Green analytical assay for the viability assessment of single maize seeds using double-threshold strategy for catalase activity and malondialdehyde content.

The development of nondestructive technology for the detection of seed viability is challenging. In this study, to establish a green and effective method for the viability assessment of single maize seeds, a two-stage seed viability detection method was proposed. The catalase (CAT) activity and malondialdehyde (MDA) content were selected as the most key biochemical components affecting maize seed viability, and regression prediction models were developed based on their hyperspectral information and a data fusion strategy. Qualitative discrimination models for seed viability evaluation were constructed based on the predicted response values of the selected key biochemical components. The results showed that the double components thresholds strategy achieved the highest discrimination accuracy (92.9%), providing a crucial approach for the rapid and environmentally friendly detection of seed viability.

Non-destructive detection of single-seed viability in maize using hyperspectral imaging technology and multi-scale 3D convolutional neural network.

The viability of Zea mays seed plays a critical role in determining the yield of corn. Therefore, developing a fast and non-destructive method is essential for rapid and large-scale seed viability detection and is of great significance for agriculture, breeding, and germplasm preservation. In this study, hyperspectral imaging (HSI) technology was used to obtain images and spectral information of maize seeds with different aging stages. To reduce data input and improve model detection speed while obtaining more stable prediction results, successive projections algorithm (SPA) was used to extract key wavelengths that characterize seed viability, then key wavelength images of maize seed were divided into small blocks with 5 pixels ×5 pixels and fed into a multi-scale 3D convolutional neural network (3DCNN) for further optimizing the discrimination possibility of single-seed viability. The final discriminant result of single-seed viability was determined by comprehensively evaluating the result of all small blocks belonging to the same seed with the voting algorithm. The results showed that the multi-scale 3DCNN model achieved an accuracy of 90.67% for the discrimination of single-seed viability on the test set. Furthermore, an effort to reduce labor and avoid the misclassification caused by human subjective factors, a YOLOv7 model and a Mask R-CNN model were constructed respectively for germination judgment and bud length detection in this study, the result showed that mean average precision (mAP) of YOLOv7 model could reach 99.7%, and the determination coefficient of Mask R-CNN model was 0.98. Overall, this study provided a feasible solution for detecting maize seed viability using HSI technology and multi-scale 3DCNN, which was crucial for large-scale screening of viable seeds. This study provided theoretical support for improving planting quality and crop yield.

Monitoring the major taste components during black tea fermentation using multielement fusion information in decision level.

Hitherto, the intelligent detection of black tea fermentation quality is still a thought-provoking problem because of one-side sample information and poor model performance. This study proposed a novel method for the prediction of major chemical components including total catechins, soluble sugar and caffeine using hyperspectral imaging technology and electrical properties. The multielement fusion information were used to establish quantitative prediction models. The performance of model using multielement fusion information was better than that of model using single information. Subsequently, the stacking combination model using fusion data combined with feature selection algorithms for evaluating the fermentation quality of black tea. Our proposed strategy achieved better performance than classical linear and nonlinear algorithms, with the correlation coefficient of the prediction set (Rp) for total catechins, soluble sugar and caffeine being 0.9978, 0.9973 and 0.9560, respectively. The results demonstrated that our proposed strategy could effectively evaluate the fermentation quality of black tea.

Qualitative and quantitative detection of aflatoxins B1 in maize kernels with fluorescence hyperspectral imaging based on the combination method of boosting and stacking.

The most widespread, toxic, and harmful toxin is aflatoxins B1 (AFB1). The fluorescence hyperspectral imaging (HSI) system was employed for AFB1 detection in this study. This study developed the under sampling stacking (USS) algorithm for imbalanced data. The results indicated that the USS method combined with ANOVA for featured wavelength achieved the best performance with the accuracy of 0.98 for 20 or 50 µg /kg threshold using endosperm side spectra. As for the quantitative analysis, a specified function was used to compress AFB1 content, and the combination of boosting and stacking was used for regression. The support vector regression (SVR)-Boosting, Adaptive Boosting (AdaBoost), and extremely randomized trees (Extra-Trees)-Boosting were used as the base learner, while the K nearest neighbors (KNN) algorithm was used as the meta learner could obtain the best results, with the correlation coefficient of prediction (Rp) was 0.86. These results provided the basis for developing AFB1 detection and estimation technologies.

Rapid and Non-destructive Classification of New and Aged Maize Seeds Using Hyperspectral Image and Chemometric Methods.

The aged seeds have a significant influence on seed vigor and corn growth. Therefore, it is vital for the planting industry to identify aged seeds. In this study, hyperspectral reflectance imaging (1,000-2,000 nm) was employed for identifying aged maize seeds using seeds harvested in different years. The average spectra of the embryo side, endosperm side, and both sides were extracted. The support vector machine (SVM) algorithm was used to develop classification models based on full spectra to evaluate the potential of hyperspectral imaging for maize seed detection and using the principal component analysis (PCA) and ANOVA to reduce data dimensionality and extract feature wavelengths. The classification models achieved perfect performance using full spectra with an accuracy of 100% for the prediction set. The performance of models established with the first three principal components was similar to full spectrum models, but that of PCA loading models was worse. Compared to other spectra, the two-band ratio (1,987 nm/1,079 nm) selected by ANOVA from embryo-side spectra achieved a better classification accuracy of 95% for the prediction set. The image texture features, including histogram statistics (HS) and gray-level co-occurrence matrix (GLCM), were extracted from the two-band ratio image to establish fusion models. The results demonstrated that the two-band ratio selected from embryo-side spectra combined with image texture features achieved the classification of maize seeds harvested in different years with an accuracy of 97.5% for the prediction set. The overall results indicated that combining the two wavelengths with image texture features could detect aged maize seeds effectively. The proposed method was conducive to the development of multi-spectral detection equipment.

Application of long-wave near infrared hyperspectral imaging for determination of moisture content of single maize seed.

Moisture content (MC) is one of the most important factors for assessment of seed quality. However, the accurate detection of MC in single seed is very difficult. In this study, single maize seed was used as research object. A long-wave near infrared (LWNIR) hyperspectral imaging system was developed for acquiring reflectance images of the embryo and endosperm side of maize seed in the spectral range of 930-2548 nm, and the mixed spectra were extracted from both side of maize seeds. Then, Full-spectrum models were established and compared based on different types of spectra. It showed that models established based on spectra of the embryo side and mixed spectra obtained better performance than the endosperm side. Next, a combination of competitive adaptive reweighted sampling (CARS) and successive projections algorithm (SPA) was proposed to select the most effective wavelengths from full-spectrum data. In order to explore the stableness of wavelength selection algorithm, these methods were used for 200 independent experiments based on embryo side and mixed spectra, respectively. Each selection result was used as input of partial least squares regression (PLSR) and least squares support vector machine (LS-SVM) to build calibration models for determining the MC of single maize seed. Results indicated that the CARS-SPA-LS-SVM model established with mixed spectra was optimal for MC prediction in all models by considering the accuracy, stableness and complexity of models. The prediction accuracy of CARS-SPA-LS-SVM model is Rpre = 0.9311 ± 0.0094 and RMSEP = 1.2131 ± 0.0702 in 200 independent assessment. The overall study revealed that the long-wave near infrared hyperspectral imaging can be used to non-invasively and fast measure the MC in single maize seed and a robust and accurate model could be established based on CARS-SPA-LS-SVM method coupled with mixed spectral. These results can provide a useful reference for assessment of other internal quality attributes (such as starch content) of single maize seed.

Nondestructive evaluation of soluble solids content in tomato with different stage by using Vis/NIR technology and multivariate algorithms.

In this study Vis/NIR spectroscopy was applied to evaluate soluble solids content (SSC) of tomato. A total of 168 tomato samples with five different maturity stages, were measured by two developed systems with the wavelength ranges of 500-930 nm and 900-1400 nm, respectively. The raw spectral data were pre-processed by first derivative and standard normal variate (SNV), respectively, and then the effective wavelengths were selected using competitive adaptive reweighted sampling (CARS) and random frog (RF). Partial least squares (PLS) and least square-support vector machines (LS-SVM) were employed to build the prediction models to evaluate SSC in tomatoes. The prediction results revealed that the best performance was obtained using the PLS model with the optimal wavelengths selected by CARS in the range of 900-1400 nm (Rp = 0.820 and RMSEP = 0.207 °Brix). Meanwhile, this best model yielded desirable results with Rp and RMSEP of 0.830 and 0.316 °Brix, respectively, in 60 samples of the independent set. The method proposed from this study can provide an effective and quick way to predict SSC in tomato.

An on-bacterium flow cytometric immunoassay for protein quantification.

The polystyrene bead-based flow cytometric immunoassay has been widely reported. However, the preparation of functional polystyrene bead is still inconvenient. This study describes a simple and easy on-bacterium flow cytometric immunoassay for protein quantification, in which Staphylococcus aureus (SAC) is used as an antibody-antigen carrier to replace the polystyrene bead. The SAC beads were prepared by carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling, paraformaldehyde fixation and antibody binding. Carcinoembryonic antigen (CEA) and cytokeratin-19 fragment (CYFRA 21-1) proteins were used as models in the test system. Using prepared SAC beads, biotinylated proteins, and streptavidin-phycoerythrin (SA-PE), the on-bacterium flow cytometric immunoassay was validated by quantifying CEA and CYFRA 21-1 in sample. Obtained data demonstrated a concordant result between the logarithm of the protein concentration and the logarithm of the PE mean fluorescence intensity (MFI). The limit of detection (LOD) in this immunoassay was at least 0.25 ng/ml. Precision and accuracy assessments appeared that either the relative standard deviation (R.S.D.) or the relative error (R.E.) was <10%. The comparison between this immunoassay and a polystyrene bead-based flow cytometric immunoassay showed a correlation coefficient of 0.998 for serum CEA or 0.996 for serum CYFRA 21-1. In conclusion, the on-bacterium flow cytometric immunoassay may be of use in the quantification of serum protein.