RESUMEN
Two new lanostanoid triterpenes, 23S-hydroxy-3,7,11,15-tetraoxo-lanost-8,24E-diene-26-oic acid (1), and 12beta-acetoxy-3beta-hydroxy-7,11,15,23-tetraoxo-lanost-8,20E-diene-26-oic acid (16), together with 17 known compounds, were isolated from the fruit bodies of Ganoderma lucidum. Their structures were established by spectroscopic methods, especially 2D-NMR and MS analyses and by comparison with literature data. The cytotoxic assay of the above compounds against p388, Hela, BEL-7402, and SGC-7901 human cancer cell lines showed their cytotoxicity with the IC50 values in the range of 8-25 microm.
Asunto(s)
Antineoplásicos Fitogénicos/química , Reishi/química , Triterpenos/química , Antineoplásicos Fitogénicos/farmacología , Línea Celular Tumoral , HumanosRESUMEN
Three new lignan glycosides (1-3) were isolated from the stems of Akebia trifoliata. Their structures were elucidated as (7R,8R,7'R,8'R)3,3',5,5'tetramethoxy-4,4'dihydroxy-7,9':7',9-diepoxylignan-4-O-beta-D-glucopyranoside (1), (7S,8S,8'R)-4,4',9-trihydroxy-3,3',5,5'-tetramethoxy-7,9'-epoxylignan-7'-one 9-O-beta-D-glucopyranoside (2), (7R,8R,8'S)-4,4',9-trihydroxy3,3',5,5'-tetramethoxy-7,9'-epoxylignan-7'-one 9-O-beta-D-glucopyranoside (3) by spectral analyses, primarily NMR, MS and CD. The NMR assignments for the compounds were carried out using 1H, 13C, DEPT, COSY, HSQC, HMBC and ROESY NMR experiments.
Asunto(s)
Medicamentos Herbarios Chinos/química , Glucósidos/química , Glucósidos/síntesis química , Glicósidos/química , Lignanos/química , Resonancia Magnética Nuclear Biomolecular , Ranunculaceae/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Glucósidos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Lignanos/síntesis química , Lignanos/aislamiento & purificación , Modelos Moleculares , Conformación Molecular , Tallos de la Planta/químicaRESUMEN
Three new lanostanoid triterpenes named 3beta,7beta-di- hydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (1), 3beta,7beta-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid methyl ester (2), and 12beta-acetoxy-3beta,7beta-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (3) were isolated from the fruit bodies of Ganoderma lucidum. They all show a Delta(16, 17) double bond unprecedented in such types of lanostanoid triterpenes possessing the side chain at C-17. The complete NMR assignments for these compounds were carried out using (1)H, (13)C, DEPT, COSY, HSQC, HMBC, and ROESY NMR experiments.
Asunto(s)
Lanosterol/análogos & derivados , Reishi/química , Triterpenos/química , Cuerpos Fructíferos de los Hongos/química , Lanosterol/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría UltravioletaRESUMEN
A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mmx4.6 mm, 5 microm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2>0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.
