Cadmium absorption and translocation in rice plants are influenced by lower air temperatures during grain filling stage.

The increasing frequency and severity of low temperatures, and soil cadmium (Cd) pollution threaten food security. However, the interactive effects of Cd exposure and low temperatures on rice yield and quality, as well as the mechanisms of Cd absorption and translocation, remain unclear. In this study, two rice varieties were cultivated in soils with two Cd contamination levels (Cdhigh and Cdlow) and exposed to control (CT25) or lower temperatures of 20 °C (LT20) and 17 °C (LT17) during grain-filling stage. Results showed significant decreases in seed setting rate and grain weight, reduced head rice yield, and increased chalkiness due to low temperatures, particularly in Cdhigh soils. Compared to CT25, LT17 and LT20 increased Cd concentration by 37.6 % and accumulation by 14.8 % in grains grown in Cdhigh soils. Enhanced root activity and upregulation of OsNramp1 and OsNramp5 under both low-temperatures increased Cd levels in roots. Lower temperatures also decreased phytochelatins (PCs) and increased expression of OsHMA2 and OsCAL1, facilitating Cd transport and raising Cd levels in stems. Furthermore, upregulated OsHMA2, OsLCT1, and OsZIP7 in stems under low-temperatures promoted Cd transport to panicles. Overall, low temperatures during grain filling increased Cd uptake and translocation into rice grains, especially in high Cd contaminated soils, raising health risks. The study highlights the need to address climate change's impact on cadmium hazards in rice.

Bioengineering for robust tolerance against cold and drought stresses via co-overexpressing three Cu-miRNAs in major food crops.

Environmental stresses threaten global food security by reducing major crop productivity. MicroRNAs (miRNAs), a class of small non-coding RNAs, function as master regulators of gene expression in plants. In this study, we co-overexpressed three copper-miRNAs (miR397, miR408, and miR528) in three major food crops (referred to as 3miR-OE), which simultaneously silenced several target laccase genes, resulting in reduced lignin contents but increased flavonoid metabolites. Importantly, we observed that, compared to wild-type and single miRNA overexpression lines, the 3miR-OE transgenic Japonica and Indica rice exhibited significantly enhanced tolerance against cold and drought stresses throughout the growth period. In addition, 3miR-OE transgenic maize and wheat also exhibited robust resistance to cold and water-deficit conditions, suggesting that co-overexpressing three Cu-miRNAs is a powerful tool for improving resilience to abiotic stresses across diverse crops. Altogether, we have developed a bioengineering strategy to maintain crop growth and yield under unfavorable environmental conditions.

Androgen-ablative therapies inducing CXCL8 regulates mTORC1/SREBP2-dependent cholesterol biosynthesis to support progression of androgen receptor negative prostate cancer cells.

Treatment with androgen-ablative therapies effectively inhibited androgen receptor (AR)-positive (AR+) prostate cancer (PCa) cell subtypes, but it resulted in an increase in AR-negative (AR-) PCa cell subtypes. The present study aimed to investigate the debated mechanisms responsible for the changing proportion of cell types, identifying CXCL8 as a synthetic essential effector of AR- PCa cells. AR- PCa cells were found to be susceptible to CXCL8 depletion or inhibition, which impaired their survival. Mechanistically, androgen-ablative therapies resulted in the suppression of AR signaling, leading to the upregulation of CXCL8 gene transcription. CXCL8, in turn, activated the mTORC1 pathway, which increased de novo cholesterol synthesis by activating sterol regulatory element-binding protein-2 (SREBP2). Together, these results suggested that the CXCL8-mTORC1-SREBP2 axis contributed to the exacerbation of tumorigenicity in AR- PCa cells under androgen-ablative therapies.

Vancomycin-Loaded in situ Gelled Hydrogel as an Antibacterial System for Enhancing Repair of Infected Bone Defects.

Purpose: During treatment of infected bone defects, control of infection is necessary for effective bone repair, and hence controlled topical application of antibiotics is required in clinical practice. In this study, a biodegradable drug delivery system with in situ gelation at the site of infection was prepared by integrating vancomycin into a polyethylene glycol/oxidized dextran (PEG/ODEX) hydrogel matrix. Methods: In this work, PEG/ODEX hydrogels were prepared by Schiff base reaction, and vancomycin was loaded into them to construct a drug delivery system with controllable release and degradability. We first examined the microstructure, degradation time and drug release of the hydrogels. Then we verified the biocompatibility and in vitro ability of the release system. Finally, we used a rat infected bone defect model for further experiments. Results: The results showed that this antibacterial system could be completely biodegradable in vivo for 56 days, and its degradation products did not cause specific inflammatory response. The cumulative release of vancomycin from the antibacterial system was 58.3% ± 3.8% at 14 days and 78.4% ± 3.2% at 35 days. The concentration of vancomycin in the surrounding environment was about 1.2 mg/mL, which can effectively remove bacteria. Further studies in vivo showed that the antibacterial system cleared the infection and accelerated repair of infected bone defects in the femur of rats. There was no infection in rats after 8 weeks of treatment. The 3D image analysis of the experimental group showed that the bone volume fraction (BV/TV) was 1.39-fold higher (p < 0.001), the trabecular number (Tb.N) was 1.31-fold higher (p < 0.05), and the trabecular separation (Tb.Sp) was 0.58-fold higher than those of the control group (p < 0.01). Conclusion: In summary, this study clearly demonstrates that a clinical strategy based on biological materials can provide an innovative and effective approach to treatment of infected bone defects.

The Evolution and Biological Activity of Metazoan Mixed Lineage Kinase Domain-Like Protein (MLKL).

In mammals, mixed lineage kinase domain-like protein (MLKL) is the executor of necroptosis. MLKL comprises an N-terminal domain (NTD), which alone suffices to trigger necroptosis by forming pores in the plasma membrane, and a C-terminal domain that inhibits the NTD activity. Evolutionarily, MLKL is poorly conserved in animals and not found in Protostomia. Although MLKL orthologs exist in invertebrate Deuterostomia, the biological activity of invertebrate MLKL is unknown. Herein, we examined 34 metazoan phyla and detected MLKL not only in Deuterostomia but also in Protostomia (Rotifera). The Rotifera MLKL exhibited low identities with non-Rotifera MLKL but shared relatively high identities with non-metazoan MLKL. In invertebrates, MLKL formed two phylogenetic clades, one of which was represented by Rotifera. In vertebrates, MLKL expression was tissue-specific and generally rich in immune organs. When expressed in human cells, the MLKL-NTD of Rotifera, Echinodermata, Urochordata, and Cephalochordata induced strong necroptosis. The necroptotic activity of Rotifera MLKL depended on a number of conserved residues. Together these findings provided new insights into the evolution of MLKL in Metazoa and revealed the biological activity of invertebrate MLKL.

Diagnostic Value of Serum Apolipoprotein B100 Combined With Hippocampal Volume in Alzheimer's Disease.

PURPOSE: To explore the diagnostic value of serum apolipoprotein B100 (Apo B100) combined with hippocampal volume in Alzheimer's disease (AD). METHODS: A total of 59 AD patients and 59 healthy subjects were selected. The Mini-Mental State Examination (MMSE) was used for neuropsychological assessment. Blood glucose and serum lipid levels were detected by biochemical analyzer. Polymerase chain reaction (PCR) was used to detect apolipoprotein E (Apo E) ε3/ε4 genotypes in the plasma. Hippocampal volume was calculated using Slicer software. Independent-sample t test or Mann-Whitney U test were used to compare the levels of various indicators between the two groups. Spearman's correlation analysis was used to analyze the correlation between each level. The receiver operating characteristic curve (ROC) was plotted, and the area under the curve (AUC) was calculated to compare the diagnostic efficacy of individual and combined detection of serum Apo B100 levels and hippocampal volume in AD. RESULTS: Compared with the healthy control group, the levels of serum total cholesterol (TC), low-density lipoprotein (LDL), Apo B100, and plasma Apo E ε3/ε4 were higher in the AD group, and serum high-density lipoprotein (HDL) level was lower in the AD group (both p < 0.05). The hippocampal volume in the AD group was lower than in the control group (p < 0.01). The serum Apo B100 level was negatively correlated with MMSE score (r = -0.646), whereas hippocampal volume was positively correlated with MMSE score (r = 0.630). ROC curve analysis showed that the AUC of the combined serum Apo B100 level and hippocampal volume for AD was higher than that of either alone (AUC = 0.821, p < 0.01). CONCLUSION: Serum Apo B100 level is elevated, and the hippocampal volume is reduced in AD patients. The combined detection of the two has a higher diagnostic efficiency for AD than other alone and has the potential to become an important indicator for the diagnosis of AD in the future.

CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost.

Caspase 9 (CASP9) is a well-known initiator caspase of intrinsic apoptosis. In humans, cIAP1 binds and induces degradation of the activated form of CASP9, but not pro-CASP9. In fish, the activity and regulation of CASP9 remain unknown. In this work, using flounder Paralichthys olivaceus as a representative species, we examined the regulatory mechanism of CASP9 in teleost. P. olivaceus CASP9 (PoCASP9) induced robust apoptosis, which was inhibited by P. olivaceus cIAP1 (PocIAP1). Unlike human cIAP1, PocIAP1 bound both pro- and active PoCASP9 and induced their degradation via the RING domain-involved proteasome pathway. In humans, the adaptor molecule CRADD cannot interact with CASP9. In contrast, P. olivaceus CRADD (PoCRADD) bound both pro- and active PoCASP9 via CARD-CARD interaction and enhanced apoptosis by promoting the cellular levels of pro- and active PoCASP9. Furthermore, PoCRADD abrogated the inhibition of PoCASP9 by PocIAP1 by preventing PocIAP1-PoCASP9 interaction. Together these results reveal a CASP9 regulation mechanism in teleost that differs from that in humans and demonstrate that teleost CASP9 is tightly and directly controlled by both negative and positive regulators that exert a regulation effect both before and after CASP9 activation. These findings advance our understanding of the regulation of CASP9-mediated apoptosis in vertebrates.

Fabrication of a ZIF-8/PVA Membrane on PVDF Fiber by Spray for the Highly Efficient Separation of Oil-in-Water Emulsions.

In human life and production, excessive oily wastewaters containing detergents are produced and discharged, causing severe environmental pollution and water resource problems. A metal-organic framework (MOF)-based membrane is an economical and environmentally friendly tool for emulsion separation but is limited by a complex preparation process and poor flexibility. Herein, we developed a simple method to synthesize a MOF-based mixed-matrix membrane (MMM) by spray and fabricated the membrane on poly(vinylidene fluoride) (PVDF) fibers via postdeposition and in situ growth manners. The prepared ZIF-8/PVA MMMs have uniform distribution of ZIF-8 particles and poly(vinyl alcohol) (PVA) on the PVDF fibers. PVA improved the adhesion between the ZIF-8 particles and between the MOF layer and PVDF fiber, endowing the separation membrane with high flexibility and bendability. The prepared ZIF-8/PVA membrane exhibited hydrophilicity and underwater superoleophobicity, which showed high separation efficiency and considerable water flux for emulsions, emulsion containing dye, and surfactant-stabilized emulsion. In addition, the fabricated ZIF-8/PVA/PVDF fibers exhibited good antifouling property and flexibility and can maintain stable separation efficiency after working several times and even under bending, demonstrating the stability and potentiality of the ZIF-8/PVA/PVDF fibers in practical applications. This work paves a new avenue for the synthesis and application of MOF-based MMMs.

Mapping annual dynamics of surface mining disturbances in the northeastern Tibetan Plateau using Landsat imagery and LandTrendr algorithm.

The exploitation and utilization of coal resources have significantly contributed to global energy security. However, this mining activity has inflicted considerable damage on the ecological environment, particularly on the Tibetan Plateau, where the impact on ecosystems may be even more detrimental. The implementation of high-intensity mining activities leads to rapid changes in land cover/land use. Consequently, it is essential to accurately and effectively monitor mining disturbances. In this study, we propose an approach to capture surface mining disturbances using spatial-temporal rules and time series stacks of Landsat data. First, a time series of annual mining disturbance probability was generated based on Landsat temporal-spectral metrics and random forest. Second, the Landsat-based detection of Trends in Disturbance and Recovery (LandTrendr) algorithm was employed to segment the time series and detect breakpoints. Finally, mining disturbances were captured by further restricting the output of LandTrendr based on spatial-temporal rules of mining disturbances. This approach was applied and evaluated in the Muli mining area of the northeastern Tibetan Plateau, which experienced large-scale and rapid mining disturbances from 2004 to 2014, and identified a disturbed mining area of 43.62 km2. The mining sites have been reclaimed after mining, and all reclamation work was done after 2016, with a total reclaimed area of 22.28 km2. The validation results indicated that the overall accuracy of mining disturbance and reclamation mapping ranges from 0.7333 to 0.8667, and the F1 scores for mining disturbances and reclamation range from 0.7551 to 0.8723. This study provides a reliable framework for monitoring mining disturbances and reclamation in surface mines, promising to be useful in realizing disturbance monitoring in surface mines for a wide range of mineral types.

Room-temperature ferroelectric, piezoelectric and resistive switching behaviors of single-element Te nanowires.

Ferroelectrics are essential in memory devices for multi-bit storage and high-density integration. Ferroelectricity mainly exists in compounds but rare in single-element materials due to their lack of spontaneous polarization in the latter. However, we report a room-temperature ferroelectricity in quasi-one-dimensional Te nanowires. Piezoelectric characteristics, ferroelectric loops and domain reversals are clearly observed. We attribute the ferroelectricity to the ion displacement created by the interlayer interaction between lone-pair electrons. Ferroelectric polarization can induce a strong field effect on the transport along the Te chain, giving rise to a self-gated ferroelectric field-effect transistor. By utilizing ferroelectric Te nanowire as channel, the device exhibits high mobility (~220 cm2·V-1·s-1), continuous-variable resistive states can be observed with long-term retention (>105 s), fast speed (<20 ns) and high-density storage (>1.92 TB/cm2). Our work provides opportunities for single-element ferroelectrics and advances practical applications such as ultrahigh-density data storage and computing-in-memory devices.

CO-Free Aminocarbonylation of Terminal Alkynes Catalyzed by Synergistic Effect From Metal-Organic Frameworks.

Incorporation of CO into substrates to construct high-value carbonyl compounds is an intensive industrial carbonylation procedure, however, high toxicity and wide explosion limits (12.5-74.0 vol% in air) of CO limit its application in industrial production. The development of a CO-free catalytic system for carbonylation is one of ideal methods, but full of challenge. Herein, this study reports the CO-free aminocarbonylation conversion of terminal alkynes synergistically catalyzed by a unique Co(ІІ)/Ag(І) metal-organic framework (MOF), in which the combination of isocyanides and O2 is employed as safe and green source of aminocarbonyl. This reaction has broad substrate applicability in terminal alkyne and isocyanides components with 100% atom economy. The bimetal MOF catalyst can be recycled at least five times without substantial loss of catalytic activities. Mechanistic investigations demonstrate that the synergistic effect between Ag(I) and Co(II) sites can efficiently activate terminal alkyne and isocyanides, respectively. Free radical capture experiments, FT-IR analysis and theoretical explorations further reveal that terminal alkynes and isocyanides can be catalytically transformed into an anionic intermediate through heterolysis pathways. This work provides secure and practical access to carbonylation as well as a new approach to aminocarbonylation of terminal alkynes.

Microalgae treatment of food processing wastewater for simultaneous biomass resource recycling and water reuse.

Food processing wastewater presents a considerable challenge for treatment owing to its elevated nitrogen and phosphorus levels. Nonetheless, it possesses inherent value attributed to its abundant nutrients and organic content. This study presents an innovative approach for treating food processing wastewater and reusing biomass. Initially, the secondary-treated wastewater undergoes flocculation and sedimentation, followed by reverse osmosis to ensure that the effluent meets reuse standards. Subsequently, reverse osmosis concentrates, generated at varying water recovery rates, are utilized for microalgae cultivation to recover nitrogen and phosphorus. Furthermore, this study highlights the potential of reverse osmosis concentrates in reducing the water demand for microalgae cultivation and in producing commercial-grade nutrients. The findings reveal that reverse osmosis achieves removal rates exceeding 90 % for both nitrogen and phosphorus and effluent meets reuse standards. Following seven days of cultivation, microalgae cultured in reverse osmosis concentrated water with an 80 % water recovery rate demonstrate denitrification and phosphorus removal rates of 73.88 % and 80.92 % respectively, with a biomass concentration of 563 mg/L and a protein yield of 128 mg/L. Moreover, a total volumetric energy yield of 10.08 kJ/L is obtained, facilitating energy valorization. In conclusion, this study offers practical solutions for wastewater treatment and resource recovery, enabling the attainment of zero discharge of pollutants while generating valuable resources through microalgae cultivation.

Efficacy and safety profile of doxofylline in asthma: a meta-analysis.

OBJECTIVE: This work aims to explore the effectiveness and safety of doxofylline in asthma treatment. DATA SOURCES: Relevant studies published before March 2023 were retrieved from EMBASE, Cochrane Library, PubMed, and Web of Science databases. STUDY SELECTIONS: Risk of Bias tool (RoB 2) was applied to determine the quality of randomized controlled trials (RCTs). Relative risks (RR, 95% confidence intervals [CI]) and weighted mean differences (WMD, 95% CI) were calculated for dichotomous and continuous outcomes, respectively, under fixed or random-effects models. RESULTS: A total of eight clinical trials comprising 1627 patients were analyzed. The meta-analysis revealed no notable change in forced expiratory volume in 1 s (FEV1) (WMD = 0.48; 95% CI: -2.09 to 3.05), the use of albuterol as a rescue medication (WMD = -0.02; 95% CI: -0.57 to 0.52), forced vital capacity (FVC) (WMD = 0.19; 95% CI: -0.28 to 0.67) and FEV1 predicted value (WMD = 1.53; 95%CI: -0.88 to 3.94) between doxofylline and control groups. However, doxofylline treatment significantly reduced adverse reactions (RR = 0.71; 95% CI: 0.60 to 0.84) and decreased the incidence of asthma events (WMD = -0.18; 95% CI: -0.33 to 0.03). Subgroup analysis results indicated that the improvement in FEV1 with doxofylline combined with budesonide was superior to that of budesonide combined with montelukast or tiotropium but inferior to that of budesonide plus formoterol combination. CONCLUSION: Doxofylline treatment significantly reduces the risk of asthma events and adverse events (AEs), demonstrating good safety and longer-term benefits.

USP22 promotes gefitinib resistance and inhibits ferroptosis in non-small cell lung cancer by deubiquitination of MDM2.

BACKGROUND: The emergence of chemoresistance markedly compromised the treatment efficiency of human cancer, including non-small cell lung cancer (NSCLC). In the present study, we aimed to explore the effects of ubiquitin-specific peptidase 22 (USP22) and murine double minute 2 (MDM2) in gefitinib resistance in NSCLC. METHODS: Immunohistochemistry (IHC) assay, quantitative real-time polymerase chain reaction (qRT-PCR) assay and western blot assay were carried out to determine the expression of USP22 and MDM2. Transwell assay and flow cytometry analysis were performed to evaluate cell migration and apoptosis. Cell Counting Kit-8 (CCK-8) assay was employed to assess gefitinib resistance. The phenomenon of ferroptosis was estimated by related commercial kits. The oxidized C11-BODIPY fluorescence intensity by C11-BODIPY staining. The relation between USP22 and MDM2 was analyzed by ubiquitination assay and co-immunoprecipitation (Co-IP) assay. RESULTS: USP22 was abnormally upregulated in NSCLC tissues and cells, and USP22 silencing markedly repressed NSCLC cell migration and facilitated apoptosis and ferroptosis. Moreover, our results indicated that ferroptosis could enhance the suppressive effect of gefitinib on NSCLC cells. Besides, USP22 overexpression enhanced gefitinib resistance and ferroptosis protection in NSCLC cells. Mechanically, USP22 stabilized MDM2 and regulated MDM2 expression through deubiquitination of MDM2. MDM2 deficiency partially restored the effects of USP22 on gefitinib resistance and ferroptosis in NSCLC cells. Of note, we validated the promotional effect of USP22 on gefitinib resistance in NSCLC in vivo through establishing the murine xenograft model. CONCLUSION: USP22/MDM2 promoted gefitinib resistance and inhibited ferroptosis in NSCLC, which might offer a novel strategy for overcoming gefitinib resistance in NSCLC.

Influence of perfluorooctanoic acid on alkaline anaerobic fermentation of waste activated sludge: Perspective from volatile fatty acids production and sludge reduction.

Alkaline anaerobic fermentation is an effective approach for resource utilization and reduction of waste activated sludge (WAS). Perfluorooctanoic acid (PFOA) is widespread in WAS, however, its potential impact on alkaline anaerobic fermentation of WAS remains largely unknown. Hence, this study focused on investigating the influence of PFOA on volatile fatty acids (VFAs) production and sludge reduction during alkaline anaerobic fermentation (pH = 10 ± 0.1), as well as the critical mechanisms. Results demonstrated that low PFOA concentration (5 mg/kg-TS) raised VFAs yield to 109.37%, while high levels of PFOA (25 and 50 mg/kg-TS) remarkably decreased VFAs production to 89.55% and 80.44% of the control. Mechanism exploration revealed that PFOA facilitated the solubilization process, and low PFOA level enhanced the accumulation of VFAs via increased bioavailable substrates and the activities of enzymes and microorganisms. On the contrary, high levels of PFOA were substantial biotoxicity, inducing excessive ROS production, causing oxidative damage, and reducing enzyme activity and functional microbial abundance, thereby decreasing VFAs production. Additionally, further analysis of sludge physicochemical properties confirmed that the effect of PFOA on WAS reduction exhibited the same trend as VFAs production. This work provides a basis for PFOA environmental risk assessment and WAS resource utilization.

Designing a flower-shaped ZnS/CoS heterojunction for efficient electroreduction of N2 to NH3.

The electrocatalytic nitrogen reduction reaction (NRR) is a highly promising process for synthesizing ammonia and holds great potential to replace the traditional Haber-Bosch process. Here, we report a novel flower-shaped ZnS/CoS composite electrocatalyst for the NRR. Remarkably, the ZnS/CoS-105 heterojunction catalyst achieved an NH3 yield rate of 20.42 µg h-1 mgcat.-1 and a faradaic efficiency (FE) of 11.83% at -0.45 V VS. RHE in an aqueous 0.1 M Na2SO4 solution. In addition, ZnS/CoS-105 showed remarkable stability (up to 24 h) for the NRR process.

Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens.

Gasdermins (GSDMs) are proteins cleaved by caspase (CASP) to trigger pyroptosis. In teleosts, pyroptosis is mediated by gasdermin E (GSDME). The Pufferfish, Takifugu rubripes, possesses two GSDME orthologs: named TrGSDMEa and TrGSDMEb. TrGSDMEa is cleaved by CASP3/7 to liberate the N-terminal (NT) domain that can trigger pyroptosis in mammalian cells. However, the biological function of TrGSDMEa in pufferfish is unknown, and TrGSDMEb is poorly studied. We found that TrGSDMEb was cleaved by CASP1/3/6/7/8, but the resulting NT domain, despite its similarity to TrGSDMEa-NT domain in sequence and structure, failed to induce pyroptosis. TrGSDMEa and TrGSDMEb exhibited similar expression patterns in pufferfish under normal physiological conditions but were up- and downregulated, respectively, in expression during Vibrio harveyi and Edwardsiella tarda infection. Bacterial infection induced the activation of TrGSDMEa and CASP3/7 in pufferfish cells, resulting in pyroptosis accompanied with IL-1ß production and maturation. Inhibition of TrGSDMEa-mediated pyroptosis via TrCASP3/7 reduced the death of pufferfish cells and augmented bacterial dissemination in fish tissues. Structure-oriented mutagenesis identified 16 conserved residues in teleost GSDMEa that were required for the pore formation or auto-inhibition of GSDMEa. This study illustrates the role of GSDMEa-mediated pyroptosis in teleost defense against bacterial pathogens and provides new insights into the structure-based function of vertebrate GSDME. Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-024-00237-x.

Photoinduced Deoxygenative C2 Arylation of Quinoline N-oxides with Phenylhydrazines to 2-Arylquinolines over Porous Tubular Graphitic Carbon Nitride Semiconductor Photocatalyst.

The photo-induced deoxygenative C2 arylation of quinoline N-oxides to 2-arylquinolines is achieved over a heterogeneous porous tubular graphitic carbon nitride (PTCN) catalyst with phenylhydrazines as arylation reagent. A wide range of quinoline N-oxides can be efficiently transformed into their corresponding 2-arylquinolines under visible light irradiation. Moreover, PTCN catalyst is easily separated and could be reused several times without loss to its original activity.

Incident Angle Sensing and Adaptive Control of Scattering by Intelligent Metasurface.

The passive sensing and active control of electromagnetic (EM) waves have always been attractive in electronic and information areas, especially during the intelligent era. Here a new method is presented to achieve the angle sensing of incident wave and adaptive control of backward scattering using the intelligent metasurface. The proposed unit cells have the ability to dynamically manipulate the receiving and reflection of the EM energy respectively. The angle sensing of incident waves can be actualized using the method of compressive sensing based on multiple receiving patterns, which are generated by randomly switching the receiving and reflection states of the unit cells. Afterward, the customized performances of backward scattering waves according to the cognitive incident angle can be realized by controlling the programmable reflective phases of unit cells correspondingly. One prototype composed of the metasurface and the module for sensing and adaptive feedback control is fabricated. The whole intelligent metasurface with customizing the function of retro-reflection or low scattering is measured without human intervention and the good results acquired can verify the validity of the proposed concept and design.

Unraveling phytoremediation mechanisms of the common reed (Phragmites australis) suspension cells towards ciprofloxacin: Xenobiotic transformation and metabolic reprogramming.

Phytoremediation is an effective solution to treat pollution with antibiotic compounds in aquatic environments; however, the underlying mechanisms for plants to cope with antibiotic pollutants are obscure. Here we used cell suspension culture to investigate the distribution and transformation of ciprofloxacin (CIP) in common reed (Phragmites australis) plants, as well as the accompanying phenotypic and metabolic responses of plants. By means of radioactive isotope labelling, we found that in total 68 % of CIP was transformed via intracellular Phase I transformation (reduction and methylation), Phase Ⅱ conjugation (glycosylation), and Phase Ⅲ compartmentalization (cell-bound residue formation mainly in cell walls, 23 %). The reduction and glycosylation products were secreted by the cells. To mitigate stress induced by CIP and its transformation products, the cells activated the defense system by up-regulating both intra- and extra-cellular antioxidant metabolites (e.g., catechin, l-cystine, and dehydroascorbic acid), anti-C/N metabolism disorder metabolites (e.g., succinic acid), secreting signaling (e.g., nicotinic acid), and anti-stress (e.g., allantoin) metabolites. Notably, the metabolic reprogramming could be involved in the CIP transformation process (e.g., glycosylation). Our findings reveal the strategy of wetland plants to cope with the stress from CIP by transforming the xenobiotic compound and reprogramming metabolism, and provide novel insights into the fate of antibiotics and plant defense mechanisms during phytoremediation.