to disassemble preformed Aß aggregates under near-infrared light. The results displayed upconversion nanomaterials could degrade Aß1-42 fibrils effectively by photo-generating ROS, which demonstrated that the functional UCNPs may have potential applications in the treatment of amyloid diseases in future.


Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Amiloide/antagonistas & inhibidores , Rayos Infrarrojos , Nanopartículas/química , Fotoquimioterapia , Fármacos Fotosensibilizantes/farmacología , Enfermedad de Alzheimer/metabolismo , Amiloide/química , Amiloide/metabolismo , Humanos , Tamaño de la Partícula , Agregado de Proteínas/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Propiedades de Superficie
16.
PLoS One ; 13(7): e0200169, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29979778

RESUMEN

Estimation of remaining capacity is essential for ensuring the safety and reliability of lithium-ion batteries. In actual operation, batteries are seldom fully discharged. For a constant current-constant voltage charging mode, the incomplete discharging process affects not only the initial state but also processed variables of the subsequent charging profile, thereby mainly limiting the applications of many feature-based capacity estimation methods which rely on a whole cycling process. Since the charging information of the constant voltage profile can be completely saved whether the battery is fully discharged or not, a geometrical feature of the constant voltage charging profile is extracted to be a new aging feature of lithium-ion batteries under the incomplete discharging situation in this work. By introducing the quantum computing theory into the classical machine learning technique, an integrated quantum particle swarm optimization-based support vector regression estimation framework, as well as its application to characterize the relationship between extracted feature and battery remaining capacity, are presented and illustrated in detail. With the lithium-ion battery data provided by NASA, experiment and comparison results demonstrate the effectiveness, accuracy, and superiority of the proposed battery capacity estimation framework for the not entirely discharged condition.


Asunto(s)
Suministros de Energía Eléctrica , Litio , Suministros de Energía Eléctrica/estadística & datos numéricos , Electricidad , Electroquímica , Iones , Reproducibilidad de los Resultados , Máquina de Vectores de Soporte
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