Shared insights for heat health risk adaptation in metropolitan areas of developing countries.

Global warming has led to a surge in heat health risks (HHRs), the impacts of which are particularly pronounced in metropolitan areas of developing countries. In the current study, six metropolitan areas - Beijing, China; Cairo, Egypt; Jakarta, Indonesia; Mumbai, India; Rio de Janeiro, Brazil; and Tehran, Iran - were selected as the study area to further differentiate the built-up landscapes by utilizing the concept of local climate zones. Moreover, we assessed the similarities and differences in HHR associated with the landscape. Results revealed a 30.67% higher HHR in compact built-up landscapes than in the open built-up type. Urban green spaces played an effective but differentiated role in mitigating HHR. That is, low vegetation in urbanized areas and trees in suburban areas significantly mitigated HHR. Collectively, our findings emphasize the role of effective planning and management in addressing HHR and provide empirical support for implementing HHR mitigation and adaptation strategies.

Divergence of the freshwater sleeper, Neodontobutishainanensis (Chen, 1985) (Teleostei, Odontobutidae), in the Pearl River basin and on Hainan Island of southern China.

Study of divergence of freshwater fish populations between island and adjacent mainland areas can shed light on the phylogeographical relationships of these regions. Neodontobutishainanensis is a freshwater fish species restricted to Hainan Island and in Guangdong and Guangxi provinces in the southern mainland China. We examine the phylogenetic relationship and population structure of N.hainanensis based on 3,176 nuclear loci using a gene-capture method. STRUCTURE analysis and principal coordinate analyses (PCA) indicate that populations from Guangdong, Guangxi and Hainan are each distinct, except that some individuals of the Guangdong population share minor genetic components with individuals of the Guangxi population. In the concatenated gene tree, the Hainan population is grouped with the Guangdong population, but the coalescent tree groups the Hainan population as the sister to the Guangxi population. Finally, coalescent simulations confirmed the divergence pattern supported by the coalescent tree and revealed a one-way introgression from the Guangxi population to the Guangdong population, which can explain the discordant results supported by the concatenated and coalescent phylogenetic analyses. Due to recent decline of N.hainanensis populations and the genetic patterns in this species, as revealed in this study, the populations in the three areas should be treated as separate conservation units.

Analysis of Breast Cancer Screening Results and Influencing Factors of Breast Cancer in Guangdong Province from 2017 to 2021.

BACKGROUNDS: Breast cancer screening plays an important role in the early detection, diagnosis and treatment of breast cancer. The aim of this study was to evaluate the screening results and explore the influencing factors of breast cancer detection rate in Guangdong. METHODS: This cross-sectional study was conducted among 2,024,960 women aged 35-64 in Guangdong Province during 2017-2021. The data about breast cancer screening information were collected from the Guangdong maternal and child health information system. Descriptive statistical analysis was used to explain demographic characteristics and results of breast cancer screening. The generalized linear regression model was applied to analyze the related influencing factors of breast cancer detection rate. RESULTS: The estimated detection rate of breast cancer in Guangdong Province is 70.32/105, with an early diagnosis rate of 82.06%. After adjusting covariates, those women with older age (45-55 [OR (95% CI) 2.174 (1.872, 2.526)], 55-65 [OR (95% CI) 2.162 (1.760, 2.657)]), education for high school ([OR (95% CI) 1.491 (1.254, 1.773)]) and older age at first birth ([OR (95% CI) 1.632 (1.445, 1.844)]) were more likely to have higher detection rate of breast cancer. No history of surgery or biopsy ([OR (95% CI) 0.527 (0.387, 0.718)]), no history of breast cancer check ([OR (95% CI) 0.873 (0.774, 0.985)]) and no family history of breast cancer ([OR (95% CI) 0.255 (0.151, 0.432)]) women were more likely to screen negative for breast cancer (P < 0.05). CONCLUSION: The detection rate of breast cancer in screening showed an increasing trend year by year in Guangdong Province. Older age, education for high school and older age at first birth were risk factors for breast cancer detection rate, while no surgery or biopsy history, no family history of breast cancer and no history of breast cancer check were protective factors.

Effects of Species Invasion and Inundation on the Collembola Community in Coastal Mudflat Wetland from the Perspective of Functional Traits.

The group of soil arthropods known as Collembola is characterized by its abundance and sensitivity to environmental changes. They are ideal an species for soil indicators. In order to clarify the effects of species invasion and inundation on the Collembola community in coastal mudflat wetlands, the correlation between the collembolan functional traits and environmental factors was studied in Shanghai Jiuduansha Wetland National Nature Reserve for the first time. Five sample plots, including three vegetations-Spartina alterniflora (an invasive species), Phragmites australis, and Zizania latifolia-were set up following the differences in vegetation types and between high and low tidal flats. Data on the diversity of the Collembolan species and their functional traits were collected and combined with the soil physicochemical properties and vegetation environment factors in different tidal flats. The key findings and conclusions of the study are as follows: a total of 18 species, four families, and three orders make up the obtained Collembola, two species of Proisotoma are dominant species that account for 49.59% and 24.91% of the total, respectively. The maintenance of the species diversity of Collembola is disturbed by the higher conversion efficiency of Spartina alterniflora rather than Phragmites australis with lower organic carbon (C) content and higher total nitrogen (N) content. The primary environmental variables influencing species distribution were the C/N ratio, total N, and bulk soil density. The bulk density of the soil impacts the movement and dispersal of the functional traits. The depth of the soil layer is related to the functional traits of the sensory ability. The analysis of the functional traits and environment is fairly helpful in exploring how species respond to their environment and offers a better explanation for the habitat selection of Collembola.

Variations of Urban Thermal Risk with Local Climate Zones.

Due to the differences in land cover and natural surroundings within cities, residents in various regions face different thermal risks. Therefore, this study combined multi-source data to analyze the relationship between urban heat risk and local climate zones (LCZ). We found that in downtown Shenyang, the building-type LCZ was mainly found in urban centers, while the natural- type LCZ was mainly found in suburbs. Heat risk was highest in urban centers, gradually decreasing along the suburban direction. The thermal risk indices of the building-type LCZs were significantly higher than those of the natural types. Among the building types of LCZs, LCZ 8 (open middle high-rise) had the highest average thermal risk index (0.48), followed by LCZ 3 (0.46). Among the natural types of LCZs, LCZ E (bare rock and paved) and LCZ F (bare soil and sand) had the highest thermal risk indices, reaching 0.31 and 0.29, respectively. This study evaluated the thermal risk of the Shenyang central urban area from the perspective of LCZs and combined it with high-resolution remote sensing data to provide a reference for thermal risk mitigation in future urban planning.

Regional thermal environment changes: Integration of satellite data and land use/land cover.

Land surface temperature (LST) is subject to location and environmental influences, which makes quantification difficult in terms of timeliness. Based on 10-d geostationary satellite LST TCI products, we quantitatively evaluated the thermal environment differentiation of various ground objects in North, South, and Northwest China from 2017 to 2021. We found that the thermal condition index (TCI) in Northwest China decreased, whereas it increased in North and South China. In contrast, Moran's I index increased in Northwest and South China, with strong spatial agglomeration. The TCI for artificial surfaces decreased from North (0.633) to Northwest (0.554) and South China (0.384). The bare land TCI was always the lowest among the land use/land cover (LULC) types in each region. Our results reflect the LULC thermal environment of China against the background of new urbanization and provide theoretical support for scientific planning to improve the ecological environment.

Efficacy and safety of acupuncture for depression: A systematic review and meta-analysis.

Acupuncture is widely accepted as a therapeutic option for managing depression. However, evidence from clinical trials remains controversial. This review aims to synthesize the best available evidence on the efficacy and safety of acupuncture in managing depression. The review was performed according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. Five databases and the relevant trial registries were searched from the inception to October 2021. Randomized clinical trials of acupuncture for managing depression, published in English, were selected for inclusion. The quality of included studies was assessed using the Cochrane risk of bias tool. Netmeta and dmetar of R packages were used to conduct a network meta-analysis. Twenty-two trials with 2391 participants were eligible and included in the analysis. This review found evidence that electroacupuncture (EA) plus antidepressant achieved superior outcomes compared with the waitlist (standardized mean difference = -8.86, 95% confidence interval: -14.78 to -2.93). The treatment ranking of different interventions in improving depression symptoms indicated that EA plus antidepressant with a probability of 0.8294, followed by manual acupuncture (MA) plus antidepressant (0.6470) and MA (0.5232). Acupuncture, either in isolation or as an adjunct to pharmacological treatment, has clinical benefits and can be considered a safe option for managing depression.

Role of vascular endothelial growth factor in radiotherapy resistance to esophageal squamous cell carcinoma.

Vascular endothelial growth factor (VEGF) is related to the radiation resistance of tumors, resulting in the failure of tumor radiotherapy. The purpose of this study was to discuss the role of VEGF in radiotherapy resistance of esophageal squamous cell carcinoma (ESCC). We used the VEGF kit by ELISA to detect the serum VEGF level of ESCC patients who only received radiotherapy. The expression of VEGF in ESCC cells after siRNA treatment was verified by Western blot. The sensitivity of ESCC cells to radiation after knocking down VEGF was analyzed by Clonogenic assay and Cell counting kit (CCK-8). The results showed that the level of serum VEGF in patients with ESCC before and after radiotherapy was related to the clinical response, and it was confirmed that knocking down the expression of VEGF in ESCC cells improved the sensitivity to radiation.

Modeling the decision of ridesourcing drivers to park and wait at trip ends: a comparison between Perth, Australia and Kolkata, India.

It is often difficult for the ridesourcing drivers to get a trip immediately after dropping off a passenger. The main objective of the drivers is to increase their income by serving more trips. The most prominent options available to the drivers after reaching passengers' destinations are: (a) park and wait in and around their drop-off location, (b) cruise in and around their drop-off location and (c) drive to another location to receive trip requests quickly. Previous studies were conducted to understand the driver behaviour in a taxi and other similar services. However, the perception of ridesourcing drivers on parking and waiting after dropping off passengers is yet to be explored. The drivers' decision on waiting can affect users' waiting time, the number of matched trips by the TNCs, and parking spaces in the city. Moreover, drivers' waiting time tolerance can also impact other drivers' total number of trips, total earnings, total distance travelled in the city, and fleet size. The aim of this study is to understand the influence of drivers' characteristics on drivers' decision to park and wait after dropping off a passenger. This study estimates and compares the waiting time tolerance of the ridesourcing drivers using a zero-inflated cox spline model between Perth and Kolkata. It is observed that drivers in Kolkata have higher waiting time tolerance than Perth drivers. Moreover, the drivers in both the cities are more likely to wait at high-demand areas urging the urban authorities to determine spatio-temporal parking demand to design the parking infrastructure for such areas.

Warming and cooling effects of local climate zones on urban thermal environment.

Understanding the thermal characteristics and contribution ranking of local climate zones (LCZs) is essential since they can help in maintaining environmental harmony. However, previous studies only considered independent effects and could not analyze the combined effects of LCZ on land surface temperature (LST). In this study, we propose a new method to establish an interaction model between LCZs. Five first-level grids with different scales from 270 to 990 m were established to calculate the area proportion of LCZ. The area proportion of LCZ was then applied in the stepwise regression model to quantitatively analyze its magnitude and direction of impact on the LST. The results suggest that the LCZ types of the study area with the highest and lowest average LST were LCZ2 (compact middle-rise building, 39.82°C) and LCZG (water body, 34.24°C), respectively. However, on most scales, the warming effect of LCZ2 was lower than that of LCZE (bare rock or paver), and the cooling effect of LCZG was lower than that of LCZD (low plants). The optimum results were obtained at a scale of 810 m. At this scale, the warming effect was in the order: LCZE (0.314) > LCZ2 (0.236) > LCZ3 (compact low-rise building, 0.135) > LCZ5 (open middle-rise, 0.084) > LCZ6 (open low-rise, 0.056); the cooling effect was in the order: LCZD (-0.272) > LCZA (dense trees, -0.104) > LCZG (-0.103). These findings can help to elucidate the unique warming and cooling effects of LCZ on the interaction condition and the construction of an urban human settlement.

Management Algorithm for Prevention of Mother-to-child Transmission of Hepatitis B Virus (2022).

The World Health Organization (WHO) has set the goal of eliminating hepatitis as a threat to public health by 2030. Blocking mother-to-child transmission (MTCT) of hepatitis B virus (HBV) is not only the key to eliminating viral hepatitis, but also a hot issue in the field of hepatitis B prevention and treatment. To standardize the clinical management of preventing MTCT of HBV and achieve zero HBV infection among infants, the Chinese Foundation for Hepatitis Prevention and Control organized experts to compile a management algorithm for prevention of MTCT of HBV based on the latest research progress and guidelines, including 10 steps of pregnancy management and postpartum follow-up, among which screening, antiviral treatment, and infant immunization are its core components.

Associations between maternal mid-pregnancy apolipoprotein A-1, apolipoprotein B, apolipoprotein B/apolipoprotein A-1 ratio and preterm birth.

BACKGROUND AND AIMS: Elevated lipid levels during pregnancy have been shown to be related to the risk of preterm birth. Despite the importance of apolipoprotein (Apo) in lipid metabolism and transportation, evidence regarding apolipoprotein levels during pregnancy and preterm birth is still limited. Therefore, we aim to investigate the associations between maternal ApoA-1, ApoB, ApoB/ApoA-1 ratio and preterm birth. MATERIALS AND METH: Data were extracted from the information system of Guangdong Women and Children Hospital. Lipoprotein levels were tested using Beckman Coulter AU5800 in mid-pregnancy at a median gestational age of 18 w. Maternal serum ApoB, ApoA-1 and ApoB/ApoA-1 ratio were categorized into tertiles. Logistic regression models were performed to evaluate the odds ratios and 95% confidence intervals for preterm birth. RESULTS: A total of 5,986 maternal-newborn pairs were included in this study. The rate of preterm birth was 5.7% (n = 344). The multivariate-adjusted ORs (95% CI) of preterm birth were 1.51 (1.06, 2.10) for individuals with high ApoB (>90th), 0.63 (0.38, 0.99) for those with low ApoB (<10th), and 1.64 (1.18, 2.24) for those with high ApoB/ApoA-1 (>90th). Subgroup analyses showed that the association of ApoB and preterm birth was only significant among women with pre-pregnancy BMI 18.5-24 kg/m2 (OR = 1.36, 95% CI: 1.12-1.65), age at delivery ≥ 35 years (OR = 1.43, 95% CI: 1.12-1.83). CONCLUSION: Elevated maternal ApoB level and ApoB/ApoA-1 ratio during mid-pregnancy were related to increased risk of preterm birth. Monitoring maternal serum apolipoprotein levels may help to identify the high-risk population of preterm birth.

Exploring seasonal diurnal surface temperature variation in cities based on ECOSTRESS data: A local climate zone perspective.

High urban temperatures affect city livability and may be harmful for inhabitants. Analyzing spatial and temporal differences in surface temperature and the thermal impact of urban morphological heterogeneity can promote strategies to improve the insulation of the urban thermal environment. Therefore, we analyzed the diurnal variation of land surface temperature (LST) and seasonal differences in the Fifth Ring Road area of Beijing from the perspective of the Local Climate Zone (LCZ) using latest ECOSTRESS data. We used ECOSTRESS LST data with a resolution of 70 m to accurately interpret the effects of urban morphology on the local climate. The study area was dominated by the LCZ9 type (sparse low-rise buildings) and natural LCZ types, such as LCZA/B (woodland), LCZD (grassland), and LCZG (water body), mainly including park landscapes. There were significant differences in LST observed in different seasons as well as day and night. During daytime, LST was ranked as follows: summer > spring > autumn > winter. During night-time, it was ranked as follows: summer > autumn > spring > winter. All data indicated that the highest and lowest LST was observed in summer and winter, respectively. LST was consistent with LCZ in terms of spatial distribution. Overall, the LST of each LCZ during daytime was higher than that of night-time during different seasons (except winter), and the average LST of each LCZ during the diurnal period in summer was higher than that of other seasons. The LST of each LCZ during daytime in winter was lower than that of the corresponding night-time, which indicates that it is colder in the daytime during winter. The results presented herein can facilitate improved analysis of spatial and temporal differences in surface temperature in urban areas, leading to the development of strategies aimed at improving livability and public health in cities.

Contribution of local climate zones to the thermal environment and energy demand.

Urban heat islands (UHIs) and their energy consumption are topics of widespread concern. This study used remote sensing images and building and meteorological data as parameters, with reference to Oke's local climate zone (LCZ), to divide urban areas according to the height and density of buildings and land cover types. While analyzing the heat island intensity, the neural network training method was used to obtain temperature data with good temporal as well as spatial resolution. Combining degree-days with the division of LCZs, a more accurate distribution of energy demand can be obtained by different regions. Here, the spatial distribution of buildings in Shenyang, China, and the law of land surface temperature (LST) and energy consumption of different LCZ types, which are related to building height and density, were obtained. The LST and energy consumption were found to be correlated. The highest heat island intensity, i.e., UHILCZ 4, was 8.17°C. The correlation coefficients of LST with building height and density were -0.16 and 0.24, respectively. The correlation between urban cooling energy demand and building height was -0.17, and the correlation between urban cooling energy demand and building density was 0.17. The results indicate that low- and medium-rise buildings consume more cooling energy.

Population Structure, Genetic Diversity, and Conservation Strategies of a Commercially Important Sleeper Fish, Odontobutis potamophilus (Gobiiformes: Odontobutidae) Based on Gene-Capture Data.

Odontobutis potamophilus is a popular food fish in China, distributed mainly in the middle and lower reaches of the Yangtze River, where it is a famous delicacy and a newly focused species for aquaculture. The wild populations of O. potamophilus are facing the problem of overfishing and habitat degradation. Therefore, it is very necessary to investigate and protect the wild populations of O. potamophilus. In this study, 72 fish were sampled from 18 different sites over its distribution range. Nuclear sequence data of 4,267 loci were collected using a gene-capture method. Phylogenetic reconstruction revealed that there were three major clades: Oujiang clade (OJ), Qiantang and lower Yangtze clade (QY), and middle Yangtze clade (MY). The discriminant analysis of principal components (DAPC) and a STRUCTURE analysis confirmed that there are three major groups within O. potamophilus. A fastsimcoal2 analysis corroborated the population history and suggested that there was discernible gene flow among these three groups, especially between QY and MY. Estimated pairwise F ST suggested that Linhai (LH) and Shexian (SX) populations were the most divergent pair (F ST = 0.7077). Taking the nucleotide diversity, population divergence, and admixture status altogether into consideration, we recommend that the LH, Gaoyou (GY) and Chaohu (CH) populations could be protected as the preferred resource for breeding projects. According to the results of genetic analyses, all populations of O. potamophilus should be protected due to low genetic diversity.

Urban ventilation corridors and spatiotemporal divergence patterns of urban heat island intensity: a local climate zone perspective.

Urban ventilation corridors introduce fresh air into urban interiors and improve urban livability, while mitigating the urban heat island (UHI) effect. However, few studies have assessed the impact of urban ventilation corridors on UHI intensity (UHII) from the perspective of the local climates of different cities. Therefore, this study integrated multisource data to construct ventilation corridors from the perspective of local climate zone (LCZ) and analyzed its impact on UHII. The results showed the following: (1) the average UHII of constructed LCZs was higher than that of natural LCZs, among which the building type LCZ10 (heavy industry) had the highest intensity (5.77 °C); (2) in extracted ventilation corridors, the pixel number of natural LCZs was substantially larger than that of constructed LCZs, among which LCZE (bare soil/paved) was the largest; and (3) for natural LCZs, the average UHII of each LCZ was lower within the ventilated corridors than within the non-ventilated corridors (except for LCZG [water]), with the UHII of LCZB (scattered trees) exhibiting the greatest mitigation effect. Quantitative research on the composition and function of ventilation corridors can not only assess the ability of ventilation corridors to mitigate UHIs, but also provide a reference for urban ventilation corridor planning.

Feasibility and therapeutic efficacy of a two-week low-level laser acupuncture therapy for shoulder and neck pain in office workers: Protocol for a pilot, single-blind, double-armed, randomised controlled trial.

BACKGROUND: Shoulder and neck pain (SNP) is common in office workers and represents a serious public health problem given its detrimental impact on quality of life, physical functioning, personal finances, employers, and the health care system. Management with painkillers has adverse implications such as tolerance, addiction, and opioid abuse. Safe, sustainable, cost-effective, and evidence-based solutions are urgently needed. The non-invasive, painless, non-infectious, and safe modality of low-level laser acupuncture (LLLA) has shown promise for SNP management. OBJECTIVE: The overarching aim of this study is to provide evidence of the feasibility and therapeutic efficacy of LLLA for office workers with SNP. METHODS: This is a pilot, single-blind, double-armed, randomised controlled trial on the feasibility and therapeutic efficacy of a two-week LLLA therapy for office workers with SNP, aged 18 to 65 years. Each of the two study groups will contain 35 participants: the intervention group will receive LLLA from a licensed acupuncturist at the researchers' university clinic (10-20 min/session, 3 sessions/week) for two weeks; the control group will receive usual care without painkillers. Outcomes will be measured at baseline, throughout the two-week intervention, and at trial end. Surveys including open-ended questions will be completed. The primary outcome of this study is to evaluate the feasibility of a two-week LLLA therapy for office workers with SNP, as measured by recruitment and completion rates, patient safety, and treatment adherence and compliance. Participants' attitudes, motivation, and challenges to participation, intervention non-compliance, and experience of participating in the trial will be investigated via qualitative data. The secondary outcome is to evaluate the therapeutic efficacy of LLLA on SNP using the visual analogue scale (VAS) and the Short-Form McGill Pain Questionnaire (SF-MPQ); the work productivity and activity assessment (WPAI:SHP); 12-Item Short Form Survey (SF-12) for quality of life assessment; and the past 3-month out-of-pocket (OOP) cost for prescription and non-prescription SNP therapy, which is an indicative of the economic burden of SNP on patients and health care systems. This study was approved by Edith Cowan University's Human Research Ethics Committee (No. 2021-02225-WANG). RESULTS: Data collection will commence in December 2021 with anticipated completion by December 2022. CONCLUSIONS: Safe, sustainable, cost-effective, evidence-based interventions are needed to minimise the negative implications of SNP in office workers. LLLA is a promising modality in managing SNP. However, more consolidated evidence is required to provide insight regarding the effectiveness of LLLA. This study is expected to contribute to the challenging work of reducing the burden of SNP in office workers. TRIAL REGISTRATION: Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN12621000426886p; https://www.anzctr.org.au/ACTRN12621000426886p.aspx.

The E3 Ligase MIB1 Promotes Proteasomal Degradation of NRF2 and Sensitizes Lung Cancer Cells to Ferroptosis.

Dysregulation of Notch signaling has been implicated in cellular transformation and tumorigenesis in a variety of cancers while potential roles of MIB1, an E3 ubiquitin ligase required for efficient Notch activation, remains to be investigated. We analyzed MIB1 expression levels in tumor samples and performed gain-of-function and loss-of-function studies in cell lines to investigate potential roles of MIB1 in epithelial-to-mesenchymal transition (EMT), cell migration, and cell survival. We found that overexpression of MIB1 is detected in a subset of lung squamous carcinoma and adenocarcinoma samples and negative correlation is observed between MIB1 expression and overall patient survival. Ectopic expression of MIB1 in A549 cells induces EMT and stimulates cell migration via a Notch-dependent pathway. Meanwhile, MIB1 stimulates the degradation of nuclear factor erythroid 2-related factor 2 (NRF2) in a Notch-independent manner and disrupts the antioxidant capacity of cells, rendering them more sensitive to inducers of ferroptosis. On the other hand, MIB1 knockout induces accumulation of NRF2 and resistance to ferroptosis. Collectively, these results indicate that MIB1 may function as a positive regulator of ferroptosis through targeted degradation of the master antioxidant transcription factor NRF2. IMPLICATIONS: This study identifies a MIB1-induced proteasomal degradation pathway for NRF2 and reveals elevated ferroptosis sensitivity in MIB1-overexpressing cells which may provide novel insights into the treatment of MIB1-overexpressing cancers.

A high-resolution genome of an euryhaline and eurythermal rhinogoby (Rhinogobius similis Gill 1895).

Rhinogobius similis is distributed in East and Southeast Asia. It is an amphidromous species found mostly in freshwater and sometimes brackish waters. We have obtained a high-resolution assembly of the R. similis genome using nanopore sequencing, high-throughput chromosome conformation capture (Hi-C), and transcriptomic data. The assembled genome was 890.10 Mb in size and 40.15% in GC content. Including 1373 contigs with contig N50 is 1.54 Mb, and scaffold N50 is 41.51 Mb. All of the 1373 contigs were anchored on 22 pairs of chromosomes. The BUSCO evaluation score was 93.02% indicating high quality of genome assembly. The repeat sequences accounted for 34.92% of the whole genome, with retroelements (30.13%), DNA transposons (1.64%), simple repeats (2.34%), and so forth. A total of 31,089 protein-coding genes were predicted in the genome and functionally annotated using Maker, of those genes, 26,893 (86.50%) were found in InterProScan5. There were 1910 gene families expanded in R. similis, 1171 gene families contracted and 170 gene families rapidly evolving. We have compared one rapidly change gene family (PF05970) commonly found in four species (Boleophthalmus pectinirostris, Neogobius melanostomus, Periophthalmus magnuspinnatus, and R. similis), which was found probably related to the lifespan of those species. During 400-10 Ka, the period of the Guxiang Ice Age, the population of R. similis decreased drastically, and then increased gradually following the last interglacial period. A high-resolution genome of R. similis should be useful to study taxonomy, biogeography, comparative genomics, and adaptive evolution of the most speciose freshwater goby genus, Rhinogobius.

Assessment of changes in environmental factors in a tourism-oriented Island.

Tourism development has influenced industrial structure changes and has become a major driving force for China's new urbanization. However, the development will negatively impact natural resources and the ecological environment and will become an essential driving factor for land use change. Therefore, understanding the impact of tourism urbanization is crucial for sustainable local development. This study selected the Dachangshan Island in the Changhai County, Dalian, China, as the study area, because it is the only coastal island-type border county in China. During the study period, changes in local environmental factors were analyzed based on land use data, Landsat 5 and Landsat 8 data of 2009, 2014, and 2019. The results showed that: (1) the overall land surface temperature (LST) in the research region shows an increasing trend; the LST in 2014 and 2019 increased by 6.10 and 5.94 °C, respectively, compared with 2009. With respect to specific land types, impervious surfaces maintained a high land surface temperature (25.44, 32.38, and 31.86); however, surface temperatures for cropland, forest, grassland, and water bodies remained stable. (2) The land use land cover (LULC) change analysis from 2009-2019 indicates that impervious surfaces and cropland increased by 0.5653 km2 and 0.9941 km2, while the areas of forest, grassland, and water bodies decreased. The results also showed that forests (-1.3703 km2) are most affected by urbanization. (3) The results of the landscape index calculation showed that the variation at the patch scale is different for different LULC types. The patch density of impervious surfaces decreased, but the aggregation index increased over time, while the patch density of the forest increased continuously. At the landscape scale, overall patch type and distribution remained stable. The purpose of this study is to explore the environmental changes of islands and provide a reference for the sustainable development of islands.