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Secondary iron-sulfate minerals such as jarosite, which are easily formed in acid mine drainage, play an important role in controlling metal mobility. In this work, the typical iron-oxidizing bacterium Acidithiobacillus ferrooxidans ATCC 23270 was selected to synthesize jarosite in the presence of antimony ions, during which the solution behavior, synthetic product composition, and bacterial metabolism were studied. The results show that in the presence of Sb(V), Fe2+ was rapidly oxidized to Fe3+ by A. ferrooxidans and Sb(V) had no obvious effect on the biooxidation of Fe2+ under the current experimental conditions. The presence of Sb(III) inhibited bacterial growth and Fe2+ oxidation. For the group with Sb(III), products with amorphous phases were formed 72 hr later, which were mainly ferrous sulfate and pentavalent antimony oxide, and the amorphous precursor was finally transformed into a more stable crystal phase. For the group with Sb(V), the morphology and structure of jarosite were changed in comparison with those without Sb. The biomineralization process was accompanied by the removal of 94% Sb(V) to form jarosite containing the Fe-Sb-O complex. Comparative transcriptome analysis shows differential effects of Sb(III) and Sb(V) on bacterial metabolism. The expression levels of functional genes related to cell components were much more downregulated for the group with Sb(III) but much more regulated for that with Sb(V). Notably, cytochrome c and nitrogen fixation-relevant genes for the A.f_Fe2+_Sb(III) group were enhanced significantly, indicating their role in Sb(III) resistance. This study is of great value for the development of antimony pollution control and remediation technology.
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Acidithiobacillus , Antimonio , Sulfatos , Acidithiobacillus/metabolismo , Acidithiobacillus/efectos de los fármacos , Sulfatos/metabolismo , Compuestos Férricos , Oxidación-Reducción , Minería , Hierro/metabolismoRESUMEN
To reveal the mechanism of Shenkang injection (SKI) in the treatment of chronic renal failure, and verify the key pathway. In this work, an untargeted metabolomics approach was performed by LC-MS coupled with multivariate statistical analysis to provide new insights into therapeutic mechanism of SKI. Hematoxylin-eosin (H&E) Staining and Immunohistochemistry were used to evaluate the effects of drug treatment, Western blot was used to verify the critical pathway. Then, a total of 44 potential biomarkers of chronic renal failure (CRF) were identified and reversed regulation, including 2,8-dihydroxypurine, 5-methoxytryptophan, uric acid, acetylcarnitine, taurine, etc. Mainly concerned with arginine and proline metabolism, purine metabolism, histidine metabolism, etc. Pathological examination showed that the renal interstitium of SKI group was significantly improved, with fewer inflammatory cells and thinner vascular walls compared with the model group. Immunohistochemical results showed that the expression of α-smooth muscle actin (α-SMA) was decreased, and the expression of E-cadherin was increased in CRF model group, and the two indicators were reversed regulation in SKI injection, indicating that the degree of fibrosis was relieved. Critical signaling pathway phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) and nuclear factor-kappaB (NF-κB) protein expressions were significantly inhibited. This study was the first to employ metabolomics to elucidate the underlying mechanisms of SKI in chronic renal failure. The results would provide some support for clinical application of traditional Chinese medicines in clinic.
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Medicamentos Herbarios Chinos , Fallo Renal Crónico , Insuficiencia Renal Crónica , Humanos , Fosfatidilinositol 3-Quinasas/metabolismo , Vías Clínicas , Riñón , Fallo Renal Crónico/tratamiento farmacológico , Insuficiencia Renal Crónica/tratamiento farmacológicoRESUMEN
The biosilicification of diatoms allows for the customization of the synthesis of functionalized diatom frustules. The S active sites (-SH) on diatom frustules were created by adding the organic silicon sources tetramethoxysilane (TMOS) and (3-mercaptopropyl)trimethoxysilane (MPTMS). The mechanisms of adsorption-reduction and the indirect effects of S active sites on electrochemical performance were declared. The DBS@C-Ag-3 anode material sourced from the cultivation condition with a silicon source of TMOS:MPTMS = 3:1 shows the best comprehensive performance and delivers a discharge capacity of â¼660 mAh·g-1 after 1000 cycles at 1 A·g-1. The electrochemical performance of DBS@C-Ag anode materials is also found to be dominated by structure at high temperatures and conductivity at low temperatures. Such a diatom frustule structure with sulfhydryl functionalization is promising for anode materials, and it suggests a biological strategy for creating other electrode materials by modifying them with metals to improve electrochemical performances.
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OBJECTIVE: To explore the potential mechanism of Shenkang injection (SKI) in the treatment of chronic renal failure based on network pharmacology and molecular docking technology, and to verify the core targets and key pathways by using the renal failure model. METHODS: The active components and targets of Shenkang injection were retrieved by TCMSP database, and the disease related targets were obtained by OMIM, GeneCards and other databases. Then, the intersection was obtained, and were imported into String database for PPI analysis. After further screening of core targets, GO and KEGG analysis were performed. Autodock software was used to predict the molecular docking and binding ability of the selected active ingredients and core targets. Chronic renal failure (CRF) model was established by adenine induction in rats, and the pathological observation of renal tissues was conducted. Meanwhile, the effects of Shenkang injection and its active components on core targets and pathways of renal tissues were verified. RESULTS: The results of network pharmacology showed that the main components of Shenkang injection might be hydroxysafflor yellow A (HSYA)ãtanshinolãrheum emodinãAstragaloside IV. Through enrichment analysis of core targets, it was found that Shenkang injection may play an anti-chronic renal failure effect through PI3K-Akt signaling pathway. Molecular docking results showed that the above pharmacodynamic components had strong binding ability with the target proteins PI3K and Akt. The results of animal experiments showed that renal function indexes of Shenkang injection group and pharmacodynamic component group were significantly improved compared with model group. HE staining results showed that the pathological status of the kidney was significantly improved in SKI and pharmacodynamic component treatment groups. Immunohistochemical results showed that the renal fibrosis status was significantly reduced in SKI and pharmacodynamic component treatment groups. q-RTPCR and WB results showed that the expression levels of PI3K and Akt were significantly decreased in the treatment groups (P< 0.05). CONCLUSIONS: Shenkang injection may inhibit PI3K-Akt signaling pathway to play an anti-chronic renal failure role through the pharmacodynamic component hydroxysafflor yellow A (HSYA), tanshinol, rheum emodin, Astragaloside IV.
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Medicamentos Herbarios Chinos , Emodina , Fallo Renal Crónico , Insuficiencia Renal Crónica , Animales , Ratas , Simulación del Acoplamiento Molecular , Farmacología en Red , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Fallo Renal Crónico/tratamiento farmacológico , Insuficiencia Renal Crónica/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Medicamentos Herbarios Chinos/uso terapéuticoRESUMEN
The study aimed to explore the pharmacokinetic and pharmacodynamic alterations of the active components of Shenkang injection (i.e. hydroxy saffron yellow pigment A [HSYA], tanshinol, rheum emodin, and astragaloside IV) in rats with chronic renal failure (CRF), and establish a pharmacokinetic-pharmacodynamic model (PK-PD model) in order to provide a scientific and theoretical basis for the rational clinical use of Shenkang injection. Sprague-Dawley (SD) rats were randomly divided into a normal group, model group, and Shenkang injection group. A rat model of CRF was induced by adenine gavage and then followed by drug administration via tail vein injection. Orbital blood was collected at different timepoints and the blood concentrations of the four active components were measured by UHPLC-Q-Orbitrap HRMS. Serum levels of creatinine (Scr), urea nitrogen (BUN), and uric acid (UA) were determined using an automatic biochemical analyzer. A PK-PD model was established, and DAS 3.2.6 software was used for model fitting as well as statistical analysis. TGF-ß1 was utilized to induce normal rat kidney cells to construct a renal fibrosis model to investigate the protective effect of the pharmacological components on renal fibrosis. The pharmacokinetic analysis of hydroxy saffron yellow pigment A, tanshinol, rheum emodin, and astragaloside IV based on UHPLC-Q-Orbitrap HRMS was stable. The linear regression equations for the four active components were as follows: Y = 0.031X + 0.0091 (R2 = 0.9986) for hydroxy saffron yellow pigment A, Y = 0.0389X + 0.164 (R2 = 0.9979) for tanshinol, Y = 0.0257X + 0.0146 (R2 = 0.9973) for rheum emodin, and Y = 0.0763X + 0.0139 (R2 = 0.9993) for astragaloside IV, which indicated good linear relationships. The methodological investigation was stable, with the interday and intraday precision RSD <10%. Meanwhile, the recoveries ranged between 90% and 120%, in accordance with the requirements for in vivo analysis of drugs. Compared with the model group, the levels of Scr, BUN, and UA were significantly decreased after 20 min in the Shenkang injection group (p < 0.01). The PK-PD model showed that the four active components in the Shenkang injection group could fit well with the three effect measures (i.e. Scr, BUN, and UA), with the measured values similar to the predicted values. The cell model of renal fibrosis showed that the connective tissue growth factor and FN1 protein expression levels were significantly lower in the Shenkang injection group than those in the model group, and the cell fibrosis was improved. The established method for in vivo analysis of Shenkang injection was highly specific, with good separation of the components and simple operation. The total statistical moment could well integrate the pharmacokinetic parameters of the four active components. After treatment with Shenkang injection, all indexes in the administered group improved and showed significant inhibition of renal cell fibrosis in vitro. This study could provide scientific reference ideas for the clinical rational use of traditional Chinese medicine.
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Medicamentos Herbarios Chinos , Emodina , Fallo Renal Crónico , Insuficiencia Renal Crónica , Ratas , Animales , Emodina/farmacología , Ratas Sprague-Dawley , Riñón , Fallo Renal Crónico/tratamiento farmacológico , Fallo Renal Crónico/patología , Medicamentos Herbarios Chinos/farmacología , FibrosisRESUMEN
Silica is regarded as a promising anode material for lithium-ion batteries (LIBs) because of its high theoretical capacity. However, large volume variation and poor electrical conductivity are limiting factors for the development of SiO2 anode materials. To solve this problem, combining SiO2 with a conductive phase and designing hollow porous structures are effective ways. In this work, The Co(II)-EDTA chelate on the surface of diatom biosilica (DBS) frustules and obtained DBS@C-Co composites decorated with Co nanoparticles by calcination without a reducing atmosphere is first precipitated. The unique three-dimensional structure of diatom frustules provides enough space for the volume change of silica during lithiation/delithiation. Co nanoparticles effectively improve the electrical conductivity and electrochemical activity of silica. Through the synergistic effect of the hollow porous structure, carbon layer and Co nanoparticles, the DBS@C-Co-60 composite delivers a high reversible capacity of >620 mAh g-1 at 100 mA g-1 after 270 cycles. This study provides a new method for the synthesis of metal/silica composites and an opportunity for the development of natural resources as advanced active materials for LIBs.
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Bioleaching processes or microbially mediated iron/sulfur redox processes in acid mine drainage (AMD) result in mineral dissolution and transformation, the release of mercury and other heavy metal ions, and changes in the occurrence forms and concentration of mercury. However, pertinent studies on these processes are scarce. Therefore, in this work, the Fe/S redox-coupled mercury transformation mediated by Acidithiobacillus ferrooxidans ATCC 23270 under aerobic and/or anaerobic conditions was studied by combining analyses of solution behavior (pH, redox potential, and Fe/S/Hg ion concentrations), the surface morphology and elemental composition of the solid substrate residue, the Fe/S/Hg speciation transformation, and bacterial transcriptomics. It was found that: (1) the presence of Hg2+ significantly inhibited the apparent iron/sulfur redox process; (2) the addition of Hg2+ caused a significant change in the composition of bacterial surface compounds and elements such as C, N, S, and Fe; (3) Hg mainly occurred in the form of Hg0, HgS, and HgSO4 in the solid substrate residues; and (4) the expression of mercury-resistant genes was higher in earlier stages of growth than in the later stages of growth. The results indicate that the addition of Hg2+ significantly affected the iron/sulfur redox process mediated by A. ferrooxidans ATCC 23270 under aerobic, anaerobic, and coupled aerobic-anaerobic conditions, which further promoted Hg transformation. This work is of great significance for the treatment and remediation of mercury pollution in heavy metal-polluted areas.
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The domoic acid (DA) produced by certain species of the marine pennate diatom genus Pseudo-nitzschia is highly neurotoxic and can induce nerve excitability and neurotoxicity by binding with ionotropic glutamate receptors, causing amnesic shellfish poisoning in humans who consume seafood contaminated with DA. In recent years, poisoning of humans caused by DA has occurred around the world, which has attracted increasing attention, and studies on DA production by Pseudo-nitzschia have become the hotpot. This article reviews the progress in the biosynthesis of DA by the typical diatom Pseudo-nitzschia, in which the metabolic pathway of the biosynthesis of DA and its precursors, i.e., geranyl pyrophosphate and L-glutamate, and the various environmental factors affecting DA production including temperature, light intensity, nutrients, trace metals, and alien bacteria are discussed. The detection methods of DA (including bioassays, enzyme linked immunosorbent assays, high performance liquid chromatography, capillary electrophoresis and biosensors), as well as the morphology and toxigenicity of Pseudo-nitzschia are also presented.
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Diatomeas , Síndromes de Neurotoxicidad , Intoxicación por Mariscos , Humanos , Diatomeas/metabolismo , Ácido Kaínico/análisis , Ácido Kaínico/metabolismo , Redes y Vías MetabólicasRESUMEN
Red mud (RM) is a highly alkaline polymetallic waste generated via the Bayer process during alumina production. It contains metals that are critical for a sustainable development of modern society. Due to a shortage of global resources of many metals, efficient large-scale processing of RM has been receiving increasing attention from both researchers and industry. This study investigated the solubilization of metals from RM, together with RM dealkalization, via sulfur (S0) oxidation catalyzed by the moderately thermophilic bacterium Sulfobacillus thermosulfidooxidans. Optimization of the bioleaching process was conducted in shake flasks and 5-L bioreactors, with varying S0:RM mass ratios and aeration rates. The ICP analysis was used to monitor the concentrations of dissolved elements from RM, and solid residues were analyzed for surface morphology, phase composition, and Na distribution using the SEM, XRD, and STXM techniques, respectively. The results show that highest metal recoveries (89% of Al, 84% of Ce, and 91% of Y) were achieved with the S0:RM mass ratio of 2:1 and aeration rate of 1 L/min. Additionally, effective dealkalization of RM was achieved under the above conditions, based on the high rates (>95%) of Na, K, and Ca dissolution. This study proves the feasibility of using bacterially catalyzed S0 oxidation to simultaneously dealkalize RM and efficiently extract valuable metals from the amassing industrial waste.
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A novel sinter method using ZnO as the activator instead of the conventional Na2CO3/CaCO3, (NH4)2SO4, and K2S2O7 was developed to achieve efficient sequential extraction of rare earth elements (REEs), alumina (Al), and silica (Si) from coal fly ash (CFA). Up to 93.3% Si, 87.1% REEs (70.7% Ce, 82.5% La, 83.2% Gd, 87.1% Nd, 62.3% Dy, and 81.7% Y), and 92.9% Al were extracted from CFA, respectively. Moreover, 93.1% of the ZnO activator was efficiently recycled, and the yield of red mud was only 14.9%. X-ray diffraction (XRD) and X-ray absorption near edge structure (XANES) results showed that the speciation transformation of Al/Si during CFA/ZnO roasting was as follows: mullite, quartz, amorphous Al2O3, and SiO2 â Zn0.75Al1.5Si1.5O6, kyanite and willemite â gahnite and quartz/cristobalite solid solutions. The change in the REEs occurrence mode hinted at the migration of most REEs in aluminosilicates forms with Si during roasting, and disassociation with Si into the acid-soluble form after alkali leaching. These results indicate that the coupling of Al-Si-REE in CF was broken by this ZnO sinter method, promoting the sequential and efficient extraction of REEs, Al, and Si from CFA. This study provides a green and efficient strategy for element recovery from CFA, substantially reducing residues and favoring REEs concentration.
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Oxidative dissolution of stibnite (Sb2S3), one of the most prevalent geochemical processes for antimony (Sb) release, can be promoted by Sb-oxidizing microbes, which were studied under alkaline and neutral conditions but rarely under acidic conditions. This work is dedicated to unraveling the enhancement mechanism of stibnite dissolution by typical acidophile Acidithiobacillus ferrooxidans under extremely acidic conditions. The results of solution behavior showed that the dissolution of Sb2S3 was significantly enhanced by A. ferrooxidans, with lower pH and higher redox potential values and higher [Sb(III)] and [Sb(V)] than the sterile control. The surface morphology results showed that the cells adsorbed onto the mineral surface and formed biofilms. Much more filamentous secondary minerals were formed for the case with A. ferrooxidans. Further mineral phase compositions and Sb/S speciation transformation analyses showed that more secondary products Sb2O3/SbO2-, Sb2O5/SbO3-, SO42-, as well as intermediates, such as S0, S2O32- were formed for the biotic case, indicating that the dissolution of Sb2S3 and the Sb/S speciation transformation was promoted by A. ferrooxidans. These results were further clarified by the comparative transcriptome analysis. This work demonstrated that through the interaction with Sb2S3, A. ferrooxidans promotes S/Sb oxidation, so as to enhance S/Sb transformation and thus the dissolution of Sb2S3.
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Acidithiobacillus , Antimonio/química , Minerales/química , Oxidación-Reducción , SolubilidadRESUMEN
Red mud (RM) as waste of industrial aluminum production is piling up in huge ponds. RM could be a cost-effective adsorbent for heavy metals, but adsorption is vulnerable to pH changes, metal ions speciation and the occurrence of iron bearing minerals. In this study, the precipitation and elemental speciation transformation relevant to arsenic fate in responding to the addition of RM during arsenopyrite bio-oxidation by Sulfobacillus thermosulfidooxidans was investigated. The results show that the addition of RM significantly changed the arsenic precipitation and the solution chemistry and thus affected the arsenopyrite bio-oxidation and arsenic fate. An addition of a small amount (≤ 4 g/L) of RM substantially promoted arsenopyrite bio-oxidation with formation of SiO2 @ (As, Fe, Al, Si) spherical nanoparticles that can enhance the stability of the immobilized arsenic. The SiO2-based spherical nanoparticles precipitate was mainly composed of jarosites, amorphous ferric arsenate and crystalline scorodite, and its formation were controlled by Fe3+ concentration and solution pH. An addition of increased amount of RM (≥ 6 g/L) resulted in a significant increase of the solution pH and a decrease in the Fe2+ bio-oxidation activity, and spherical nanoparticles were not formed. Consequently, the dissolution of arsenopyrite was inhibited and the release of arsenic was blocked. This study suggests the applicability of RM in mitigation of arsenic pollution from bio-oxidation of As-bearing sulfide minerals.
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Arsénico , Arsenicales , Clostridiales , Concentración de Iones de Hidrógeno , Hierro , Compuestos de Hierro , Minerales , Oxidación-Reducción , Dióxido de Silicio , SulfurosRESUMEN
Arsenic often coexists with metal sulfide minerals and occurs in different speciation and different toxicity in responding to Fe/S biooxidation. The differential inhibitive effects and fates of As(III) and As(V) during biooxidations of elemental sulfur (S0), ferrous ions (Fe2+) and pyrite (FeS2) by Sulfobacillus thermosulfidooxidans were studied. The results revealed that the arsenic species hardly changed for the biooxidation of S0, but dramatically changed for the biooxidation of Fe2+ and FeS2. Different transformation degree between As(III) and As(V) occurred for biooxidation of FeS2 in the presence of arsenic, where about 72% of As(III) was transformed to As(V) for the group with As(III) added, and 16% of As(V) was transformed to As(III) for that with As(V) added. Both formation and dissolution of amorphous ferric arsenate occurred during biooxidation of FeS2 with the addition of As(III) or As(V) and for the group grown on Fe2+ with added As(V), which were controlled by the changes of Fe/As molar ratio and pH value in the solution. Jarosite was detected for the group grown on Fe2+ and could adsorb As(III) and As(V). The inhibitive effects of As(V) were higher than As(III) when the strain grew on FeS2, which was contrary to those when the strain grew on S0 and Fe2+. The above results signify that the fates and inhibitive effects of arsenic are much related to each other, and such a relationship is significantly affected by the utilization of Fe/S energy substrates by the sulfur- and ferrous-oxidizing microorganisms.
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Arsénico , Clostridiales , Compuestos Férricos , Minerales , Oxidación-Reducción , AzufreRESUMEN
It is well known that speciation transformations of As(III) vs. As(V) in acid mine drainage (AMD) are mainly driven by microbially mediated redox reactions of Fe and S. However, these processes are rarely investigated. In this study, columns containing mine water were inoculated with two typical acidophilic Fe/S-oxidizing/reducing bacteria [the chemoautotrophic Acidithiobacillus (At.) ferrooxidans and the heterotrophic Acidiphilium (Aph.) acidophilum], and three typical energy substrates (Fe2+, S0, and glucose) and two concentrations of As(III) (2.0 and 4.5 mM) were added. The correlation between Fe/S/As speciation transformation and bacterial depth distribution at three different depths, i.e., 15, 55, and 105 cm from the top of the columns, was comparatively investigated. The results show that the cell growth at the top and in the middle of the columns was much more significantly inhibited by the additions of As(III) than at the bottom, where the cell growth was promoted even on days 24-44. At. ferrooxidans dominated over Aph. acidophilum in most samples collected from the three depths, but the elevated proportions of Aph. acidophilum were observed in the top and bottom column samples when 4.5 mM As(III) was added. Fe2+ bio-oxidation and Fe3+ reduction coupled to As(III) oxidation occurred for all three column depths. At the column top surfaces, jarosites were formed, and the addition of As(III) could lead to the formation of the amorphous FeAsO4â 2H2O. Furthermore, the higher As(III) concentration could inhibit Fe2+ bio-oxidation and the formation of FeAsO4â 2H2O and jarosites. S oxidation coupled to Fe3+ reduction occurred at the bottom of the columns, with the formations of FeAsO4â 2H2O precipitate and S intermediates. The formed FeAsO4â 2H2O and jarosites at the top and bottom of the columns could adsorb to and coprecipitate with As(III) and As(V), resulting in the transfer of As from solution to solid phases, thus further affecting As speciation transformation. The distribution difference of Fe/S energy substrates could apparently affect Fe/S/As speciation transformation and bacterial depth distribution between the top and bottom of the water columns. These findings are valuable for elucidating As fate and toxicity mediated by microbially driven Fe/S redox in AMD environments.
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Phenomics is originally a biological concept. In the most recent years, the studies of plant and human phenomics have started, and show a strong momentum and trend of development. In this paper, based on the related research on bioleaching/acid mine drainage (AMD), we put forward the relevant concepts and methodology of phenomics of microbe-mineral interaction (MMI) in bioleaching/AMD environments. It refers to the systematic study on phenotypes of MMI on both levels of microbiome and mineralome under various environmental conditions, by which it gives the relationship between microbial/mineral genome and phenome of MMI responding to the varying environmental conditions. The pertinent methodology is of mainly (meta)-omics, synchrotron radiation-based techniques and supercomputing-based density function theory (DFT) calculation.
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Minería , Fenómica , Ácidos , Humanos , MineralesRESUMEN
Abiotic iron monosulfide (FeS) has attracted growing interests in dechlorinating trichloroethylene (TCE) in anoxic groundwater, but it is still unclear how biogenic FeS affects the dechlorination and thus the cytotoxity of TCE. In this work, a biogenic FeS was synthesized by Shewanella oneidensis MR-1 with addition of ferrihydrite and S0, and it was used for dechlorination of TCE in alkaline environment and the de-cytotoxicity was evaluated by the growth of Synechocystis sp. PCC6803. The results show that the biogenic FeS was of mackinawite, with a loose flower-like mosaic structure. The dechlorination of TCE by the biogenic FeS was accelerated by 6 times than that by abiotic FeS. TCE was dechlorinated mainly by hydrogenolysis to form dichloroethane (C2H2Cl2), vinyl chloride (C2H3Cl), and finally ethylene, accompanied with transformation of both Fe2+ to Fe3+ and monosulfide to disulfide and polysulfide on the biogenic FeS surface. The concentration for 50% of maximal inhibition effect (EC50) of TCE to Synechocystis was 486 mg/L and the inhibition to Synechocystis under the EC50 was relieved more significantly on addition of the biogenic FeS than that of abiotic FeS. These results indicate that the biogenic FeS promoted the dechlorination and thus de-cytotoxity of TCE.
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Compuestos Ferrosos/metabolismo , Shewanella/metabolismo , Tricloroetileno/metabolismo , Contaminantes Químicos del Agua/metabolismoRESUMEN
Statins, a class of drugs that can effectively remove cholesterol from serum, are used to regulate plasma total cholesterol and reduce the risk of cardiovascular diseases, but it is still unclear whether the drug are modulated by gut microbiota or the structures of gut microbiota are shaped by statins. We investigated the interactions between statins and the human gut microbiota during the in vitro fermentation process by 16S rRNA gene sequencing, gas chromatography (GC), and high-performance liquid chromatography (HPLC) analyses. The presence of fluvastatin (FLU2) specifically promoted the growth of Escherichia/Shigella, Ruminococcaceae UCG 014, and Sutterella. However, the composition of the gut bacterial microbiota remained relatively static in samples treated with rosuvastatin (ROS), simvastatin (SIM), and atorvastatin (ATO). The PICRUSt program predicted moderate differences in the functional categories related to the biosynthesis of other secondary metabolites, cellular processes and signaling, and signal transduction in the FLU2 fermentation samples. Our study revealed substantial variation in the structure and function of microbiomes from the FLU2-treated samples. In addition, short-chain fatty acids (SCFAs) were also significantly decreased in FLU2-treated samples compared with the samples treated with other stains. Statins can be degraded by the human gut microbiota in vitro, and the degradation rate was approximately 7%-30% and 19%-48% after fermentation was allowed to proceed for 24 h and 48 h, respectively. Generally, FLU2 could largely shape the composition and function of human gut microbiota, which resulted in changes in the production of SCFAs. In turn, all statins could be degraded or modified by the gut microbiota. Our study paves the way for elucidating statin-gut microbiota interactions in vitro towards the improvement of the host health and personalized medicine.
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Bacterias/efectos de los fármacos , Microbioma Gastrointestinal/efectos de los fármacos , Microbiota/efectos de los fármacos , Adolescente , Adulto , Bacterias/genética , Enfermedades Cardiovasculares/tratamiento farmacológico , Ácidos Grasos Volátiles/genética , Heces/microbiología , Femenino , Fermentación/efectos de los fármacos , Fermentación/genética , Microbioma Gastrointestinal/genética , Humanos , Inhibidores de Hidroximetilglutaril-CoA Reductasas/uso terapéutico , Masculino , Microbiota/genética , ARN Ribosómico 16S/genética , Adulto JovenRESUMEN
The bio-oxidative dissolution of arsenopyrite, the most severe arsenic contamination source, can be mediated by organic substances, but pertinent studies on this subject are scarce. In this study, the bio-oxidative dissolution of arsenopyrite by Sulfobacillus thermosulfidooxidans and arsenic immobilization were evaluated in the presence of humic acid (HA). The mineral dissolution was monitored through analyses of the parameters in solution, phase and element speciation transformations on the mineral surface, and arsenic immobilization on the surfaces of cells and jarosites-HA. The results show that the presence of HA enhances the dissolution of arsenopyrite, e.g., 7.1% of arsenopyrite was in the residue after 12 d of bio-oxidation compared to 19.3% in the absence of HA. Meanwhile, the presence of HA led to changes of the fates of As and Fe and no accumulation of elemental sulfur (S0) or ferric arsenate on the mineral surface. Moreover, a flocculent porous structure was formed on the surfaces of both microbial cells and jarosites, on which a large amount of arsenic was adsorbed. These results clearly indicate that HA can simultaneously promote the dissolution of arsenopyrite and arsenic immobilization, which may be significant for bioleaching of arsenopyrite-bearing contaminated sites.
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Arseniatos/análisis , Arsenicales/química , Arsenitos/análisis , Clostridiales/metabolismo , Sustancias Húmicas/análisis , Compuestos de Hierro/química , Minerales/química , Sulfuros/química , Arseniatos/metabolismo , Arsenicales/metabolismo , Arsenitos/metabolismo , Biodegradación Ambiental , Compuestos Férricos/química , Compuestos de Hierro/metabolismo , Minerales/metabolismo , Modelos Teóricos , Oxidación-Reducción , Solubilidad , Sulfatos/química , Sulfuros/metabolismo , Propiedades de SuperficieRESUMEN
For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN). The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells. The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides. The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values. These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.