The combination of aerobic and microaerobic promote hydrolysis and acidification of rice straw and pig manure: Balance of insoluble and soluble substrate.

Separated hydrolysis and acidification is an effective pretreatment method for anaerobic digestion of lignocellulose. However, excess consumption of soluble substrates remains a problem. Rice straw and pig manure were used as substrates with biogas slurry as the inoculum, combined with aerobic and microaerobic conditions in the 14-day hydrolysis and acidification. Aeration can significantly accelerate volatile solid degradation (38.25%), especially the lignocellulose. Soluble chemical oxygen demand (29157 mg/L) and volatile fatty acids (13219 mg/L) of the group with 4 days aerobic treatment, reached their peaks on day 5, obtaining a balanced insoluble substrate degradation and soluble substrate consumption. Candida, Lactobacillus, Bifidobacterium, and Acetobacter were enriched at the balanced point for positive contribution to the degradation of the insoluble substrate and the generation of soluble substrate. This study not only reveals the balance between degradation and consumption, but also provides new insight into biogas slurry recycling and anaerobic digestion precursor substrate production.

Coupling suspect and non-target analytical methods for screening organic contaminants of concern in agricultural & urban impacted waters: Optimization and application.

Long-term exposure of contaminants to emerging concern (CECs) may pose risks to human health and ecosystems, even at low concentrations. Rivers impacted by both agricultural and urban activities experience distinctive environmental pressures due to receiving wastewaters that contain complex organics and their transformation products (TPs). In this study, we developed a regional database composed of 1200 CECs of high concern in Guangxi (South China). Further, we optimized a comprehensive analytical method for simultaneously screening for CECs and their TPs. The optimized screening method was applied to surface waters sampled from 10 different cross sections of a river that is impacted by both agricultural and urban activities. The best results of method optimization were achieved when the screening detection limit (SDL) ranged from 0.05 to 2 ng L-1, and over 90% of the analytes had acceptable recovery rates ranging between 64.7% and 95.6% (RSD < 11%). Of the 1200 CECs contained in the regional database, 168 were detected in at least one sampling site of the studied river via suspect screening, and among them, 36 contaminants were found at all sampling sites. Also, 58 additional contaminants and 39 TPs were tentatively identified via non-target screening, among which 4 TPs were reported for the first time in the aquatic environment. Triazine herbicides and their TPs were identified at most of the sampling sites, with ametryn and atrazine posing relatively high risks in the river ecosystems. Furthermore, 31 known analytes were selected as standards in order to confirm the combined screening method; one false positive occurred in the non-target screening method. According to these results, the suspect screening strategy provides valuable confirmation for the identification of a wide range of CECs in water, while non-target screening can provide a reference for researchers and supplement the regional database, particularly in the study of TPs.

Improving the efficiency of anaerobic digestion: Domesticated paddy soil microbes enhance the hydrolytic acidification of rice straw and pig manure.

Improving the efficiency of hydrolytic acidification is critical for methane production from agricultural waste. This study is the first to apply domesticated paddy soil microbes to (DPSM) enhance the hydrolytic acidification of rice straw (RS) and pig manure (PM) to obtain acidizing fluid for anaerobic digestion (AD). At a substrate concentration of 20%, the inoculation of an RS-PM mixture (1:3) with 35% DPSM degraded the volatile solids by 48.1% and yielded 6.8 g/L of volatile fatty acids and 4.7 g/L of acetic acid after seven days of hydrolytic acidification. After 10 days of subsequent AD, the cumulative methane production of the acidizing fluid was 304.96 mL/g COD, similar (P ï¼ž 0.05) to the control (318.27 mL/g COD). However, the methane production time decreased by 43.4% (from 30 to 17 days), thereby improving the AD efficiency. Inoculation with DPSM is therefore an effective pre-treatment for agricultural waste for methane production.

Assessment of Potential Nitrite Safety Risk of Leafy Vegetables after Domestic Cooking.

Improper cultivation can easily cause excessive nitrate accumulation in leafy vegetables, and the cooking processes used to prepare them can upset their nitrate/antioxidant balance, affecting their potential nitrite safety risk (PNSR). We investigated the impacts stir-frying, steaming, microwaving, and boiling on the nitrate, nitrite, and antioxidant capacity in water spinach and cabbage, and observed the impacts of storage duration on the PNSR. The antioxidant/in vivo nitrite ratio (A/N) was used to evaluate the nitrite risks in the cooked vegetables. Boiling achieved the highest A/N ratio (1.57) for water spinach, reducing the nitrate content by 25% without significantly affecting the antioxidant capacity. Stir-frying achieved the highest A/N ratio (6.55) for cabbage, increasing the antioxidant capacity by 140% without significantly affecting the nitrate content. Furthermore, it was found that the storage periods for boiled water spinach and stir-fried cabbage should not exceed 12 h and 24 h, respectively. Appropriate cooking methods and limited storage times are thus required for leafy vegetable to prevent adverse health effects.

Green, microwave-assisted synthesis of silver nanoparticles using bamboo hemicelluloses and glucose in an aqueous medium.

A green, straightforward, microwave-assisted method of synthesizing silver nanoparticles in an aqueous medium was developed using bamboo hemicelluloses as stabilizer and glucose as reducer. The effects of irradiation time as well as initial concentrations of hemicelluloses, glucose, and AgNO(3) on the silver nanoparticle formation were studied. The silver nanoparticles were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results indicated the formation of spherical, nanometer-sized particles. The reaction parameters significantly affected the formation rate, size and distribution of the silver nanoparticles. The average particle size was 8.3-14.8 nm based on TEM analysis. XRD analysis revealed that the particles calcined at 300 °C were face-centered cubic. XPS characterization showed that silver Ag(0) coexisted with silver Ag(I). The synthesis process of silver nanoparticles was rapid and eco-friendly.