Unexpected increase in microalgal removal of doxylamine induced by bicarbonate addition: synergistic chem-/bio-degradation mechanisms.

Microalgae-based approaches serve as promising methods for the remediation of pharmaceutical contaminants (PCs) compared to conventional wastewater treatment processes. However, how to decrease hydraulic retention times of the microalgal system currently has been one of the main bottlenecks. This study constructed an unexpected synergistic extra-chemical/intra-biological degradation system by adding 5.95 mM bicarbonate to the microalgal system, which achieved complete removal (100%) of a representative PC, doxylamine (DOX) in 96 h, compared to that 192 h in the control. Removal capacities and mass balance analyses demonstrated that biodegradation rate per unit microalgal density was significantly increased by 207%. Further analyses using transcriptomic, enzymatic inhibiting tests, and high-resolution mass spectrometry revealed that after addition of bicarbonate for metabolism of DOX, a hydrolase (CYP97C1) and a primary amine oxidase (TynA) can transform DOX into doxylamine N-oxide and an intermediate (C15H17NO2) with a m/z of 244.1335. Meanwhile, bicarbonate reacted with microalgae-excreted hydrogen peroxide to form more oxidative radicals such as superoxide and hydroxyl radicals extracellularly, which promised the extracellular degradation of DOX according to the oxidative radical inhibiting tests. Further investigation showed addiing bicarbonate to the microalgal system improved the removal rate of 17 PCs by up to 500.8%. Therefore, this study not only developed an approach to enhance treatment efficiencies of diverse PCs by microalgae within a shorter time, but also carried unique mechanistic insights into the underlying principles.

Functional characterization of a novel Chlamydomonas reinhardtii hydrolase involved in biotransformation of chloramphenicol.

Microalgae-based biotechnology is one of the most promising alternatives to conventional methods for the removal of antibiotic contaminants from diverse water matrices. However, current knowledge regarding the biochemical mechanisms and catabolic enzymes involved in microalgal biodegradation of antibiotics is scant, which limits the development of enhancement strategies to increase their engineering feasibility. In this study, we investigated the removal dynamics of amphenicols (chloramphenicol, thiamphenicol, and florfenicol), which are widely used in aquaculture, by Chlamydomonas reinhardtii under different growth modes (autotrophy, heterotrophy, and mixotrophy). We found C. reinhardtii removed >92 % chloramphenicol (CLP) in mixotrophic conditions. Intriguingly, gamma-glutamyl hydrolase (GGH) in C. reinhardtii was most significantly upregulated according to the comparative proteomics, and we demonstrated that GGH can directly bind to CLP at the Pro77 site to induce acetylation of the hydroxyl group at C3 position, which generated CLP 3-acetate. This identified role of microalgal GGH is mechanistically distinct from that of animal counterparts. Our results provide a valuable enzyme toolbox for biocatalysis and reveal a new enzymatic function of microalgal GGH. As proof of concept, we also analyzed the occurrence of these three amphenicols and their degradation intermediate worldwide, which showed a frequent distribution of the investigated chemicals at a global scale. This study describes a novel catalytic enzyme to improve the engineering feasibility of microalgae-based biotechnologies. It also raises issues regarding the different microalgal enzymatic transformations of emerging contaminants because these enzymes might function differently from their counterparts in animals.

Recovery mechanism of a microalgal species, Chlorella sp. from toxicity of doxylamine: Physiological and biochemical changes, and transcriptomics.

Ubiquitous distribution of pharmaceutical contaminants in environment has caused unexpected adverse effects on ecological organisms; however, how microorganisms recover from their toxicities remains largely unknown. In this study, we comprehensively investigated the effect of a representative pollutant, doxylamine (DOX) on a freshwater microalgal species, Chlorella sp. by analyzing the growth patterns, biochemical changes (total chlorophyll, carotenoid, carbohydrate, protein, and antioxidant enzymes), and transcriptomics. We found toxicity of DOX on Chlorella sp. was mainly caused by disrupting synthesis of ribosomes in nucleolus, and r/t RNA binding and processing. Intriguingly, additional bicarbonate enhanced the toxicity of DOX with decreasing the half-maximum effective concentrations from 15.34 mg L-1 to 4.63 mg L-1, which can be caused by inhibiting fatty acid oxidation and amino acid metabolism. Microalgal cells can recover from this stress via upregulating antioxidant enzymatic activities to neutralize oxidative stresses, and photosynthetic pathways and nitrogen metabolism to supply more energies and cellular signaling molecules. This study extended our understanding on how microalgae can recover from chemical toxicity, and also emphasized the effect of environmental factors on the toxicity of these contaminants on aquatic microorganisms.

Bisphenols can promote antibiotic resistance by inducing metabolic adaptations and natural transformation.

Whether bisphenols, as plasticizers, can influence bacterial uptake of antibiotic resistance genes (ARGs) in natural environment, as well as the underlying mechanism remains largely unknown. Our results showed that four commonly used bisphenols (bisphenol A, S, F, and AF) at their environmental relative concentrations can significantly promote transmission of ARGs by 2.97-3.56 times in Acinetobacter baylyi ADP1. Intriguingly, we observed ADP1 acquired resistance by integrating plasmids uptake and cellular metabolic adaptations other than through reactive oxygen species mediated pathway. Metabolic adaptations including upregulation of capsules polysaccharide biosynthesis and intracellularly metabolic enzymes, which enabled formation of thicker capsules for capturing free plasmids, and degradation of accumulated compounds. Simultaneously, genes encoding DNA uptake and translocation machinery were incorporated to enhance natural transformation of antibiotic resistance carrying plasmids. We further exposed aquatic fish to bisphenols for 120 days to monitor their long-term effects in aquatic environment, which showed that intestinal bacteria communities were dominated by a drug resistant microbiome. Our study provides new insight into the mechanism of enhanced natural transformation of ARGs by bisphenols, and highlights the investigations for unexpectedly-elevated antibiotic-resistant risks by structurally related environmental chemicals.

Occurrence of antibiotics in reclaimed water, and their uptake dynamics, phytotoxicity, and metabolic fate in Lolium perenne L.

Reclaimed water (RW) has been extensively used for irrigation in agriculture, yet the occurrence of antibiotics in real RW, and their toxicity, uptake dynamics and metabolic fate still needs comprehensive exploration. In this study, we investigated the residual concentrations of nineteen antibiotics in the RW from four wastewater treatment plants, and determined their toxicity on plant at environment-relevant concentration. Total found concentrations of these antibiotics ranged from 623.66 ng L-1 to 1536.96 ng L-1, which decreased 10.3 and 19.4 % of roots' length and weight. Uptake dynamics analysis of the most hazardous antibiotic, norfloxacin (NFX) showed increasing amounts in the roots and leaves up to 3087.71 µg g-1. Ryegrass also can remove >80 % of 100 µg L-1 NFX being achieved by biodegradation through ring cleavage, decarboxylation, defluorination, hydrogenation, methylation and oxidation. Toxicity assessment of the identified byproducts showed their more toxic effect on fish, daphnia and algae. This study extended our understanding of the fate of antibiotics in plants during irrigation with reclaimed water, and emphasized its safety and pollutants' biomagnification concerns.

Dissemination of sulfonamide resistance genes in digester microbiome during anaerobic digestion of food waste leachate.

The preeminence of sulfonamide drug resistance genes in food waste (FW) and the increased utilization of high-strength organic FW in anaerobic digestion (AD) to enhance methane production have raised severe public health concerns in wastewater treatment plants worldwide. In this regard, the dissemination patterns of different sulfonamide resistance genes (sul1 and sul2) and their impact on the digester core microbiota during AD of FW leachate (FWL) were evaluated. The presence of various sulfonamide antibiotics (SAs) in FWL digesters improved the final methane yield by 37 % during AD compared with FWL digesters without SAs. Microbial population shifts towards hydrolytic, acidogenic, and acetogenic bacteria in the phyla Actinobacteriota, Bacteroidota, Chloroflexi, Firmicutes, Proteobacteria, and Synergistota occurred due to SA induced substrate digestion and absorption through active transport; butanoate, propanoate, and pyruvate metabolism; glycolysis; gluconeogenesis; the citrate cycle; and pentose phosphate pathway. The initial dominance of Methanosaeta (89-96 %) declined to 47-53 % as AD progressed and shifted towards Methanosarcina (40 %) in digesters with the highest SA concentrations at the end of AD. Dissemination of sul1 depended on class 1 integron gene (intl1)-based horizontal gene transfer to pathogenic members of Chloroflexi, Firmicutes, and Patescibacteria, whereas sul2 was transmitted to Synergistota independent of intl1. Low susceptibility and ability to utilize SAs during methanogenesis shielded methanogenic archaea against selection pressure, thus preventing them from interacting with sul or intl1 genes, thereby minimizing the risk of antibiotic resistance development. The observed emergence of cationic antimicrobial peptide, vancomycin, and ß-lactam resistance in the core microbiota during AD of FWL in the presence of SAs suggests that multidrug resistance caused by bacterial transformation could lead to an increase in the environmental resistome through wastewater sludge treatment.

Bisphenols induce cardiotoxicity in zebrafish embryos: Role of the thyroid hormone receptor pathway.

Bisphenols are frequently found in the environment and have been of emerging concern because of their adverse effects on aquatic animals and humans. In this study, we demonstrated that bisphenol A, S, and F (BPA, BPS, BPF) at environmental concentrations induced cardiotoxicity in zebrafish embryos. BPA decreased heart rate at 96 hpf (hours post fertilization) and increased the distance between the sinus venosus (SV) and bulbus arteriosus (BA), in zebrafish. BPF promoted heart pumping and stroke volume, shortened the SV-BAdistance, and increased body weight. Furthermore, we found that BPA increased the expression of the dio3b, thrß, and myh7 genes but decreased the transcription of dio2. In contrast, BPF downregulated the expression of myh7 but upregulated that of thrß. Molecular docking results showed that both BPA and BPF are predicted to bind tightly to the active pockets of zebrafish THRß with affinities of -4.7 and -4.77 kcal/mol, respectively. However, BPS did not significantly affect dio3b, thrß, and myh7 transcription and had a higher affinity for zebrafish THRß (-2.13 kcal/mol). These findings suggest that although BPA, BPS, and BPF have similar structures, they may induce cardiotoxicity through different molecular mechanisms involving thyroid hormone systems. This investigation provides novel insights into the potential mechanism of cardiotoxicity from the perspective of thyroid disruption and offer a cautionary role for the use of BPA substitution.

Unraveling the key driving factors involved in cometabolism enhanced aerobic degradation of tetracycline in wastewater.

Cometabolism has shown great potential in increasing the engineering feasibility of microalgae-based biotechnologies for the aerobic treatment of antibiotics-polluted wastewaters. Yet, the underlying mechanisms involved in improved microalgal performance remain unknown. In this study, we incorporated transcriptomics, gene network analysis, and enzymatic activities with cometabolic pathways of tetracycline (TC) by Chlorella pyrenoidosa to identify the key driving factors. The results demonstrated that cometabolism constructed a metabolic enzymes-photosynthetic machinery to improve the electron transport chain and activities of catalytic enzymes, which resulted in subsequent 100% removal of TC. Coupling formation dynamics of the intermediates with roles of identified metabolic enzymes, degradation of TC can be induced by de/hydroxylation, de/hydrogenation, bond-cleavage, decarboxylation, and deamination. Evaluation of 18 antibiotics' removal in reclaimed water showed cometabolism decreased the total concentrations of these antibiotics from 495.54 ng L-1 to 221.80 ng L-1. Our findings not only highlight the application potential of cometabolism in increasing engineering feasibility of microalgal degradation of antibiotics from wastewaters, but also provide the unique insights into unraveling the "black-box" of cometabolisms in aerobic biodegradation.

Transcriptomics unveiled metabolic perturbations in Desmodesmus quadricauda by sulfacetamide: Key functional genes involved in the tolerance and biodegradation process.

Antibiotic contamination in the environment has significant adverse effects on benthic microorganisms, which causes dysfunction of normal ecological processes. However, in-depth molecular mechanisms underlying the potential ecological impacts of these emerging pollutants are poorly understood. In this study, metabolic perturbations in a freshwater microalga, Desmodesmus quadricauda by sulfacetamide (SFM) were investigated using transcriptomics. The results found 28 genes in the tricarboxylic acid cycle and oxidative phosphorolysis pathways were significantly downregulated by 3.97 to 6.07, and 2.47 to 5.99 folds by 0.1 and 1 mg L-1 SFM, respectively. These results indicated that SFM disrupted the microalgal cellular activities through inhibition of energy metabolism. Whilst, the upregulated genes have been most enriched in porphyrin and chlorophyll metabolism (hemE, hemL, hemY, chlD, chlP, PAO, and CAO), and arachidonic acid metabolism (GGT1_5 and gpx). Expression of these genes was significantly upregulated by up to 3.36 times for tolerance against SFM. Moreover, the genes encoding decarboxylase, oxidoreductases, α-amylase, hydrolases, O-acetyltransferase, and lyase were upregulated by >2 folds, which can induce di/hydroxylation, decarboxylation, bond cleavage and deamination. These findings provide insights into the molecular mechanisms of the ecotoxicological effects of antibiotics on microalgae, and supply useful information for their environmental risk assessment and management.

Metabolic perturbations of Lolium perenne L. by enrofloxacin: Bioaccumulation and multistage defense system.

Plants are readily exposed to the antibiotics residues in reclaimed water indicating an urgent need to comprehensively analyze their ecotoxicological effects and fate of these emerging contaminants. Here, we unraveled the dissemination of enrofloxacin (ENR) in a pasture grass, Lolium perenne L., and identified multistage defense systems as its adaptation mechanism. Uptaken concentrations of ENR ranged from 1.28 to 246.60 µg g-1 with bioconcentration factors (BCF) upto 15.13, and translocation factors (TF) upto 0.332. The antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase were increased by upto 115%. Further transcriptomics demonstrated that differentially expressed genes (DEGs) involved in glycolysis, tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and glutathione metabolism were significantly up-regulated by 1.56-5.93, 3-7 and 1.04-6.42 times, respectively; whilst, the DEGs in nitrogen and sulfur metabolism pathways were significantly up-regulated by 1.06-5.64 and 2.64-3.54 folds. These processes can supply energy, signaling molecules, and antioxidants for detoxification of ENR in ryegrass. Such results provide understanding into fasting grass adaptability to antibiotics by enhancing the key protective pathways under organic pollutant stresses in environments.

Multiple metabolic pathways of enrofloxacin by Lolium perenne L.: Ecotoxicity, biodegradation, and key driven genes.

Contamination of fluoroquinolones (FQs) are of emerging concerns because of their adverse effects on environment and humans. This study investigated the ecotoxicological effects, biodegradation, and multiple metabolic pathways of a frequently found FQ, enrofloxacin (ENR) by ryegrass (Lolium perenne L.). Key metabolic genes for driving the metabolism of ENR have been identified using transcriptome profiling of L. perenne and gene network analysis. Toxicity of ENR on ryegrass has been evaluated according to the morphological changes, lipid peroxidation content, and antioxidant enzymatic activities. Moreover, there was 94.33%, 71.58%, 57.22%, and 55.23% removal of 1, 10, 50 and 100 mg L-1 ENR, respectively, which was mainly achieved by biodegradation according to the mass balance. A biodegradation pathway has been proposed by incorporating mass spectrums of extracted ENR intermediates with their formation dynamics. Analysis of differentially expressed genes (DEGs) and their network unraveled that the genes encoding monooxygenase, oxidative carboxylase, methyltransferase, lyase, hydroxylase, dehydrogenase, and peroxidase were the key functional genes. These enzymes can induce di/hydroxylation, decarboxylation, methylation, and bond and ring cleavage of ENR for its effective degradation. This study demonstrated that ryegrass can be used for efficient treatment of ENR polluted water and extended the understanding of the molecular mechanism of antibiotics' biodegradation in plants.

Insights on the role of periphytic biofilm in synergism with Iris pseudacorus for removing mixture of pharmaceutical contaminants from wastewater.

The potential of Iris pseudacorus and the associated periphytic biofilm for biodegradation of two common pharmaceutical contaminants (PCs) in urban wastewater was assessed individually and in consortium. An enhanced removal for sulfamethoxazole (SMX) was achieved in consortium (59%) compared to individual sets of I. pseudacorus (50%) and periphytic biofilm (7%) at concentration of 5 mg L-1. Conversely, individual sets of periphytic biofilm (77%) outperformed removal of doxylamine succinate (DOX) compared to individual sets of I. pseudacorus (59%) and consortium (67%) at concentration of 1 mg L-1. Enhanced relative abundance of microflora containing microalgae (Sellaphora, Achnanthidium), rhizobacteria (Acidibacter, Azoarcus, Thioalkalivibrio), and fungi (Serendipita) in periphytic biofilm was observed after treatment. SMX treatment for five days elevated cytochrome P450 enzymes' expressions, including aniline hydroxylase (48%) and aminopyrine N-demethylase (54%) in the periphytic biofilm. Nevertheless, I. pseudacorus showed 175% elevation of aniline hydroxylase along with other biotransformation enzymes, such as peroxidase (629%), glutathione S-transferase (514%), and dichloroindophenol reductase (840%). A floating bed phytoreactor planted with I. pseudacorus and the periphytic biofilm consortium removed 67% SMX and 72% DOX in secondary wastewater effluent. Thus, the implementation of this strategy in constructed wetland-based treatment could be beneficial for managing effluents containing PCs.

Unraveling the mechanism of long-term bisphenol S exposure disrupted ovarian lipids metabolism, oocytes maturation, and offspring development of zebrafish.

Bisphenol S (BPS) acts as a xenoestrogen and disturbs the female reproductive system; however, the underlying mechanism has not been elucidated. In this study, the effect of chronic BPS exposure (1 µg/L and 100 µg/L) on ovarian lipid metabolism in zebrafish was investigated to determine its influence on adult reproductive capacity and offspring development. The results showed that long-term (240 days) exposure to BPS induced lipid accumulation in the ovaries by promoting the transport of more lipids from the circulation to the ovaries and by upregulating triacylglycerol synthesis-related genes. Significantly increased expression of cpt2, acadm, acadl, and pparα, which are involved in ß-oxidation in the ovarian mitochondria, indicated that more energy was provided for oocyte maturation in exposed zebrafish ovaries. Thus, the proportion of full-grown stage oocytes in ovaries and egg reproduction were elevated at an accelerated rate, which earlier than normal reproductive cycle (8-10 days posts pawning). Moreover, the maternally BPS-exposed F1 embryos (2 h post-spawning, hpf) showed higher neutral lipid levels, impaired hatching capacity, and increased occurrence of larval deformities. All these findings demonstrated that stimulated lipid synthesis and ß-oxidation in zebrafish ovaries significantly contribute to BPS-induced oocyte precociousness with subsequent effects on the development of unexposed offspring. This study provides new insight into the impact of xenoestrogens on oviparous reproduction in females and offspring development from the perspective of ovarian lipid metabolism.

Unravelling metabolism and microbial community of a phytobed co-planted with Typha angustifolia and Ipomoea aquatica for biodegradation of doxylamine from wastewater.

Pharmaceutical contaminants in environment induce unexpected effects on ecological systems and human; thus, development of efficient technologies for their removal is immensely necessary. In this study, biodegradation and metabolic fate of a frequently found pharmaceutical contaminant, doxylamine by Typha angustifolia and Ipomoea aquatica was investigated. Microbial community of the plant rhizosphere has been identified to understand the important roles of the functional microbes. The plants reduced 48-80.5 % of doxylamine through hydrolysis/dehydroxylation and carbonylation/decarbonylation. A constructed phytobed co-planted with T. angustifolia and I. aquatica removed 77.3 %, 100 %, 83.67 %, and 61.13 % of chemical oxygen demand, total nitrogen, total phosphorus, and doxylamine respectively from real wastewater. High-throughput sequencing of soil and rhizosphere indicated that the phyla Proteobacteria, Bacteroidetes, Firmicutes, Planctomycetes, Actinobacteria, and Cyanobacteria dominated the microbial communities of the phytobed. Current study has demonstrated the applicability of the developed phytobeds for the treatment of doxylamine from municipal wastewater and provide a comprehensive understanding of its metabolism through plant and its rhizospheric microbial communities.

Biodegradation of Doxylamine From Wastewater by a Green Microalga, Scenedesmus obliquus.

Pharmaceutical contaminants (PCs) have been recognized as emerging contaminants causing unexpected consequences to environment and humans. There is an urgent need for development of efficient technologies to treat these PCs from water. The current study has investigated the removal capacity of a green microalgal species, Scenedesmus obliquus, for doxylamine, chemical oxygen demand (COD), and nutrients from real wastewater. Results have indicated that S. obliquus can grow well in the doxylamine-polluted wastewater with the achievement of 56, 78.5, 100, and 89% removal of doxylamine, COD, total nitrogen (TN), and total phosphorus (TP). Addition of 2 g L-1 bicarbonate enhanced the removal of doxylamine up to 63% and slightly inhibited the removal of COD. Decreased carbohydrate (28-26%) and increased protein content (30-33%) of the harvested biomass have been observed after cultivation in the wastewater. The current study has shown the feasibility of using microalgae-based biotechnologies for PC-contaminated wastewater.

Insights into the effect of cerium oxide nanoparticle on microalgal degradation of sulfonamides.

Nanoparticles have been commercially used worldwide; however, there is a lack of information of their environmental impacts and ecotoxicity. In this study, the effect of cerium oxide nanoparticle (CeO2NP) on a green microalga Scenedesmus obliquus, and microalgal biodegradation of four sulfonamides (sulfamethazine, sulfamethoxazole, sulfadiazine, and sulfamethoxazole) was investigated. There is insignificant inhibition of microalgal growth induced by CeO2NP; however, it substantially influenced the expression of genes involved in key cellular metabolic activities of S. obliquus. For example, genes involved in photosynthetic activity (psbA) and energy production (ATPF0C) were downregulated with exposure to CeO2NP. The low concentrations of CeO2NP improved microalgal degradation of sulfonamides. This may be because of the upregulated genes encoding hydrogenase and oxidoreductase. The exploration of this study has provided a new understanding of the environmental impacts of CeO2NP on microalgae-based biotechnologies for treatment of wastewater containing emerging organic contaminants.

Toxicity of benzophenone-3 and its biodegradation in a freshwater microalga Scenedesmus obliquus.

Environmental contamination by benzophenone-3 has gained attention because of its frequent occurrence and adverse environmental impact. Studies investigating the toxicity and removal mechanisms, along with its degradation pathway in microalgae are still rare. In this study, the ecotoxicity of benzophenone-3 on Scenedesmus obliquus was assessed through dose-response test, risk quotient evaluation, and changes of microalgal biochemical characteristics and gene expression. The calculated risk quotients of benzophenone-3 were >1, implying its high environmental risk. Expression of the ATPF0C and Tas genes encoding ATP-synthase and oxidoreductase was significantly increased in S. obliquus after exposure to benzophenone-3, while that of Lhcb1 and HydA genes was reduced. When exposed to 0.1-3 mg L-1 benzophenone-3, 23-29 % removal was achieved by S. obliquus, which was induced by abiotic removal, bioadsorption, bioaccumulation and biodegradation. Metabolic fate analyses showed that biodegradation of benzophenone-3 was induced by hydroxylation, and methylation, forming less toxic intermediates according to the toxicity assessment of the identified products. This study provides a better understanding of the toxicity and metabolic mechanisms of benzophenone-3 in microalgae, demonstrating the potential application of microalgae in the remediation of benzophenone-3 contaminated wastewater.

Plant and microalgae consortium for an enhanced biodegradation of sulfamethazine.

Pharmaceutical contamination in diverse water resources has been recognized as an emerging concern in environment because of its wide distribution and adverse effects on aquatic microorganisms and human health. Plant remediation with augmentation of microorganisms is a cost-effective and environmentally friendly approach toward an efficient treatment of pollutants, which can be easily applied in situ. (Bio)degradation of sulfamethazine (SMZ) by Iris pseudacorus, microalgal consortium, and plant-microalgal consortium was investigated. I. pseudacorus and microalgae could remove 63.5, and 25.8% of 1 mg SMZ L-1, respectively, whereas, the plant-microalgal consortium achieved 74% removal. The identified intermediates extracted after plant remediation indicated (bio)degradation of SMZ was through ring cleavage, hydroxylation, and dehydroxylation. Pigment content (total chlorophyll and carotenoid) of I. pseudacorus was significantly influenced by SMZ stress. A phytoreactor (20 L) constructed with I. pseudacorus achieved 30.0% and 71.3% removal of 1 mg SMZ L-1 from tap water and nutrient medium. This study has provided a better understanding of the metabolic mechanisms of SMZ in plants and showed the potential development of a plant-microalgal consortium as an advanced technology for treatment of these emerging contaminants. Graphical abstract.

Uptake and biodegradation of emerging contaminant sulfamethoxazole from aqueous phase using Ipomoea aquatica.

Plants serve as appropriate markers of worldwide pollution because they are present in almost every corner of the globe and bioaccumulate xenobiotic chemicals from their environment. The potential of a semi-aquatic plant, Ipomoea aquatica, to uptake and metabolize sulfamethoxazole (SMX) was investigated in this study. I. aquatica exhibited 100% removal of 0.05 mg L-1 SMX from synthetic media within 30 h. The I. aquatica achieved 93, 77 and 72% removal of SMX at 0.2, 0.5 and 1 mg L-1, respectively, after 48 h. This indicated that removal efficiency of I. aquatica was deteriorating at high concentrations of SMX. The chlorophyll and carotenoid content of I. aquatica was insignificantly influenced by SMX irrespective of its high concentration. Similarly, scanning electron microscopy (SEM) showed that exposure to SMX had an insignificant impact on morphology of the plant organelles. The mechanisms of removal by I. aquatica were explored by evaluating contributions of bioadsorption, bioaccumulation and biodegradation. There was negligible adsorption of SMX to plant roots. Accumulation of SMX within plant roots and stems was not observed; however, I. aquatica accumulated 17% of SMX in leaves. Thus, the major mechanism of elimination of SMX was biodegradation, which accounted for 82% removal of SMX. Gas chromatography-mass spectrometry (GC-MS) confirmed that I. aquatica biodegraded SMX into simpler compounds, and generated 4-aminophenol as its final product. A laboratory scale phytoreactor was used to investigate the application of I. aquatica in a simulated system, where it achieved 49% removal of SMX (0.2 mg L-1) in 10 d.