Water sector resilience in the United Kingdom and Ireland: The COVID-19 challenge.

The outbreak of COVID-19 led to restrictions on movements and activities, which presented a serious challenge to the resilience of the water sector. It is essential to understand how successfully water companies responded to this unprecedented event so effective plans can be built for future disruptive events. This study aimed to evaluate how the water sectors in the UK and Ireland were affected from a holistic sustainability and resilience-based perspective. Using pre-COVID data for 18 indicators of company performance and comparing them to the first year of the pandemic, the direction and magnitudes of change varied across companies. Financial indicators were significantly negatively affected, with interest cover ratio, post-tax return on regulated equity, and operating profit, exhibiting the greatest average declines of 21%, 21%, and 18%, respectively, a trend that would be dangerous to provisions and company operations if continued. Despite this, service and environmental indicators improved during the first year of the pandemic, exemplified by unplanned outage, risk of sewer storm flooding, and water quality compliance risk decreasing by a mean average of 37%, 32%, and 27%, respectively. Analysis using the Hicks-Moorsteen Productivity Index concluded that average productivity increased by 35%. The results suggest that the water sector was relatively resilient to the COVID-19 pandemic in terms of services, but adverse effects may have manifested in a deteriorated financial position that could exacerbate future challenges arising from exogenous pressures such as climate change. Specific advice for the UK water sector is to scrutinize non-critical spending, such as shareholder payments, during periods of economic downturn to ensure essential capital projects can be carried out. Although results are temporal and indicator selection sensitive, we recommend that policy, regulation, and corporate culture embrace frameworks that support long-term resilience to since the relative success in response to COVID-19 does not guarantee future success against differing challenges. This study generates a timely yet tentative insight into the diverse performance of the water sector during the pandemic, pertinent to the water industry, regulators, academia, and the public.

Heat recovery and water reuse in micro-distilleries improves eco-efficiency of alcohol production.

The number of micro-scale spirit distilleries worldwide has grown considerably over the past decade. With an onus on the distillery sector to reduce its environmental impact, such as carbon emissions, opportunities for increasing energy efficiency need to be implemented. This study explores the potential environmental benefits and financial gains achievable through heat recovery from different process and by-product streams, exemplified for a Scotch whisky distillery, but transferrable to micro-distilleries worldwide. The eco-efficiency methodology is applied, taking into account both climate change and water scarcity impacts as well as economic performance of alcohol production with and without heat recovery. A Life Cycle Assessment, focusing on climate change and water scarcity, is combined with a financial assessment considering investment costs and the present value of the savings over the 20-year service life of the heat recovery system. The proposed heat recovery systems allow carbon emission reductions of 8-23% and water scarcity savings of 13-55% for energy and water provision for 1 L of pure alcohol (LPA). Financial savings are comparatively smaller, at 5-13%, due to discounting of the future savings - but offer a simple payback of the investment costs in under two years. The eco-efficiency of the distillery operations can be improved through all proposed heat recovery configurations, but best results are obtained when heat is recovered from mashing, distillations and by-products altogether. A sensitivity analysis confirmed that the methodology applied here delivers robust results and can help guide other micro-distilleries on whether to invest in heat recovery systems, and/or the heat recovery configuration. Uptake should be enhanced through increased information and planning support, and in cases where the distillery offers insufficient heat and water sinks to use all pre-warmed water, opportunities to link with a heat sink outside the distillery are encouraged. A 10% reduction in heating fuel use through heat recovery has the potential to save 47 kt of CO2 eq. or £7.4 M per annum in United Kingdom malt whisky production alone, based on current fuel types used and current prices (2021).

Pitfalls in international benchmarking of energy intensity across wastewater treatment utilities.

The collection, treatment and disposal of wastewater is estimated to consume more than 2% of the world's electrical energy, whilst some wastewater treatment plants (WWTPs) can account for over 20% of electrical consumption within municipalities. To investigate areas to improve wastewater treatment, international benchmarking on energy (electrical) intensity was conducted with the indicator kWh/m3 and a quality control of secondary treatment or better for ≥95% of treated volume. The core sample included 321 companies from 31 countries, however, to analyse regional differences, 11 countries from an external sample made up of various studies of WWTPs was also used in places. The sample displayed a weak-negative size effect with energy intensity, although Kruskal-Wallace analyses showed there was a significant difference between the size of groups (p-value of 0.015), suggesting that as companies get larger; they consume less electricity per cubic metre of wastewater treated. This relationship was not completely linear, as mid to large companies (10,001-100,000 customers) had the largest average consumption of 0.99 kWh/m3. In the regional analysis, EU states had the largest average kWh/m3 with 1.18, which appeared a result of the higher wastewater effluent standards of the region. This was supported by Denmark being the second largest average consuming country (1.35 kWh/m3), since it has some of strictest effluent standards in the world. Along with energy intensity, the associated greenhouse gas (GHG) emissions were calculated enabling the targeting of regions for improvement in response to climate change. Poland had the highest carbon footprint (0.91 kgCO2e/m3) arising from an energy intensity of 0.89 kWh/m3; conversely, a clean electricity grid can affectively mitigate wastewater treatment inefficiencies, exemplified by Norway who emit just 0.013 kgCO2e per cubic meter treated, despite consuming 0.60 kWh/m3. Finally, limitations to available data and the analysis were highlighted from which, it is advised that influent vs. effluent and net energy, as opposed to gross, data be used in future analyses. The large international sample size, energy data with a quality control, GHG analysis, and specific benchmarking recommendations give this study a novelty which could be of use to water industry operators, benchmarking organisations, and regulators.

Elevated serum antibody responses to synthetic mycobacterial lipid antigens among UK farmers: an indication of exposure to environmental mycobacteria?

Background: mycobacterial cells contain complex mixtures of mycolic acid esters. These can be used as antigens recognised by antibodies in the serum of individuals with active tuberculosis, caused by Mycobacterium tuberculosis. In high burden populations, a significant number of false positives are observed; possibly these antigens are also recognised by antibodies generated by other mycobacterial infections, particularly ubiquitous 'environmental mycobacteria'. This suggests similar responses may be observed in a low burden TB population, particularly in groups regularly exposed to mycobacteria. Methods: ELISA using single synthetic trehalose mycolates corresponding to major classes in many mycobacteria was used to detect antibodies in serum of individuals with no known mycobacterial infection, comprising farmers, abattoir workers, and rural and urban populations. Results: serum from four Welsh or Scottish cohorts showed lower (with some antigens significantly lower) median responses than those reported for TB negatives from high-burden TB populations, and significantly lower responses than those with active TB. A small fraction, particularly older farmers, showed strong responses. A second study examined BCG vaccinated and non-vaccinated farmers and non-farmers. Farmers gave significantly higher median responses than non-farmers with three of five antigens, while there was no significant difference between vaccinated or non-vaccinated for either farmer or non-farmer groups. Conclusions: this initial study shows that serodiagnosis with mycobacterial lipid antigens can detect antibodies in a population sub-group that is significantly exposed to mycobacteria, in an assay that is not interfered with by vaccination. Given the links between mycobacterial exposure and a range of immune system diseases, further understanding such responses may provide a new opportunity for monitoring public health and directing treatment.

Impact of Sediment Concentration on the Survival of Wastewater-Derived blaCTX-M-15-Producing E. coli, and the Implications for Dispersal into Estuarine Waters.

The environmental cycling of antibiotic-resistant blaCTX-M-15-producing E. coli following release from wastewater treatment plants is a major public health concern. This study aimed to (i) assess the impact of sediment concentrations on the rate of their inactivation following release from human wastewater into freshwater, and (ii) simulate their subsequent dispersal to the nearby coastline during a "worst-case" event where heavy rainfall coincided with high spring tide in the Conwy Estuary, North Wales. Freshwater microcosms of low, medium and high turbidity were inoculated with blaCTX-M-15 -producing E. coli, then exposed to ultraviolet (UV) radiation. Typical regional wintertime exposure to UV was found to be insufficient to eradicate E. coli, and in highly turbid water, many bacteria survived simulated typical regional summertime UV exposure. Modelling results revealed that blaCTX-M-15-producing E. coli concentrations reduced downstream from the discharge source, with ~ 30% of the source concentration capable of dispersing through the estuary to the coast, taking ~36 h. Offshore, the concentration simulated at key shellfisheries and bathing water sites ranged from 1.4% to 10% of the upstream input, depending on the distance offshore and tidal regime, persisting in the water column for over a week. Our work indicates that the survival of such organisms post-release into freshwater is extended under typical wintertime conditions, which could ultimately have implications for human health.

Emissions down the drain: Balancing life cycle energy and greenhouse gas savings with resource use for heat recovery from kitchen drains.

Although the food service sector is a major user of water, the potential for heat recovery from commercial kitchens' drain water remains largely unexplored. For the first time, we compare the life cycle environmental burdens of producing and installing a heat recovery system with the environmental credits arising from energy savings for a restaurant case study, and for the entire UK food service sector. Life Cycle Assessment was applied to determine the impacts of heat recovery systems made from different materials and comprising a heat exchanger in the shape of a concentric double-walled pipe, pipework and fittings. The design option with the smallest environmental footprint combined a heat exchanger made out of polypropylene-graphite (PP-GR) with polyethylene pipework, exhibiting 80-99% less environmental impact compared with components made out of (35% recycled) copper. Contrasting the environmental impacts of two heat recovery set-ups with energy savings shows that a PP-GR based system pays back all burdens of the seven assessed environmental impact categories, within two years, while payback times for the copper-based system vary depending on the replaced energy source, and can exceed the 10 year operational lifetime of the system. When looking at typical flow-rates in UK food outlets, net environmental savings can be realised across all analysed impact categories above a threshold water consumption of 555 L/day, using current technology. Extrapolation to the UK food service sector indicates annual greenhouse gas emission mitigation potential of about 500 Gg CO2 equivalent.

Key performance indicators to explain energy & economic efficiency across water utilities, and identifying suitable proxies.

Water companies consume up to 8% of global energy demand, at billions of dollars' cost. Benchmarking of performance between utilities can facilitate improvements in efficiency; however, inconsistencies in benchmarking practices may obscure pathways to improvement. The aspiration was to conduct an unbiased efficiency comparison within a sample of 17 water only companies and water and sewerage companies in England and Wales, accounting for exogenous factors, whilst evaluating the accuracy of common proxies. Proxies were tested, and bias-corrected energy and economic efficiency scores with explanatory factors were analysed using a double-bootstrap data envelopment method. Bias correction altered the rankings of two companies for energy efficiency only. Results imply that on average, companies could reduce energy inputs by 91.7%, and economic inputs by 92.3%, which was symptomatic of the companies specialising in drinking water supply considerably out-performing combined water and sewerage companies. As exogenous influences were likely to be a factor in the disparity between the companies, five indicators were evaluated. The results varied but of note were average pumping head height, which displayed a significant negative effect for energy efficiency, and proportion of water passing through the largest four treatment works, that exhibited a significant negative effect on economic efficiency. Within proxy performance, population served for drinking water was an adequate replacement for volume of water produced, with results matching the core variable apart from two companies changing rank in the economic analysis. Conversely, length of water mains performed poorly when replacing capital expenditure, implying companies were on average 12.6% more efficient, resulting in ten companies changing their rank and causing explanatory variables to contradict direction of influence and significance. The findings contribute new insights for benchmarking, including how different types of water companies perform under bias-correcting methods, the degree to which factors affect efficiency and how appropriate some proxies are.

Economic and environmental efficiency of UK and Ireland water companies: Influence of exogenous factors and rurality.

For water companies, benchmarking their performance relative to other companies can be an effective way to identify the scope for efficiency gains to be made through infrastructure investment and operational improvements. However, a key limitation to benchmarking is the confounding effect of exogenous factors, which may not be factored in to benchmarking methodologies. The purpose of this study was to provide an unbiased comparison of efficiency across a sample of water and sewage companies, accounting for important exogenous factors. Bias-corrected economic and environmental efficiency estimates with explanatory factors were evaluated for a sample of 13 water and sewage companies in the UK and Ireland, using a double-bootstrap data envelopment analysis (DEA) approach. Bias correction for economic and environmental efficiency changed the rankings of nine and eight companies, respectively. On average, companies could reduce economic inputs by 19% and carbon outputs by 16% if they performed at the efficiency frontier. Variables explaining efficiency were: source of water, leakage rate, per capita consumption and population density. Population density showed statistical significance with both economic (p-value 0.002) and environmental (p-value 0.001) efficiency. Consequently, a rurality factor was defined for each company's operational area, which was then regressed against normalised water company performance data. More rural water companies spend more per property (R2 of 0.633), in part reflecting a larger number of smaller sewage treatment works serving rural populations (R2 of 0.823). These findings provide new insight into methods for benchmarking, and factors affecting, water company efficiency, pertinent for both regulators and water companies.

Current and Future Environmental Balance of Small-Scale Run-of-River Hydropower.

Globally, the hydropower (HP) sector has significant potential to increase its capacity by 2050. This study quantifies the energy and resource demands of small-scale HP projects and presents methods to reduce associated environmental impacts based on potential growth in the sector. The environmental burdens of three (50-650 kW) run-of-river HP projects were calculated using life cycle assessment (LCA). The global warming potential (GWP) for the projects to generate electricity ranged from 5.5-8.9 g CO2 eq/kWh, compared with 403 g CO2 eq/kWh for UK marginal grid electricity. A sensitivity analysis accounted for alternative manufacturing processes, transportation, ecodesign considerations, and extended project lifespan. These findings were extrapolated for technically viable HP sites in Europe, with the potential to generate 7.35 TWh and offset over 2.96 Mt of CO2 from grid electricity per annum. Incorporation of ecodesign could provide resource savings for these HP projects: avoiding 800 000 tonnes of concrete, 10 000 tonnes of steel, and 65 million vehicle miles. Small additional material and energy contributions can double a HP system lifespan, providing 39-47% reductions for all environmental impact categories. In a world of finite resources, this paper highlights the importance of HP as a resource-efficient, renewable energy system.

Changes in the physicochemical properties and enzymatic activity of waste during bioreduction of pig carcasses.

Bioreduction is a novel method for the on-farm storage of fallen stock in a vessel containing water that is heated and aerated, prior to disposal. The combination of a mesophilic temperature and high bacterial population leads to rapid degradation of carcasses due to microbial and enzymatic breakdown of protein material; and ultimately the reduction in volume of waste to be disposed. The system could, however, be improved if more was known about the changes that occur during a bioreduction cycle. Pig carcasses were placed within two commercial-scale bioreduction vessels (BVs) (6.5 m3 capacity) and the changes in physicochemical parameters, enzymatic activity, gas emissions and microbial communities were analysed over 56 days. Analyses showed that each vessel displayed different physicochemical parameters. The microbial communities within both vessels were also distinct, though they converged between days 28 and 42 before again diverging. Of the enzymes assayed, acetylesterases showed the highest activity during initial stages, with a subsequent increase in lipase towards the end. All other enzymes showed little activity in comparison. Despite active aeration of the vessels, conditions were redox-constrained, leading to the emission of gases associated with anaerobic conditions, namely NH3 and H2S. It was concluded that no single parameter governed the biochemical processes and that each BV will have its own unique microbial population and hence rate of decomposition. Further work is needed to increase the rate of bioreduction through bioaugmentation or developing enzyme additives.

Subclinical infection and asymptomatic carriage of gastrointestinal zoonoses: occupational exposure, environmental pathways, and the anonymous spread of disease.

Asymptomatic carriage of gastrointestinal zoonoses is more common in people whose profession involves them working directly with domesticated animals. Subclinical infections (defined as an infection in which symptoms are either asymptomatic or sufficiently mild to escape diagnosis) are important within a community as unknowing (asymptomatic) carriers of pathogens do not change their behaviour to prevent the spread of disease; therefore the public health significance of asymptomatic human excretion of zoonoses should not be underestimated. However, optimal strategies for managing diseases where asymptomatic carriage instigates further infection remain unresolved, and the impact on disease management is unclear. In this review we consider the environmental pathways associated with prolonged antigenic exposure and critically assess the significance of asymptomatic carriage in disease outbreaks. Although screening high-risk groups for occupationally acquired diseases would be logistically problematical, there may be an economic case for identifying and treating asymptomatic carriage if the costs of screening and treatment are less than the costs of identifying and treating those individuals infected by asymptomatic hosts.

Bioreduction of sheep carcasses effectively contains and reduces pathogen levels under operational and simulated breakdown conditions.

Options for the storage and disposal of animal carcasses are extremely limited in the EU after the introduction of the EU Animal By-products Regulations (ABPR; EC/1774/2002), leading to animosity within the livestock sector and the call for alternative methods to be validated. Novel storage technologies such as bioreduction may be approved under the ABPR provided that they can be shown to prevent pathogen proliferation. We studied the survival of Enterococcus faecalis, Salmonella spp., E. coli O157 and porcine parvovirus in bioreduction vessels containing sheep carcasses for approximately 4 months. The vessels were operated under two different scenarios: (A) where the water within was aerated and heated to 40 °C, and (B) with no aeration or heating, to simulate vessel failure. Microbial analysis verified that pathogens were contained within the bioreduction vessel and indeed reduced in numbers with time under both scenarios. This study shows that bioreduction can provide an effective and safe on-farm storage system for livestock carcasses prior to ultimate disposal. The findings support a review of the current regulatory framework so that bioreduction is considered for approval for industry use within the EU.

The role of the natural environment in the emergence of antibiotic resistance in gram-negative bacteria.

During the past 10 years, multidrug-resistant Gram-negative Enterobacteriaceae have become a substantial challenge to infection control. It has been suggested by clinicians that the effectiveness of antibiotics is in such rapid decline that, depending on the pathogen concerned, their future utility can be measured in decades or even years. Unless the rise in antibiotic resistance can be reversed, we can expect to see a substantial rise in incurable infection and fatality in both developed and developing regions. Antibiotic resistance develops through complex interactions, with resistance arising by de-novo mutation under clinical antibiotic selection or frequently by acquisition of mobile genes that have evolved over time in bacteria in the environment. The reservoir of resistance genes in the environment is due to a mix of naturally occurring resistance and those present in animal and human waste and the selective effects of pollutants, which can co-select for mobile genetic elements carrying multiple resistant genes. Less attention has been given to how anthropogenic activity might be causing evolution of antibiotic resistance in the environment. Although the economics of the pharmaceutical industry continue to restrict investment in novel biomedical responses, action must be taken to avoid the conjunction of factors that promote evolution and spread of antibiotic resistance.

Survival and metabolic activity of lux-marked Escherichia coli O157:H7 in different types of milk.

Escherichia coli O157:H7 is a potentially lethal pathogen which has been responsible for several outbreaks of milk-borne illness in recent years. The objective of this study was to evaluate the survival and metabolic activity (indexed by bioluminescence) of a chromosomally lux-marked strain of Esch. coli O157:H7 in raw, pasteurized and microfiltered pasteurized milk at 4 and 20°C for up to 14 d. Results showed that the population of Esch. coli O157:H7 and its metabolic activity decreased in all samples during storage at 4°C, with no significant differences in numbers observed between the different milk types; but metabolic activity was significantly higher (P<0·05) in the microfiltered pasteurized milk than that in raw milk. At 20°C, Esch. coli O157:H7 counts and cell activity peaked at day 2, and then declined progressively. At 20°C, survival and metabolic activity were significantly lower in raw milk compared with pasteurized milk. We conclude that storage temperature is more important in regulating the survival of Esch. coli O157 in contaminated milk than its origin/pre-treatment conditions.

Lettuce cultivar mediates both phyllosphere and rhizosphere activity of Escherichia coli O157:H7.

Plant roots and leaves can be colonized by human pathogenic bacteria, and accordingly some of the largest outbreaks of foodborne illness have been associated with salad leaves contaminated by E. coli O157. Integrated disease management strategies often exploit cultivar resistance to provide a level of protection from economically important plant pathogens; however, there is limited evidence of whether the genotype of the plant can also influence the extent of E. coli O157 colonization. To determine cultivar-specific effects on colonization by E. coli O157, we used 12 different cultivars of lettuce inoculated with a chromosomally lux-marked strain of E. coli O157:H7. Lettuce seedlings grown gnotobiotically in vitro did exhibit a differential cultivar-specific response to E. coli O157 colonization, although importantly there was no relationship between metabolic activity (measured as bioluminescence) and cell numbers. Metabolic activity was highest and lowest on the cultivars Vaila-winter gem and Dazzle respectively, and much higher in endophytic and tightly bound cells than in epiphytic and loosely bound cells. The cultivar effect was also evident in the rhizosphere of plants grown in compost, which suggests that cultivar-specific root exudate influences E. coli O157 activity. However, the influence of cultivar in the rhizosphere was the opposite to that in the phyllosphere, and the higher number and activity of E. coli O157 cells in the rhizosphere may be a consequence of them not being able to gain entry to the plant as effectively. If metabolic activity in the phyllosphere corresponds to a more prepared state of infectivity during human consumption, leaf internalization of E. coli O157 may pose more of a public health risk than leaf surface contamination alone.

Fate of pathogens in a simulated bioreduction system for livestock carcasses.

The EU Animal By-Products Regulations generated the need for novel methods of storage and disposal of dead livestock. Bioreduction prior to rendering or incineration has been proposed as a practical and potentially cost-effective method; however, its biosecurity characteristics need to be elucidated. To address this, Salmonella enterica (serovars Senftenberg and Poona), Enterococcus faecalis, Campylobacter jejuni, Campylobacter coli and a lux-marked strain of Escherichia coli O157 were inoculated into laboratory-scale bioreduction vessels containing sheep carcass constituents. Numbers of all pathogens and the metabolic activity of E. coli O157 decreased significantly within the liquor waste over time, and only E. faecalis remained detectable after 3months. Only very low numbers of Salmonella spp. and E. faecalis were detected in bioaerosols, and only at initial stages of the trial. These results further indicate that bioreduction represents a suitable method of storing and reducing the volume of livestock carcasses prior to ultimate disposal.

The environmental and biosecurity characteristics of livestock carcass disposal methods: A review.

Livestock mortalities represent a major waste stream within agriculture. Many different methods are used throughout the world to dispose of these mortalities; however within the European Union (EU) disposal options are limited by stringent legislation. The legal disposal options currently available to EU farmers (primarily rendering and incineration) are frequently negatively perceived on both practical and economic grounds. In this review, we assess the potential environment impacts and biosecurity risks associated with each of the main options used for disposal of livestock mortalities in the world and critically evaluate the justification for current EU regulations. Overall, we conclude that while current legislation intends to minimise the potential for on-farm pollution and the spread of infectious diseases (e.g. transmissible spongiform encephalopathies, bacterial pathogens), alternative technologies (e.g. bioreduction, anaerobic digestion) may provide a more cost-effective, practical and biosecure mechanism for carcass disposal as well as having a lower environmental footprint. Further social, environmental and economic research is therefore warranted to assess the holistic benefits of alternative approaches for carcass disposal in Europe, with an aim to provide policy-makers with robust knowledge to make informed decisions on future legislation.

Use of a Randomized Response Technique to obtain sensitive information on animal disease prevalence.

In order for policy-makers to formulate effective disease control measures they require accurate estimates of the extent and prevalence of the disease. On occasion, obtaining these data can be difficult, as farmers may perceive relevant information as being sensitive to divulge. Consequently, underestimation of disease prevalence may occur due to farmer reticence about admitting to behaviours and outcomes that are considered professionally transgressive. To overcome such weaknesses, this study used a novel social science survey method known as the Randomized Response Technique in order to obtain population level estimations of transgressionary behavior and disease prevalence for the case of sheep scab in Wales. Since deregulation in 1992, sheep scab has become widespread throughout the UK. Previous estimates of sheep scab in the UK have employed survey techniques which do not adequately protect the anonymity of the farmers' answers other than via verbal or institution backed promise. In this study, farmers at several agricultural shows in Wales were asked to complete questionnaires according to the Randomized Response Technique during the summer of 2009. The survey results suggest that 30% of farmers did not routinely treat their sheep for scab. A further 36.5% of the surveyed farmers admitted to having sheep scab in their flocks in the past 5 years. These estimates are both higher than obtained by previous surveys which used more traditional questionnaire structures. Survey responses revealed a strong willingness to reintroduce compulsory treatment measures to control or eradicate the disease. This is the first study to demonstrate the value of the Randomized Response Technique in the context of animal health.

In-vessel bioreduction provides an effective storage and pre-treatment method for livestock carcasses prior to final disposal.

The EU Animal By-Products Regulations forbid the burial of livestock carcasses on land. Farmers would benefit from the availability of biosecure and economically viable alternatives for storing and disposing of dead animals. We assessed the efficacy of bioreduction vessels as a mechanism of storing and reducing the volume of fallen livestock prior to ultimate disposal. Two experimental scenarios were tested: (1) a single input of 300 kg of dead sheep with no further inputs for 3 months, and (2) a continuous 'on-farm' addition of dead sheep over 12 months (ca. 2-3t animals vessel(-1)). The trials involved half-filling the vessels with water, addition of the dead sheep with subsequent heating (40 degrees C) and aeration of the liquor. Each trial was repeated three times. Our results showed a complete biodigestion and liquefaction of the animals alongside a significant bioreduction in the volume of the liquid. No pathogens could be detected in either the waste or the gaseous emissions. Calculations showed that bioreduction may offer significant long-term savings for farmers in terms of animal disposal costs. Our findings suggest that bioreduction may offer a practical, biosecure, and cost-effective method of storing fallen livestock prior to disposal via rendering or incineration.