Supplementation with protected kapok seed oil and choline chloride to improve the performance and lipid status of thin-tailed sheep.

Background and Aim: Healthy meat production is an important aspect of increasing sheep productivity. This study aimed to examine the influence of protected kapok seed oil (KSO) in combination with choline chloride (CC) on the feed utilization, lipid status, and performance of thin-tailed sheep. Materials and Methods: Thirty male thin-tailed sheep (approximately 6 months old, with an average body weight of 12.59 ± 1.48 kg) were divided into six treatment groups (five heads/treatment). Factor 1 consisted of two treatments: K1 (KSO supplementation at 10% supplementation and 75% protection level) and K0 (without KSO supplementation). Factor 2 consisted of three levels of CC: (C0: 0%; C1: 1.5% and C2: 3% feed dry matter (DM) basis supplementation levels). The variables measured were the DM consumption, DM digestibility, organic matter digestibility, nitrogen retention, daily body weight gain (DBWG), and blood and meat lipid status. The data were analyzed using analysis of variance in a completely randomized design in a factorial pattern of 2 × 3 × 5. Results: Choline chloride supplementation (up to 3%) increased DM consumption in the K0C2 group. The CC and protected KSO (K1C2) supplementation combination resulted in the highest DM consumption level (p < 0.05). The protected KSO supplementation increased DBWG (the DBWG in the K1C0 group was higher than that in the K0C0 group, and the highest DBWG was found in the K1C2 group) (p < 0.05). Protected KSO and CC supplementation decreased cholesterol levels and increased the relative proportion of linoleic acid in meat (p < 0.05). Conclusion: Combined supplementation with protected KSO and CC improved the feed utilization and performance of male thin-tailed sheep. There were increases in DBWG, decreases in intramuscular fat and cholesterol levels, and increases in meat linoleic acid levels.

Gut Microbiota Profiles in Dairy Cattle from Highland and Coastal Regions Using Shotgun Metagenomic Approach.

There is significant difference in milk production of highland and coastal regions in Indonesia of which the latter is critically low. The recent studies indicate a possibility of improving the milk yield and quality by manipulating the gut microbiota, for which profiling and abundance of gut microbiota in these divergent regions need to be addressed. The present study was the first of its kind to explore the dairy cattle gut microbiota diversity, abundance, and functional annotation of the two divergent Indonesian regions, the highland and coastal regions, by shotgun metagenomic approach. Unfavorable environmental conditions such as type of forage grass in coastal regions and high temperature remain a limiting factor; however, the improvement through manipulating the gut microbiota was not considered until recently to improve the quality and quantity of coastal region dairy cattle. The application of recent advance technologies can help achieve this goal on sustainable basis. The results show Bacteroidetes in higher abundance in coastal region (FPP) than in highland (Salatiga) while Firmicutes were higher in Salatiga. Furthermore, a collective physiology of the community was found by annotating the sequences against KEGG, eggNOG, and CAZy databases. To identify the role in pathways, an mPATH analysis was performed to have insight into the microbiota community in different metabolic pathways. The identified targets can be used as prebiotic and/or probiotic to improve the average milk yield of coastal region dairy cattle by manipulating the dairy feed with desired microbes.

Effect of feeding goat meat containing low cholesterol and rich omega-6 fatty acid on blood lipid status of white rat (Rattus norvegicus).

BACKGROUND AND AIM: Healthy goat meat is an essential aspect in increasing consumer acceptance for this livestock product. The research aimed to examine the effect of goat meat containing low cholesterol and rich omega-6 fatty acid on the performance and blood lipid status of white rats (Rattus norvegicus). MATERIALS AND METHODS: Thirty 2-month-old male white rats (R. norvegicus) weighing 195-230 g were randomly divided into three groups, with each group consisting of 10 rats. Group I was treated with a control feed (T0; BR I concentrate). Group II (T1) was treated with a mixed feed containing 50% control feed and 50% goat meat. Group III (T2) was treated with a mixed feed comprising 50% control feed and 50% goat meat with low cholesterol and rich omega-6 fatty acids. Each treatment was given ad libitum for 30 days. The variables measured were dry matter and organic matter consumption, daily body weight gain, feed conversion, triglyceride levels, total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, and atherogenic index (AI). The data were analyzed statistically using analysis of variance in a completely randomized design. RESULTS: The total, HDL, and LDL cholesterol levels at T0, T1, and T2 were as follows: 99.97, 35.97, and 50.43 mg/dL (total cholesterol); 108.35, 33.92, and 58.17 mg/dL (HDL cholesterol); and 101.43, 38.09, and 48.65 mg/dL (LDL cholesterol). The highest HDL and the lowest LDL cholesterol levels (p<0.05) were observed in the T2 treatment group, which had the lowest AI (1.69 vs. 1.77 and 2.19). CONCLUSION: The consumption of goat with low cholesterol and rich omega-6 fatty acids reduces the total cholesterol and LDL cholesterol, raises the HDL cholesterol levels, and decreases the AI.

Effect of KOROPASS, an extruded jack bean (Canavalia ensiformis)-derived supplement, on productivity and economic performance of beef cattle.

AIM: This study evaluated the effect of feeding a graded amount of extruded jack bean (Canavalia ensiformis) on nutritional status, production performances, and economic performance of beef cattle. MATERIALS AND METHODS: The supplement called "KOROPASS" was prepared from the extruded jack bean (according to the extrusion heating process). Sixteen male Friesian-Holstein crossbred cattle were divided into four groups and fed on KOROPASS as per the regimen: R0 (total mixed ration [TMR] without KOROPASS), R1 (TMR supplemented with 3% KOROPASS), R2 (TMR supplemented with 6% KOROPASS), and R3 (TMR supplemented with 9% KOROPASS). The in vivo experiment lasted 44 days. TMR contained 12% crude protein and 60% total digestible nutrient. The consumption and digestibility of dry matter (DM), organic matter (OM), and total protein (TP), feed efficiency, average daily gain, and income over feed cost (IOFC) were evaluated. RESULTS: KOROPASS supplementation significantly increased (p<0.05) beef cattle consumption of DM (from 7.83 [R0] to 8.33 [R1], 8.91 [R2], and 9.69 kg/day [R3]), OM (from 6.72 to 7.17, 7.69, and 8.38 kg/day, respectively), and TP (from 892 to 1020, 1182, and 1406 g/day, respectively). The elevated levels of KOROPASS significantly increased (p<0.05) digestibility in terms of the levels of DM (from 42.9 [R0] to 50.6 [R1], 58.0 [R2], and 63.6% [R3]), OM (from 54.3 to 59.6, 66.3, and 70.6%, respectively), and TP (from 65.0 to 67.1, 75.0, and 80.7%, respectively). Dietary supplementation of KOROPASS significantly increased (p<0.05) metabolizable protein, average daily weight gain, and feed efficiency of beef cattle. Finally, dietary KOROPASS supplementation, especially at 9%, resulted in the highest (p<0.05) IOFC value of beef cattle. CONCLUSION: Dietary supplementation of KOROPASS improved feed utility, as reflected by the increase in consumption and digestibility of DM, OM, and TP. Further, KOROPASS supplementation improved feed efficiency, growth, and economic performance of beef cattle. The findings indicate the potential value of KOROPASS as a feed supplement for beef cattle.